chapter2.pdf

explorer.bio-rad.comBiotechnology: A Laboratory Skills Course

Safety

Personal Protective Equipment Glasses vs. Goggles Gloves Lab Coat Face Shields

Safety Cabinets Orientation

Gas Shuts off Fire Extinguishers Fire blankets Eyewash/Shower

explorer.bio-rad.com

Laboratory Notebooks

Importance of recording actual data as it happens.

Formats Purpose

Biotechnology: A Laboratory Skills Course


Laboratory Notebooks

What to include Numbered

procedures Tables Calculations with all

math and units displayed

Sketches, model numbers etc

Signatures To and From



Equipment

Graduated Cylinders



Equipment

Volumetric Flasks



Equipment

Serological Pipet



Equipment

Pipet Pumps



Equipment

Transfer Pipets



Equipment

Burette



Equipment

Adjustable Micropipets



Equipment

Erlenmeyer flask



Equipment

Reagent Bottles



Equipment

Triple Beam Balance



Equipment

Electronic Balance



Equipment

Weigh Boats



Equipment

pH Meters



Equipment

Autoclave



How to Wash Glassware

Add small amount of soap and use a brush to make sure all surfaces are washed.

Rinse with tap water until no suds are evident. Normally at least 3 times.

Rinse twice with distilled water. Air dry or use oven to dry.



Labeling

Name of Solution Concentration Date made Name of person who

made it Potential Hazards if

necessary



Hazards

Universal Hazards Label



Significant Figures

Rules



Scientific Notation

Shorthand method to display numbers that convey their level of precision.

Uses a number that is multiplied by 10 raised to a positive or negative number.



Units of Measure



Making Solutions

Percent Solutions Mass to Volume



Making Solutions

Percent Solutions Volume to Volume



Making Solutions

Dilutions



Making Solutions

Molar Solutions Proportional Method



Making Solutions

Molar Solutions Unit Cancellation

Method



Diluting Molar Solutions


chapter2.pdf

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