Luminescent Immunoassays (LIA, ILMA)By replacing colorimetric substrates of horse-radish peroxidase or phosphatases with lumi-nescent ones an increase in sensitivity up to 100-fold can be achieved. MikroWin software withthe curve fitting option adds convenient andextensive data evaluation capabilities to thesuperb instrument performance.

Even the fastestflash-type Acri-dinium esterbased immuno-assays can bemeasured withthe Centro.

DNA probe assaysSeveral diagnostic DNA probe assays based onacridinium ester labelled oligo-nucleotides arecommonly used providing the most sensitivedetection and diagnosis of infectious diseases.

SNP determinationSNPs are the most common type of human genetic variability playing a key role in the diversityof development of diseases and response to therapeutic treatments.

CALUX® AssaysThe CALUX® assays use genetically modifiedmammalian cell lines that contains the firefly luci-ferase gene as a reporter for certain chemicals asdioxin, dioxin-like PCBs, (anti-)estrogen or (anti-)androgen compounds.

The CALUX® cellshave been tailo-red so that theyproduce selec-tively the light ina dose responsiveway when expo-sed to the chemi-cals.

Applications

Reporter Gene AssaysIn basic research of generegulation as well as in drug discovery the use ofluciferases, ß-galactosidases,ß-glucuronidases and secreted alkaline phosphatases have become a standard tool offering the highest sensitivity.

Especially the dual luminescence type assays,e.g. Dual-Luciferase® Reporter Assay, have become a favourite means as they provide aninternal control for transfection efficiency orgeneral expression level.

Aequorin based Calcium monitoring

The monitoring of the changes in intracellular Ca++

levels by the means of aequorin in a flash-typeluminescent reaction is highly efficient. Assays forboth, the activation of G-protein coupled receptors(GPCRs or 7TM receptors) inducing inositol phospholipid degradation and the activity of voltage gated Ca++ ion channels – the latter causing a rather slow influx – can easily be performed with the use of the Centro´s reagentinjectors.

ATP determinationA detection limit of less than 1.5 attomoles of ATPper well makes the Centro XS3 one of the best suitedmicroplate instruments for the determination ofcell viability, e.g. in tumour chemosensitivityassays, cell proliferation, antibiotic susceptibilitytesting or bacterial detection.

Centro / Centro XS3 LB 960Technical Specification and Order Information

detect and identify

CentroCentro XS3

LB 960Microplate Luminometers

BERTHOLD TECHNOLOGIES reserves the right to implement technicalimprovements and/or design changes without prior notice. Dual-Luciferase, DLR, DLReady and Dual-Glo are trademarks of PromegaCorporation. Windows is a registered trademark of Microsoft. CALUX® is a trademark of BioDetection Systems.

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Detection unit Low–noise photomultiplier tubein single photon counting modeSpectral range: 340-630 nm

Sensitivity < 1.5 amol ATP (Centro XS3)< 1.5 zmol firefly lucif. (Centro XS3)< 5 amol ATP (Centro)< 2 zmol firefly luciferase (Centro)

Dynamic range > 6 orders of magnitudeCrosstalk Low crosstalk through crosstalk

reduction design: 10-6

Injection unit Up to 3 injectors variable volume: 10-100 µL, JET Injection technology

Plate formats All 96 and 384 well microplateswith outer dimensions:86.0 x 128.2 x 14.7 mm (WxLxH)

Robotic integration OptionalInterface Serial RS232, 9 pinPC operating system Win 2000, Win NT, Win XP, Win VistaPC requirements Pentium processor, 500 MHz

(or better), CD ROM drive, display1024 x 768 (or better), serial port

Software MikroWin 2000Power supply 110 – 240 V; 50/60 Hz; 70 VARegulations CE, UL, CSATemperature range Storage: 0 ° – 40 °C

Operation: 15 ° – 35 °CHumidity 10 – 85 % non condensingDimensions 330 x 430 x 295 mm (WxDxH)Weight 17 kg

Operation modesIntegral 0.1 - 600 secKinetics total time up to 24 hRepeated total time up to 24 hPlate repeats up to 999Dispensing 3 independent variable volume

injectorsShaking 3 modes, variable amplitude and

speedDelay up to 3600 sec

Order Information Order Number Centro LB 960LB 960 Centro Basic 38100-1096 well – upgradeable, MikroWin Lite*

