chapter 6 1 biology and society:...
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© 2013 Pearson Education, Inc.Lectures by Edward J. Zalisko
PowerPoint® Lectures forCampbell Essential Biology, Fifth Edition, and
Campbell Essential Biology with Physiology,
Fourth Edition– Eric J. Simon, Jean L. Dickey, and Jane B. Reece
Chapter 6Chapter 6Cellular Respiration: Obtaining
Energy from Food
1 Biology and Society: Marathoners versus Sprinters
• Sprinters do not usually compete at short and long
distances.
• Natural differences in the muscles of these athletes
favor sprinting or long-distance running.
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5
Figure 6.0 6
• The muscles that move our legs contain two main
types of muscle fibers:
1. slow-twitch and
2. fast-twitch.
Biology and Society: Marathoners versus Sprinters
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• Slow-twitch fibers
– last longer,
– do not generate a lot of quick power, and
– generate ATP using oxygen (aerobically).
Biology and Society: Marathoners versus Sprinters
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• Fast-twitch fibers
– contract more quickly and powerfully,
– fatigue more quickly, and
– can generate ATP without using oxygen (anaerobically).
• All human muscles contain both types of fibers but
in different ratios.
Biology and Society: Marathoners versus Sprinters
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9
ENERGY FLOW AND CHEMICAL CYCLING IN THE BIOSPHERE
• Animals depend on plants to convert the energy of
sunlight to
– chemical energy of sugars and
– other organic molecules we consume as food.
• Photosynthesis uses light energy from the sun to
– power a chemical process and
– make organic molecules.
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10 Producers and Consumers
• Plants and other autotrophs (self-feeders)
– make their own organic matter from inorganic nutrients.
• Heterotrophs (other-feeders)
– include humans and other animals that cannot make organic molecules from inorganic ones.
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11
Producers and Consumers
• Autotrophs are producers because ecosystems
depend upon them for food.
• Heterotrophs are consumers because they eat
plants or other animals.
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12 Chemical Cycling between Photosynthesis and Cellular Respiration
• The ingredients for photosynthesis are carbon
dioxide (CO2) and water (H2O).
– CO2 is obtained from the air by a plant’s leaves.
– H2O is obtained from the damp soil by a plant’s roots.
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• Chloroplasts in the cells of leaves use light energy
to rearrange the atoms of CO2 and H2O, which
produces
– sugars (such as glucose),
– other organic molecules, and
– oxygen gas.
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Chemical Cycling between Photosynthesis and Cellular Respiration
14
• Plant and animal cells perform cellularrespiration, a chemical process that
– primarily occurs in mitochondria,
– harvests energy stored in organic molecules,
– uses oxygen, and
– generates ATP.
Chemical Cycling between Photosynthesis and Cellular Respiration
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• The waste products of cellular respiration are
– CO2 and H2O,
– used in photosynthesis.
Chemical Cycling between Photosynthesis and Cellular Respiration
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• Animals perform only cellular respiration.
• Plants perform
– photosynthesis and
– cellular respiration.
Chemical Cycling between Photosynthesis and Cellular Respiration
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• Plants usually make more organic molecules than
they need for fuel. This surplus provides material
that can be
– used for the plant to grow or
– stored as starch in potatoes.
Chemical Cycling between Photosynthesis and Cellular Respiration
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18 Figure 6.2
Sunlight energyenters ecosystem
Photosynthesis
Cellular respiration
C6H12O6 CO2
drives cellular work
Heat energy exits ecosystem
ATP
O2 H2O
19
CELLULAR RESPIRATION: AEROBIC HARVEST OF FOOD ENERGY
• Cellular respiration is
– the main way that chemical energy is harvested from food and converted to ATP and
– an aerobic process—it requires oxygen.
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• Cellular respiration and breathing are closely
related.
– Cellular respiration requires a cell to exchange gases with its surroundings.
– Cells take in oxygen gas.
– Cells release waste carbon dioxide gas.
– Breathing exchanges these same gases between the blood and outside air.
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CELLULAR RESPIRATION: AEROBIC HARVEST OF FOOD ENERGY
21
Figure 6.3
Breathing
Cellularrespiration
Musclecells
Lungs
CO2
CO2
O2
O2
22 The Simplified Equation for Cellular Respiration
• A common fuel molecule for cellular respiration is
glucose.
• Cellular respiration can produce up to 32 ATP
molecules for each glucose molecule consumed.
• The overall equation for what happens to glucose
during cellular respiration is
– glucose & oxygen � CO2, H2O, & a release of energy.
