cellular pathology techniques educational workshops brochure 2016...delegates. the workshop is based...

UK NEQAS for Cellular Pathology Technique |www.ukneqascpt.org.uk |Email: [email protected] |The Pathology

Centre, Queen Elizabeth Hospital, Sheriff Hill, Gateshead, Tyne and Wear NE9 6SX |Tel: +44 (0)191 445 5566

Cellular Pathology Techniques

Educational Workshops

UK NEQAS for Cellular Pathology Technique

Providing worldwide external quality assessment and proficiency testing

for all aspects of tissue diagnostics

2 | P a g e Providing worldwide external quality assessment and proficiency testing for all aspects of tissue diagnostics

Worldwide Participation in External Quality Assessment

and Proficiency Testing. UK National External Quality

Assessment Service fo r C e l l u l a r P a th o l o gy T e c h n i q u e s

( U K NE Q AS fo r C PT ) is an international “not for profit”

organization providing a comprehensive range of accredited

external quality assessment and proficiency testing

programmes for all aspects of tissue diagnostics

Tissue diagnostics is an extremely important element

in the management of patients and therefore the role of

external quality assessment is essential in order to

provide assurance that a high standard of testing is

achieved for all. Errors with tests can have catastrophic

results so it is important for UK NEQAS for CPT to ensure

early detection of problems and provide education, help

and support to organisations

Specialist Schemes and education are offered by UK NEQAS for CPT in the following;

� General Pathology (Routine Histopathology)

� Neuropathology

� Renal Biopsy Pathology

� Muscle Histochemistry

� Non Gynae Diagnostic Cytology

� Supplementary Specialisms

Education is important to provide useful information

that allow for quality improvement in Cellular Pathology

Techniques. UK NEQAS for CPT provides a large

educational component including; an educational

website, best methods and image galleries, annual

participant feedback meetings, scientific meetings and

seminars, newsletters and educational workshops


Special Staining Beginners / Refresher

Theory & Practice

June 2016 – book now

This introductory workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which

boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and

workshop delegates. The workshop will provide a basic introduction and training in the theory and practice in the use

of tinctorial stains used in cellular pathology laboratories, through the use of presentations, discussion and laboratory

based practical sessions.

On completion of the workshop students will have a basic knowledge of

• Theory of haematoxylin and eosin staining

• Assessment of staining quality

• Formalin fixation

• The use of control sections

• Classification of dyes

• Tissue artefacts

• Recognition of special stains

• The rationale of special staining techniques and their use in diagnostic histopathology

On completion of the workshop students will be able to

• Recognise a well stained H&E

• Identify and assess special stains

• Recognise section artefacts

• Understand how tissue artefacts are caused and how they may be eliminated or minimised

• Have an understanding of the classification and molecular structure of dyes

• Have an understanding of the rationale of dye staining mechanisms and associated problems

• Assess the quality of their own depts. stained preparations using UK NEQAS for CPT assessment criteria

• Assess adequacy of formalin fixation

Is this workshop the right one for me? This workshop is suitable for anyone who has a basic science background and wishes to gain an understanding of

theory and practice in cellular pathology techniques. The workshop will provide the knowledge for participants to

recognise, assess and trouble-shoot both haematoxylin and eosin stained sections together with the special staining

techniques used in diagnostic cellular pathology. Refreshments and Lunch will be provided.

This course is accredited by the Institute of Biomedical Science


Specialist Demonstration Techniques A

Theory & Practice June 2016 – book now

This specialist workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which boasts a

purpose built cellular pathology laboratory as part of their state of the art facilities for students and workshop

delegates. The workshop is based on the competencies required of the IBMS Cellular Pathology Specialist Portfolio

sections 7.2 a, b, c and d.

The workshop teaches an in depth knowledge of H&E staining and extracellular proteins such as fibrin and amyloid

and the application and principles of carbohydrate and mucin classification and demonstration, through the use of

presentations, discussion and laboratory based practical sessions.


• Haematoxylin and eosin staining

• Mordants

• Fibrin and its demonstration

• Classification of amyloid

• Principles and practice of routine staining methods for the demonstration of fibrin and amyloid

• Pathophysiology of fibrin and amyloid

• EQA of fibrin and amyloid

• Classification of carbohydrates

• Principles and practice of routine staining and impregnation methods for carbohydrate demonstration

• Principles of the pathophysiology of carbohydrate formation & pathological significance of

• EQA assessment of stained sections



• Use haematoxylin for various demonstration procedures

• Understand the advantages, disadvantages and use of haematoxylins made with different mordants

• Understand the process of progressive and regressive staining

• Understand the principles of haematoxylin and eosin staining and its value as a primary diagnostic stain