LB 960 Centro Research 38100-5096 well, 2 reagent injectors,waste pump, MikroWin Lite*

LB 960 Centro HTS 38100-5196 & 384 well, 3 reagent injectors, waste pump, robot integration module, MikroWin Adv. II*

LB 960 Centro Phago 38100-5296 well, 3 reagent injectors, waste pump, temperature control, MikroWin Lite*

Options for CentroInjector 1 pre-position 2, 10 - 100 µL 37772-01Injector 2 pre-position 1, 10 - 100 µL 37772-02Injector 3 meas. position, 10 - 100 µL 37772-03384 well upgrade 38211Temperature control 96 well version 41064Temperature control 96/384 well version 41063Centro XS3 LB 960Centro XS3 Basic 46970-10enhanced sensitivity, 96/384 well, upgradeable with injectors, MikroWin Lite*

Centro XS3 Reporter gene 46970-50enhanced sensitivity, 96/384 well, 2 reagent injectors, MikroWin Lite*

Options for Centro XS3

Injector assembly with 3 injectors 48532(#3, #2, #1), 10 - 100 µL eachInjector assembly with 2 injectors 48533(#3, #2), 10 - 100 µL eachInjector assembly with 1 injector 48775(#3), 10 - 100 µLInjector 1 pre-position 3, 10 - 100 µL 37772-21Injector 2 pre-position 2, 10 - 100 µL 37772-22Options & Accessories for all Centro modelsWaste pump 38216Robot integration module 39368Luminescence testplate for QC 40105-10Luminescence test kit for QC 45366Cleanit Daily, injector cleaning solution 45218Microplates 96 well, white, 100 pieces 23300Microplates 96 well, white, sterile 50 pieces 51838Microplates, 96 well, white, 24910 clearbottom, cell culture, 100 piecesMicroplates 384 well, white, 40 pieces 32505*Upgrade (MikroWin Advanced versions I or II) andadditional licences available

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: Bio@Berthold.comInternet: www.Berthold.com/Bio

Default parameter files, e.g. Dual-Luciferase®, and Dual-GloTM reportergene, Kinetics and Repeated type (Cellular Luminescence), etc.

Patents: EP 1 279 948 A 1, DE 101 36 866 A 1 (pending)

Accessories

QC luminescence test kitWith the QC luminescence test kit the performanceof your luminometer can be checked. This is theideal quality control (QC) methodas performance of both injectionand detection systems can bemonitored. The test kit follows afast flash–type kinetics. The test kitcontains a „label” and two types of starterreagents.

Injector Cleaning SolutionFor proper maintenance of the injection system the Cleanit solutionsare recommended for regularcleaning. Daily cleaning ensures thataccuracy and precision as well aslong life cycle of the injectors will bemaintained.

QC testplate for luminescenceWith the luminescence testplate you can check easily perfor-mance of your Centro luminometer.Selection of 96 or 384 well formatand overload signal is possible.

MicroplatesBERTHOLD TECHNOLOGIES’microplates for luminescenceapplications will provide you bestquality, low background and lowestcrosstalk. They are available indifferent plate formats andoptionally with clear bottom.

Centro / Centro XS3 LB 960Microplate Luminometer

Plate formatsAny opaque microplate in either 96 or 384 well format can be measured. There is noneed for any mechanical adjustment – just select the plate type in the operating software.

Temperature controlThe Centro can be equipped with plate heating forapplications where the assay must be measured atelevated and constant temperatures as common incell based assays.

The sandwich-type heating plate design reducesevaporation effects and ensures homo-geneous temperature distribution.

Robot accessSpecially designed for, but not restricted to, use inHTS departments of drug discovery companies, the Centro as well as the Centro XS3 robot accessmodule allows for easy integration of the unit intoany type of lab automation system.

Software and hardware easily interface into existing robot, stacker or liquid handling systems. A barcode reader may be used for positive plateidentification.

ShakingWith three modes, eachwith variable speeds toselect, the integrated shaker enhances mixing and optimisessample distribution before measu-rements especially in long termkinetics.

Small footprintThe ergonomic design of the LB 960 Centro models requires minimal bench space. All the importantfeatures are built in optimising the use of available bench space.

The reagent injectors are housed within the smallfootprint, providing the advantage of short tubingand thus a low dead volume.

Software

MikroWin 2000The Windows® based software combines operationand definition of instrument settings as well as datareduction and evaluation.

