chapter 1

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ABSTRACT The problem was to find the effect of water impurities on plant growth. Each plant will be watered with sugar, salt, bleach, or water. Which water impurity will affect the plant's growth? How will each water impurity effect the plant's growth? To do this experiment, one plant has to be watered with 157.6 milliliters of water, another watered with 157.6 milliliters of a sugar and water solution, the third plant has to be watered with 157.6 milliliters of a salt and water solution, and the fourth plant has to be watered with 157.6 milliliters of bleach and water solution. The plants were watered everyday in the morning, and measured the stems every other day. The heights were recorded and the experiment ran for four weeks. The conclusions for this experiment are that the plant watered with sugar grew the most, while the plant Page 1

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Page 1: Chapter 1

ABSTRACT

The problem was to find the effect of water impurities on plant growth. Each

plant will be watered with sugar, salt, bleach, or water. Which water

impurity will affect the plant's growth? How will each water impurity effect

the plant's growth? To do this experiment, one plant has to be watered with

157.6 milliliters of water, another watered with 157.6 milliliters of a sugar

and water solution, the third plant has to be watered with 157.6 milliliters of

a salt and water solution, and the fourth plant has to be watered with 157.6

milliliters of bleach and water solution. The plants were watered everyday

in the morning, and measured the stems every other day. The heights were

recorded and the experiment ran for four weeks. The conclusions for this

experiment are that the plant watered with sugar grew the most, while the

plant watered with water grew a little less. The plant watered with salt grew

even less, and the plant watered with bleach grew the least. The heights of

each plant changed by going up or down. No matter what substance was

used to water the plant, it had to be mixed into water. Otherwise it wouldn't

dissolve into the soil.

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Chapter 1

Problem and It’s Background

Introduction

This experiment is "The effect of water impurities on the growth of

plants." It will determine the effect of sugar, salt, and bleach on the growth

of a plant. Which water impurity will effect a plants growth? Will each

substance used affect the rate of the plants growth differently?

Objective

The purpose of this experiment is to find out what water impurity will

affect the plant the most.

Hypothesis

The hypothesis of the researcher is. if a plant is watered with sugar,

then it will grow the most.

Significance of the Study

The purpose of this experiment was to determine the effect of water

impurities on the growth of plants.

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Scope and Delimitation

Scopes:

The experiment will focus on the effect of water impurities on plant’s

growth. It will focus on which will affect the plant the most.

Limit:

The researcher limited this research to the effect of water impurity on

plant’s growth.

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Definition of Terms

Bleach - A substance that will be mixed with water, and will be used

to water the plants.

Salt - A substance that will be mixed with water, and will be used to

water the plants.

Sugar - A substance that will be mixed with water, and will be used to

water the plants.

Water - Will be used to water the plants.

Munggo beans – The seed that will be used in the experiment.

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Chapter 2

Review of Related Literature

A. Review of the literature.

Salt is a mineral that is composed primarily of sodium chloride. It is

essential for animal life in small quantities, but is harmful to animals and

plants in excess. Salt is one of the oldest, most ubiquitous food seasonings

and salting is an important method of food preservation. The taste of salt

(saltiness) is one of the basic human tastes.

Salt for human consumption is produced in different forms: unrefined

salt (such as sea salt), refined salt (table salt), and iodized salt. It is a

crystalline solid, white, pale pink or light gray in color, normally obtained

from sea water or rock deposits. Edible rock salts may be slightly grayish in

color because of mineral content.

Chloride and sodium ions, the two major components of salt, are

needed by all known living creatures in small quantities. Salt is involved in

regulating the water content (fluid balance) of the body. However, too much

salt increases the risk of health problems, including high blood pressure.

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Therefore health authorities have recommended limitations of dietary

sodium.

Different natural salts have different mineralities, giving each one a

unique flavor. Fleur de sel, natural sea salt harvested by hand, has a

unique flavor varying from region to region. In traditional Korean cuisine,

so-called "bamboo salt" is prepared by roasting salt [13] in a bamboo

container plugged with mud at both ends. This product absorbs minerals

from the bamboo and the mud, and has been shown to increase the

anticlastogenic and antimutagenic properties of doenjang.[14]

Completely raw sea salt is bitter because of magnesium and calcium

compounds, and thus is rarely eaten. The refined salt industry cites

scientific studies saying that raw sea and rock salts do not contain enough

iodine salts to prevent iodine deficiency diseases.[15]

Unrefined sea salts are also commonly used as ingredients in bathing

additives and cosmetic products. One example is bath salts, which uses

sea salt as its main ingredient and combined with other ingredients used for

its healing and therapeutic effects.

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Refined salt, which is most widely used presently, is mainly sodium

chloride. Food grade salt accounts for only a small part of salt production in

industrialised countries (3% in Europe) although worldwide, food uses

account for 17.5% of salt production. The majority is sold for industrial use.

Salt has great commercial value because it is a necessary ingredient in the

manufacturing of many things. A few common examples include: the

production of pulp and paper, setting dyes in textiles and fabrics, and the

making of soaps and detergents.

