chp column series - diaion.com · 20×150 20×250 4.6×150 4.6×250 10×150 20×150 20×250...

Analytical and preparative chromatography columns and materials for pharmaceutical applications5 Analytical and preparative chromatography columns and materials for pharmaceutical applications

MCI GEL™ CHP20/C04

Separation mechanism of CHP series

●Description of MCI GELTM columns and materials

C＝ColumnP＝Material

Matrix type Particle size

○Polymeric partition chromatography columns and materials MCI GEL™ CHP series

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�M�C�I� �G�E�L�T�M� �s�p�e�c�i�a�l�i�z�e�s� �i�n� �p�o�l�y�m�e�r�-�b�a�s�e�d� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s�.� �T�h�e� �u�s�e� �o�f� �p�o�l�y�m�e�r�-�b�a�s�e�d� �c�o�l�u�m�n�s� �h�a�s� �b�e�c�o�m�e� �m�o�r�e� �w�i�d�e�s�p�r�e�a�d� �t�h�a�n�k�s� �t�o� �t�h�e� �m�a�n�y� �a�d�v�a�n�t�a�g�e�s� �o�f� �t�h�e� �p�o�l�y�m�e�r� �m�a�t�r�i�x� �l�i�k�e� �e�x�c�e�l�l�e�n�t� �s�e�l�e�c�t�i�v�i�t�y�,� �t�h�e� �a�b�s�e�n�c�e� �o�f� �s�p�e�c�i�f�i�c� �a�d�s�o�r�p�t�i�o�n� �w�h�i�c�h� �i�s� �f�o�u�n�d� �c�o�m�m�o�n�l�y� �w�i�t�h� �s�i�l�i�c�a�-�b�a�s�e�d� �p�a�c�k�i�n�g�,� �o�p�e�r�a�b�i�l�i�t�y� �i�n� �a� �w�i�d�e� �p�H� �r�a�n�g�e� �a�n�d� �g�o�o�d� �c�h�e�m�i�c�a�l� �s�t�a�b�i�l�i�t�y� �d�u�e� �t�o� �t�h�e� �i�n�e�r�t� �n�a�t�u�r�e� �o�f� �p�o�l�y�m�e�r�i�c� �m�a�t�e�r�i�a�l�s�.� �T�h�e� �M�C�I� �G�E�L�T�M� �p�a�r�t�i�t�i�o�n� �c�h�r�o�m�a�t�o�g�r�a�p�h�y� �c�o�l�u�m�n�s� �a�r�e� �b�a�s�e�d� �o�n� �a� �p�o�l�y�s�t�y�r�e�n�e� �a�n�d� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�o�r�o�u�s� �p�o�l�y�m�e�r�.� �A�s� �R�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �a�p�p�l�i�e�d� �t�o� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �a� �w�i�d�e� �v�a�r�i�e�t�y� �o�f� �o�r�g�a�n�i�c� �c�o�m�p�o�u�n�d�s�,� �b�o�t�h� �i�n� �t�h�e� �i�s�o�c�r�a�t�i�c� �a�n�d� �g�r�a�d�i�e�n�t� �e�l�u�t�i�o�n� �m�o�d�e�.� �T�h�e� �c�o�m�p�o�u�n�d�s� �i�n�c�l�u�d�e� �p�e�p�t�i�d�e�s�,� �i�n�s�u�l�i�n�,� �s�m�a�l�l� �m�o�l�e�c�u�l�e� �A�P�I�s�,� �n�u�t�r�a�c�e�u�t�i�c�a�l� �c�o�m�p�o�u�n�d�s�,� �w�a�t�e�r�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s� �a�n�d� �n�u�c�l�e�o�t�i�d�e�s�.� �A�s� �N�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �u�s�e�d� �i�n� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �v�a�r�i�o�u�s� �c�a�r�o�t�e�n�o�i�d�s�,� �f�a�t�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s�,� �s�t�e�r�o�i�d�s�,� �a�n�d� �f�o�o�d� �a�d�d�i�t�i�v�e�s�.� �T�h�e�s�e� �c�o�l�u�m�n�s� �t�o�l�e�r�a�t�e� �v�a�r�i�o�u�s� �o�r�g�a�n�i�c� �s�o�l�v�e�n�t�s� �l�i�k�e� �h�e�x�a�n�e�,� �h�e�p�t�a�n�e�,� �m�e�t�h�y�l�e�n�e� �c�h�l�o�r�i�d�e�,� �a�n�d� �a�l�c�o�h�o�l�s�.� �A�s� �N�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �u�s�e�d� �i�n� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �v�a�r�i�o�u�s� �c�a�r�o�t�e�n�o�i�d�s�,� �f�a�t�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s�,� �s�t�e�r�o�i�d�s�,� �a�n�d� �f�o�o�d� �a�d�d�i�t�i�v�e�s�.� �V�a�r�i�o�u�s� �o�r�g�a�n�i�c� �s�o�l�v�e�n�t�s� �l�i�k�e� �H�e�x�a�n�e� �H�e�p�t�a�n�e�,� �m�e�t�h�y�l�e�n�e� �c�h�l�o�r�i�d�e� �a�n�d� �a�l�c�o�h�o�l�s� �c�a�n� �b�e� �u�s�e�d�.