The wells for measurement and injection can beselected independently. Various operations can beselected and placed in any order to accommodatethe different requirements of the assay.

Kinetic data reduction and graphical display of the respective curves helps the user to analyse the results. Any type of ratio calculation, e.g. DLR,or other mathematical formulas can be linked toeach individual well.

Standard curve fitting and calculation of unknownsamples is available for those users looking forquantification of their results.

All data can be exported in EXCEL® or ASCII formats.

Reagent dispensing3 independently controlled injectors with variablevolume give entire freedom in the selection ofassay type and assay sequence.

The volume is adjustable from 10 to 100 µL– perfectly fitting the demands for reagent addition into 96 and 384 well plates.

In Centro dispensing can even be performedduring a kinetic measurement, e.g. to watch theeffects of added agonists / antagonists.

JET injection technology stands for precision andaccuracy – better than 99 % and 98 % respectively – and the only reliable way of properly mixing theadded reagents.

Centro & Centro XS3

ATP performance

10000000

1000000

100000

10000

1000

100

10

3 50 780

1250

0

cou

nts

ATP (amol/well)

Centro XS3

detection limit< 1.5 amol ATP / well

R2 = 0.9963

R2 = 0.9974Centrodetection limit< 5 amol ATP / well

detect and identify

The Centro and Centro XS3 meet all currently knownrequirements for versatile, robust and sensitivemicroplate luminometers. The selected detectors and the proprietary design of the optical systemguarantee lowest background and negligible cross-talk. Centro XS3 has an optimized optics for evenhigher demands on sensitivity.

DLReadyTM

Fulfilling and even exceedingthe requirements to qualify asDLReadyTM, the Centro XS3 LB 960 is the ideal instru-ment for luciferase reporter gene assays especiallyPromega´s Dual-Luciferase® reporter gene assaysystem.

High precision and efficiency of the injectors provide the opportunity to perform the assays rapidly – making it more applicable for screening environments.

BERTHOLD TECHNOLOGIES offers 4 different, pre-defined application oriented Centro models:

Centro Researchmeets the common needs for a versatile lumino-meter in general research incl. reporter geneassays, enzyme studies, etc.

Centro HTSequipped with all options required in screeningenvironments, e.g. injectors, 384 well readingand robot integration module

Centro Phagoequipped with temperature control and injectorsto perform cellular luminescence applicationslike Phagocytosis monitoring

Centro XS3 Reporter Genespecifically configured enabling reporter gene appli-cations like the Dual-Luciferase® reporter geneassay with utmost sensitivity due to the optimizedoptics

Additionally there are two flexible Centro models thatallow upgrades with different options according toyour demands:

Centro Basicall luminescence applications and possibility toadd the following options: 384 well format,up to 3 injectors, temperature control

Centro XS3 Basicall luminescent applications with enhanced sensitivity and the possibility to add up to 3 injectors

Accessories

QC luminescence test kitWith the QC luminescence test kit the performanceof your luminometer can be checked. This is theideal quality control (QC) methodas performance of both injectionand detection systems can bemonitored. The test kit follows afast flash–type kinetics. The test kitcontains a „label” and two types of starterreagents.

Injector Cleaning SolutionFor proper maintenance of the injection system the Cleanit solutionsare recommended for regularcleaning. Daily cleaning ensures thataccuracy and precision as well aslong life cycle of the injectors will bemaintained.

QC testplate for luminescenceWith the luminescence testplate you can check easily perfor-mance of your Centro luminometer.Selection of 96 or 384 well formatand overload signal is possible.

MicroplatesBERTHOLD TECHNOLOGIES’microplates for luminescenceapplications will provide you bestquality, low background and lowestcrosstalk. They are available indifferent plate formats andoptionally with clear bottom.

Centro / Centro XS3 LB 960Microplate Luminometer

Plate formatsAny opaque microplate in either 96 or 384 well format can be measured. There is noneed for any mechanical adjustment – just select the plate type in the operating software.

Temperature controlThe Centro can be equipped with plate heating forapplications where the assay must be measured atelevated and constant temperatures as common incell based assays.

The sandwich-type heating plate design reducesevaporation effects and ensures homo-geneous temperature distribution.

Robot accessSpecially designed for, but not restricted to, use inHTS departments of drug discovery companies, the Centro as well as the Centro XS3 robot accessmodule allows for easy integration of the unit intoany type of lab automation system.

