ChangeYourGenes–ChangeYourLifeSor2ngHopefromHypeinGene2csGlobalWellnessSummit(GWS)

KennethR.Pelle2er,PhD,MDClinicalProfessorofMedicine

ClinicalProfessorofFamily&CommunityMedicineProfessorofPublicHealth

UniversityofArizonaSchoolofMedicineUniversityofCaliforniaSchoolofMedicine,

SanFrancisco(UCSFMed)

Epigene2csEra

•  HealthBiomarkersvsDiseasePredicDon

•  Genomics

–  Studyoftheen#regenome(Sequencing,Mapping&InteracDons)

•  Epigenomics

–  Studyofpost-translaDonalgeneDcsmodificaDons

•  Metabolomics(GeneDcExpression)

–  Studyofthecompletesetofmetabolitesorsmallmolecules(MetabolicIntermediates,Byproducts,Hormones,SignalingCompounds)presentinacellororganism

•  Microbiome:Studyofallthemicrobesinandonaperson

Epigene2cs:ATripar2teAssay

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SingleNucleo2dePolymorphisms:CommonGene2cVariants

SNPs

•  SNPsactasarheostattoexpressorsuppressgeneDcpredisposiDon•  GeneDcVariaDon:centraltopersonalizedmedicine

•  SNPsinfluencebydiet/nutriDon,stress/meditaDon,radiaDon,physicalandpsychosocial.environment,Rxs,andsenseofpurpose

•  >3millionSNPsidenDfied

•  EsDmatedpotenDal10+millionSNPsinthehumangenome

•  SNPedia:83,452referenceSNPs

A Tripartite Assay Pilot Study Stool Microbiome, Pathogens, and Infectious Agents Among Olympic and Elite Athletes

PURPOSE: Olympic athletes, non-Olympic elite athletes, and other active adults may have genetic and metabolic profiles that are unique. These profiles may contribute to the caliber of their performance, and they may provide keys to understanding select genetic and metabolic conditions.

For further details: Ms Kathrin E. Nikolussi Chief Business Development Officer kathrin.nikolussi@wellnessfx.com

WhatDoWeTest?

•  WholegenomebasemappingusingnextgeneraDonsequencingtechnology

•  SNPbasedandwholegenebasedanalyDcsGene2cs

•  Morethan100keybloodbiomarkerscoveringallkeyhealthandwellnessproblemsBlood

•  Genomesequencingbasedmicrobiomeandmetabolomicsanalysisforupto300keybiomarkersMicrobiome

Gene2cbiomarkerselec2oncriteria:

1.  GenebiomarkersarestablefromoneDmeoftesDngtothenextbythesamelaboratory.GenesarestableunlessthereisaspecificintervenDonthataltersthem;

2.  ChangescanbemadeintheexpressionofthesegenesbyacDonable,modifiable,selfcarelifestyleintervenDons;

3.  ChangescanbedetectedinamaximumDmeframeof10-12weeksalthoughmanychangeinamaberofhoursordays;and

4.  Arecommerciallyavailable.

EPIGENESISResearchProject

EPIGENESIS:7PillarsofOp2malHealth

•  Methyla2on: Methyl(CH3)moleculeswithgenesonandofftogovernDNAexpression

•  Inflamma2on: Acutevs.chronicanddestrucDve

•  Oxida2veStress: ExcessiveoxidaDon(PON1andSOD2)

•  Detoxifica2on: PhaseoneandPhasetwobreakdownandexcreDonoftoxins

•  Immunity: DifferenDateselffromnotselfwithhypo-andhyper-immunity

•  LipidMetabolism: GeneDcexpressionsgovernopDmallipidmetabolism

•  MineralMetabolism:

Governmetabolismofnutrientsandtraceelementfromwholefoods

Inflamation Moderate

IBD High

Lactose Intolerance Low

Gluton Intolerance Low

There are certain known microbial signatures that are closely associated with a number of conditions. Ut iriure tincidunt consectetuer tation macto immitto praemitto ludus venio. Luptatum minim autem lobortis vindico fatua autem epulae. Dolore paratus olim plaga dolore duis aliquip demoveo importunus importunus defui meus. Ratis roto ad neque wisi ut opto.