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23
Figure 6.UN01
C6H12O6 CO2 ATPO2 H2O
Glucose Oxygen Carbondioxide
Water Energy
6 66
24 The Role of Oxygen in Cellular Respiration
• During cellular respiration, hydrogen and its
bonding electrons change partners from sugar to
oxygen, forming water as a product.
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Redox Reactions
• Chemical reactions that transfer electrons from one substance to another are called
– oxidation-reduction reactions or
– redox reactions for short.
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• Why does electron transfer to oxygen release
energy?
– When electrons move from glucose to oxygen, it is as though the electrons were falling.
– This “fall” of electrons releases energy during cellular respiration.
Redox Reactions
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Figure 6.4
Releaseof heat
energy
1
2H2 O2
H2O
30
• Cellular respiration is
– a controlled fall of electrons and
– a stepwise cascade much like going down a staircase.
Redox Reactions
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• The path that electrons take on their way down from glucose to oxygen involves many steps.
• The first step is an electron acceptor called NAD+.
– NAD is made by cells from niacin, a B vitamin.
– The transfer of electrons from organic fuel to NAD+ reduces it to NADH.
NADH and Electron Transport Chains
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• The rest of the path consists of an electron transport chain, which
– involves a series of redox reactions and
– ultimately leads to the production of large amounts of ATP.
NADH and Electron Transport Chains
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Figure 6.5
Electrons from food
Stepwise releaseof energy usedto make
Hydrogen, electrons,and oxygen combineto produce water
NADHNAD++++
H++++
H++++
ATP
H2O
O2
2 2
2
2
2
1
e−−−− e−−−−
e−−−− e−−−−
e−−−−
e−−−−
34 Figure 6.5a
Stepwise release
of energy used
to make ATP
Hydrogen, electrons, and oxygen combine to produce water
H++++
ATP
H2O
O2
2 2
2
2
21
e−−−−
e−−−−
Electron transport chain
H++++
35
An Overview of Cellular Respiration
• Cellular respiration is an example of a metabolic
pathway, which is a series of chemical reactions in
cells.
• All of the reactions involved in cellular respiration
can be grouped into three main stages:
1. glycolysis,
2. the citric acid cycle, and
3. electron transport.
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36
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BioFlix Animation: Cellular Respiration
37
Figure 6.6
Cytoplasm
Cytoplasm
Cytoplasm
Animal cell Plant cell
Mitochondrion
Mitochondrion
High-energyelectronsvia carriermolecules
CitricAcidCycle
ElectronTransport
Glycolysis
Glucose
2Pyruvic
acid
ATP ATP ATP
38 Figure 6.6a
CytoplasmMitochondrion
High-energyelectronsvia carrier
molecules
Citric
Acid
Cycle
Electron
Transport
Glycolysis
Glucose2
Pyruvic
acid
ATP ATP ATP
39
The Three Stages of Cellular Respiration
• With the big-picture view of cellular respiration in
mind, let’s examine the process in more detail.
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40 Stage 1: Glycolysis
1. A six-carbon glucose molecule is split in half to
form two molecules of pyruvic acid.
2. These two molecules then donate high energy
electrons to NAD+, forming NADH.
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41
Figure 6.7
Energy investment phase
Carbon atom
Phosphate group
High-energy electron
Key
Glucose
2 ATP2 ADP
INPUT
P
–
Energy harvest phase
OUTPUT
NADH
NADH
NAD++++
NAD++++ 2 ATP
2 ATP
2 ADP
2 ADP
2 Pyruvic acid
P
PP
P
PP
P
P
1
2
2
3
3
– –
– –
42 Figure 6.7a
2 Pyruvic acid
Glucose
INPUT OUTPUT
43
Figure 6.7b-1
Energy investment phase
2 ATP2 ADP
P
P1
44 Figure 6.7b-2
Energy investment phase
2 ATP2 ADP
Energy harvest phase
NADH
NADH
NAD++++
NAD++++P
PP
P
PP
P
P– –
– –
2
2
1
45
Figure 6.7b-3
Energy investment phase
2 ATP2 ADP
Energy harvest phase
NADH
NADH
NAD++++
NAD++++ 2 ATP
2 ATP
2 ADP
2 ADP
P
PP
P
PP
P
– –
– –
2
2
3
3
1
P
46
– uses two ATP molecules per glucose to split the six-carbon glucose and
– makes four additional ATP directly when enzymes transfer phosphate groups from fuel molecules to ADP.
• Thus, glycolysis produces a net of two molecules
of ATP per glucose molecule.
3. Glycolysis
Stage 1: Glycolysis
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Figure 6.8
ADPATP
P
P P
Enzyme
48Stage 2: The Citric Acid Cycle
• In the citric acid cycle, pyruvic acid from glycolysis
is first “groomed.”