Software and hardware easily interface into existing robot, stacker or liquid handling systems. A barcode reader may be used for positive plateidentification.

ShakingWith three modes, eachwith variable speeds toselect, the integrated shaker enhances mixing and optimisessample distribution before measu-rements especially in long termkinetics.

Small footprintThe ergonomic design of the LB 960 Centro models requires minimal bench space. All the importantfeatures are built in optimising the use of available bench space.

The reagent injectors are housed within the smallfootprint, providing the advantage of short tubingand thus a low dead volume.

Software

MikroWin 2000The Windows® based software combines operationand definition of instrument settings as well as datareduction and evaluation.

The wells for measurement and injection can beselected independently. Various operations can beselected and placed in any order to accommodatethe different requirements of the assay.

Kinetic data reduction and graphical display of the respective curves helps the user to analyse the results. Any type of ratio calculation, e.g. DLR,or other mathematical formulas can be linked toeach individual well.

Standard curve fitting and calculation of unknownsamples is available for those users looking forquantification of their results.

All data can be exported in EXCEL® or ASCII formats.

Reagent dispensing3 independently controlled injectors with variablevolume give entire freedom in the selection ofassay type and assay sequence.

The volume is adjustable from 10 to 100 µL– perfectly fitting the demands for reagent addition into 96 and 384 well plates.

In Centro dispensing can even be performedduring a kinetic measurement, e.g. to watch theeffects of added agonists / antagonists.

JET injection technology stands for precision andaccuracy – better than 99 % and 98 % respectively – and the only reliable way of properly mixing theadded reagents.

Centro & Centro XS3

ATP performance

10000000

1000000

100000

10000

1000

100

10

3 50 780

1250

0

cou

nts

ATP (amol/well)

Centro XS3

detection limit< 1.5 amol ATP / well

R2 = 0.9963

R2 = 0.9974Centrodetection limit< 5 amol ATP / well

detect and identify

The Centro and Centro XS3 meet all currently knownrequirements for versatile, robust and sensitivemicroplate luminometers. The selected detectors and the proprietary design of the optical systemguarantee lowest background and negligible cross-talk. Centro XS3 has an optimized optics for evenhigher demands on sensitivity.

DLReadyTM

Fulfilling and even exceedingthe requirements to qualify asDLReadyTM, the Centro XS3 LB 960 is the ideal instru-ment for luciferase reporter gene assays especiallyPromega´s Dual-Luciferase® reporter gene assaysystem.

High precision and efficiency of the injectors provide the opportunity to perform the assays rapidly – making it more applicable for screening environments.

BERTHOLD TECHNOLOGIES offers 4 different, pre-defined application oriented Centro models:

Centro Researchmeets the common needs for a versatile lumino-meter in general research incl. reporter geneassays, enzyme studies, etc.

Centro HTSequipped with all options required in screeningenvironments, e.g. injectors, 384 well readingand robot integration module

Centro Phagoequipped with temperature control and injectorsto perform cellular luminescence applicationslike Phagocytosis monitoring

Centro XS3 Reporter Genespecifically configured enabling reporter gene appli-cations like the Dual-Luciferase® reporter geneassay with utmost sensitivity due to the optimizedoptics

Additionally there are two flexible Centro models thatallow upgrades with different options according toyour demands:

Centro Basicall luminescence applications and possibility toadd the following options: 384 well format,up to 3 injectors, temperature control

Centro XS3 Basicall luminescent applications with enhanced sensitivity and the possibility to add up to 3 injectors

Accessories

QC luminescence test kitWith the QC luminescence test kit the performanceof your luminometer can be checked. This is theideal quality control (QC) methodas performance of both injectionand detection systems can bemonitored. The test kit follows afast flash–type kinetics. The test kitcontains a „label” and two types of starterreagents.

Injector Cleaning SolutionFor proper maintenance of the injection system the Cleanit solutionsare recommended for regularcleaning. Daily cleaning ensures thataccuracy and precision as well aslong life cycle of the injectors will bemaintained.

QC testplate for luminescenceWith the luminescence testplate you can check easily perfor-mance of your Centro luminometer.Selection of 96 or 384 well formatand overload signal is possible.

MicroplatesBERTHOLD TECHNOLOGIES’microplates for luminescenceapplications will provide you bestquality, low background and lowestcrosstalk. They are available indifferent plate formats andoptionally with clear bottom.

