Central Dogma (for your disease?)

LB 145

February 22-23, 2011

Deoxyribonucleic Acid

Protein (short protein called peptide)

• Protein =Amino Acids stuck together

• By what kind of bond?

• What kind of reaction is this?

From Gene to Peptide

Functional Peptide: PROTEIN

mRNA

Synthesis ofmRNA in thenucleus

DNA

NUCLEUS

CYTOPLASM

1

mRNA

Synthesis ofmRNA in thenucleus

DNA

NUCLEUS

mRNA

CYTOPLASM

Movement ofmRNA into cytoplasmvia nuclear pore

1

2

mRNA

Synthesis ofmRNA in thenucleus

DNA

NUCLEUS

mRNA

CYTOPLASM

Movement ofmRNA into cytoplasmvia nuclear pore

Ribosome

AminoacidsPolypeptide

Synthesisof protein

1

2

3

Cystic Fibrosis is most often caused by mutation F508 in the CFTR gene

o The CFTR gene is 189,000 bases longThere are 27 exons/ 26 introns in it.o The mRNA (all exons) is 6129 basesHow many codons is that, max?o The protein is 1480 amino acids longNormally it’s a Cl- channel, the mutation?

What do you know of your disease’s Central Dogma?

o What is the size of your gene?How many exons/introns does it have?o What is the size of your mRNA (cDNA)?How many codons does it have?o What is the size of your protein?What is the normal vs abnormal function of it?

Reminders

• Peer Review DUE– Might want to look over the criticisms soon-ish

• Order your primers by 5pm FRIDAY

• Check ATCC.org for cells and DNA.