cellular respiration
DESCRIPTION
Cellular Respiration. Cellular Respiration = Glucose Oxidation. Redox Reactions. Coenzyme NAD+ is an electron carrier NAD + - oxidized NADH + H + - reduced. NADH - Nicotinamide adenine dinucleotide. Coenzyme found in all cells Made of 2 nucleotides. Mitochondrial structure - label. - PowerPoint PPT PresentationTRANSCRIPT
Cellular Respiration
Cellular Respiration = Glucose Oxidation
Redox Reactions
Coenzyme NAD+ is an electron carrierNAD+ - oxidizedNADH + H+ - reduced
NADH -Nicotinamide adenine dinucleotide
Coenzyme found in all cells
Made of 2 nucleotides
Mitochondrial structure - label
Mitochondrial structure
Cellular Respiration overviewProcess Starting
MoleculeEnd product
Location Substrate level phosphorylation
Energy shuttled to oxidative phosphorylation
Glycolysis 1 glucose 2 pyruvate
Cytosol 2 ATP 2 NADH
(intermediate step)
2 pyruvate
2 Acetyl Co-A, 2 CO2
Matrix of mitochondria
None 2 NADH
Process Starting Molecule
End product
Location Substrate level phosphorylation
Energy shuttled to oxidative phosphorylation
Krebs cycle
2 acetyl-CoA
4 CO2 Matrix of mitochondria
2 ATP 6 NADH,2 FADH2
Oxidative phosphorylation
Electrons (carried by electron carriers)
32-34 ATP(theoretical)
Inner membrane of mitochondria
• Totals entering oxidative phosphorylation:• 4 ATP from substrate phosphorylation• 10 NADH (2 from glycolysis)• 2 FADH2
• NADH from glycolysis 2 ATP (need 1 to shuttle NADH into mitochondria)
• NADH 3 ATP• FADH2 2 ATP • This is theoretical yield
• Total energy produced = 36 – 38 ATP molecules- 2 in glycolysis, 2 in Krebs, 32-34* in
Oxidative phosphorylation• * 34 for plants (don’t spend an ATP to get NADH
into mitochondria), 32 for animals
Glycolysis
• Glyco – glucose Lysis - splitting or breaking
• Pg. 162-163
• How is glucose split?
Glycolysis summary
• How many reactions are required? • What catalyzes each reaction?• How many ATP are produced?• How many net ATP are produced?
• What is the initial reactant?• What are the final products?• Where does this occur in the cell?
Glycolysis
Glycolysis summary
• How many reactions are required? 10• What catalyzes each reaction? Specific enzyme• How many ATP are produced? 4• How many net ATP are produced? 2
• What is the initial reactant? glucose• What are the final products? Pyruvate, 2 ATP, 2
NADH• Where does this occur in the cell? cytosol
Krebs cycle (aka citric acid cycle)
• What is the starting molecules for the Krebs cycle?
• What was the ending molecules of glycolysis?
Krebs cycle (aka citric acid cycle)
• What is the starting molecules for the Krebs cycle? Acetyl CoA
• What was the ending molecules of glycolysis?• pyruvate
Pyruvate to Acetyl CoA• Intermediate step: pyruvate oxidation
• How many reactions needed to convert pyruvate to acetyl CoA?
• What is “lost” in the process?• What is “gained” in the process?
• Where does this occur?
Pyruvate to Acetyl CoA• Intermediate step: pyruvate oxidation
• How many reactions needed to convert pyruvate to acetyl CoA? 3
• What is “lost” in the process? CO2, electron to NAD+• What is “gained” in the process? NADH, Acetyl CoA
• Where does this occur? As pyruvate enters mitochondrion, in the mitochondrial matrix
Krebs Cycle – 1st step
• In first step:• Oxaloacetate (4 C) + Acetyl-CoA (2 C) yields citrate
(6 C)• Oxaloacetate gets regenerated through Krebs cycle
• “-ate” – conjugate bases of the organic acids– Carboxyl groups – can donate protons
• i.e. citrate is the conjugate base of citric acid
Krebs cycle – p. 165• How many reactions?
• What catalyzes these reactions?• How many ATP produced?
• How are the ATP produced?
• Where does the rest of the energy harvested go?
Krebs Cycle
Krebs cycle
• How many reactions? 8 • What catalyzes these reactions? Specific enzymes• How many ATP produced? 1 per cycle (2 total)• How are the ATP produced? Substrate
phosphorylation• Where does the rest of the energy harvested go?• Electron carriers: 3 NADH, 1 FADH2 per cycle
• (6 NADH, 2FADH2 total)
Krebs cycle
• How many turns of the cycle for 1 molecule glucose?
• What are the initial reactants? Final products?
• Where does this occur?