Centro / Centro XS3 LB 960Microplate Luminometer

Plate formatsAny opaque microplate in either 96 or 384 well format can be measured. There is noneed for any mechanical adjustment – just select the plate type in the operating software.

Temperature controlThe Centro can be equipped with plate heating forapplications where the assay must be measured atelevated and constant temperatures as common incell based assays.

The sandwich-type heating plate design reducesevaporation effects and ensures homo-geneous temperature distribution.

Robot accessSpecially designed for, but not restricted to, use inHTS departments of drug discovery companies, the Centro as well as the Centro XS3 robot accessmodule allows for easy integration of the unit intoany type of lab automation system.

Software and hardware easily interface into existing robot, stacker or liquid handling systems. A barcode reader may be used for positive plateidentification.

ShakingWith three modes, eachwith variable speeds toselect, the integrated shaker enhances mixing and optimisessample distribution before measu-rements especially in long termkinetics.

Small footprintThe ergonomic design of the LB 960 Centro models requires minimal bench space. All the importantfeatures are built in optimising the use of available bench space.

The reagent injectors are housed within the smallfootprint, providing the advantage of short tubingand thus a low dead volume.

Software

MikroWin 2000The Windows® based software combines operationand definition of instrument settings as well as datareduction and evaluation.

The wells for measurement and injection can beselected independently. Various operations can beselected and placed in any order to accommodatethe different requirements of the assay.

Kinetic data reduction and graphical display of the respective curves helps the user to analyse the results. Any type of ratio calculation, e.g. DLR,or other mathematical formulas can be linked toeach individual well.

Standard curve fitting and calculation of unknownsamples is available for those users looking forquantification of their results.

All data can be exported in EXCEL® or ASCII formats.

Reagent dispensing3 independently controlled injectors with variablevolume give entire freedom in the selection ofassay type and assay sequence.

The volume is adjustable from 10 to 100 µL– perfectly fitting the demands for reagent addition into 96 and 384 well plates.

In Centro dispensing can even be performedduring a kinetic measurement, e.g. to watch theeffects of added agonists / antagonists.

JET injection technology stands for precision andaccuracy – better than 99 % and 98 % respectively – and the only reliable way of properly mixing theadded reagents.

Centro & Centro XS3

ATP performance

10000000

1000000

100000

10000

1000

100

10

3 50 780

1250

0

cou

nts

ATP (amol/well)

Centro XS3

detection limit< 1.5 amol ATP / well

R2 = 0.9963

R2 = 0.9974Centrodetection limit< 5 amol ATP / well

detect and identify

The Centro and Centro XS3 meet all currently knownrequirements for versatile, robust and sensitivemicroplate luminometers. The selected detectors and the proprietary design of the optical systemguarantee lowest background and negligible cross-talk. Centro XS3 has an optimized optics for evenhigher demands on sensitivity.

DLReadyTM

Fulfilling and even exceedingthe requirements to qualify asDLReadyTM, the Centro XS3 LB 960 is the ideal instru-ment for luciferase reporter gene assays especiallyPromega´s Dual-Luciferase® reporter gene assaysystem.

High precision and efficiency of the injectors provide the opportunity to perform the assays rapidly – making it more applicable for screening environments.

BERTHOLD TECHNOLOGIES offers 4 different, pre-defined application oriented Centro models:

Centro Researchmeets the common needs for a versatile lumino-meter in general research incl. reporter geneassays, enzyme studies, etc.

Centro HTSequipped with all options required in screeningenvironments, e.g. injectors, 384 well readingand robot integration module

Centro Phagoequipped with temperature control and injectorsto perform cellular luminescence applicationslike Phagocytosis monitoring

Centro XS3 Reporter Genespecifically configured enabling reporter gene appli-cations like the Dual-Luciferase® reporter geneassay with utmost sensitivity due to the optimizedoptics

Additionally there are two flexible Centro models thatallow upgrades with different options according toyour demands:

Centro Basicall luminescence applications and possibility toadd the following options: 384 well format,up to 3 injectors, temperature control

Centro XS3 Basicall luminescent applications with enhanced sensitivity and the possibility to add up to 3 injectors

Luminescent Immunoassays (LIA, ILMA)By replacing colorimetric substrates of horse-radish peroxidase or phosphatases with lumi-nescent ones an increase in sensitivity up to 100-fold can be achieved. MikroWin software withthe curve fitting option adds convenient andextensive data evaluation capabilities to thesuperb instrument performance.