The manufacture and use of salt is one of the oldest chemical

industries. Salt can be obtained by evaporation of sea water, usually in

shallow basins warmed by sunlight; salt so obtained was formerly called

bay salt, and is now often called sea salt or solar salt. Rock salt deposits

are formed by the evaporation of ancient salt lakes and may be mined

conventionally or through the injection of water. Injected water dissolves

the salt, and the brine solution can be pumped to the surface where the salt

is collected.

After the raw salt is obtained, it is refined to purify it and improve its

storage and handling characteristics. Purification usually involves

recrystallization. In recrystallization, a brine solution is treated with

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chemicals that precipitate most impurities (largely magnesium and calcium

salts). Multiple stages of evaporation are then used to collect pure sodium

chloride crystals, which are kiln-dried.

Since the 1950s it has been common practice in the United Kingdom

to add a trace amount of sodium ferrocyanide to the brine; this acts as an

anticaking agent by promoting irregular crystals. The safety of sodium

ferrocyanide as a food additive was confirmed in the United Kingdom in

1993. Some anticaking agents used are tricalcium phosphate, calcium or

magnesium carbonates, fatty acid salts (acid salts), magnesium oxide,

silicon dioxide, calcium silicate, sodium aluminosilicate, and calcium

aluminosilicate. Both the European Union and the United States Food and

Drug Administration (FDA) permitted the use of aluminum in the latter two

compounds. The refined salt is then ready for packing and distribution

Table salt is refined salt, which contains about 97% to 99% sodium

chloride. It usually contains substances that make it free-flowing (anti-

caking agents) such as sodium silicoaluminate or magnesium carbonate.

Some people also add a desiccant, such as a few grains of uncooked rice

in salt shakers to absorb extra moisture and help break up clumps when

anti-caking agents are not enough. Table salt has a particle density of

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2.165 g/cm3, and a bulk density (dry, ASTM D 632 gradation) of about

1.154 g/cm

In many East Asian cultures, salt is not traditionally used as a

condiment.[31] However, condiments such as soy sauce, fish sauce and

oyster sauce tend to have a high salt content and fill much the same role as

a salt-providing table condiment that table salt serves in western cultures.

Sodium is one of the primary electrolytes in the body. All four cationic

electrolytes (sodium, potassium, magnesium, and calcium) are available in

unrefined salt, as are other vital minerals needed for optimal bodily

function. Too much or too little salt in the diet can lead to muscle cramps,

dizziness, or electrolyte disturbance, which can cause neurological

problems, or death. Drinking too much water, with insufficient salt intake,

puts a person at risk of water intoxication (hyponatremia). Salt is

sometimes used as a health aid, such as in treatment of dysautonomia.

Salt intake can be reduced by simply reducing the quantity of salty

foods in a diet, without recourse to salt substitutes. Salt substitutes have a

taste similar to table salt and contain mostly potassium chloride, which will

increase potassium intake. Excess potassium intake can cause

hyperkalemia. Various diseases and medications may decrease the body's

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excretion of potassium, thereby increasing the risk of hyperkalemia. Those

who have kidney failure, heart failure or diabetes should seek medical

advice before using a salt substitute. One manufacturer, LoSalt, has issued

an advisory statement that those taking the following prescription drugs

should not use a salt substitute: amiloride, triamterene, Dytac,

spironolactone (Aldactone), and eplerenone (Inspra).

Salt, also known as table salt, or rock salt, is a mineral that is

composed primarily of sodium chloride. It is essential for animal life in small

quantities, but is harmful to animals and plants in excess. Salt is one of the

oldest, most ubiquitous food seasonings and salting is an important method

of food preservation. The taste of salt (saltiness) is one of the basic human

tastes.

Salt for human consumption is produced in different forms: unrefined

salt (such as sea salt), refined salt (table salt), and iodized salt. It is a

crystalline solid, white, pale pink or light gray in color, normally obtained

from sea water or rock deposits. Edible rock salts may be slightly grayish in

color because of mineral content.

Chloride and sodium ions, the two major components of salt, are

needed by all known living creatures in small quantities. Salt is involved in

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regulating the water content (fluid balance) of the body. However, too much

salt increases the risk of health problems, including high blood pressure.

Therefore health authorities have recommended limitations of dietary sodiu

Salt is a water soluble mineral that is vital to life. It is made up of

sodium and chlorine, both of which play very important roles in good health.

Without salt, the fluids in the human body could not be kept in their proper

balance.

Salt is also a very important as an industrial chemical. Common salt

a chemical combination of sodium and chlorine, is found in rocks

throughout the world. Water is continually dissolving tiny amounts of salt

from rocks and soil. Streams carry the dissolved salt to the sea, and every

day the heat of the sun evaporates some of the water, leaving the salt

behind. In this way the sea has slowly been growing saltier and for million

years.