�T�h�e� �M�C�I� �G�E�L�T�M� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �a�r�e� �b�a�s�e�d� �o�n� �t�h�e� �s�a�m�e� �c�h�e�m�i�s�t�r�i�e�s� �o�f�f�e�r�e�d� �i�n� �t�h�e� �D�i�a�i�o�n�T�M� �a�n�d� �S�e�p�a�b�e�a�d�s�T�M� �s�y�n�t�h�e�t�i�c� �a�d�s�o�r�b�e�n�t� �r�e�s�i�n�s�.� �T�h�e�s�e� �p�o�l�y�m�e�r� �c�h�e�m�i�s�t�r�i�e�s�,� �l�i�k�e� �D�i�a�i�o�n�T�M� �H�P� �s�e�r�i�e�s� �a�n�d� �S�e�p�a�b�e�a�d�s�™� �S�P� �s�e�r�i�e�s�,� �a�r�e� �w�i�d�e�l�y� �u�s�e�d� �a�n�d� �d�o�c�u�m�e�n�t�e�d� �i�n� �t�h�e� �b�i�o�p�h�a�r�m�a�c�e�u�t�i�c�a�l� �i�n�d�u�s�t�r�y� �f�o�r� �f�e�r�m�e�n�t�a�t�i�o�n� �e�x�t�r�a�c�t�i�o�n�,� �t�h�e� �f�o�o�d� �i�n�d�u�s�t�r�y� �a�n�d� �i�n� �i�n�d�u�s�t�r�i�a�l� �c�h�r�o�m�a�t�o�g�r�a�p�h�i�c� �s�e�p�a�r�a�t�i�o�n�s�.� �T�h�e� �M�C�I� �G�E�L�T�M� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �a�r�e� �a�v�a�i�l�a�b�l�e� �a�s� �p�a�c�k�e�d� �c�o�l�u�m�n�s� �f�o�r� �a�n�a�l�y�t�i�c�a�l� �a�p�p�l�i�c�a�t�i�o�n�s�,� �a�n�d� �a�s� �b�u�l�k� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �f�o�r� �a�n�a�l�y�t�i�c�a�l�,� �p�r�e�p�a�r�a�t�i�v�e� �a�n�d� �p�r�o�d�u�c�t�i�o�n� �c�h�r�o�m�a�t�o�g�r�a�p�h�y� �a�p�p�l�i�c�a�t�i�o�n�s�.

5 4.6×150CHK45/C05

*CHP20/C04, CHP20/C10: USP classification is L21

Br

None

Cation exchange group

None

C18

4

10

4.6×15020×150

4

10

4.6×15020×200

4.6×1504.6×25010×25020×15020×250

4.6×1504.6×25010×15020×15020×250

CHP20/C04

44.6×15020×200CHPOD/C04

CHP20/C10

CHP07/C04

CHP07/C10

4 4.6×150CHK40/C04

4

10

4.6×15020×150

4.6×1504.6×25010×25020×15020×250

CMG20/C04

CMG20/C10

Weak cationexchange group

Column list●CHP column series

5

�M�C�I� �G�E�L�T�M� �C�H�P� �s�e�r�i�e�s� �a�r�e� �s�u�i�t�a�b�l�e� �f�o�r� �R�P� �a�n�d� �N�P� �c�h�r�o�m�a�t�o�g�r�a�p�h�y�.� �T�h�e�r�e� �a�r�e� �f�o�u�r� �k�i�n�d�s� �o�f� �c�o�l�u�m�n�s� �o�f� �v�a�r�i�o�u�s� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s�;� �p�o�r�o�u�s� �p�o�l�y�s�t�y�r�e�n�e�,� �m�o�d�i�f�i�e�d� �p�o�r�o�u�s� �p�o�l�y�s�t�y�r�e�n�e�,� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e�,� �a�n�d� �m�o�d�i�f�i�e�d� �p�o�r�o�u�s� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e�.� �T�h�i�s� �r�a�n�g�e� �o�f� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �o�f�f�e�r�s� �t�r�e�m�e�n�d�o�u�s� �s�c�o�p�e� �f�o�r� �a� �p�r�o�p�e�r� �s�e�l�e�c�t�i�o�n� �o�f� �c�o�l�u�m�n�s� �b�a�s�e�d� �o�n� �t�h�e� �p�r�o�p�e�r�t�i�e�s� �o�f� �t�h�e� �t�a�r�g�e�t� �c�o�m�p�o�u�n�d�s�.

Full range

Column size[mm I.D.×mm]

Particle size[μm] pH range

�P�o�l�y�s�t�y�r�e�n�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�2�0�/�C�0�4�,� �C�H�P�2�0�/�C�1�0�M�o�d�i�f�i�e�d� �p�o�l�y�s�t�y�r�e�n�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�0�7�/�C�0�4�,� �C�H�P�0�7�/�C�1�0�,� �C�H�K�4�0�/�C�0�4�P�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�M�G�2�0�/�C�1�0�M�o�d�i�f�i�e�d� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�O�D�/�C�0�4�,� �C�H�K�4�5�/�C�0�5

MCI GEL™ CHP07/C04, C10 > CHP20/C04, C10 > CHPOD/C04 ≥ ODS columns ≥ CMG20/C04, C10�P�o�l�y�m�e�r� �c�o�l�u�m�n�s� �f�o�r� �H�P�L�C�,� �w�i�t�h� �t�h�e�i�r� �s�u�p�e�r�i�o�r� �c�h�e�m�i�c�a�l� �r�e�s�i�s�t�a�n�c�e�,� �c�a�n� �b�e� �u�s�e�d� �w�i�t�h� �v�a�r�i�o�u�s� �m�o�b�i�l�e� �p�h�a�s�e�s� �o�f� �b�r�o�a�d� �p�H� �r�a�n�g�e�,� �a�c�i�d�i�c� �t�h�r�o�u�g�h� �a�l�k�a�l�i�n�e�.� �T�h�e�y� �h�a�v�e� �t�h�e� �f�o�l�l�o�w�i�n�g� �a�d�v�a�n�t�a�g�e�s� �d�u�e� �t�o� �t�h�e�i�r� �h�i�g�h� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s�:�1）�I�n� �r�e�v�e�r�s�e� �p�h�a�s�e� �c�h�r�o�m�a�t�o�g�r�a�p�h�i�c� �m�e�t�h�o�d�s� �t�o� �s�e�p�a�r�a�t�e� �a�c�i�d�i�c� �o�r� �a�l�k�a�l�i�n�e� �c�o�m�p�o�u�n�d�s�,� �e�l�u�e�n�t�s� �t�h�a�t� �c�a�n� �s�u�p�p�r�e�s�s� �t�h�e�

ionic properties of such compounds are generally used. Polymer columns can be applied in these cases where ODS �c�o�l�u�m�n�s� �w�o�u�l�d� �b�e� �u�n�s�u�i�t�a�b�l�e�.