• Understand how to monitor the quality of H&E staining using standard criteria

• Assess stained sections for quality

• Be able to demonstrate extracellular proteins in tissue sections

• Understand the principles and practice of routine staining methods for extracellular protein demonstration

• Know the pathological significance of amyloid and fibrin and the techniques used in their demonstration

• Be able to demonstrate a range of carbohydrates in tissue sections

• Identify stained carbohydrate present in different tissues

Is this workshop the right one for me? This workshop is suitable for those students and laboratory personnel completing the IBMS Specialist Portfolio in

Cellular Pathology. The workshop will provide the knowledge for participants to recognise, assess and trouble-shoot

both haematoxylin and eosin stained sections together with the special staining techniques used in diagnostic cellular

pathology for extracellular proteins such as fibrin and amyloid, as well as the application and principles of

carbohydrate and lipid classification and demonstration.

Refreshments and Lunch will be provided.



Specialist Demonstration Techniques B

Theory & Practice August 2016 – book now



delegates. The workshop is based on the competencies required of the IBMS Cellular Pathology Specialist Portfolio

sections 7.2 e, f and g.

The workshop teaches an in depth knowledge of the application and principles of lipid classification and

demonstration, the types and demonstration of common infective agents, and pigments and minerals, through the

use of presentations, discussion and laboratory based practical sessions.


• Principles and practice of routine staining methods for the demonstration of Lipids, Infective agents,

pigments and minerals

• Lipid storage diseases

• Types of infective agents most commonly seen and the pathological significance of infective agent

demonstration

• The principles of the pathophysiology of pigment and mineral formation and the types of pigment and

mineral normally deposited in tissue

• How to review cases and material microscopically for internal and external quality control



• Demonstrate lipid in sections

• Understand the principles and practice of routine staining and histochemical methods for the demonstration

of different lipids

• Know how to review cases and material microscopically for internal and external quality control

• Be able to demonstrate common infective reagents in tissue sections

• Know the principles and practice of routine staining and impregnation methods for the demonstration of

infective agents

• Be able to demonstrate pigments and minerals in tissue sections

• Know the principles and practice of direct visualisation, routine staining and impregnation methods for the

demonstration and identification of pigments and minerals

Is this workshop the right one for me? This workshop is suitable for those students and laboratory personnel completing the IBMS Specialist Portfolio in

Cellular Pathology. The workshop will provide the knowledge for participants to recognise, demonstrate and review

special stained sections for special staining techniques used in diagnostic cellular pathology, including lipid expression

and its significance, common infective agents and their diagnostic implications, as well as the formation of pigments

and minerals found in normal tissues and clinical conditions.




Electron Microscopy & Neuropathology


This Introductory workshop is run as a half-day session in conjunction with The Principles of Muscle Biopsy at Buxton

Labs based in The Walton Centre NHS Foundation Trust, Liverpool, the only specialist hospital trust in the UK

dedicated to providing comprehensive neurology, neurosurgery, spinal and pain management services. The workshop

provides a basic introduction to the theory and application of electron microscopy in diagnostic neuropathology


• How an electron microscope works

• Sample preparation including fixation and ultra-microtomy

• Applications in Neuropathology (nerve biopsy, muscle biopsy and pituitary tumours)

• Principles of nerve biopsy

• Preparation and staining of Nerve Biopsy

On completion of the workshop students will have experienced

• Preparation of glass knives for ultra-microtomy

• Preparation of resin blocks for semi thin sections

• Cut, mount & stain a semi thin section

• QC sections under a light microscope


theory and practical experience in current electron microscopy, techniques, and those students & laboratory

personnel completing the IBMS Specialist Portfolio in Cellular Pathology. It will consist of a series of lectures,

demonstrations and some limited practical experience. The session is taught over half a day, with the other half

comprising the Principles of Muscle Biopsy. Refreshments and Lunch will be provided.



The Principles of Muscle Biopsy


This Introductory workshop is run as a half-day session in conjunction with Electron Microscopy and Neuropathology

at Buxton Labs based in The Walton Centre NHS Foundation Trust, Liverpool, the only specialist hospital trust in the

UK dedicated to providing comprehensive neurology, neurosurgery, spinal and pain management services. The

workshop provides a basic introduction to the structure of muscle and the theory and preparation of muscle biopsy.

On completion of the workshop students will understand

• Types of muscle

• The structure of muscle

• Theory and application of Muscle Biopsy

• Orientation of muscle tissue

• Cutting and staining of muscle tissue

On completion of the workshop students will have experienced

• Orientation of muscle tissue, (using a piece of steak)

• Visualising stained muscle sections using Light Microscopy


theory and practical experience in the principles of Muscle Biopsy and those students & laboratory personnel

completing the IBMS Specialist Portfolio in Cellular Pathology. It will consist of a series of lectures and a lab based

practical session and is taught over half a day, with the other half comprising Electron Microscopy and

Neuropathology. Refreshments and Lunch will be provided.