An increasing amount of salt is collected from nature each year. The

present world-wide collection rate has reached nearly 150 million metric

tons. Leading countries in the production of salt include the United States,

China, Ukraine , Turkmenistan, Germany, and India. Salt is obtained from

salt mines, brine walls, and the sea.

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In mining salt a shaft is sunk through the earth until the salt bed is

reached. Miners then dig into the wall and use explosives to blast loose

salt. The chunks of salt are loaded on small freight cars, trucks, or

conveyor belts and carried to the surface. Then the rock salt is put through

crushers and reduced to small particles.

Many kinds of animal search for salt deposits that lie at the earth’s

surface. These deposits are called licks because the animals lick the rock

salt with their tongues. Many of the trails made by the animals on their way

to lick came to be traveled by people. Some roads now in use were once

animal salt trails. Today animals on farms are given feed with salt mixed

into it. Blocks of pressed salt are sometimes put out in the pastures.

The recomended intake of salt for healthy adults is ½ to 1½

teaspoon a day. Most of this is found naturally in foods .Salt sold for table

use often has iodine added to it to prevent a disease called goiter.

Many medical researchers believe that too much salt in the diet can

lead to hypertension or high blood pressure. This is a condition in which

blood vessels constrict, or tighten. As a result, blood is pumped through the

vessels with a dangerous amount of force.

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Not everyone develops hypertension from eating too much salt.but

people who already have high blood pressure can usually improve their

condition with a low-salt diet.

Only a small amount of the world’s total salt production goes into

foods. Most of it is used by the chemical industry. In industrial nations, as

much as 70 percent of the salt is put some chemical use.

Salt is called a basic chemical that means it is used in the production

of many other chemicals, such as caustic soda (sodium hydroxide), used to

make soap and paper. Chemicals made from salt are important in the

manufacture of glass, synthetic fabric, leather, and fertilizer. Liqiud sodium ,

made from salt, plays an important part in nuclear power plants as a

cooling agent. Salt also goes into the manufacture of textile dyes and

explosives.

In 2004, Britain's Food Standards Agency started a public health

campaign called "Salt - Watch it", which recommends no more than 6g of

salt per day; it features a character called Sid the Slug and was criticised

by the Salt Manufacturers Association (SMA). The Advertising Standards

Authority did not uphold the SMA complaint in its adjudication. In March

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2007, the FSA launched the third phase of their campaign with the slogan

"Salt. Is your food full of it?" fronted by comedienne Jenny Eclair.

The University of Tasmania's Menzies Research Institute maintains a

website to educate people about the problems of a salt-laden diet.

Consensus Action on Salt and Health (CASH) established in 1996,

actively campaigns to raise awareness of the harmful health effects of salt.

The 2008 focus includes raising awareness of high levels of salt hidden in

sweet foods and marketed towards children.

Taxation of sodium has been proposed as a method of decreasing

sodium intake and thereby improving health in countries like the United

States where typical salt consumption is high.

The Salt Institute, a salt industry body, is active in promoting the use

of salt, and questioning or opposing restrictions on salt intake.

Diets low in salt are mainly low sodium diets, that is, diets that

specifically aim to lower intake of sodium, potentially including salt

substitutes replacing sodium with other components.

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Sugar is a term for a class of edible crystalline carbohydrates, mainly

sucrose, lactose, and fructose characterized by a sweet flavor. In food,

sugar almost exclusively refers to sucrose, which primarily comes from

sugar cane and sugar beet. Other sugars are used in industrial food

preparation, but are usually known by more specific names—glucose,

fructose or fruit sugar, high fructose corn syrup, etc.

Currently, Brazil has the highest per capita production of sugar.

Sugar, because of its simpler chemical structure, was once assumed

(without scientific research) to raise blood glucose levels more quickly than

starch, but results from more than twenty studies demonstrate that sugar

and starch cause blood glucose to rise at similar rates. This finding showed

that controlling all carbohydrates is necessary for controlling blood glucose

levels, the idea behind carbohydrate counting. Experts now agree that

eating too much sugar does not cause diabetes. Excessive calories from

sugar, however, can lead to obesity, which may increase the risk of

diabetes. Sugars such as sucrose are known to contribute to tooth decay,

and it is impossible to develop cavities in the absence of fermentable

carbohydrates. The role of starches is disputed. Lower rates of tooth decay

have been seen in hereditary fructose intolerance.

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Scientifically, sugar loosely refers to a number of carbohydrates, such

as monosaccharides, disaccharides, or oligosaccharides. Monosaccharides

are also called "simple sugars," the most important being glucose. Almost

all sugars have the formula CnH2nOn (n is between 3 and 7). Glucose has

the molecular formula C6H12O6. The names of typical sugars end with "-

ose," as in "glucose", "dextrose", and "fructose". Sometimes such words

may also refer to any types of carbohydrates soluble in water. The acyclic

mono- and disaccharides contain either aldehyde groups or ketone groups.

These carbon-oxygen double bonds (C=O) are the reactive centers. All

saccharides with more than one ring in their structure result from two or

more monosaccharides joined by glycosidic bonds with the resultant loss of

a molecule of water (H2O) per bond.

