c:\fakepath\ start here ch01 lecture
DESCRIPTION
Microbiology Chapter 1TRANSCRIPT
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
Lecture prepared by Mindy Miller-Kittrell, University of Tennessee, Knoxville
M I C R O B I O L O G YWITH DISEASES BY BODY SYSTEM SECOND EDITION
Chapter 1 A Brief History of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• What Does Life Really Look Like?– Antoni van Leeuwenhoek (Dutch)
– Began making and using simple microscopes– Often made a new microscope for each specimen– Examined water and visualized tiny animals, fungi, algae,
and single-celled protozoa; “animalcules”
– By end of 19th century, these organisms were called microorganisms
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• How Can Microbes Be Classified?– Carolus Linnaeus (Swedish) developed taxonomic system for
naming plants and animals and grouping similar organisms together
– Leeuwenhoek’s microorganisms grouped into six categories as follows:
– Fungi– Protozoa– Algae– Bacteria– Archaea– Small animals
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• Fungi– Eukaryotic (have membrane-bound nucleus)– Obtain food from other organisms– Possess cell walls– Composed of
– Molds – multicellular; have hyphae; reproduce by sexual and asexual spores
– Yeasts – unicellular; reproduce asexually by budding; some produce sexual spores
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• Protozoa– Single-celled eukaryotes– Similar to animals in nutrient needs and cellular structure– Live freely in water; some live in animal hosts– Asexual (most) and sexual reproduction– Most are capable of locomotion by
– Pseudopodia – cell extensions that flow in direction of travel– Cilia – numerous, short, hairlike protrusions that propel
organisms through environment– Flagella – extensions of a cell that are fewer, longer, and
more whiplike than cilia
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• Algae
– Unicellular or multicellular– Photosynthetic– Simple reproductive structures– Categorized on the basis of pigmentation, storage products, and
composition of cell wall
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
• Bacteria and Archaea– Unicellular and lack nuclei– Much smaller than eukaryotes– Found everywhere there is sufficient moisture; some found in
extreme environments– Reproduce asexually– Two kinds
– Bacteria – cell walls contain peptidoglycan; some lack cell walls; most do not cause disease and some are beneficial
– Archaea – cell walls composed of polymers other than peptidoglycan
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Early Years of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Scientists searched for answers to four questions– Is spontaneous generation of microbial life possible?– What causes fermentation?– What causes disease?– How can we prevent infection and disease?
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Some philosophers and scientists of the past thought living things arose from three processes: asexual reproduction, sexual reproduction, or from nonliving matter
• Aristotle proposed spontaneous generation – living things can arise from nonliving matter
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Redi’s Experiments
– When decaying meat was kept isolated from flies, maggots never developed
– Meat exposed to flies was soon infested– As a result, scientists began to doubt Aristotle’s theory
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Needham’s Experiments
– Scientists did not believe animals could arise spontaneously, but did believe microbes could
– Needham’s experiments with beef gravy and infusions of plant material reinforced this idea
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Spallanzani’s Experiments– Concluded that
– Needham failed to heat vials sufficiently to kill all microbes or had not sealed vials tightly enough
– Microorganisms exist in air and can contaminate experiments– Spontaneous generation of microorganisms does not occur
– Critics said sealed vials did not allow enough air for organisms to survive and that prolonged heating destroyed “life force”
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Pasteur’s Experiments
– When the “swan-necked flasks” remained upright, no microbial growth appeared
– When the flask was tilted, dust from the bend in the neck seeped back into the flask and made the infusion cloudy with microbes within a day
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• The Scientific Method– Debate over spontaneous generation led in part to development
of scientific method– A group of observations leads scientist to ask question about
some phenomenon– The scientist generates hypothesis (potential answer to
question)– The scientist designs and conducts experiment to test
hypothesis– Based on observed results of experiment, scientist either
accepts, rejects, or modifies hypothesis
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• What Causes Fermentation?– Spoiled wine threatened livelihood of vintners, compelling them
to fund research into how to promote production of alcohol but prevent spoilage by acid during fermentation
– Some believed air caused fermentation reactions, while others insisted living organisms caused fermentation
– This debate also linked to debate over spontaneous generation
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• What Causes Disease?– Pasteur developed germ theory of disease– Robert Koch studied causative agents of disease
– Anthrax– Examined colonies of microorganisms
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Koch’s Experiments– Simple staining techniques– First photomicrograph of bacteria– First photomicrograph of bacteria in diseased tissue– Techniques for estimating CFU/ml– Use of steam to sterilize media– Use of Petri dishes– Aseptic techniques– Bacteria as distinct species
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• Koch’s Postulates
– Suspected causative agent must be found in every case of the disease and be absent from healthy hosts
– Agent must be isolated and grown outside the host– When agent is introduced into a healthy, susceptible host, the
host must get the disease– Same agent must be reisolated from now-diseased experimental
host
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
• How Can We Prevent Infection and Disease?– Semmelweis and handwashing– Lister’s antiseptic technique– Nightingale and nursing– Snow – infection control and epidemiology– Jenner’s vaccine – field of immunology– Ehrlich’s “magic bullets” – field of chemotherapy
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Golden Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• What Are the Basic Chemical Reactions of Life? – Biochemistry
– Began with Pasteur’s work on fermentation and Buchner’s discovery of enzymes in yeast extract
– Kluyver and van Niel – microbes used as model systems for biochemical reactions
– Practical applications– Design of herbicides and pesticides– Diagnosis of illnesses and monitoring of patients’
responses to treatment– Treatment of metabolic diseases– Drug design
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• How Do Genes Work?– Microbial genetics– Molecular biology– Recombinant DNA technology– Gene therapy
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• Microbial Genetics– Avery, MacLeod, and McCarty determined genes are contained
in molecules of DNA– Beadle and Tatum established that a gene’s activity is related to
protein function– Translation of genetic information into protein explained– Rates and mechanisms of genetic mutation investigated– Control of genetic expression by cells described
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• Molecular Biology– Explanation of cell function at the molecular level– Genome sequencing– Pauling proposed that gene sequences could
– Provide understanding of evolutionary relationships and processes
– Establish taxonomic categories that reflect these relationships
– Identify existence of microbes that have never been cultured– Woese determined that cells belong to bacteria, archaea, or
eukaryotes– Cat-scratch fever caused by unculturable organism
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• Recombinant DNA Technology
– Genes in microbes, plants, and animals manipulated for practical applications
– Production of human blood-clotting factor by E. coli to aid hemophiliacs
• Gene Therapy
– Inserting a missing gene or repairing a defective one in humans by inserting desired gene into host cells
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• What Role Do Microorganisms Play in the Environment?
– Bioremediation uses living bacteria, fungi, and algae to detoxify polluted environments
– Recycling of chemicals such as carbon, nitrogen, and sulfur
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• How Do We Defend Against Disease?– Serology
– The study of blood serum– Von Behring and Kitasato – existence in the blood of
chemicals and cells that fight infection– Immunology
– The study of the body’s defense against specific pathogens– Chemotherapy
– Fleming discovered penicillin– Domagk discovered sulfa drugs
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings
The Modern Age of Microbiology
• What Will the Future Hold?
– Microbiology is built on asking and answering questions– The more questions we answer, the more questions we have