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Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

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Page 1: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Chapter 10 Gene Isolation and Manipulation

Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

Page 2: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Finding specific nucleic acids by using gel electrophoresis and

blotting

Page 3: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 4: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 5: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 6: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 7: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 8: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 9: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Polymerase chain reaction (PCR)

Page 10: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 11: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 12: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 13: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 14: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Sequencing

Page 15: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

The structure of 2’,3’-dideoxynucleotides

Sanger Sequencing (Dideoxy Sequencing)

Page 16: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 17: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 18: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

The dideoxy sequencing method

Page 19: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Reading the DNA sequence from an automatic sequencer

Page 20: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

How to amplify an interesting gene

Page 21: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Making a cDNA clone

Page 22: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 23: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 24: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 25: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 26: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 27: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 28: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Introduction of a transgene

Page 29: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 30: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 31: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 32: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse
Page 33: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Agrobacterium tumefaciens

causal agent of Crown Gall

Page 34: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

The Ti plasmid vector

Page 35: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

The generation of a transgenic plant

Page 36: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

The generation of a transgenic plant

Page 37: Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse

Pattern of transmission of T-DNA


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