– Each pyruvic acid loses a carbon as CO2.
– The remaining fuel molecule, with only two carbons left, is acetic acid.
• Oxidation of the fuel generates NADH.
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• Finally, each acetic acid is attached to a molecule
called coenzyme A to form acetyl CoA.
• The CoA escorts the acetic acid into the first
reaction of the citric acid cycle.
• The CoA is then stripped and recycled.
Stage 2: The Citric Acid Cycle
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50 Figure 6.9
(from glycolysis)
(to citric acid cycle)
Oxidation of the fuelgenerates NADH
Pyruvic acidloses a carbon
as CO2
Acetic acidattaches tocoenzyme APyruvic acid
Acetic acid Acetyl CoA
Coenzyme A
CoA
CO2
NAD++++ NADH
INPUT OUTPUT2
31
– –
51
Figure 6.9a
(from glycolysis)
(to citric acid cycle)
Pyruvic acidAcetyl CoA
CoA
INPUT OUTPUT
52 Figure 6.9b
Oxidation of the fuelgenerates NADH
Pyruvic acidloses a carbonas CO2
Acetic acidattaches tocoenzyme A
Acetic acid
Coenzyme ACO2
NAD++++ NADH
OUTPUT
1
2
3
– –
53
• The citric acid cycle
– extracts the energy of sugar by breaking the acetic acid molecules all the way down to CO2,
– uses some of this energy to make ATP, and
– forms NADH and FADH2.
Stage 2: The Citric Acid Cycle
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54
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Blast Animation: Harvesting Energy: Krebs Cycle
Select “Play”
55
Figure 6.10
3 NAD++++
ADP ++++ P
3 NADH
FADH2FAD
Aceticacid
Citricacid
Acceptormolecule
CitricAcidCycle
ATP
2 CO2
INPUT OUTPUT
3
12
4
5
– –
– –
6
56 Figure 6.10a
3 NAD++++
ADP ++++ P
3 NADH
FADH2FAD
Aceticacid
1
ATP
2 CO2
INPUT OUTPUT
4
2
3
5
– –
– –
57
Figure 6.10b
Citricacid
Acceptormolecule
CitricAcidCycle
INPUT OUTPUT
58
• Electron transport releases the energy your cells need to make the most of their ATP.
• The molecules of the electron transport chainare built into the inner membranes of mitochondria.
• The chain
– functions as a chemical machine, which
– uses energy released by the “fall” of electrons to pump hydrogen ions across the inner mitochondrial membrane, and
– uses these ions to store potential energy.
Stage 3: Electron Transport
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• When the hydrogen ions flow back through the
membrane, they release energy.
– The hydrogen ions flow through ATP synthase.
– ATP synthase
– takes the energy from this flow and
– synthesizes ATP.
Stage 3: Electron Transport
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60 Figure 6.11
Space betweenmembranes
Innermitochondrialmembrane
Electroncarrier
Proteincomplex
Electronflow
Matrix Electron transport chain ATP synthase
NADH NAD++++
FADH2 FAD
ATPADP
H2OO21
2
H++++
2
P
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++ H++++
H++++
H++++
H++++
H++++1
2
4
6
53
– –
– –
61
1
2
Figure 6.11a
Spacebetweenmembranes
Innermitochondrialmembrane
Electroncarrier
Proteincomplex
Electronflow
Matrix Electron transport chain ATP synthase
NADH NAD++++
FADH2 FAD
ATPADP
H2OO2 2
P
– –
– –
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++ H++++
H++++
H++++
H++++
1
2
4
6
53
H++++
62
1
2
Figure 6.11b
Spacebetweenmembranes
Inner
mitochondrial
membrane
Electron
carrier
Protein
complex
Electron
flow
Matrix Electron transport chain
NADH NAD++++
FADH2 FAD
O2
H++++
2
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H
––
––
1
2
3
4
63
1
2
Figure 6.11c
ATP synthase
ATPADP
H2OO2 2
P
H++++
5
4
6
H++++
H++++
H++++
H++++
H++++
H++++
H++++
H++++++++
64
• Cyanide is a deadly poison that
– binds to one of the protein complexes in the electron transport chain,
– prevents the passage of electrons to oxygen, and
– stops the production of ATP.
Stage 3: Electron Transport
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The Results of Cellular Respiration
• Cellular respiration can generate up to 32
molecules of ATP per molecule of glucose.
