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Chapter 5 Replication 235-266

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Chapter 5

Replication

235-266

Replication

Semi conservative replication

Page 4: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Synthesis in a 5’ to 3’ direction

Page 5: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

DNA polymerase

Page 6: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

DNA polymerase

It must be perfect!!

Page 7: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

WRONG!!

Page 8: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

The replication fork

Understand why there is a leading strand and a lagging strand

Page 9: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Mistakes happen!

Page 10: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Chew back and do it again

Page 11: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Priming the replication reaction in the lagging strand

Page 12: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Synthesizing the lagging strand

Page 13: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Helicase

Forcing the double stranded DNA apart!

Page 14: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

The replication fork

Page 15: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

The “actual” replication fork

Page 16: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

SEM of replication fork

•

Page 17: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

What releases the rotational pressure on the DNA?

Page 18: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Topoisomerase

Page 19: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

What happens to the histones?

Page 20: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

Histones are evenly shared between daughter strands, and new histones fill in the gaps

Page 21: Chapter 5 - Queen's Universitypost.queensu.ca/~biol330/Chapter 5-replication.pdf · Chapter 5 Replication 235-266. Replication. Semi conservative . ... Okazaki fragment) part 1 of

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