Even the fastestflash-type Acri-dinium esterbased immuno-assays can bemeasured withthe Centro.

DNA probe assaysSeveral diagnostic DNA probe assays based onacridinium ester labelled oligo-nucleotides arecommonly used providing the most sensitivedetection and diagnosis of infectious diseases.

SNP determinationSNPs are the most common type of human genetic variability playing a key role in the diversityof development of diseases and response to therapeutic treatments.

CALUX® AssaysThe CALUX® assays use genetically modifiedmammalian cell lines that contains the firefly luci-ferase gene as a reporter for certain chemicals asdioxin, dioxin-like PCBs, (anti-)estrogen or (anti-)androgen compounds.

The CALUX® cellshave been tailo-red so that theyproduce selec-tively the light ina dose responsiveway when expo-sed to the chemi-cals.

Applications

Reporter Gene AssaysIn basic research of generegulation as well as in drug discovery the use ofluciferases, ß-galactosidases,ß-glucuronidases and secreted alkaline phosphatases have become a standard tool offering the highest sensitivity.

Especially the dual luminescence type assays,e.g. Dual-Luciferase® Reporter Assay, have become a favourite means as they provide aninternal control for transfection efficiency orgeneral expression level.

Aequorin based Calcium monitoring

The monitoring of the changes in intracellular Ca++

levels by the means of aequorin in a flash-typeluminescent reaction is highly efficient. Assays forboth, the activation of G-protein coupled receptors(GPCRs or 7TM receptors) inducing inositol phospholipid degradation and the activity of voltage gated Ca++ ion channels – the latter causing a rather slow influx – can easily be performed with the use of the Centro´s reagentinjectors.

ATP determinationA detection limit of less than 1.5 attomoles of ATPper well makes the Centro XS3 one of the best suitedmicroplate instruments for the determination ofcell viability, e.g. in tumour chemosensitivityassays, cell proliferation, antibiotic susceptibilitytesting or bacterial detection.

Centro / Centro XS3 LB 960Technical Specification and Order Information

detect and identify

CentroCentro XS3

LB 960Microplate Luminometers

BERTHOLD TECHNOLOGIES reserves the right to implement technicalimprovements and/or design changes without prior notice. Dual-Luciferase, DLR, DLReady and Dual-Glo are trademarks of PromegaCorporation. Windows is a registered trademark of Microsoft. CALUX® is a trademark of BioDetection Systems.

Cen

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· 1

0-20

08·3

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· Id

.Nr.:

381

00PR

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ev03

Detection unit Low–noise photomultiplier tubein single photon counting modeSpectral range: 340-630 nm

Sensitivity < 1.5 amol ATP (Centro XS3)< 1.5 zmol firefly lucif. (Centro XS3)< 5 amol ATP (Centro)< 2 zmol firefly luciferase (Centro)

Dynamic range > 6 orders of magnitudeCrosstalk Low crosstalk through crosstalk

reduction design: 10-6

Injection unit Up to 3 injectors variable volume: 10-100 µL, JET Injection technology

Plate formats All 96 and 384 well microplateswith outer dimensions:86.0 x 128.2 x 14.7 mm (WxLxH)

Robotic integration OptionalInterface Serial RS232, 9 pinPC operating system Win 2000, Win NT, Win XP, Win VistaPC requirements Pentium processor, 500 MHz

(or better), CD ROM drive, display1024 x 768 (or better), serial port

Software MikroWin 2000Power supply 110 – 240 V; 50/60 Hz; 70 VARegulations CE, UL, CSATemperature range Storage: 0 ° – 40 °C

Operation: 15 ° – 35 °CHumidity 10 – 85 % non condensingDimensions 330 x 430 x 295 mm (WxDxH)Weight 17 kg

Operation modesIntegral 0.1 - 600 secKinetics total time up to 24 hRepeated total time up to 24 hPlate repeats up to 999Dispensing 3 independent variable volume

injectorsShaking 3 modes, variable amplitude and

speedDelay up to 3600 sec

Order Information Order Number Centro LB 960LB 960 Centro Basic 38100-1096 well – upgradeable, MikroWin Lite*

LB 960 Centro Research 38100-5096 well, 2 reagent injectors,waste pump, MikroWin Lite*