�2）Some extremely hydrophilic compounds, e.g., oligosaccharides, can be separated using strongly hydrophobic �C�H�P�0�7�/�C�0�4� �o�r� �C�H�P�0�7�/�C�1�0� �c�o�l�u�m�n�s�.

�3）� �P�o�l�y�m�e�r� �c�o�l�u�m�n�s� �c�a�n� �b�e� �w�a�s�h�e�d� �w�i�t�h� �a�c�i�d�i�c� �a�n�d�/�o�r� �b�a�s�i�c� �s�o�l�u�t�i�o�n�s� �i�n� �c�a�s�e� �o�f� �c�o�n�t�a�m�i�n�a�t�i�o�n�.�P�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �c�o�l�u�m�n�s�,� �C�M�G�2�0�/�C�0�4� �a�n�d� �C�M�G�2�0�/�C�1�0�,� �c�a�n� �b�e� �a�p�p�l�i�e�d� �b�o�t�h� �f�o�r� �r�e�v�e�r�s�e� �p�h�a�s�e� �a�n�d� �n�o�r�m�a�l� �p�h�a�s�e� �c�h�r�o�m�a�t�o�g�r�a�p�h�y�.Modified polystyrene packing, CHK40/C04, is a mixed-mode type material; both hydrophobic and hydrophilic �i�n�t�e�r�a�c�t�i�o�n�s� �o�c�c�u�r� �b�e�t�w�e�e�n� �t�h�e� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l� �s�u�r�f�a�c�e� �a�n�d� �t�h�e� �a�n�a�l�y�t�e�s�.� �T�h�i�s� �m�a�t�e�r�i�a�l� �i�s� �u�s�e�f�u�l� �f�o�r� �c�o�m�p�o�u�n�d�s� �t�h�a�t� are difficult to separate using existing ODS or other polymer-based columns. This column is also used in the normal �p�h�a�s�e� �m�o�d�e� �a�n�d� �s�h�o�w�s� �a� �u�n�i�q�u�e� �s�e�p�a�r�a�t�i�o�n� �p�r�o�f�i�l�e�.� All polymeric columns exhibit superior stability and yield in comparison to ODS columns, which may have free silanol groups even when end-capping agents have been used.

�T�h�e� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s� �o�f� �t�h�e� �c�o�l�u�m�n�s� �a�r�e� �i�n� �t�h�e� �f�o�l�l�o�w�i�n�g� �o�r�d�e�r�s�:

Product nameMatrix Type Functinal group USP

2～12

L21

Analytical and preparative chromatography columns and materials for pharmaceutical applicationsCHP column series

Styrene Divinylbenzene

Methacrylates

Analytical and preparative chromatography columns and materials for pharmaceutical applications

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MCI GEL™ colum

n listChiral separationcolum

ns

Analytical and preparative chromatography columns and materials for pharm

aceutical applicationsCom

pounds indexMCI GEL™ material list

Bioseparation columns

and materials

Ion chromatography

columns and m

aterialsIon exchange colum

ns and m

aterialsColum

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MCI GEL™ colum


ns





and materials

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columns and m


ns and m

aterials

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guide

MCI GEL™ colum


ns





and materials

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columns and m

aterials

Ion exchange columns

and materials

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MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





and materials

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columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


nsAnalytical and preparative chromatography columns and materials for pharm




and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum

n list

MCI GEL™ colum

n list

Chiral separationcolum

ns





and materials

SPE sorbent seriesIon chrom

atography colum

ns and materials


and materials

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MCI GEL™ colum

n list


ns




MCI GEL™ material list


and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide


ns



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Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series


aceutical applicationsBioseparation colum

ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

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guide

MCI GEL™ colum

n list

SPE sorbent series


nsSPE sorbent series





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

SPE sorbent seriesChiral separationcolum

nsMCI GEL™ material list

MCI GEL™ colum

n listBioseparation colum

ns and m

aterialsIon chrom

atography colum

ns and materials


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aceutical applications


Compounds index

Compounds index

MCI GEL™ colum


ns and m

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ns and materials


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MCI GEL™ colum


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MCI GEL™ colum


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aceutical applicationsMCI GEL™ material list

MCI GEL™ colum


ns and m

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atography colum

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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum








MCI GEL™ colum


ns and m

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Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

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columns and m


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aterialsColum

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カラム選択の手引き

イオン交換クロマトグラフィー用

充填カラム及び充填剤

イオンクロマトグラフィー用


生体高分子分離用


医薬精密精製用充填カラム

及び充填剤

アミノ酸DL分離用

充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧

分離例化合物リスト

7

固相吸着剤

33 34

Durability of polymeric column

Fig. 5-1 Column durability at pH12 comparison between CHP20/C04 and an ODS column

�T�h�e� �p�o�l�y�m�e�r�i�c� �R�P� �c�o�l�u�m�n�s� �a�r�e� �c�h�e�m�i�c�a�l�l�y� �s�t�a�b�l�e�.� �S�p�e�c�i�f�i�c�a�l�l�y�,� �t�h�e� �c�o�l�u�m�n�s� �h�a�v�e� �r�e�s�i�s�t�a�n�c�e� �t�o� �a�n� �a�l�k�a�l�i�n�e� �e�l�u�e�n�t�.� �T�h�e� �f�o�l�l�o�w�i�n�g� �g�r�a�p�h�s� �d�e�m�o�n�s�t�r�a�t�e� �s�t�a�b�i�l�i�t�y� �o�f� �t�h�e� �p�o�l�y�m�e�r�i�c� �c�o�l�u�m�n�s�.� �A�f�t�e�r� �f�e�e�d�i�n�g� �a� �s�o�l�u�t�i�o�n� �o�f� �p�H� �1�2� �i�n�t�o� �t�h�e� �M�C�I� �G�E�L�™� �C�H�P�2�0�/�C�0�4�,� �t�h�e�r�e� �i�s� �n�o� �c�h�a�n�g�e� �o�f� �c�o�l�u�m�n� �p�e�r�f�o�r�m�a�n�c�e�.

100 150 200500

1,2

0.4

0.6

0.2

0.8

1

0

degr

adat

ion

ratio

fiowing time hr.