Monosaccharides in a closed-chain form can form glycosidic bonds

with other monosaccharides, creating disaccharides (such as sucrose) and

polysaccharides (such as starch). Enzymes must hydrolyse or otherwise

break these glycosidic bonds before such compounds become

metabolised. After digestion and absorption. the principal monosaccharides

present in the blood and internal tissues include glucose, fructose, and

galactose. Many pentoses and hexoses can form ring structures. In these

closed-chain forms, the aldehyde or ketone group remains unfree, so many

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of the reactions typical of these groups cannot occur. Glucose in solution

exists mostly in the ring form at equilibrium, with less than 0.1% of the

molecules in the open-chain form.

Sugar is a water soluble substance. When people speak of sugars

they usually mean sucrose, or table sugar, which comes from sugarcane or

sugar beets. But there are also other kinds of sugar. all of them are

carbohydrates-that is, they are made up of carbon, hydrogen, and oxygen

arranged in different ways. A sugar’s sweetness and the speed it dissolves

in water depend upon its chemical structure.

Next to sucrose, the most commonly known sugar is dextrose, which

is also called glucose or grape sugar. Others are lactose, milk sugar;

maltose, malt sugar; and fructose (levulose), fruit sugar. Fructose is the

sugar you taste when you eat honey or most kind of fruits. It is many times

as sweet as sucrose. the form of fructose that is used in the manufacture of

bakery goods, confectionery items, preserves, and beverages is called high

fructose corn syrup (HFCS for short).

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Most fruits and vegetables contain sugars. In addition to simple

sugars, grains and dry seeds of plants contain a high percentage of starch,

which can be changed by chemical treatment or by the action of an

enzyme.

Sucrose is obtained from either sugarcane or sugar beets. Today

about 70 percent of world’s output of sugar is from sugarcane and about

30 percent from sugar beet.

Pure sucrose refined in the crystalline from with which we are familiar

is called granulated sugar. When sugar crystal are ground into a fine

powder, the sugar is called confectioners’ sugar. It is used to make cake

frosting and some candies.

Granulated sugar that is not refined all the way to pure whiteness is

called brown sugar. The brown color comes from the traces of molasses

that are still in the sugar. Brown sugars have a special flavor and fragrance

that white sugar does not have. They are used to sweeten cookies,

breads, and baked beans.

Syrup made from sugarcane is highly prized in the southern United

States, where it is used for cooking or as topping for pancakes. Molasses is

a product that is left over from the processes of sugar manufacture. This

heavy dark syrup may be as much as 50 percent sucrose, and it also has a

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high content of organic and mineral substances. The highest grades of

cane molasses are used to add flavor and sweetness to candies, cookies

and gingerbreads. The lower grades of molasses, are chiefly use for

livestock feed and by the distilling industries for alcohol protection.

Water is a chemical substance with the chemical formula H2O. Its

molecule contains one oxygen and two hydrogen atoms connected by

covalent bonds. Water is a liquid at ambient conditions, but it often co-

exists on Earth with its solid state, ice, and gaseous state, water vapor or

steam.

Water covers 70.9% of the Earth's surface, and is vital for all known

forms of life. On Earth, it is found mostly in oceans and other large water

bodies, with 1.6% of water below ground in aquifers and 0.001% in the air

as vapor, clouds (formed of solid and liquid water particles suspended in

air), and precipitation. Oceans hold 97% of surface water, glaciers and

polar ice caps 2.4%, and other land surface water such as rivers, lakes and

ponds 0.6%. A very small amount of the Earth's water is contained within

biological bodies and manufactured products.

Water on Earth moves continually through a cycle of evaporation or

transpiration (evapotranspiration), precipitation, and runoff, usually

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reaching the sea. Over land, evaporation and transpiration contribute to the

precipitation over land.

Clean drinking water is essential to human and other lifeforms.

Access to safe drinking water has improved steadily and substantially over

the last decades in almost every part of the world. There is a clear

correlation between access to safe water and GDP per capita. However,

some observers have estimated that by 2025 more than half of the world

population will be facing water-based vulnerability. A recent report

(November 2009) suggests that by 2030, in some developing regions of the

world, water demand will exceed supply by 50%. Water plays an important

role in the world economy, as it functions as a solvent for a wide variety of

chemical substances and facilitates industrial cooling and transportation.

Approximately 70% of freshwater is consumed by agriculture.

Water resources are sources of water that are useful or potentially

useful to humans. Uses of water include agricultural, industrial, household,

recreational and environmental activities. Virtually all of these human uses

require fresh water.

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97% of water on the Earth is salt water, and only 3% as fresh water of

which slightly over two thirds is frozen in glaciers and polar ice caps. The

remaining unfrozen freshwater is mainly found as groundwater, with only a

small fraction present above ground or in the air.

