cellulosic ethanol from corn
TRANSCRIPT
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Cellulosic ethanol from wholecorn right here, right now.
Sabrina Trupia, Brian Schoeneck (NCERC), TomLyons (Biotork), Mike Cotta, Bruce Dien, Ron Hector,
Loren Iten (USDA-ARS-NCAUR)
FEW 2013, St.Louis MO
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NCERC Numbers
Established in 2003
Over 17,000 hours of
continuous operation
150+ years of combined
experience in biofuels researchand training
90% self-funded
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Advanced Biofuels Initiative
Enabling NCERC to pursue research projects in the
advanced biofuels space
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Why study corn kernelfiber?
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Other Reasons
Cellulosic material is already at the ethanol plant
The industrial infrastructure is already in place
It increases the yields up to 5-10% per bushel of corn.
It lowers the carbon footprint of existing ethanol plants.
New value-added co-products will become available
It benefits farmers, the existing ethanol industry, the environment,
our energy security
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Been there, done thatCorn Fiber Work at NCERC, largely in collaboration with USDA
2007-2008: Steam activation, characterization
2008-2009: Chemical pretreatment , characterization
2009-2010: Hot water pretreatment, viscosity andenzymatic hydrolysis tests
2011-2012: Corn fiber fermentations (1-150L)
2013: Cellulosic ethanol from corn mash
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The Yeast
Strain
Xylose
consumed
(g/L)Improvement
(%)
D5A 0.9 --YRH400 1.4 56%
YRH400e 1.5 67%
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Fermentation on Xylose Medium
Ethanol + 32 %
Xylose - 11 %
over non-evolved
Xylose conversion efficiency:
41% in 47 hours
Evolved Yeast
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0
1
2
3
4
5
6
0 10 20 30 40 50 60 70 80
OD600
Time (hours)
evolved yeast
non-evolved yeast
Propagation of strains
Final concentration: 28million viable cells/ml
Final concentration: 14
million viable cells/ml
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Corn Fiber Flask Fermentation
D5A: 25.4 g/L
YRH400: 26.5 g/L
YRH400e: 26.6 g/L
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Corn Fiber Scale Up : 30 L
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Scale Up Results: 30 L
15 g/L ethanol produced with
conventional yeast
Ethanol Yield: 0.23 gal/bu ( +8%
additional to starch)
Conversion efficiency: 97% (C6
sugars only, no xylose)
NEXT STEPS:
150 L / evolved yeast
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Scale Up Results: 150 L
Fermentation completed after 48
hours
At end of process, total sugars
down by 65%
100% glucose consumed
72% xylose consumed
NEXT STEP: corn mash
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Cellulosic Ethanol from Corn Mash
Unmodified S. Cerevisiae
Bench-scale tests
72 hours total process time
(48 hour fermentation)
Ethanol concentration: 8%
higher than control
Yield improved by about 14%
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Corn Mash Scale Up(5 L and 30 L)
Evolved modified yeast (YRH400e)
Fermentation completed in 48 hours
At the end of fermentation:
97% of glucose (eq.) consumed
67% xylose consumed
Ethanol concentration: + 10%0
5
10
15
20
25
0.00 20.00 40.00
concentration(%w/v
)
Time (hours)
Glucose Equivalents
Ethanol
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Results Summary
Demonstrated reproducible scale-up: 0.2530150 L
Achieved almost theoretical conversion of sugars in as low as48 hours
Utilized both natural and modified S.Cerevisiae
Corn mash conversion consistent with corn fiber results of
increased ethanol yield
Process under provisional patent
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Acknowledgements
IL Department of Commerce and Economic
Opportunity Illinois Corn Marketing Board
A Specific Cooperative Agreement with the
U.S. Department of Agriculture
Novozymes
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THANK YOU!