CHP20/C04

ODS column

100 150 200500

1,2

0.4

0.6

0.2

0.8

1

0

degr

adat

ion

ratio

fiowing time hr.

CHP20/C04

ODS column

：MCI GEL™ CHP20/C04 4.6mmI.D × 150mm：20mM Na2HPO4 pH12/CH3CN/=60/40：0.4：25℃：254nm：1000ppm Dimethyl phthalate 5µL

ConditionsColumn EluentFlow rateColumn temp.DetectionSample

COOH

NH2 H2N-CH2(CH2)2-CH3

Polystyrenic adsorbent Silica gel C4, C18 Polymethacrylic adsorbent

Polymethacrylic adsorbent Silica gel C4, C18

π-π interaction(double bond effect) Alkyl effect

Hydrogen bond effect Hydrogen bond effect

Hydrogen bond effect

HN

NH2

COOHNH

H2N

(CH2)n-CH3O

SiSi

H2N-CH2 HN NH

HN

OHO O

O OCOH

OSi

Si

H2NCOOH

NH2NH

HN

C

O O

O OC

C

Retentiveness in reverse phase mode

Hydrophobic interaction Interaction mechanism

Weak interactionStronge interaction

Hydrophobic interaction

Interaction mechanism

1

2

3

4

5

0 5 10 15 20 25 30 35 40 min.

1

2

3

0 5 10 15 20 25 30 35 40 min.

MeOOC COOMe

Phthalic acid dimethyl ester

EtOOC COOEt

Phthalic acid diethyl ester

PrOOC COOPr

Phthalic acid dipropyl ester

Application data of CHP series

：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：50mM Na-phosphate pH2.0,　1.5% Hexanesulfonic acid / 　CH3CN＝80/20：0.25：ambient：280nm：1. Epinephrine　2. Dopamine　3. 5-Hydroxy tryptophan　4. Serotonin　5. Tryptophan

ConditionsColumn

Eluent

Flow rateColumn temp.DetectionSample

：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：H2O/CH3CN＝50/50：0.75：60℃：254nm：1. Dimethyl phthalate　2. Diethyl phthalate　3. Dipropyl phthalate

ConditionsColumn

EluentFlow rateColumn temp.DetectionSample

ConditionsColumn


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：H2O/CH3CN=10/90：0.4：25℃：275nm ：1.Theophylline　2.Theobromine　3.Caffeine

ConditionsColumn

Eluent


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：0.1M NaH2PO4 pH2.0 CH3CN=88/12：0.3：25℃：275nm ：1.Cinchonine　2.Quinine

Fig. 5-2 Separation of catecholamines Fig. 5-3 Separation of phthalic acid esters

HO

HOOH H

N

Epinephrine

HO

HO NH2

Dopamine

HO

NH

NH2

COOH

5-hydroxy tryptophan

HO

NH

NH2

Serotonin

12

3

0 5 10 15 20 25 min.

1

2

0 5 10 15 20 25 min.

Fig. 5-4 Purine alkaloids Fig. 5-5 Cinchona alkaloids

HN

N N

N

O

O

Theophylline

N

N N

HN

O

O

Theobromine

N

N N

N

O

O

Caffeine

N

HOHH

H

H

N

Cinchonine

N

HOHH

H

H

N

O

Quinine

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MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


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and materials

Ion chromatography

columns and m


ns and m

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guide

MCI GEL™ colum


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and materials

Ion chromatography

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MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

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SPE sorbent series





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columns and m


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MCI GEL™ colum






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MCI GEL™ colum

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MCI GEL™ colum

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ns and materials


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MCI GEL™ colum

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columns and m


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Compounds index



and materials

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MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

SPE sorbent series



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MCI GEL™ colum

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SPE sorbent series







and materials

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columns and m


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MCI GEL™ colum


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Compounds index

MCI GEL™ colum


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atography colum

ns and materials


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guide





MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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ns and materials


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guide

MCI GEL™ colum








MCI GEL™ colum


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ns and materials


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Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



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及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

35 36


Comparison with an ODS column

Fig. 5-6 Uric acid and related compounds

Fig. 5-7 Bile acidsCHP20/C04

ODS column

1

2

3

1

3

2

13

2

4 5

0 5 10 15 20 min.

0 5 10 15 20 min.

0 5 10 15 20 min.

ConditionsColumn


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：10mM TBA/CH3CN=75/25：0.4：25℃：284nm ：1.Hypoxanthine　2.Xanthine　3.Theophylline　4.Uric acid　5.Orotic acid

ConditionsColumn

Eluent


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：50mM NaH2PO4 pH2.0 /CH3CN＝40/60：0.3：25℃：210nm：1.Cholic acid　2.Ursodeoxycholic acid　3.Deoxycholic acid

ConditionsColumn

Eluent


：ODS column (5μm)　4.6mm I.D.×150mm：50mM NaH2PO4 pH6.0 /CH3CN＝40/60：0.3：25℃：210nm：1.Cholic acid　2.Ursodeoxycholic acid　3.Deoxycholic acid

N

N NH

HN

O

Hypoxanthine

HN

N N

N

O

O

Theophylline

HN

NH

N

HN

O

O

Xanthine

NH

HN

COOH

O

Orotic acid

O

HN

NH

N

HN

O

O

HO

Uric acid

H

OHH

COOH

HO

Ursodesoxycholic acid

H

OHH

COOH

HO

cholic acid

OH

H

H

COOH

HO

deoxycholic acid

OH


Fig. 5-9 Adrenal cortex hormones

Fig. 5-8 Glycyrrhizae radix

min.

min.

