chapter 5: microbial metabolism. metabolism sum of all chemical rxn’s within a living organism...
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METABOLISMMETABOLISM
• Sum of all chemical rxn’s within a living organism
• Release energy• Require energy
CATABOLIC REACTION (degradative)
• Breakdown of complex organic compounds into simpler ones
• Generally hydrolytic reactions (water used to break bonds)
• Exergonic (produce more energy than consume)
• Provide building blocks for anabolic rxns and energy needed to drive anabolic rxns
ANABOLIC REACTIONS (biosynthetic)
• Building of complex organic molecules from simpler ones
• Generally dehydration synthesis
• Endergonic (consume more energy than produce)
ATP <=> ADP + Pi + energy
Rxn’s all determined by enzymesand coupled together
Enzymes• Biological catalysts• Each act on a specific substance (substrate)• Has active site for substrate to bind to
– “loading dock”• Each catalyzes only one reaction• Sensitive to temperature, pH, concentration• Can become denatured• All end in -ase
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Enzymes• Large 3-D globular molecule
• Composed of two parts1. Protein portion (apoenzyme)– Inactive if alone
2. Nonprotein component (cofactor)– Ions of iron, zinc, magnesium or
calcium– If organic = coenzyme
• Together with the apoenzyme and cofactor = active enzyme (holoenzyme)
HOW DO YOU
CONTROL GROWTH OF BACTERIA??
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InhibitorsCompetitive Inhibitors• Fill the active site of an enzyme
and compete with the normal substrate for the active site
• Similar shape and chemical structure
• Does not produce products• Example: Sulfa drug
Animation: CD
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InhibitorsNoncompetitive Inhibitors• Do not compete with the substrate; instead interact with
another part of the enzyme• Binds and causes the active site to change its shape, making
it nonfunctional• Allosteric inhibition “other space”Animation: CD
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Carbohydrate Catabolism• Most microorganisms use
carbon as primary energy source
• Oxidation of glucose1. Cellular
Respiration Glycolysis Krebs cycle Electron Transport Chain
2. Fermentation Glycolysis Products: Alcohol or lactic
acid
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Fermentation• Releases energy
from sugars or other organic molecules
• Does not require oxygen
• Produces only a small amount of ATP
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Alcohol Fermentation: •Saccharomyces (yeast)
Heterolactic: produce both
Lactic Acid Fermentation• Homolactic: only make lactic acid•Streptococcus• Lactobacillus• Bacillus
Lipid and Protein Catabolism
LIPIDS• Produce enzymes called lipases• Break down into fatty acid and glycerol• Put into the Kreb cycle• Also use this to break down petroleum products -
oil spills
PROTEINS• Produce proteases
and peptidases• Convert proteins into
different substance and enter Kreb cycle
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• Conversion of light energy into chemical energy• Chemical energy then used to convert CO2 into
sugars (carbon fixation)• Cyanobacteria• Animation
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REVIEW
• Cellular Respiration– Aerobic– Carbon
• Fermentation– Anaerobic– Carbon
• Photosynthesis– Carbon Fixation– Light energy
• Protein Catabolism– Use Kreb Cycle
• Lipid Catabolism– Use Kreb Cycle
Classifying Bacteria-Nutritional Pattern
HOW DO THEY GET
ENERGY?Phototrophs: use light as
primary energy sourceChemotrophs: use
reactions of inorganic or organic compounds for energy
HOW DO THEY GET
CARBON?
Autotrophs: CO2 as principle carbon source
Heterotrophs: organic carbon source
PHOTOAUTOTROPHS
Energy: light
Carbon: CO2
Examples: cyanobacteria
Green and Purple Bacteria - use sulfur or hydrogen gas to reduce CO2 and make organic compounds, not H20
Do not produce O2
(Anoxygenic)
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Chromatium -Purple BacteriaFound in sulfide-containing freshwaterhabitats
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Chlorobium- Green Bacteriafound in hotsprings, cold lakesand sediments
PHOTOHETEROTROPHS
Energy: LightCarbon: organic compoundsAnoxygenic
Examples:Green nonsulfur bacteria
Chloroflexus (found in hot springs, lakes,
hyersaline environments)
Purple nonsulfur bacteriaRhodopseudomonas
(found in soil and marine environments)
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CHEMOAUTOTROPHS
Energy: inorganic compounds
Ex: H2S, S, NH3, H2, CO
Carbon: CO2
-fix CO2
Example:
Beggiatoa - use H2S, found in soil, sulfur springs, mud layers of lakes,
Deep Sea Vents http://www.teachersdomain.org/asset/tdc02_vid_deepseavents/
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CHEMOHETEROTROPHS
Energy: organic compoundsEx: glucose
*use the electrons from H-atoms as energy source
Carbon: organic compound*Hard to distinguish-use the same
compound
Example:Streptococcus pneumonia - fermentation
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• http://people.eku.edu/ritchisong/RITCHISO/energyflowchart.jpg
• http://www.hepafilters.com/images/microbes.jpg• http://www.bio12.com/ch6/RemedialEnzymes_file
s/image007.jpg• http://classes.midlandstech.edu/carterp/Courses/bi
o225/chap05/Slide13.GIF