Krebs cycle
• How many turns of the cycle for 1 molecule glucose? 2 – since glucose splits into 2 pyruvate
• What are the initial reactants? Final products?• Initial: 2 Acetyl CoA, 6 NAD+, 2 FAD, 2 ADP• Final: 4 CO2, 6 NADH, 2FADH2, 2 ATP
• Where does this occur? In the mitochondrial matrix
Substrate level phosphorylation
Substrate Level Phosphorylation
• As each bond of glucose is broken, energy is released:– If enough energy released all at once, the energy
is used to directly phosphorylate ADP to make ATP(substrate level phosphorylation)
-- If amount of energy released is small, electrons are taken off as units of energy and handed to an electron shuttle, NADH
NADH gathers all the electrons and passes them off to the electron transport chain , so they can make ATP through oxidative phosphorylation
Oxidative Phosphorylation: Electron Transport Chain & Chemiosmosis
• Electron Transport Chain – see diagram on handout
• Where do the electrons for the electron transport chain come from?
• Why are electrons transferred from carrier to carrier?
• Why does FADH2 enter at a different point than NADH?
Electron Transport Chain/Oxidative Phosphorylation
• Electron Transport Chain – see diagram on handout
• Where do the electrons for the electron transport chain come from? From glycolysis, intermediate, krebs
• Why are electrons transferred from carrier to carrier? Transferred to more electronegative carrier
• Why does FADH2 enter at a different point than NADH? Has higher electronegativity
• What atom is the final acceptor of the electron?
• Why?• What does it form?
• What is gained during this process?
• What atom is the final acceptor of the electron? oxygen
• Why? Most electronegative • What does it form? water
• What is gained during this process? A H+ gradient
Oxidative phosphorylation
• What is the purpose?
• What is a chemiosmotic gradient?
• How does this generate ATP?
Oxidative phosphorylation• What is the purpose? To produce ATP from ADP• What is a chemiosmotic gradient? A difference in
concentration of H+ ions across a membrane (can be used to do work)
• How does this generate ATP? Flow of H+ ions through ATP synthase into mitochondrial matrix cause the ATP synthase to rotate- chemical energy converted to mechanical energy
• This drives phosphorylation of ADP into ATP (ADP + inorganic phosphate)
ATP Synthase
Uses flow of hydrogen ions down gradient to form ATP from inorganic phosphate and ADP
ATP synthase video
• http://www.dnatube.com/video/104/ATP-synthase-structure-and-mechanism
Cellular Respiration
ATP Numbers . . .Not exact –Based on experimental data- 1 molecule glucose yields 29 ATP
NADH – 2.5 ATP, FADH2 – 1.5 ATPNADH from glycolysis in cytosol – electrons get passed to NAD+ or FAD in mitochondrial matrix(which carrier makes a difference in total ATP)Also – some of the proton motive force powers mitochondrion’s uptake of pyruvate from cytosol, also transport of phosphate into mit. matrix
• Cellular respiration efficiency – about 40% of energy from glucose gets stored in ATP
• The rest of the energy is lost as heat
Thermoregulation• Reducing efficiency of cellular respiration• Hibernating mammals – need to maintain body
temperature• Have a channel protein in inner mitochondrial
membrane that allows protons to flow back down concentration gradient without generating ATP
• Allows for oxidation of fats to generate heat without ATP production
• If oxygen is not present, etc and oxidative phosphorylation can’t occur
• 2 ways to produce ATP:• Anaerobic respiration – prokaryotic organisms
in environment without oxygen• Use another final electron acceptor rather than
oxygen, i.e. sulfur• Fermentation
ATP without oxygen
• Makes ATP through glycolysis (only 2 ATP)• NADH transfers its electrons to pyruvate, so
NAD+ can be used again in glycolysis
• Alcoholic fermentation – pyruvate converted to ethyl alcohol and CO2
• Lactic acid fermentation – pyruvate converted to lactate
Fermentation
What about prokaryotes?
• Glycolysis – cytosol• Krebs cycle – cytosol• Electron transport chain – electron carriers in
plasma membrane, gradient gets generated across plasma membrane
• Do not need to transport electrons (in NADH) from glycolysis into mitochondria, so can get more ATP
Evolution & Glycolysis
• Glycolysis is widespread among organisms
• Oldest fossils of bacteria 3.5 billion years old• O2 in atmosphere not until 2.7 billion years
ago
• Perhaps early cells got ATP just through glycolysis
Food Catabolism Proteins, Carbohydrates & Fats can all be used by cellular respiration to make ATP
BiosynthesisIntermediates in the pathway of cell. resp. can be used to synthesize molecules for the cell.
Control of cellular respiration
Phosphofructokinase –Enzyme that catalyzes 3rd step of glycolysis- commitment step for glycolysis
Allosteric enzymeInhibited by ATP, citrateStimulated by AMP