Glycyrrhizic acid

0 30 35 4020 2515105

2

3

1

0 3020 2515105

ConditionsColumn

Eluent


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：2.06% acetic acid/CH3CN =63/37：0.5：45℃：254nm：Extract of　glycyrrhizae radix

ConditionsColumn

Eluent


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：H2O/CH3CN：60/40：0.5：45℃：280nm：1.Hydrocortisone　2.Corticosterone　3.11-Deoxycortisol

Glycyrrhizic acid

HO

HO

H

H

O

H

OH

OOH

HOH

H

HO H

O

H

OHO

HOH

HOH H

HO HHO

O

OHO

HOOH

O

H

H H

O

HOOH

O

H

H H

OHO

OH

O

H

H H

hydrocortisone corticosterone 11-deoxycortisol

2

3

4

5

6

8

9

10

1

3

4

5

6

8

9

10

1

2

4

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1

2

3

5

6

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9

10

1

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8

9

10

1

2

3

4

5

8 8

9

10

1

2

3

4

5

6

9 9

10

1

2

3

4

5

6

10 10

1

2

3

4

5

6

8

1

2

3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

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Column selection

guide

MCI GEL™ colum


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and materials

Ion chromatography

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and materials

Column selection

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MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

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MCI GEL™ colum






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Ion chromatography

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MCI GEL™ colum

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MCI GEL™ colum

n list


ns





and materials


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ns and materials


and materials

Column selection

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MCI GEL™ colum

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Ion chromatography

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n selectionguide


ns



pounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series



ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum

n list

SPE sorbent series







and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





Compounds index

Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


ns



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide






MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide




Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

4

5

6

7

8

9

1

3

4

5

6

7

8

9

10 10

1

2

4

5

6

7

8

9

1

2

3

5

6

7

8

9

1

2

3

4

6

7

8

9

1

2

3

4

5

7

8

9

1

2

3

4

5

6

8

9

1

2

3

4

5

6

7

9 9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

37 38

Comparison with an ODS column

Fig. 5-10 Ingredients of medicine

Fig. 5-11 Sulfa drugs

1

2

3

4

0 3020 2515105 min.

1

2

34

0 3020 2515105 min.


ConditionsColumn

Eluent


：MCI GEL™ CMG20/C04　4.6mm I.D.×150mm

：20mM phosphate pH6.8/CH3CN　＝82/18：0.5：45℃：254nm：1.Succinylsulfathiazole　2.Sulfanilamide　3.Sulfathiazole　4.Sulfamerazine

ConditionsColumn

Eluent


：MCI GEL™ CMG20/C04　4.6mm I.D.×150mm：50mM phosphoric acid(pH2.0)/CH3OH　＝60/40：0.5：45℃：254nm：1.4-Dimethylaminoantipyrine　2.Antipyrine　3.Caffeine　4.Aspirin　5.Phenacetin

ConditionsColumn

Eluent


：ODS column　4.6mm I.D.×150mm

：20mM phosphate pH6.8/CH3CN　＝90/10：0.5：45℃：254nm：1.Succinylsulfathiazole　2.Sulfanilamide　3.Sulfathiazole　4.Sulfamerazine

min.

1

2

3

4

5

0 3020 2515105

NN

O

Antipyrine

NN

O

DimethylaminoantipyrineAminopyrine

N

COOH

OAc

Aspirin

NHAc

O

Phenacetin

N

N N

N

O

O

Caffeine

S

HN

OO

N

N

NH2

Sulfamerazine

S

HN

OO

N

S

HN

OOH

O

Succinylsulphathiazole

S

HN

OO

N

S

NH2

sulphathiazole

SH2N

OO

NH2

Sulfanilamide


0 108642 min.min.

2

1

3

4

Fig. 5-15 Water-soluble vitaminsFig. 5-14 Procainamide, Procaine

1

2

0 252015105

O

ON

H2N

Procaine

NH

ON

H2N

Procainamide

NH2

O

N

Vitamin B3

C-NCo3+

N

N-

N

N

N

NO

H2N

O

H2N

O

NH2O

OH

H2N

O

OHN

NH2

O

NH2

O

OP

HO

O-OO

Vitamin B12

NH

NHOOH

N

HO

OS

ONH

O

NH2HO

ONH

O

NH2HO

OH

O

NHN

N

O

NH

ONH

HN

O

HO

O

NH

O

NHOH

ONH

O

NH2

NH

HO

O

NH

H2N

H

0 108642 min.

2

1

3 4

0 40302010 min.

2

1

3

HN

HN O

OHN

HO O

ONH

O

NH2HO

N

OHHO

HO

Vitamin B6

OHOHO

OHHO

OH

Vitamin C

Gly-Tyr

Met Enkephalin

Leu Enkephalin

Angiotensin ll

Fig. 5-12 Peptides Fig. 5-13 Proteins

ConditionsColumn

Eluent



：8mM Na2HPO4 pH7.0/CH3CN　＝85/15：0.5：25℃：254nm：1.Vitamin C　2.Vitamin B6　3.Vitamin B3　4.Vitamin B12

ConditionsColumn

Eluent



：20mM phosphate pH7.2/CH3CN　＝65/35：0.5：45℃：254nm：1.Procainamide　2.Procaine

ConditionsColumn

Eluent



：0.1%TFA/CH3CN　=70/30：0.5：25℃：220nm ：1.Gly-Tyr　2.Met Enkephalin　3.Leu Enkephalin　4.Angiotensin ll

ConditionsColumn

Eluent


：MCI GEL™ CMG20/C04　4.6mm I.D.×150mm：A;0.05%TFA/CH3CN =80/20 B;0.05%TFA/CH3CN =20/80　A→B 30min.linear：0.5：25℃：280nm：1.Ribonuclease A　2.Cytochrome c　3.α-Chymotrypsinogen A

2

3

4

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8

9

10

1

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1

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5

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1

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3

4

6

8

9

10

1

2

3

4

5

8 8

9

10

1

2

3

4

5

6

9 9

10

1

2

3

4

5

6

10 10

1

2

3

4

5

6

8

1

2

3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterials

Column selection

guide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m

aterials


and materials

Column selection

guide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum






and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum

n list

MCI GEL™ colum

n list


ns





and materials


atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum

n list


ns






and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide


ns



pounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series



ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum

n list

SPE sorbent series







and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





Compounds index

Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


ns



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide






MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide




Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

4

5

6

7

8

9

1

3

4

5

6

7

8

9

10 10

1

2

4

5

6

7

8

9

1

2

3

5

6

7

8

9

1

2

3

4

6

7

8

9

1

2

3

4

5

7

8

9

1

2

3

4

5

6

8

9

1

2

3

4

5

6

7

9 9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

39 40


Pravastatin sodiumSimvastatin

MevastatinMevastatin

Impurity Impurity

SimvastatinPravastatin sodium

Nystatin

Amphotericin BAmphotericin B

Nystatin

Filipin

Filipin

OHO OH

O

HO

ONa

OH

Pravastatin sodium

O

O O

OHO

H

(+)-Simvastatin, Simvastatin

O

O

OHO

O

H

(+)-Compactin; Mevastatin

Fig. 5-16 Pravastatin sodium

CHP20/C10 10μm ODS 10μm

min.0 5 10 15 20 25 min.0 5 10 15 20 25

min.0 5 10 15 min.0 5 10 15


Fig. 5-17 Polyene antibiotics

UV

238

nm (A

U)

