chapter 21 cytokinins: a diffusible and water-soluble regulators of cell division autoclaved herring...
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Chapter 21 Cytokinins:
a diffusible and water-soluble regulators of cell division
autoclaved herring sperm DNA immature endosperm of corn (banana)
Common structure: N6-substituted aminopurines,
the structural requirements for activity
No kinetin-induced cell division occurs without auxin in the culture medium.
Cytokinins definition:
have biological activities similar to those of trans-zeatin, including
¤ induce cell division in callus cells in the presence of an auxin
¤ promote bud and root formation from callus cultures when in the
appropriate molar ratios to auxin
¤ delay senescence of leaves
¤ promote expansion of dicot cotyledons
¤ cell proliferation
¤ chloroplast differentiation
¤ nutrient mobilization
Callus: grow as a mass of disorganized, relatively undifferentiated cells
wounding inducible
de-differentiated
Crown gall: a specific type of callus, the wounding site of plants was
invaded by Agrobacterium tumefaciens, it cause the neoplastic
(tumor-forming) disease.
Sources:
yeast extract, tomato juice, banana extract, coconut milk,
transfer-RNA— hypermodified bases
Conjugated cytokinins:
¤ N3, N7, N9 with sugars
riboside: N9 attach sugar ribotide: ribose sugar moiety contains a phosphate group glycoside: even the oxygen of zeatin.
¤ is inactive form
¤ glucosidase remove the
conjugation at the side chain
¤ Dormant seeds:
high levels of cytokinin glucosides
Witches’ broom on balsam fir,
Induced by Corynebacterium fascians
Some plant pathogenic bacteria, insects, and nematodes secrete free cytokinins
Isopentenyl transferase
Dimethylally diphosphate1-hydroxy-2-methyl-2-butenyl 4-diphosphate
古森本
The sites of cytokinins biosynthesis:
root apical meristems: root-derived cytokinin in shoot development, in general
young maize embryos, young developing leaves, young fruits
the difference of physiological significance
The transported form: zeatin ribosides in the xylem exudate
The degradation of cytokinins: cytokinin oxidase
but not for O-glucoside derivatives