chem 213 experiment: column chromatographychemistry.bd.psu.edu/justik/chem 213/chem 213...
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CHEM213Experiment:ColumnChromatography
Procedure:Youwillbeseparatingthemixturethatresultsfromthereactionbelow:
Inthisparticularexperiment,theoxidationisincompleteandyouhaveamixtureoffluoreneandfluorenonetoseparate.Objectives:
• Separatethetwocomponentsbycolumnchromatography.• Identifyeachcomponent
• Determinethemassofeachcomponent
Step1:Weighout~50mgofthemixture.Useabalanceoutsideofhoodtoeliminatetheindicatedmassfrombeingaffectedbytheairflow.FollowtheprocedurefromWilliamsonasfollows: