Cholesterol and My Gut Microbiome

Richard SpragueJuly 1, 2015

YouWeight

Human Non-Human

Cells

Human Non-Human

Genes

Human Non-Human

3 pounds

Find which microbes you have

Bacteroides Plebeius

Hacking my microbiome

May Jun Oct Jan Feb0

10000

20000

30000

40000

50000

60000

70000

Bifidobacterium

Did it help my sleep?

No

Microbiome changes over time

David et al: Host lifestyle affects human microbiota on daily timescalesGenome Biology 2014, 15:R8

April 21

April 28

Date Calories Carbs Fat ProteinCholesterol Sodium Sugars Fibre

1-Apr-15 2136 351 50 81 140 1687 86 282-Apr-15 1098 148 42 67 155 1374 49 253-Apr-15 221 27 8 8 35 153 17 04-Apr-15 1643 133 231 89 235 3430 42 185-Apr-15 2210 193 430 92 363 3455 76 136-Apr-15 1621 184 76 54 169 1450 111 67-Apr-15 1499 106 65 148 423 4307 49 128-Apr-15 1406 111 63 74 240 2264 47 119-Apr-15 1522 149 52 76 399 2395 17 15

10-Apr-15 1376 109 266 85 288 2372 5 1911-Apr-15 1454 192 42 67 313 2455 43 1712-Apr-15 1245 156 34 86 235 2089 46 1913-Apr-15 1190 101 50 117 328 814 70 814-Apr-15 1774 209 70 82 110 981 75 2515-Apr-15 1765 140 70 112 312 931 27 1516-Apr-15 2107 185 88 93 232 1568 59 2217-Apr-15 2355 297 240 125 248 1284 147 1518-Apr-15 2673 343 78 130 295 4132 101 1619-Apr-15 2099 203 95 89 245 1974 72 1020-Apr-15 1716 180 57 133 283 1888 65 2021-Apr-15 2099 145 109 123 201 2831 42 1722-Apr-15 2605 226 114 115 216 4017 88 2023-Apr-15 1688 197 231 93 222 2825 40 1924-Apr-15 1893 242 68 114 302 2232 83 1525-Apr-15 2908 398 101 105 295 4069 200 1926-Apr-15 3119 294 200 123 401 3003 40 1527-Apr-15 1386 191 50 88 199 723 55 1328-Apr-15 1713 187 66 87 225 2484 48 8

Firmicu

tes

Bactero

idetes

Proteobac

teria

Verruco

microbia

Unclassi

fied

Euryarch

aeota

Actinobac

teria0%

10%20%30%40%50%60%70%80%90%

One Week Change in my Microbiome

21-Apr 28-Apr

Macronutrients

Date Calories Carbs Fat Protein Cholesterol Sodium Sugars Fibre

Average (month) 1841.7 192.2 102.7 94.9 268.0 2298.5 64.1 15.2

Average (test period) 2242.6 241.9 124.7 108.7 262.3 2814.3 78.3 16.9

Difference from Ave 400.9 49.7 22.0 13.9 -5.7 515.7 14.2 1.6

% Diff from Ave 122% 126% 121% 115% 98% 122% 122% 111%

Microbes and cholesterol

Increased after one week

Tax_name ChangeRoseburia 41427

Faecalibacterium 33862Bacteroides 24346Lachnospira 13601Lactobacillus 9874

Same!

Next Steps

How you can start too

• Do multiple tests and do A/B experiments

• Download taxonomy data and convert to Excel

• Track key species• Tell everyone!

Thank you

@sprague

What to track May Jun Oct Jan Feb 21-Apr 28-Apr Faecalibacterium prausnitzii 99571 62316 5790 95382 136354 106400 165582 speciesRoseburia 13554 11157 7825 42782 10750 7952 49379 genusChristensenellaceae 82585 39713 40290 40977 13530 familyChristensenella 269 NA 38 17NA 126 92genusAkkermansia 30960 19654 7648 6269 NA 11611 9538 genusBifidobacteria Longum 32NA 1858 9571 2115 452 0speciesBifidobacterium 8473 6532 58747 66516 6919 7072 49genus B. Longum as % of total Bifido 0.38% NA 3.16% 14.39% 30.57% 6.39% 0.00% Bacteroidetes/Firmicutes ratio 41.09% 64.58% 47.09% 22.84% 21.93% 12.10% 19.74% Clostridium 35012 41679 71326 66114 17628 37684 2486 genusC. botulinum NA 25 128 NA 7 speciesC. clostridioforme 28902 35372 15170 24128 16747 speciesC. baratii 1223 NA 5588 30543 132 282 species

My microbiome through time

May-14 Jun-14 Oct-14 Jan-15 Feb-15 Apr-15 Apr-150%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

ActinobacteriaEuryarchaeotaUnclassifiedVerrucomicrobiaProteobacteriaBacteroidetesFirmicutes

May Jun Oct Jan Feb 21-Apr

28-Apr

0

20

40

60

80

100

120

140

160

180

Diversity through time

Phylum (unique)ClassOrderFamilyGenusSpecies

May Jun Oct Jan Feb 21-Apr

28-Apr

0%10%20%30%40%50%60%70%

Bifido/Firmicutes

Decreased after one weekTax_name Change

Firmicutes 127051Euryarchaeota 22647Actinobacteria 6852Verrucomicrobia 2213Chloroflexi -6Lentisphaerae -60Acidobacteria -82Synergistetes -1040Spirochaetes -2945Tenericutes -5530Proteobacteria -22617Bacteroidetes -37752

Increased after one weekTax_name Change

Bacteroidetes 37752 Proteobacteria 22617 Tenericutes 5530 Spirochaetes 2945 Synergistetes 1040 Acidobacteria 82 Lentisphaerae 60 Chloroflexi 6 Verrucomicrobia -2213 Actinobacteria -6852 Euryarchaeota -22647 Firmicutes -127051