chromatin immunoprecipitation of site-specific tissue - magna chip g tissue kit
DESCRIPTION
Tissue-specific ChIP (chromatin immunoprecipitation) is a powerful method of elucidating mechanisms by which cell microenvironment can affect gene regulation. Conventional chromatin immunoprecipitation (ChIP) protocols, including our popular Magna ChIP and EZ Magna ChIP assay kits, work best using homogenous populations of cultured cells. In contrast, the Magna ChIP G Tissue Kit is specifically designed for tissue samples, which are more scientifically reliant, but also more complex, heterogeneous mixtures of cells that are less amenable to ChIP.TRANSCRIPT
ChIP your precious tissue with a single punch.
EMD Millipore is a division of Merck KGaA, Darmstadt, Germany
Region Specific Tissue Isolation. A 300 µm coronal mouse brain cryosection was obtained and two microdissections were carried out using the 1 mm microdissection punch provided in the kit. The isolated tissue is shown placed above the dissected region: (A) hippocampus, (B) cortex.
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Magna ChIP™ G Tissue Kit (Catalogue No. 17-20000)
For reproducible and reliable chromatin immunoprecipitation (ChIP) of site-specific tissue biopsies, count on the Magna ChIP G Tissue Kit.
Tissue-specific ChIP is a powerful method of elucidating
mechanisms by which cell microenvironment can affect gene
regulation. Conventional chromatin immunoprecipitation (ChIP)
protocols, including our popular Magna ChIP and EZ Magna ChIP
assay kits, work best using homogenous populations of cultured
cells. In contrast, the Magna ChIP G Tissue Kit is specifically
designed for tissue samples, which are more scientifically reliant,
but also more complex, heterogeneous mixtures of cells that are
less amenable to ChIP.
Magna ChIP G Tissue Kit Includes:
• Tools necessary to microdissect tissue sections
that are < 1 mm3 in size
• Tissue stabilization and lysis reagents
• Optimized reagents and protocols for chromatin
immunoprecipitation and DNA purification
The M logo is a trademark of Merck KGaA, Darmstadt, Germany. Magna ChIP is a trademark of Millipore Corporation.Lit No. PB3176EN00 LS-SBU-11-04193 Printed in the USA. 3/2011 © 2011 Millipore Corporation. All rights reserved.
www.emdmillipore.com
Description Quantity Catalogue No.
Magna ChIP™ G Tissue Kit 1 kit 17-20000
Related Products:ChIPAb+ ChIP-Validated Antibody/Primer Sets
Description Catalogue No.
ChIPAb+ Phospho-Histone H3 (Ser10) 17-685
ChIPAb+ Trimethyl-Histone H3 (Lys4) 17-678
ChIPAb+ Acetyl-Histone H4 (Lys5) 17-10045
ChIPAb+ Trimethyl-Histone H3 (Lys9) 17-625
ChIPAb+ Trimethyl-Histone H3 (Lys27) 17-622
ChIPAb+ Acetyl-Histone H3 (Lys14) 17-10051
ChIPAb+ Dimethyl-Histone H3 (Lys27) 17-10108
ChIPAb+ Monomethyl-Histone H3 (Lys9) 17-680
ORDeRING INfORMATION
CONTACT USIn the U.S. and Canada, call toll-free 1 800-Millipore (1-800-645-5476).
For Technical Service, please visit www.millipore.com/techservice.
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Pol II / GAPDH Pol II / UpST (-)
Antibody Target/Primer Set Antibody Target/Primer Set
Antibody Target/Primer Set Antibody Target/Primer Set
Sp1 / Dhfr Sp1/ UpST (-)0
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Antibody Target/Primer Set Antibody Target/Primer Set
Sp1 / Dhfr Sp1/ UpST (-)0
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CEREBRAL CORTEX
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Antibody Target/Primer Set Antibody Target/Primer Set
Antibody Target/Primer Set Antibody Target/Primer Set
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Antibody Target/Primer Set Antibody Target/Primer Set
Antibody Target/Primer Set Antibody Target/Primer Set
Sp1 / Dhfr Sp1/ UpST (-)0
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CEREBRAL CORTEX
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Pol II / GAPDH Pol II / UpST (-) Sp1 / Dhfr Sp1/ UpST (-)0
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KIDNEY
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Pol II / GAPDH Pol II / UpST (-) Sp1 / Dhfr Sp1/ UpST (-)0
Chromatin from various mouse tissues was subjected to immunopre-cipitation with Anti-RNA Polymerase II, clone CTD4H8 (Catalogue No. 05-623B) and Anti-SP1 (Catalogue No. 07-645) using the Magna ChIP G Tissue kit. The IgG used for relative comparison was either Rabbit IgG (Catalogue No. 12-370) or Mouse IgG (Catalogue No. 12-371). Quantitative PCR was performed using primers specific for either the GAPDH promoter, Dhfr promoter, or an upstream region (negative con-trol). Data above is presented as fold relative enrichment to IgG.
Anti-RNA Polymerase II, clone CTD4H8 (Catalogue No. 05-623): 1 µg of mouse monoclonal affinity purified antibody immunoprecipitated with chromatin of various mouse tissue and qPCR assayed with primers specific for mouse GAPDH promoter: Forward: 5’ CAC CAT CCG GGT TCC TAT AAA TAC 3’, Reverse: 5’ CAG CAT CCC TAG ACC CGT ACA 3’
Anti-SP1 (Catalogue No. 07-645): 1 µg of mouse monoclonal affinity purified antibody immunoprecipitated from chromatin of various mouse tissue and qPCR assayed with primers specific for mouse Dhfr promoter: Forward: 5’ CAC GCC TCA ACC TGT GCG GGA 3’, Reverse: 5’ GCG GGG ATA AAA TCC TAC CAG CC 3’.
Tissue Targeted ChIP experiments using Magna ChIP G Tissue Kit
FSC Label – Landscape Artwork Matrix
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FSC Label Artwork
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FSC_MS_3_LPC.EPSFSC_MS_3_LPC.JPGFSC_MS_3_LPC.TIF
FSC_MS_4_LPC.EPSFSC_MS_4_LPC.JPGFSC_MS_4_LPC.TIF
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FSC_Labels_English
FSC_Labels_LPC
FSC_Labels_Landscape
Landscape / Positive / Colour (LPC)
FSC_RE_1_LNC.EPSFSC_RE_1_LNC.JPGFSC_RE_1_LNC.TIF
FSC_MS_1_LNC.EPSFSC_MS_1_LNC.JPGFSC_MS_1_LNC.TIF
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FSC_100_LNC.EPSFSC_100_LNC.JPGFSC_100_LNC.TIF
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FSC_Labels_LNCLandscape / Negative / Colour (LNC)
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FSC_MS_1_LPBW.EPSFSC_MS_1_LPBW.JPGFSC_MS_1_LPBW.TIF
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FSC_Labels_LPBWLandscape / Positive / Black & White (LPBW)
FSC_RE_1_LNBW.EPSFSC_RE_1_LNBW.JPGFSC_RE_1_LNBW.TIF
FSC_MS_1_LNBW.EPSFSC_MS_1_LNBW.JPGFSC_MS_1_LNBW.TIF
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FSC_100_LNBW.EPSFSC_100_LNBW.JPGFSC_100_LNBW.TIF
Cert no. SCS-COC-001623
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