chapter 6 slides (part ii) ch490
TRANSCRIPT
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Enzymes
Example Mechanism: Chymotrypsin
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Chymotrypsin Example
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Chymotrypsin Mechanism
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Lysozyme Mechanism
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Lysozyme Mechanism
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Lysozyme Mechanism
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Enzymes
Transition State Analogs in Drug Design
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HIV Protease Transition State Analogs
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HIV Protease Transition State Analogs
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Enzymes
Kinetics
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What is Enzyme Kinetics & Why Study It?
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How to Do Enzyme Kinetic Measurements
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How to Describe Enzyme Kinetics
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Ideal Rate:
Michaelis-Menten Equation
Deviations due to:
o Limitation of measurements
o
Substrate inhibition
o Substrate prep contains inhibitors
o Enzyme prep contains inhibitors
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Enzymes
Michealis-Menten Kinetics
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Michaelis-Menten Plot
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VoDependence on Substrate Concentration [S]
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The Lineweaver-Burk Plot
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Derivation of Enzyme Kinetics Equation
Start with a model mechanism
Identify constraints and assumptions
Carry out algebra ...
Simplest Model Mechanism: E + S!
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E + Po One reactant, one product, no inhibitors
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Derivation of Enzyme Kinetics Equation
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