Immunocytochemistry Beginners / Refresher

Theory & Practice April & Sept 2016 – book now

This introductory workshop is run as a full day at The University of Northumbria in Newcastle Upon Tyne, which

boasts a purpose built cellular pathology laboratory as part of their state of the art facilities for students and

workshop delegates. The workshop will provide a basic introduction and training in immunocytochemistry theory,

practice and techniques used in cellular pathology laboratories, through the use of presentations, discussion and

laboratory based practical sessions.


• History and background of ICC staining

• Immunofluorescence

• Development of ICC techniques (Direct, Indirect, PAP, APAAP, streptavidin/biotin, ABC)

• Extended polymer labelled detection methods

• Chromogens and substrates

• Use of positive and negative controls

• Use of blocking serums

• Background staining

• Antibody titre

• Antigen retrieval techniques

• EQA of ICC within the clinical environment



• Understand the principles of immunocytochemistry

• Understand the rationale of immunocytochemical reactions

• Identify positive staining in tissue sections

• Identify good, poor and indifferent immunocytochemical staining

• Identify and understand the causes of background staining and how to eliminate it

• Explain the use of control sections and their importance

• Use antibodies and reagents following immunocytochemical staining protocols

• Understand the importance of correct antibody titre

• Understand the principles of antigen retrieval


theory and practice in immunocytochemistry techniques and those students & laboratory personnel completing the

IBMS Specialist Portfolio in Cellular Pathology. The workshop will provide the knowledge for participants to

recognise, assess and trouble-shoot both immunocytochemistry stained sections together with other techniques

used in diagnostic cellular pathology. Refreshments and Lunch will be provided.



Immunocytochemistry Intermediate / Troubleshooting

Theory & Practice April & September 2016 – book now



delegates. The workshop will provide an in depth focus on immunocytochemistry, its applications and

troubleshooting within cellular pathology laboratories, through the use of presentations, discussion and practical

based sessions.


• Diagnostic antibody utility in conjunction with modern detection and visualisation technologies

• The development and role of automation within the clinical environment

• Factors effecting instrument selection

• Safeguards to ensure robust clinical services

• The rationale behind visualisation techniques

• The benefits of the use of different detection systems and visualisation techniques

• Pitfalls and common issues in immunocytochemistry

• Troubleshooting and eliminating or minimising errors

• Application of detection and visualisation methods as prognostic indicators

• Key clinical conditions where these demonstration methods may be of diagnostic value

• The value of panels in immunocytochemistry for the diagnosis of disease

• EQA of ICC within the clinical environment



• Select the appropriate antibodies for the demonstration of components of the commonly stained tissues

• Identify positive staining

• Clearly distinguish between positive, negative and equivocal results

• Resolve problems associated with detection and visualisation methods

• Evaluate the staining of new antibodies and methodologies

• Assess the quality of stained slides

• Assess stained slides for artefacts

• Identify and understand the causes of artefacts and how to eliminate them

• Apply detection and visualisation methods as prognostic indicators

• Participate in the submission and evaluation of relevant EQA results

Is this workshop the right one for me? This workshop is suitable for anyone who has a specialist science background and wishes to gain an in depth

understanding of immunocytochemistry, its applications and troubleshooting within cellular pathology laboratories

and those students & laboratory personnel completing the IBMS Specialist Portfolio in Cellular Pathology. The

workshop will provide the specialist knowledge for participants to recognise, assess and trouble-shoot both

immunocytochemistry stained sections for diagnostic cellular pathology.


Delegates are invited to bring their own slides for troubleshooting if desired.



Non Gynae Cytology Beginners / Refresher

Theory & Practice May & August 2016 – book now

The introductory workshop is run as a full day at the Queen Elizabeth Hospital Gateshead. The workshop provides a

basic introduction to non gynae cytology, principles of preparation and cell morphology through the use of

presentations, discussion and practical microscopy sessions.


• Types of non gynae sample and where they come from

• Principles of non gynae preparation

• Quality including NEQAS scheme and assessment

• Troubleshooting

• Expected cells in benign samples

• Frequently seen malignancies in non gynae samples

On completion of the workshop students will have experienced through practical microscopy sessions

• Morphology of benign and malignant cells in non gynae samples

• Quality assessment and NEQAS criteria

• Troubleshooting specific preparation and staining problems

Is this workshop the right one for me? This workshop will give a basic overview of non gynae cytology, and will look at sample types, preparation methods,

cell morphology and quality issues. It is intended for those who are new to non gynae preparation and pre-screening.