Fresh water is a renewable resource, yet the world's supply of clean,

fresh water is steadily decreasing. Water demand already exceeds supply

in many parts of the world and as the world population continues to rise, so

too does the water demand. Awareness of the global importance of

preserving water for ecosystem services has only recently emerged as,

during the 20th century, more than half the world’s wetlands have been lost

along with their valuable environmental services. Biodiversity-rich

freshwater ecosystems are currently declining faster than marine or land

ecosystems. The framework for allocating water resources to water users

(where such a framework exists) is known as water rights.

Water pollution is the contamination of water bodies (e.g. lakes,

rivers, oceans and groundwater).

Water pollution affects plants and organisms living in these bodies of

water; and, in almost all cases the effect is damaging not only to individual

species and populations, but also to the natural biological communities.

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Water pollution occurs when pollutants are discharged directly or

indirectly into water bodies without adequate treatment to remove harmful

compounds.

Water pollution is a major problem in the global context. It has been

suggested that it is the leading worldwide cause of deaths and diseases,

and that it accounts for the deaths of more than 14,000 people daily. An

estimated 700 million Indians have no access to a proper toilet, and 1,000

Indian children die of diarrheal sickness every day. Some 90% of China's

cities suffer from some degree of water pollution, and nearly 500 million

people lack access to safe drinking water. In addition to the acute problems

of water pollution in developing countries, industrialized countries continue

to struggle with pollution problems as well. In the most recent national

report on water quality in the United States, 45 percent of assessed stream

miles, 47 percent of assessed lake acres, and 32 percent of assessed bay

and estuarine square miles were classified as polluted.

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Water is typically referred to as polluted when it is impaired by

anthropogenic contaminants and either does not support a human use, like

serving as drinking water, and/or undergoes a marked shift in its ability to

support its constituent biotic communities, such as fish. Natural phenomena

such as volcanoes, algae blooms, storms, and earthquakes also cause

major changes in water quality and the ecological status of water.

The specific contaminants leading to pollution in water include a wide

spectrum of chemicals, pathogens, and physical or sensory changes such

as elevated temperature and discoloration. While many of the chemicals

and substances that are regulated may be naturally occurring (calcium,

sodium, iron, manganese, etc.) the concentration is often the key in

determining what is a natural component of water, and what is a

contaminant.

Oxygen-depleting substances may be natural materials, such as plant

matter (e.g. leaves and grass) as well as man-made chemicals. Other

natural and anthropogenic substances may cause turbidity (cloudiness)

which blocks light and disrupts plant growth, and clogs the gills of some fish

species.

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Many of the chemical substances are toxic. Pathogens can produce

waterborne diseases in either human or animal hosts. Alteration of water's

physical chemistry includes acidity (change in pH), electrical conductivity,

temperature, and eutrophication. Eutrophication is an increase in the

concentration of chemical nutrients in an ecosystem to an extent that

increases in the primary productivity of the ecosystem. Depending on the

degree of eutrophication, subsequent negative environmental effects such

as anoxia (oxygen depletion) and severe reductions in water quality may

occur, affecting fish and other animal populations.

Interactions between groundwater and surface water are complex.

Consequently, groundwater pollution, sometimes referred to as

groundwater contamination, is not as easily classified as surface water

pollution. By its very nature, groundwater aquifers are susceptible to

contamination from sources that may not directly affect surface water

bodies, and the distinction of point vs. non-point source may be irrelevant.

A spill or ongoing releases of chemical or radionuclide contaminants into

soil (located away from a surface water body) may not create point source

or non-point source pollution, but can contaminate the aquifer below,

defined as a toxin plume. The movement of the plume, a plume front, can

be part of a Hydrological transport model or Groundwater model. Analysis

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of groundwater contamination may focus on the soil characteristics and site

geology, hydrogeology, hydrology, and the nature of the contaminants.

Plants are living organisms belonging to the kingdom Plantae. They

include familiar organisms such as trees, herbs, bushes, grasses, vines,

ferns, mosses, and green algae. The scientific study of plants, known as

botany, has identified about 350,000 extant species of plants, defined as

seed plants, bryophytes, ferns and fern allies. As of 2004, some 287,655

species had been identified, of which 258,650 are flowering and 18,000

bryophytes (see table below). Green plants, sometimes called Viridiplantae,

obtain most of their energy from sunlight by a process called

photosynthesis .

Plants are one of the most abundant organism in the world. Without

plants , nearly all life on Earth would cease. Directly or indirectly, most

living things depend on plants for food. But more than that, we depend on

plants for thw very air we breathe. As plants perform their necessary life

processes, as they produce oxygen as a by-product. Human beings and

other land animals breathe the oxygen in the atmosphere; fish and other

water life breathe in the oxygen that is dissolved in the water. So it is the

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abundance an diversity of plants that determine how much animal life,

including human life, can survive on earth.

Scientists estimate that there are more than 300,000 species, or

kinds , of plants. can be found in the humid tropics. In fact, there are so

many kind of plants in the tropics that they have not yet been identified that

can only estimate how many species of plants inhabit the earth.