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66 Figure 6.12
Cytoplasm
Mitochondrion
NADH
CitricAcidCycle
ElectronTransport
Glycolysis
Glucose
2Pyruvic
acid
2ATP
2ATP
NADHNADH
FADH2
Maximumper
glucose:
2AcetylCoA
About28 ATP
by directsynthesis
by directsynthesis
by ATPsynthase
2 2
2
6
About32 ATP
– –– –
– –
– –
67
Figure 6.12a
CitricAcidCycle
ElectronTransport
Glycolysis
Glucose2
Pyruvicacid
2ATP
2ATP
2AcetylCoA
About28 ATP
by directsynthesis
by directsynthesis
by ATPsynthase
68
• In addition to glucose, cellular respiration can
“burn”
– diverse types of carbohydrates,
– fats, and
– proteins.
The Results of Cellular Respiration
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69
Figure 6.13
Food
Polysaccharides Fats Proteins
Sugars Glycerol Fatty acids Amino acids
Glycolysis Acetyl
CoA
Citric
Acid
Cycle
ElectronTransport
ATP
70 FERMENTATION: ANAEROBIC HARVEST OF FOOD ENERGY
• Some of your cells can actually work for short
periods without oxygen.
• Fermentation is the anaerobic (without oxygen)
harvest of food energy.
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Fermentation in Human Muscle Cells
• After functioning anaerobically for about 15
seconds, muscle cells begin to generate ATP by
the process of fermentation.
• Fermentation relies on glycolysis to produce ATP.
• Glycolysis
– does not require oxygen and
– produces two ATP molecules for each glucose broken down to pyruvic acid.
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• Pyruvic acid, produced by glycolysis,
– is reduced by NADH,
– producing NAD+, which
– keeps glycolysis going.
• In human muscle cells, lactic acid is a by-product.
Fermentation in Human Muscle Cells
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73
© 2013 Pearson Education, Inc.
Animation: Fermentation Overview
Right click slide / select “Play”
74 Figure 6.14
Glucose
2 ATP
2 NAD++++ 2 NADH 2 NADH 2 NAD++++
2 H++++
2 ADP
2 Pyruvic acid2 Lactic acid
Glycolysis
INPUT OUTPUT
2 P
– – – –
75
• Observation: Muscles produce lactic acid under anaerobic conditions.
• Question: Does the buildup of lactic acid cause muscle fatigue?
The Process of Science: What Causes Muscle Burn?
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• Hypothesis: The buildup of lactic acid would cause muscle activity to stop.
• Experiment: Tested frog muscles under conditions when lactic acid
– could and
– could not diffuse away.
The Process of Science: What Causes Muscle Burn?
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77
Figure 6.15
Battery
Forcemeasured
Battery
Forcemeasured
Frog muscle
stimulated by electric
current
Solution preventsdiffusion of lactic acid
Solution allowsdiffusion of lactic acid;
muscle can work fortwice as long
++++ –++++ –
78
• Results: When lactic acid could diffuse away,
performance improved greatly.
• Conclusion: Lactic acid accumulation is the
primary cause of failure in muscle tissue.
• However, recent evidence suggests that the role of
lactic acid in muscle function remains unclear.
The Process of Science: What Causes Muscle Burn?
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79
Fermentation in Microorganisms
• Fermentation alone is able to sustain many types
of microorganisms.
• The lactic acid produced by microbes using
fermentation is used to produce
– cheese, sour cream, and yogurt,
– soy sauce, pickles, and olives, and
– sausage meat products.
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• Yeast is a microscopic fungus that
– uses a different type of fermentation and
– produces CO2 and ethyl alcohol instead of lactic acid.
• This type of fermentation, called alcoholic fermentation, is used to produce
– beer,
– wine, and
– breads.
Fermentation in Microorganisms
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81
Figure 6.16
Glucose
2 ATP
2 NADH 2 NAD++++
2
++++ 2 P
2 Pyruvicacid
2 Ethyl alcohol
Glycolysis
INPUT OUTPUT
2 CO2 released
2 ADP
H++++++++
2 NADH2 NAD++++
– –– –
82 Evolution Connection:Life before and after Oxygen
• Glycolysis could be used by ancient bacteria to
make ATP
– when little oxygen was available, and
– before organelles evolved.
• Today, glycolysis
– occurs in almost all organisms and
– is a metabolic heirloom of the first stage in the breakdown of organic molecules.
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Figure 6.17
O2
pre
sen
t in
Eart
h’s
atm
osp
here
First eukaryotic organisms
Origin of Earth
Atmospheric oxygen reaches 10% of modern levels
Atmospheric oxygenfirst appears
Oldest prokaryoticfossilsB
illi
on
s o
f years
ag
o 2.1
0
2.7
4.5
2.2
3.5
84