chapter 12 processing pre-mrna and nuclear transport by: rachael hattrup
TRANSCRIPT
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Chapter 12Processing pre-mRNA and
Nuclear TransportBy:
Rachael Hattrup
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Processing of eukaryotic Pre-mRNA
1) 5’ capping with 7-methylguanosine2) 3’cleavage at Poly (A) site3) Polyadenylation added
1) 250 in mammals2) 150 insects3) 100 yeasts
4) RNA splicingIntrons removed and exons kept.
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5’ Capping
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Overview of mRNA processing in Eukaryotes
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Macromolecular Transport into Nucleus
• Nuclear envelope
• Nuclear Pores– Nuclear pore complex (NPC)– Nucleoporins
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Nuclear Pore Complex
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Macromolecular Transport into Nucleus
1) 2 Importins bind to create nuclear-import receptor
2) -Importin binds to Nuclear-localization signal (NLS) of “cargo” protein
2) -importin interacts with FG nuclear proteins to diffuse through NPC
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Macromolecular Transport into Nucleus cont…
3) Ran-GTP interacts with importins causing conformational change creating less affinity for NLS
4) Ran-GTP transports importin out to cytoplasm
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Transport into Nucleus
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Macromolecular Transport out of Nucleus
1) Protein exportin 1 binds to Nuclear-export signal (NES) bound to “cargo” protein and Ran-GTP
2) Difusses through NPC
3) Ran-GTP converted to Ran-GDP releasing the complex
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Transport out of Nucleus
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The End
Questions?????