Regrettably, many plant species will become extinct before they have even

been seen. The fewest different plant species are found in the cold and

barren areas of the artic and artartic.

There is an amazing variety in size and appearance of plants;

however, there are basic characteristics that all plants share: Plants are

made up of many cell. Each cell is surrounded by a solid cell wall made up

of Cellulose. Each cell also has organ like structures called plastids, where

many of the biochemical reactions of energy use and energy storage occur.

Most plant make their own food during the process of photosynthesis.

Plants also produce embryos- young plants that develop from an egg,

or ovum. Plants are nonmotile; that is, they don’t move like animals do.

When scientist classify the many different species of plants, they

separate them into groups, depending on the characteristics the plants

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share. Major groups , called divisions, include plants that share share one

or more characteristics. Plants within division are separated into groups

called classes according to certain differences between the plants . The

classes are further divided into orders, the orders into families, the families

into genera, and the genera into species.

With each subdivision, the relation between the plants become

stronger, and the members of the group share more characteristics of

appearance, body structure,a nd development . With the final seperation

into species, the plants share so many of the same basic characterisrtics

that they look almost alike.

Along with scientific classification, plants can be put into two basic

groups: vascular and nonvascular. Vascular plants are those plants that

have specialized tissues to transport materials from one part of the plant to

another. Tissue composed of xylem conducts water throughout the plant ,

and tissue composed of phloem conducts food . Seed plants, ferns, whisk

ferns, horsetails, and club mosses are thosee plants that do not have

specialized tissues to transport materials Bryophytes, which include

liverworts, hornworts , and mosses, are nonvascular plants.

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Seed plants are vascular plants that, along with their specialized

tissues, have an additional unique structure- the seed. Seed plants are

seperated into two groups: gymnosperms, plants with naked seeds, and

angiosperms, plants with seed encolsed in fruit. The principal gymnosperm

are the conifers, trees that beer seeds on a female cone; the male cones

produce pollen. Conifers are very important ecologically, forming the

dominant vegetation in cold, moist regions.

Most of the solid material in a plant is taken from the atmosphere.

Through a process known as photosynthesis, most plants use the energy in

sunlight to convert carbon dioxide from the atmosphere, plus water, into

simple sugars. Parasitic plants, on the other hand, use the resources of its

host to grow. These sugars are then used as building blocks and form the

main structural component of the plant. Chlorophyll, a green-colored,

magnesium-containing pigment is essential to this process; it is generally

present in plant leaves, and often in other plant parts as well.

Plants usually rely on soil primarily for support and water (in

quantitative terms), but also obtain compounds of nitrogen, phosphorus,

and other crucial elemental nutrients. Epiphytic and lithophytic plants often

depend on rainwater or other sources for nutrients and carnivorous plants

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supplement their nutrient requirements with insect prey that they capture.

For the majority of plants to grow successfully they also require oxygen in

the atmosphere and around their roots for respiration. However, some

plants grow as submerged aquatics, using oxygen dissolved in the

surrounding water, and a few specialized vascular plants, such as

mangroves, can grow with their roots in anoxic conditions.

The genotype of a plant affects its growth, for example selected

varieties of wheat grow rapidly, maturing within 110 days, whereas others,

in the same environmental conditions, grow more slowly and mature within

155 days.

Growth is also determined by environmental factors, such as

temperature, available water, available light, and available nutrients in the

soil. Any change in the availability of these external conditions will be

reflected in the plants growth.

Biotic factors are also capable of affecting plant growth. Plants

compete with other plants for space, water, light and nutrients. Plants can

be so crowded that no single individual produces normal growth. Optimal

plant growth can be hampered by grazing animals, suboptimal soil

composition, lack of mycorrhizal fungi, and attacks by insects or plant

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diseases, including those caused by bacteria, fungi, viruses, and

nematodes.

Simple plants like algae may have short life spans as individuals, but

their populations are commonly seasonal. Other plants may be organized

according to their seasonal growth pattern: annual plants live and

reproduce within one growing season, biennial plants live for two growing

seasons and usually reproduce in second year, and perennial plants live for

many growing seasons and continue to reproduce once they are mature.

These designations often depend on climate and other environmental

factors; plants that are annual in alpine or temperate regions can be

biennial or perennial in warmer climates. Among the vascular plants,

perennials include both evergreens that keep their leaves the entire year,

and deciduous plants which lose their leaves for some part of it. In

temperate and boreal climates, they generally lose their leaves during the

winter; many tropical plants lose their leaves during the dry season.

The growth rate of plants is extremely variable. Some mosses grow

less than 0.001 millimeters per hour (mm/h), while most trees grow 0.025-

0.250 mm/h. Some climbing species, such as kudzu, which do not need to

produce thick supportive tissue, may grow up to 12.5 mm/h.

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Plants protect themselves from frost and dehydration stress with

antifreeze proteins, heat-shock proteins and sugars (sucrose is common).

LEA (Late Embryogenesis Abundant) protein expression is induced by

stresses and protects other proteins from aggregation as a result of

desiccation and freezing.