UV

238

nm (A

U)

：MCI GEL™ CHP20/C10 (10μm 250 ×4.6mm I.D.) and　ODS (10μm 250 ×4.6mm I.D.) ： A :0.1% Formic acid; B :0.1% Formic acid in AcCN; A/B=60/40;：1.00：25℃：UV305nm for Nystatin, VIS405nm for Amphotericin B and UV340nm for Filipin; ：Pravastatin sodium, Mevastatin and Simvastatin, 1mg/ml each;. ：10μl

ConditionsColumn

Eluent

Flow rateColumn temp.Detection

Sample

Injection

：MCI GEL™ CHP20/C10 (10μm 250 ×4.6mm I.D.) and　ODS (10μm 250 ×4.6mm I.D.) ： A :0.1% Formic acid; B :0.1% Formic acid in AcCN; ： 45%B-95%B over 29min.：1.00：25℃：UV238nm ：Pravastatin sodium, Mevastatin and Simvastatin, 1mg/ml each;.：5μl

ConditionsColumn

Eluent

GradientFlow rateColumn temp.DetectionSampleInjection

OHOH

O

O

OH

OH

OH OH OH OH

OH

H

Filipin

O

O

O

OH OH OH OH

OH

OHOH

OH

O OH

O

O

OHH2N

HO

Nystatin

O

O

OHH2N

HO

HO

HO

OHO O OH OHOH OH OH

OH

O O

Amphotericin B

min.0

4

3

2

1

10 20 30

5



Fig. 5-19 Proteins

Ribonuclease A Conalbumin Ribonuclease A Conalbumin

ConditionsColumn Eluent

Flow rateColumn temp.DetectionSampleInjection

：150 ×4.6mm I.D. ： A :0.1% TFA; B :0.1% TFA in AcCN：1.00：20%B-60%B over 20min;：UV280nm; ：Ribonuclease A and Conalbumin 2mg/ml;. ：10μl

Fig. 5-18 Proteins

：MCI GEL™ CMG20/C10　4.6mm I.D.×250mm：A 0.05% TFA/CH3CN＝80/20　B 0.05% TFA/CH3CN＝30/70　A → B 45min linear gradient：0.5：25℃：280nm：1. Ribonuclease A　2. Cytochrome C　3. Transferrin　4. α-Chymotrypshinogen A　5. β-Lactoglobulin

ConditionsColumn

Eluent


min. min.0 105 15 20 25 0 105 15 20 25

2

3

4

5

6

8

9

10

1

3

4

5

6

8

9

10

1

2

4

5

6

8

9

10

1

2

3

5

6

8

9

10

1

2

3

4

6

8

9

10

1

2

3

4

5

8 8

9

10

1

2

3

4

5

6

9 9

10

1

2

3

4

5

6

10 10

1

2

3

4

5

6

8

1

2

3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterials

Column selection

guide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m

aterials


and materials

Column selection

guide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum






and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum

n list

MCI GEL™ colum

n list


ns





and materials


atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum

n list


ns






and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide


ns



pounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series



ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum

n list

SPE sorbent series







and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





Compounds index

Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


ns



MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide






MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide




Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

4

5

6

7

8

9

1

3

4

5

6

7

8

9

10 10

1

2

4

5

6

7

8

9

1

2

3

5

6

7

8

9

1

2

3

4

6

7

8

9

1

2

3

4

5

7

8

9

1

2

3

4

5

6

8

9

1

2

3

4

5

6

7

9 9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

41 42

ConditionsColumn

Eluent


：　

：：：：：：：

MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmA) 0.1%TFA, H2OB) 0.1%TFA, AcCN10%B→60%B over 25min.1.040℃230nmGhrelin rat and Ghrelin human ,0.1mmol/l each10μl

Fig. 5-21 Ghrelin


CMG20/C10 10μm ODS 10μm

CHP20/C10 10μm

ODS 10μmCMG20/C10 10μm

Insulin glargine

UV2

30nm

(AU

)U

V230

nm(A

U)

UV2

30nm

(AU

)

UV2

30nm

(AU

)

UV2

30nm

(AU

)

Insulin human recombinant

Insulin glargine Insulin human recombinantInsulin glargine Insulin human recombinant

Ghrelin rat Ghrelin humanGhrelin rat

Ghrelin human

0 5 10 15 20min.

0 5 10 15 20min.

0 2 4 6 8 10 1412 1816 20min.

0 2 4 6 8 10 1412 1816 20min.

0 5 10 15 20min.

ConditionsColumn

Eluent


：MCI GEL™ CHP20/C10 MCI GEL™ CMG20/C10 ODS 10μm 4.6mm I.D.×150mm：A) 0.1%TFA, H2O B) 0.1%TFA, CH3OH：20%B→60%B over 20min.：1.0：40℃：280nm：Insulin Glargine and human recombinant , 1mg/ml each：10μl

Fig. 5-20 Insulin


CMG20/C10 10μm ODS 10μm

TargetU

V210

nm(A

U)

Impurity

Target

Impurity

0 5 10 15 20 25 30 35 40min.

UV2

10nm

(AU

)

0 5 10 15 20 25 30 35 40min.