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Phyla LevelRelative Abundance & Diversity

Important Genus & Species Diversity

Relative Abundance

Genus

Diversity

Firmicutes

Bacteroides

Evenness

Richness

Normal

Normal

Moderate

Good

Tenericutes Normal

Bacteroidetes Low

Cyanobacteria LowAmino Acid that aids in…

Proteobacteria Low

Fusobacteria PresentBreaks down food

Actinobacteria Normal

Verrucomicrobia High

Enterobacteriaceae Enterobacteriaceae

HbA1c HbA1c

Ruminococcaceae Ruminococcaceae

Gene GC,rs2282679

Gene GC,rs2282679

Folate Folate

RBC Magnesium RBC Magnesium

Vitamin D Vitamin D

alpha diversity alpha diversity

Example Phenotype Signature Your Signature

Hypothetical Example

Summary Your DataFactors Score Signature Map

Your data compared to Healthy/BaselineFactors

21 % down11 % up5 % downInsignificant DifferenceInsignificant Difference15 % down25 % down

Enterobacteriaceae

HBA1c

Ruminococcaceae

Gene GC, rs 2282679

Folate

RBC Magnesium

Alpha Diversity

Important Factors for this Phenotype

Type2Diabetes

Enterobacteriaceae

HbA1c

Ruminococcaceae

GeneGC,rs2282679presence

2hoursOralintakeBloodGlucose

PPAR-RVitaminD

FasDngBloodGlucose

Microbiome

Gene2csBlood

DATAANALYTICS:GENETICS,BLOOD,ANDMICROBIOME

THEMICROBIOME:KEYTOLIFE

ThereareaboutonetrillionbacterialivingintheGItract,ten2meshigherthanthetotalamountofcellscomposing

thehumanbody.

GItract

Reproduc2vetract

Skin

EyesEars

SystemSummaryFounda2on1

Inges2onSensorFounda2on2

WearableSensorFounda2on3

MobileApplica2ons

Whatitiden2fies• Thataspecificpill,tabletorotheringestedproduct(orcombinaDon)wasingested.

• ComposedandpoweredenDrelyfrommaterialsfoundinthedailydiet.

Whatitsenses• PreciseDme&idenDtyofingesDons.

• CertainphysiologicresponsesandconsumerbehaviorsoverDme.

– HR,HRV,acDvity,sleep,temperature.

• ActsascommunicaDonshubbetweeningestedproductandphone.

Whatitinfluences• Consumerreportedwellnessmetrics.

• CorrelaDonsbetweeningesDonadherence,patchphysiologicmeasures,anddatafromothertelemetricdevices.

• EnablecollaboraDonwithcliniciansandcaregivers.

TrackingAdherenceEnablesCarerstoIden2fyPa2entsWhoNeedHelp

ACCESSIBLE,SIMPLEBLOODCOLLECTION,ANYWHERE

VirtuallypainlessNobigneedlesSimpletouseCanbeself-administered

NocoldchainrequiredEaseofshipping

Anywhere,AnyDmeSampling

Epigene2cs–WhatisKnown

•  TripartateAssay:GeneDc,Blood(CBC),andMicrobiome

•  GenespredictprobabiliDesnotcertainDes

•  BiomarkersofhealthnotdiseasepredicDon

•  ApplicaDonsofsinglegene=singlediseaseisverylimited

•  GenesworkwithincomplexgeneDcandenvironmentalmatrices

•  Humanbaseis@21,000genes–DNAforproteincodingisonly5%ofthisenDregenome=“DarkGenome”

•  Genesareturnedonorofflikearheostat

•  Geneschange–Whatwedomabers

•  Majorityofgenesgovernedbybeliefsandlifestylechoices

•  Neanderthalgenesarealiveandwell–StressResponses

1.GeneswithNoDirectMolecularCorrelateinBlood

2.GeneswithDirectMolecularCorrelatesinBlood

3.GeneswithDirectPathway/NetworkCorrelatesinBlood

4.GeneswithClinical&MolecularPhenotypeCorrelates

FOURDISTINCTCLASSES

Genes-CBC