A bleach is a chemical that removes colors or whitens, often via

oxidation. Common chemical bleaches include household chlorine bleach,

a solution of approximately 3–6% sodium hypochlorite (NaClO), and

oxygen bleach, which contains hydrogen peroxide or a peroxide-releasing

compound such as sodium perborate, sodium percarbonate, sodium

persulfate, tetrasodium pyrophosphate, or urea peroxide together with

catalysts and activators, e.g., sodium nonanoyloxybenzenesulfonate.

Bleaching powder is calcium hypochlorite.

Many bleaches have strong bactericidal properties, and are used for

disinfecting and sterilizing. Bleach is sold extremely concentrated and must

be diluted to be used safely when disinfecting surfaces and when used to

treat drinking water. When disinfecting most surfaces, 1 part bleach to 9

parts water is sufficient for sanitizing. In an emergency, drinking water can

be treated: Ratio of bleach to water for purification: 2 drops of bleach per

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litre of water or 8 drops of bleach per gallon (4L) of water; 1/2 teaspoon

bleach per five gallons (19L) of water. If water is cloudy, filter the water

before adding the bleach. Additional bleach will not kill more bacteria and

can endanger health.

Color in most dyes and pigments are produced by molecules,

such as beta carotene, which contain chromophores. Chemical bleaches

work in one of two waysAn oxidizing bleach works by breaking the

chemical bonds that make up the chromophore. This changes the molecule

into a different substance that either does not contain a chromophore, or

contains a chromophore that does not absorb visible light. A reducing

bleach works by converting double bonds in the chromophore into single

bonds. This eliminates the ability of the chromophore to absorb visible light.

Sunlight acts as a bleach through a process leading to similar results:

high energy photons of light, often in the violet or ultraviolet range, can

disrupt the bonds in the chromophore, rendering the resulting substance

colorless. Extended exposure often leads to massive discoloration usually

reducing the colors to white and typically very faded blue spectrums.

Sodium hypochlorite's anti-bacterial mechanism works by causing

proteins to aggregate.

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The broad-spectrum effectiveness of bleach, particularly sodium

hypochlorite, owes to the nature of its chemical reactivity with microbes.

Rather than acting in an inhibitory or toxic fashion in the manner of

antibiotics, bleach quickly reacts with microbial cells to irreversibly denature

and destroy many pathogens. Bleach, particularly sodium hypochlorite has

been shown to react with a microbe's heat shock proteins, stimulating their

role as intra-cellular chaperone and causing the bacteria to form into

clumps (much like an egg that has been boiled) that will eventually die off.

In some cases, bleach's base acidity compromises a bacterium's lipid

membrane, a reaction similar to popping a balloon. The range of micro-

organisms effectively killed by bleach (particularly sodium hypochlorite) is

extensive, making it an extremely versatile disinfectant. In response to

infection, the human immune system will produce a strong oxidizer,

hypochlorous acid, to kill bacterial invaders.

Bleach is highly toxic to fish and invertebrates. In confined spaces,

fish will attempt to swim away from the source. Ill-considered use may

create resistant micro-organisms, both at the site of application and at the

disposal site, leading to aggravated health problems.

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High levels of absorbable organic halides (AOX) can be found during

reaction of sodium hypochlorite and soils, including carbon tetrachloride,

trihalomethanes (THM, such as chloroform), and trihaloacetic acid (THAA,

in this case trichloroacetic acid). Most AOX go into the sewer with wash

water.

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Chapter 3

Methodology

A. Procedure

Preparing the pot and Planting the seed.

First we cut 4 bottles of 1.5 coke. We drilled some holes at the bottom

of each bottle. Then we put an average amount of soil in each bottle

needed for the experiment. We digged holes in the soil in each bottle . We

poured hot water to the soil to kill bacterias and other organisms that my

prohibit the growth of plant. We placed the seeds inside the holes that we

digged.

Labeling the Plants.

We labled each bottle A,B,C,D. The plants are placed where they can

get equal sunlight. The labels are needed to determine what solution the

plants are suppose to receive.

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Preparing the solution

Each plant was watered with different kind of solution. For the sugar

solution, We mixed 3 cup of water and 3 tbsp of sugar. For the salt solution,

We mixed 3 cups of water and 3tbsp of salt. For the bleach solution, We

mixed 3 cups of water and 1 cup of bleach.

Watering the plants.

We watered the plants according to their label. Bottle A was watered

with bleach. Bottle B was watered with a solution of water and sugar. Bottle

C was watered with a solution of salt and water, and bottle D was watered

with water. Each plant was watered equally. The plants were watered

everyday in the morning.

Measuring the stems

We measured the stems every other day. After measuring the stems,

we will record our observation. We will observe the plants for 1 month or 30

days.

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Recording the data

We recorded the data according to the label of the plant. we will

record the growth of the stem to determine which plant grew the highest fo

the past one month.

Analyzing the data table

The days are written at the top part of the table. The plants are written at

the left side of the table. Under each day, we recorded the height of the

plant. At the end of the month, we will observe which plant grew the most

and which plant grew the least.