ConditionsColumn

EluentFlow rateColumn temp.DetectionSampleInjection

：　

：：：：：：

MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mm0.1%TFA,/CH3CN=68/321.0ml/min40℃210nmSifuvirtide crude(purity 35.5%) 2.1mg/ml0.4ml

Fig. 5-23 Sifuvirtide

Fig. 5-22 Leuprorelin

ConditionsColumn

Eluent


MCI GEL™ CHP20/C10MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmA) 0.1%TFA, H2OB) 0.1%TFA, AcCN20%B→60%B over 20min.1.040℃280nmLeuprorelin,LHRH human, LHRH salmon and Buserelin ,1mg/ml each10μl

：　

：：：：：：：

0 2 4 6 8 10 12 14 min. 0 2 4 6 8 10 12 14 min.

Leuprorelin LeuprorelinBuserelin

Buserelin

Leuprorelin Buserelin

LH-RH salmon LH-RH salmon

LH-RH human

LH-RH human

LH-RH salmon

LH-RH human

CMG20/C10 10μm

CHP20/C10 10μm

UV2

30nm

(AU

)

ODS 10μm

UV2

30nm

(AU

)

0 2 4 6 8 10 12 14 min.

UV2

30nm

(AU

)

2

3

4

5

6

8

9

10

1

3

4

5

6

8

9

10

1

2

4

5

6

8

9

10

1

2

3

5

6

8

9

10

1

2

3

4

6

8

9

10

1

2

3

4

5

8 8

9

10

1

2

3

4

5

6

9 9

10

1

2

3

4

5

6

10 10

1

2

3

4

5

6

8

1

2

3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterials

Column selection

guide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m

aterials


and materials

Column selection

guide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





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Ion chromatography

columns and m


ns and m

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n selectionguide

MCI GEL™ colum






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Ion chromatography

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ns and m

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n selectionguide

MCI GEL™ colum

n list

MCI GEL™ colum

n list


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and materials


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ns and materials


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Column selection

guide

MCI GEL™ colum

n list


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Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide


ns



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Compounds index



and materials

Ion chromatography

columns and m


ns and m

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n selectionguide

MCI GEL™ colum


ns

SPE sorbent series

SPE sorbent series

SPE sorbent series



ns and m

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atography colum

ns and materials


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Column selection

guide

MCI GEL™ colum

n list

SPE sorbent series







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Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



MCI GEL™ colum


ns and m

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guide





Compounds index

Compounds index

MCI GEL™ colum


ns and m

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atography colum

ns and materials


and materials

Column selection

guide





MCI GEL™ colum


ns and m

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Ion chromatography

columns and m


ns and m

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n selectionguide





MCI GEL™ colum


ns and m

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ns and materials


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MCI GEL™ colum


ns and m

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atography colum

ns and materials


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MCI GEL™ colum


ns and m

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atography colum

ns and materials


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MCI GEL™ colum


ns and m

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atography colum

ns and materials


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Column selection

guide






MCI GEL™ colum


ns and m

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atography colum

ns and materials


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Column selection

guide




Compounds index

MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

aterialsIon chrom

atography colum

ns and materials


and materials

Column selection

guide


Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

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9 9

1

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8

9

1

2

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5

6

7

8

1

2

3

4

5

6

7

8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

43 44


Fig. 5-25 Nucleotide

Fig. 5-24 ssRNA Ladder Marker

ConditionsColumn

Eluent

Gradient


：MCI GEL™ CMG20/C10 ODS 10μm 4.6mm I.D.×150mm：A)100mM TEAA, H2O B)100mM TEAA, CH3CN：CHP10/C10 ODS 10μm：1.0：40℃：260nm：14-30 ssRNA Ladder Marker [max.0.04mg/ml]：5μl

10%B→40%B over 30min8%B→40%B over 30min

0-4.0min 0%B　　4.0-5.0min 0→30%B　 5.0min-6.0min 30%B　 6.0min-7.0min 30→50%B7.0min-10.0min 50→65%B　　 10.0min-11.0min 65%B　11.0min-　　 0%B

1.Ura，2.Xan，3.Thy，4.Hyp，5.Gua，6.Cyt，7.Ade, 8.Urd，9.Xao，10.dT，11.Ino，12.Guo，13.Cyd，14.Ado

B)20 mM Na2HPO4 / 100 mM NaClO4 / 30% ACN

ConditionsColumn

Eluent

Gradient


：MCI GEL™ CHK40/C04 4.6mm I.D.×150mm：A)19 mM H3PO4 / 1 mM NaH2PO4 / 5.0% ACN ：：0.8：50℃：UV260nm：：20μl

tR / mim

inte

nsity

/ -

Ura

Urd

Xan

Xao

Ino Th

y

dT

Hyp

Guo

Gua

Ade

Cyd

Cyt

Ado

0 2 4 6 8 10 12 14

0 5 10 15min.

ODS 10μm 40℃

CHP20/C10 40℃

CHP20/C10 60℃

UV

260n

m(A

U)

18 base

14 base22 base

26 base30 base

(Data provided by Professor Yokoyama of Yokohama National University)


LinaloolUV2

10nm

(AU

)

0 2 864 10分

CMG20/C10 10μm

ConditionsColumn


Injection

：

：：：：：

：

Fig. 5-26 Linalool

0 5 10 15 20min.

CMG20/C10 10μm

UV2

10nm

(AU

) 12

3

4 5

0 5 10 15 20min.

ODS 10μm

UV2

10nm

(AU

)

1

2

3

4

5

ConditionsColumn

EluentFlow rateColumn temp.DetectionSampleInjection

：MCI GEL™ CMG20/C10 4.6mm I.D.×150mm：Hexan/Ethanol=99.5/0.5：1.0：40℃：210nm：Coriander：10μl

MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmHexan/Ethanol=99.5/0.51.040℃210nm1:Linalyl Acrtate 1mg/ml2:Linalool 1mg/ml3:β-Citronellool 1mg/ml4:Nerol 0.5mg/ml5:Geraniol 0.5mg/ml10μl

Fig. 5-27 Coriander

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1

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8

1

2

3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide

MCI GEL™ colum


ns





and materials

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columns and m


ns and m

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Column selection

guide

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m

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and materials

Column selection

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MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





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ns and m

aterialsColum

n selectionguide

MCI GEL™ colum






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MCI GEL™ colum

n list


ns





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ns and materials


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MCI GEL™ colum

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ns






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columns and m


ns and m

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ns



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Compounds index



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ns and m

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n selectionguide

MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

SPE sorbent series



ns and m

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MCI GEL™ colum

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SPE sorbent series







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MCI GEL™ colum


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Compounds index

MCI GEL™ colum


ns and m

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atography colum

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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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atography colum

ns and materials


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MCI GEL™ colum


ns and m

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atography colum

ns and materials


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Compounds index

MCI GEL™ colum


ns and m

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atography colum

ns and materials


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guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

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atography colum

ns and materials


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Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

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9

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1

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9 9

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8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

45 46


(TritonX-100)

min. min.

