chapter 10sterilization failure ; 1-po(t) = 1 -[1-p(t)]no= 1 –[1 -exp(-k dt)]no sterilizationchart...
TRANSCRIPT
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Chapter 10
Bioreactor Type and Sterilization
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Type of bioreactors
Stirred-tank reactor
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Type of bioreactors
Bubble-column reactor
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Type of bioreactors
Airlift loop reactor
Propeller loop reactor
Jet loop reactor
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Materials for bioreactors
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When O2 transfer is limited or cell density is very high:
OUR = OTR
X qo2 = kL a (C* - CL)
OTR [mg O2 / L / h]
kL : oxygen transfer coefficient (cm/h)
a : gas-liquid interfacial area per unit vol. (cm2/cm3)
kL a : volumetric oxygen transfer coefficient (1/h)
C* : saturated DO concentration (mg/L)
CL : DO concentration in the broth (mg/L)
Aeration
When O2 transfer is limited or cell density is very high:
OUR = OTR
X qo2 = kL a (C* - CL)
OTR [mg O2 / L / h]
kL : oxygen transfer coefficient (cm/h)
a : gas-liquid interfacial area per unit vol. (cm2/cm3)
kL a : volumetric oxygen transfer coefficient (1/h)
C* : saturated DO concentration (mg/L)
CL : DO concentration in the broth (mg/L)
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How to determine kL a
Slope = “-kL a”
ln(C
* -C
L)
t
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How to determine kL aDynamic method
Bioreactor containing cells
2. Slope = OUR
CL
t1. Stopaeration
3. Resumeaeration
Determined from (4)
5. Finally calculated
known
MeasuredUsing DO probe
= OUR Determined
from (2)
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Reasons for sterilization
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Sterilization agents
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Kinetics of thermal sterilization
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Kinetics of thermal sterilization
P(t) = Probability that an individual cell is still viable at
sterilization time t
N(t) = # of individuals at time t
No = # of individuals at t=0
kd = specific death rate
P(t) = Probability that an individual cell is still viable at
sterilization time t
N(t) = # of individuals at time t
No = # of individuals at t=0
kd = specific death rate
P(t) = = exp(-kdt) N(t)No
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Temperature effects on kinetics of thermal sterilization
increasing temperature
-kd
(t)
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Population effects on kinetics of thermal sterilization
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Variables for thermal sterilization
Probability of extinction of total population ;
Po(t) = [1-P(t)]No
Probability of unsuccessful fermentation due to
sterilization failure ;
1- Po(t) = 1 - [1-P(t)]No = 1 – [1 - exp(-kdt)]No
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Sterilization chart1-
Po(
t)1-
Po(
t)
kdt
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Batch vs. continuous sterilization
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Batch vs. continuous sterilization
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Sterilization of gases