LB 960 Centro HTS 38100-5196 & 384 well, 3 reagent injectors, waste pump, robot integration module, MikroWin Adv. II*

LB 960 Centro Phago 38100-5296 well, 3 reagent injectors, waste pump, temperature control, MikroWin Lite*

Options for CentroInjector 1 pre-position 2, 10 - 100 µL 37772-01Injector 2 pre-position 1, 10 - 100 µL 37772-02Injector 3 meas. position, 10 - 100 µL 37772-03384 well upgrade 38211Temperature control 96 well version 41064Temperature control 96/384 well version 41063Centro XS3 LB 960Centro XS3 Basic 46970-10enhanced sensitivity, 96/384 well, upgradeable with injectors, MikroWin Lite*

Centro XS3 Reporter gene 46970-50enhanced sensitivity, 96/384 well, 2 reagent injectors, MikroWin Lite*

Options for Centro XS3

Injector assembly with 3 injectors 48532(#3, #2, #1), 10 - 100 µL eachInjector assembly with 2 injectors 48533(#3, #2), 10 - 100 µL eachInjector assembly with 1 injector 48775(#3), 10 - 100 µLInjector 1 pre-position 3, 10 - 100 µL 37772-21Injector 2 pre-position 2, 10 - 100 µL 37772-22Options & Accessories for all Centro modelsWaste pump 38216Robot integration module 39368Luminescence testplate for QC 40105-10Luminescence test kit for QC 45366Cleanit Daily, injector cleaning solution 45218Microplates 96 well, white, 100 pieces 23300Microplates 96 well, white, sterile 50 pieces 51838Microplates, 96 well, white, 24910 clearbottom, cell culture, 100 piecesMicroplates 384 well, white, 40 pieces 32505*Upgrade (MikroWin Advanced versions I or II) andadditional licences available

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: Bio@Berthold.comInternet: www.Berthold.com/Bio

Default parameter files, e.g. Dual-Luciferase®, and Dual-GloTM reportergene, Kinetics and Repeated type (Cellular Luminescence), etc.

Patents: EP 1 279 948 A 1, DE 101 36 866 A 1 (pending)

Luminescent Immunoassays (LIA, ILMA)By replacing colorimetric substrates of horse-radish peroxidase or phosphatases with lumi-nescent ones an increase in sensitivity up to 100-fold can be achieved. MikroWin software withthe curve fitting option adds convenient andextensive data evaluation capabilities to thesuperb instrument performance.

Even the fastestflash-type Acri-dinium esterbased immuno-assays can bemeasured withthe Centro.

DNA probe assaysSeveral diagnostic DNA probe assays based onacridinium ester labelled oligo-nucleotides arecommonly used providing the most sensitivedetection and diagnosis of infectious diseases.

SNP determinationSNPs are the most common type of human genetic variability playing a key role in the diversityof development of diseases and response to therapeutic treatments.

CALUX® AssaysThe CALUX® assays use genetically modifiedmammalian cell lines that contains the firefly luci-ferase gene as a reporter for certain chemicals asdioxin, dioxin-like PCBs, (anti-)estrogen or (anti-)androgen compounds.

The CALUX® cellshave been tailo-red so that theyproduce selec-tively the light ina dose responsiveway when expo-sed to the chemi-cals.

Applications

Reporter Gene AssaysIn basic research of generegulation as well as in drug discovery the use ofluciferases, ß-galactosidases,ß-glucuronidases and secreted alkaline phosphatases have become a standard tool offering the highest sensitivity.

Especially the dual luminescence type assays,e.g. Dual-Luciferase® Reporter Assay, have become a favourite means as they provide aninternal control for transfection efficiency orgeneral expression level.

Aequorin based Calcium monitoring

The monitoring of the changes in intracellular Ca++

levels by the means of aequorin in a flash-typeluminescent reaction is highly efficient. Assays forboth, the activation of G-protein coupled receptors(GPCRs or 7TM receptors) inducing inositol phospholipid degradation and the activity of voltage gated Ca++ ion channels – the latter causing a rather slow influx – can easily be performed with the use of the Centro´s reagentinjectors.

ATP determinationA detection limit of less than 1.5 attomoles of ATPper well makes the Centro XS3 one of the best suitedmicroplate instruments for the determination ofcell viability, e.g. in tumour chemosensitivityassays, cell proliferation, antibiotic susceptibilitytesting or bacterial detection.