Fig. 5-32 ODS-1HU (ODS)Fig. 5-31 C18-alkylated aliphatics CHPOD/C04

ConditionsColumn


：MCI GEL™ CHPOD/C04　4.6mm I.D.×150mm：50vol%CH3CN：0.50：40℃：254nm：Triton X-100 (Polyoxyethylene octyl phenyl ether) 1% each 10μL

ConditionsColumn


：MCI GEL™ ODS-1HU　4.6mm I.D.×250mm：50vol%CH3CN：1.00：40℃：254nm：Triton X-100 (Polyoxyethylene octyl phenyl ether) 1% each 10μL

n=9.6 n=9.6

Fig. 5-33　Application data of Nucleic base/Nucleoside and Beer

0-0.7min 0%B 0.7-3.0min 0→40%B 3.0-3.2min 40%B3.2-3.5min 40→80%B　3.5-8.0min 80%Β 8.0min- 0%B

ConditionsColumn

Eluent

Gradient

Flow rateColumn tempDetectionInjection

：MCI GEL™ CHK45/C05　4.6mm I.D.×150mm：A）8 mM H3PO4

B）10 mM H3PO4 /30% ACN

：1.3：45℃：UV(260nm)：20μl

1211109876543210

1211109876543210

1211

Draft Beer

Carbohydrate-free "happoshu-type" beer

A new category of beer that contains 85% less purine

109876543210

Analysis of various category beer

Urd

Ura

dT

Cyd

Urd

Urd

Ura

dT Cyd

Ino

Xan

Guo

Gua

dA Ade

Ino

Ura Ino

Xan

dT Xan

Guo

Guo

Gua

Gua

dA

Ade

0

Inte

nsity

/-

Ino

Ura

Xan

Hyp

Xao

Urd

AMP

UM

P

Cvd

Guo

dT

GD

P

Cvt

dCG

ua

dA

Ade

Ado

tR / min2 4 6 8 10 12



(Polyphenon 60)Fig. 5-30 Styrene Divinylbenzene CHP20/C04Fig. 5-29 Modified Styrene Divinylbenzene

CHP07/C04ConditionsColumn


：MCI GEL™ CHP20/C04　4.6mm I.D.×150mm：CH3OH/10mM-Acetic acid＝60/40：0.46：60℃：280nm：Polyphenon 60(10mg/ ) each 10μL

ConditionsColumn


：MCI GEL™ CHP07/C04　4.6mm I.D.×150mm：CH3OH/10mM-Acetic acid＝60/40：0.46：60℃：280nm：Polyphenon 60(10mg/ ) each 10μL


Fig. 5-28 Application data of CHK40/C04：Sepatation of Sartans

N

O

HO

O

NH

N

NN

Valsartan

N

ON

HO O

NHNNN

Candesartan

O

N

N

HN NN N

Irbesartan

NN

HO

Cl NHNNN

Losartan

ConditionsColumn

Eluent

Gradient


Injection

：MCI GEL™ CHK40/C04 4.6mm I.D.×150mm：：

：1.0：50℃：UV：Candesartan(CAN),Losartan(LOS), Valsartan(VAL), Irbesartan(IRB) ：20μl

A）10 mM Na H↓ 2 PO↓ 4 +0.2 mM Na↓ 2 HPO↓ 4 (25%ACN)B）10 mM Na H↓ 2 PO↓ 4 +1.0 mM Na↓ 2 HPO↓ 4 (40%ACN)0.5min 0%B 0.5-2.0min 50%B2.0min- 90%Β

min.min.

min.642

CAN

LOS

VAL

IRB

0

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10

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1

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3

4

5

6

8

7 7 7 7 7 7 7 7 7

9

1

2

3

4

5

6

8

7

9

10

MCI GEL™ colum


ns





and materials

Ion chromatography

columns and m


ns and m

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MCI GEL™ colum


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columns and m


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guide

MCI GEL™ colum


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and materials

Ion chromatography

columns and m

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SPE sorbent series

SPE sorbent series

SPE sorbent series

SPE sorbent series





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columns and m


ns and m

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MCI GEL™ colum






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MCI GEL™ colum

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MCI GEL™ colum

n list


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ns and materials


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Column selection

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MCI GEL™ colum

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ns






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Ion chromatography

columns and m


ns and m

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Compounds index



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columns and m


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MCI GEL™ colum


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SPE sorbent series

SPE sorbent series

SPE sorbent series



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MCI GEL™ colum

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SPE sorbent series







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Compounds index

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atography colum

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guide





MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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MCI GEL™ colum


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guide






MCI GEL™ colum


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atography colum

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guide




Compounds index

MCI GEL™ colum


ns and m

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atography colum

ns and materials


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Column selection

guide

MCI GEL™ colum








MCI GEL™ colum


ns and m

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atography colum

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Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index

Compounds index



and materials

Ion chromatography

columns and m


ns and m

aterialsColum

n selectionguide



2

3

4

5

6

7

8

9

1

3

4

5

6

7

8

9

10 10

1

2

4

5

6

7

8

9

1

2

3

5

6

7

8

9

1

2

3

4

6

7

8

9

1

2

3

4

5

7

8

9

1

2

3

4

5

6

8

9

1

2

3

4

5

6

7

9 9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

1

2

3

4

5

6

7

8

1

2

3

4

5

6

7

8

9

10 10 10 10 10 10 10 10

1

2

3

4

5

6

9

10

11









及び充填剤


充填カラム

MCI

GEL

カラム一覧

MCI

GEL

充填剤一覧


7

固相吸着剤

47 48

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