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B. Data Table

Table 1 (Bleach Solution)

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

A

PLANT

D

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Table 2 (Sugar Solution)

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

B

PLANT

D

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TABLE 3 (SALT SOLUTION)

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

C

PLANT

D

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Chapter 4

Discussion of Results

Results

The results of the plant watered with water ( Plant D) were that it

grew by one centimeter from the first to fifth day, its height went down by

one fourth of a centimeter from the fifth to eighth day, its height was the

same from the eighth to twelfth day, and it grew by three fourths of a

centimeter from the twelfth to fifteenth day. The plants height stayed the

same from the fifteenth to the twenty-ninth day.

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TABLE 1 (BLEACH SOLUTION)

The first plant’s height, watered with bleach (Plant A), grew by one fourth of a centimeter from the first to

fifth

day, its height stayed the same from the fifth to the twelfth day. Its height stayed the same from the

twelfth day to the nineteenth day. Its height grew by half of a centimeter from the nineteenth to the

twenty-second day. Its height stayed the same from the nineteenth day up to the last day.

TABLE 2 (SUGAR SOLUTION)

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

B

1 cm 1 cm 1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

.1.25

cm

1.25

cm

1.75

cm

1.75

cm

1.75

cm

1.75

cm

PLANT

D

1 cm 1cm .75

cm

.75

cm

.75

cm

.75

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

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The

second plant watered with sugar (Plant B) grew one centimeter from the first to fifth day, it grew one

fourth of a centimeter from the fifth to eighth day, its height stayed the same from the eighth to the

twenty –sixth day. The plant grew one fourth of a centimeter from the twenty-sixth to the twenty-ninth

TABLE 3 (SALT SOLUTION)

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

B

1 cm 1 cm 1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

.1.25

cm

1.25

cm

1.75

cm

1.75

cm

1.75

cm

1.75

cm

PLANT

D

1 cm 1cm .75

cm

.75

cm

.75

cm

.75

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

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The third plant watered with salt ( Plant C )grew one and one fourth of a centimetre from the first to fifth

day, its height went down by half of a centimetre from the fifth to eighth day, it grew half of a centimetre

from the eighth to twelfth day, and the plants height stayed the same from the twelfth to the nineteenth

day. The plant grew half a centimetre from the nineteenth to the twenty-second day, and its height went

down by half of a centimetre from the twenty-second to the twenty-ninth day.

DAY

2

DAY

4

DAY

6

DAY

8

DAY

10

DAY

12

DAY

14

DAY

16

DAY

18

DAY

20

DAY

22

DAY

24

DAY

26

DAY

28

DAY

30

TOT

AL

PLANT

C

1.25

cm

1.25

cm

.75

cm

.75

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

1.75

cm

.1.75

cm

1.75

cm

1.25

cm

1.25

cm

1.25

cm

1.25

cm

PLANT

D

1 cm 1cm .75

cm

.75

cm

.75

cm

.75

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

1.5

cm

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Chapter 5

Summary, Conclusions, and Recommendations

Summary

The experiment was "The effect of water impurities on plant growth."

Some relationships were discovered. One was that as time went on, the

plants heights in some way changed (either it went up or down). No matter

what the plants were watered with it had to be mixed into water so it could

go into the soil and get to the plants roots. Information already known are

that plants need to be watered with some kind of liquid, and the plants

should be growing, and their height probably shouldn't be going down. The

conclusion was that the plant watered with sugar was the plant that grew

the most, overall. This is the conclusion because anything usable that

plants take in are turned into a form of sugar. The plant watered with sugar

in the water was already taking in sugar so it didn't have to convert as

many substances to a form of sugar. This conclusion was arrived at by

looking at the data and figuring out during what days of measurements

each plants height went down or up, and by how much. The plant watered

with sugar grew the most times (and grew the highest amount overall) in

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between the measurements taken. That is why that plant was the best

result of the experiment.

Conclusion

The conclusions of this experiment are that the plant watered with

sugar grew the most. Another conclusion is that the control (the plant

watered with water) grew a little less than the plant watered with sugar. The

plant watered with salt grew a little less than the control, and the plant

watered with bleach grew the least amount. The reason that was

discovered for why the plant watered with sugar grew the most was

because when plants absorb nutrients, they turn them into some form of

sugar. This plant didn't have to do that because it was already absorbing

sugar. The other substances, except water, probably just stopped the

plants from growing as much.

Recommendations

The next project to expand on the experiment that results in the effect

of water impurities on the growth of plants might be to use more water

impurities. There could also be a bigger variety of water impurities. The

plants used would still all be the same exact kind, but more of those plants

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would have to be bought. More research would have to be done because

there would have to be more reasons (because more water impurities are

being used) for why some water impurities didn't help the plants grow. This

research would also include why some water impurities did help the plants

grow. Besides, more research and the bigger varieties of substances used

to water the plants, the experiment would be done the same way.

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