Centro / Centro XS3 LB 960Technical Specification and Order Information

detect and identify

CentroCentro XS3

LB 960Microplate Luminometers

BERTHOLD TECHNOLOGIES reserves the right to implement technicalimprovements and/or design changes without prior notice. Dual-Luciferase, DLR, DLReady and Dual-Glo are trademarks of PromegaCorporation. Windows is a registered trademark of Microsoft. CALUX® is a trademark of BioDetection Systems.

Cen

tro

· 1

0-20

08·3

000

· Id

.Nr.:

381

00PR

2 R

ev03

Detection unit Low–noise photomultiplier tubein single photon counting modeSpectral range: 340-630 nm

Sensitivity < 1.5 amol ATP (Centro XS3)< 1.5 zmol firefly lucif. (Centro XS3)< 5 amol ATP (Centro)< 2 zmol firefly luciferase (Centro)

Dynamic range > 6 orders of magnitudeCrosstalk Low crosstalk through crosstalk

reduction design: 10-6

Injection unit Up to 3 injectors variable volume: 10-100 µL, JET Injection technology

Plate formats All 96 and 384 well microplateswith outer dimensions:86.0 x 128.2 x 14.7 mm (WxLxH)

Robotic integration OptionalInterface Serial RS232, 9 pinPC operating system Win 2000, Win NT, Win XP, Win VistaPC requirements Pentium processor, 500 MHz

(or better), CD ROM drive, display1024 x 768 (or better), serial port

Software MikroWin 2000Power supply 110 – 240 V; 50/60 Hz; 70 VARegulations CE, UL, CSATemperature range Storage: 0 ° – 40 °C

Operation: 15 ° – 35 °CHumidity 10 – 85 % non condensingDimensions 330 x 430 x 295 mm (WxDxH)Weight 17 kg

Operation modesIntegral 0.1 - 600 secKinetics total time up to 24 hRepeated total time up to 24 hPlate repeats up to 999Dispensing 3 independent variable volume

injectorsShaking 3 modes, variable amplitude and

speedDelay up to 3600 sec

Order Information Order Number Centro LB 960LB 960 Centro Basic 38100-1096 well – upgradeable, MikroWin Lite*

LB 960 Centro Research 38100-5096 well, 2 reagent injectors,waste pump, MikroWin Lite*

LB 960 Centro HTS 38100-5196 & 384 well, 3 reagent injectors, waste pump, robot integration module, MikroWin Adv. II*

LB 960 Centro Phago 38100-5296 well, 3 reagent injectors, waste pump, temperature control, MikroWin Lite*

Options for CentroInjector 1 pre-position 2, 10 - 100 µL 37772-01Injector 2 pre-position 1, 10 - 100 µL 37772-02Injector 3 meas. position, 10 - 100 µL 37772-03384 well upgrade 38211Temperature control 96 well version 41064Temperature control 96/384 well version 41063Centro XS3 LB 960Centro XS3 Basic 46970-10enhanced sensitivity, 96/384 well, upgradeable with injectors, MikroWin Lite*

Centro XS3 Reporter gene 46970-50enhanced sensitivity, 96/384 well, 2 reagent injectors, MikroWin Lite*

Options for Centro XS3

Injector assembly with 3 injectors 48532(#3, #2, #1), 10 - 100 µL eachInjector assembly with 2 injectors 48533(#3, #2), 10 - 100 µL eachInjector assembly with 1 injector 48775(#3), 10 - 100 µLInjector 1 pre-position 3, 10 - 100 µL 37772-21Injector 2 pre-position 2, 10 - 100 µL 37772-22Options & Accessories for all Centro modelsWaste pump 38216Robot integration module 39368Luminescence testplate for QC 40105-10Luminescence test kit for QC 45366Cleanit Daily, injector cleaning solution 45218Microplates 96 well, white, 100 pieces 23300Microplates 96 well, white, sterile 50 pieces 51838Microplates, 96 well, white, 24910 clearbottom, cell culture, 100 piecesMicroplates 384 well, white, 40 pieces 32505*Upgrade (MikroWin Advanced versions I or II) andadditional licences available

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: Bio@Berthold.comInternet: www.Berthold.com/Bio

Default parameter files, e.g. Dual-Luciferase®, and Dual-GloTM reportergene, Kinetics and Repeated type (Cellular Luminescence), etc.

Patents: EP 1 279 948 A 1, DE 101 36 866 A 1 (pending)

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