It may be particularly useful for those who prepare non gynae samples but do not get the opportunity to pre-screen

or for those who have recently taken over non gynae cytology services as a result of laboratory reconfiguration.



Registration Form Cellular Pathology Education Workshop

*******PLEASE COMPLETE AND RETURN ASAP TO SECURE YOUR PLACE ******

Submission of this completed Registration Form is essential to secure a place on the course.

ALL SECTIONS MUST BE COMPLETED IN FULL

All workshops are held at the University of Northumbria University, UK* *With the exception of Neuro / Muscle workshops which will be held at the Walton Centre, Liverpool, and

the Non Gynae Cytology Beginners / Refresher workshop which will be held at QEH, Gateshead.

Workshops available Please indicate below, in the appropriate box, which workshops you wish to register for.

Please note if booking more than one place on the same workshop you will need to provide us

with a separate Registration Form for each individual.

The details of the individual attending the workshop must be completed in the “Required

Information for Booking Section”, overleaf.

Workshop Title

Date

Cost

Attending

(please tick as

appropriate)

Special Staining Beginners /

Refresher 22nd June 2016 £90 + VAT

Specialist Workshop A

23rd June 2016 £110 + VAT

Specialist Workshop B

11th August 2016 £110 + VAT

Non Gynae Cytology Beginners /

Refresher 5th May 2016 £90 + VAT

Non Gynae Cytology Beginners /

Refresher 10th August 2016 £90 + VAT

Muscle / Neuropathology and

Electron Microscopy

Introductory

15th June 2016 £110 + VAT

Muscle / Neuropathology and

Electron Microscopy

Introductory

16th June 2016 £110 + VAT

ICC Beginners / Refresher

6th April 2016 £90 + VAT

ICC Intermediate

7

th April 2016 £110 + VAT

ICC Beginners / Refresher

7th Sept 2016 £90 + VAT

ICC Intermediate

8

th Sept 2016 £110 + VAT






Accommodation Please note this is not a residential course, but accommodation may be provided at an additional

supplement of £85 per night B&B.

Please indicate below, in the appropriate box, if you do or do not require accommodation.

If accommodation is required please indicate, in the appropriate box, if accommodation is

required for the night before or after the workshop, or both.

I do not require accommodation

I will require accommodation Night prior to the workshop

Night following the workshop

Dietary requirements Price per person includes lunch and refreshments. If you have any special dietary requirements

please indicate below, in the appropriate box, and we will do our best to accommodate them.

YES ( please give details) No

Required Information for booking Please complete all of the required details below.

Your registration form will be returned if sections of this form are left blank and a place will not

be reserved for you.

PURCHASE ORDER (PO) NUMBER

Please provide for invoicing for payment (please indicate in

the box opposite)

UK NEQAS for CPT Participation Number

Please indicate your Participation Number if your centre is

subscribed to the Scheme (you must complete this to enable

us to register you on a workshop)

Please note, whilst we do understand the delays which may be caused in waiting for your supplies

department to issue a PO number, a PO number is essential for invoicing and unfortunately we can no

longer reserve places on workshops without this information.






Required Information for booking (cont.) If you are booking more than one place on the same workshop you will need to provide us with a

separate Registration Form for each individual

Individual attending the workshop

Details where invoice is to be sent

(if different from those of the attending

individual)

Name:

Email address:

Department:

Hospital/ Laboratory:

Address:

Postcode:

Tele No:

Email address of the individual booking the

workshop if different from those of the

attending individual:

Contact name:

Email address:

Department:

Hospital/ Laboratory:

Address:

Postcode:

Tele No:

Please note: If a workshop booking is cancelled within one calendar month of the course date

then an administration fee of £50 will be charged.

After completing the form please return to [email protected] for confirmation of

your place on the course. Title your email “CPT Workshop booking”.

For any additional queries please also contact as above.

DO NOT SEND PAYMENT WITH THIS FORM, AN INVOICE WILL BE ISSUED FOLLOWING RECEIPT


Contact Us UK NEQAS for CPT personnel are

happy to help with any bookings or

requests for further information

For more information or to book a place please contact UK NEQAS for CPT:

Susan Mulinda Laura Gamblin

Tel: +44 (0)191 445 6553 Tel: +44 (0)191 445 6553 E: [email protected] E: [email protected]

Providing worldwide external quality assessment and proficiency

testing for all aspects of tissue diagnostics

UK NEQAS for Cellular Pathology Technique |www.ukneqascpt.org.uk |Email: [email protected] |The Pathology

Centre, Queen Elizabeth Hospital, Sheriff Hill, Gateshead, Tyne and Wear NE9 6SX |Tel: +44 (0)191 445 5566

cellular pathology techniques educational workshops brochure 2016...delegates. the workshop is based...

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