chromatograph - ftp.mn-net.comftp.mn-net.com/english/flyer_catalogs/...18 pyramid, 3 μm 1 760261.20...

CHROMATOGRAPHY

Think about foodCare about safety

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot DeutschlandFrankreichMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-Mail sales-frmn-netcom

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Deutschlandund InternationalTel +49 24 21 969-0Fax +49 24 21 969-199E-Mail infomn-netcom

MACHEREY-NAGEL

EN ISO 9001 2008ZERTIFIZIERT

USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-Mail sales-usmn-netcom

Vol 6Mycotoxins

infomn-netcom wwwmn-netcom2 3

Focus on mycotoxins The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 6 Mycotoxins in food infested with moldMycotoxins are secondary metabolites of fungi molds In contrast to environmental contaminants (pesticides PCBs PAHs etc) mycotoxins are inevitable More than 300 mycotoxins are chemically characterized [Food and Agriculture Organization of the United Nations (FAO) ndash httpwwwfaoorgdocrep005y1390ey1390e0bhtm] The follow-ing mycotoxins can be considered as the most common mycotoxins in feed and food

Aflatoxins (aflatoxin B1 B2 G1 and G2 aflatoxin M1) ochratoxin A (OTA) trichothecenes (Deoxynivalenol ndash

DON nivalenol T-2 toxin HT-2 toxin) patulin ergot al-kaloids zearalenone ndash ZEA fumonisins B1 and B2)

The presence of mycotoxins in food and feed may affect the health of humans and animals Many adverse health effects are suspected such as causing of cancer and mutations and excess estrogens gastrointestinal and kidney disorders [Food and Agriculture Organization of the United Nations (FAO) ndash httpwwwfaoorgwairdocsx5008ex5008e01htm]

Toxicological properties of selected mycotoxinsMycotoxin Possible effects

Aflatoxins Liver diseases (hepatotoxic hepatocarcinogenic) carcinogenic and teratogenic effects hemorrhages (intestinal tract kidneys) reduced growth rate diminution of performance immune suppression

Ochratoxins Nephrotoxic carcinogenic mild liver damage enteritis teratogenic effects poor feed conversion reduced growth rate immune suppression

Fumonisins Pulmonary edema equine leukoencephalomalacia nephro- and hepatotoxic immune suppression

Trichothecenes Digestive disorders (vomiting diarrhea feed refusal) reduced weight gain hemorrhages (stomach heart intestine lung bladder kidney) edema oral lesions dermatitis blood disorders infertility degeneration of bone marrow slow growth immune suppression

Zearalenone Estrogenic effects edema of vulva prolapse of vagina enlargement of uterus atrophy of testicles atrophy of ovaries enlargement of mammary glands infertility abortion

Ergot alkaloids Nervous or gangrenous syndromes Digestive disorders (vomiting diarrhea feed refusal) reduced weight gain convulsion abortion

Citrinin Nephrotoxic teratogenic hepatotoxic

Patulin Mutagenic genotoxic neurotoxic immune suppression

Richard J L (2007) Some major mycotoxins and their mycotoxicoses ndash an overview International Journal of Food Microbiology 119 (1ndash2) 3ndash10


Compounds of interestChemical structures of selected mycotoxins

Aflatoxin B1 Aflatoxin B2 Aflatoxin M1

OCH3

O

O

OO

O

OCH3

O

O

OO

O

OCH3

O

O

OO

O

OH

Aflatoxin G1 Aflatoxin G2 Aflatoxin M2

OCH3

O O

O O

OO OCH3

O O

O O

OO OCH3

O

O

OO

O

OH

Ochratoxin A Ochratoxin B Zearalenone

CH3

OOHO OH

OO

NH

ClCH3

OOHO OH

OO

NH

H

O

OOH CH3H

HOO

Nivalenol HT-2-Toxin T-2-Toxin

H3C

O HO

H

OH

OHHO

O

H3COH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3

OH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3

O ndash CO ndash CH3

Patulin Fumonisins B1 R = OH B2 R = H

OHH

OO

O COOHCOOH

COOHCOOH

OH OHO

O

OR

O

NH2

CH3

CH3 CH3

H3C


Typical samples preparation T-2 toxin and HT-2 toxin from oat products in accordance with sect 64 LFGB (German Regulation for Food Analysis)Sample pretreatment25 plusmn 01 g of a ground homogenized sample (particle size lt 1 mm) are weighed into an Erlenmeyer flask or centrifuge tube 100 mL acetonitrile ndash water (8416 vv) is added The sample is mixed in a laboratory mixer (1 h RT) After ex-traction 10 mL of solution is filtered through a folded filter or centrifuged (2500 g RT)

Solid phase extractionMN AAp MNo 305560

c Column type CHROMABONDreg CarbonAloxCelitereg 6 mL 500 mg REF 730584 (on request) No conditioning or equilibration of the SPE column is necessary

Sample aspiration 5 mL of prepared sample is passed through the column by slight vacuum (1ndash2 dropss) into a silanized glass vialWashing 2 x 5 mL acetonitrile ndash water (8416 vv) these eluates are passed into the same silanized vial ie all eluates are com-bined

CHROMABONDreg vacuum manifold for 12 columns REF 730150

Transfer into a sample vial eg 15 mL screw neck vials N 9 amber flat bottom label and scale wide opening REF 702284 (silanized version REF 702079) and ready assembled screw closures N 9 blue center hole with septum silicone PTFE REF 7022871

116

x 3

2 m

m

It is recommended to filter the prepared sample through dis-posable filters CHROMAFILreg Xtra PVDF-4525 REF 729219

For other applications see wwwmn-netcomaAAs


Analysis Subsequent analysis HPLCMycotoxin exposure in Germany

MN AAp MNo 127240Column EC 1502 NUCLEODURreg C18 Pyramid 3 μm

REF 76026120

PeaksDON DeoxynivalenolDON-GlcA Deoxynivalenol-3-glucuronide

Deoxynivalenol-15-glucuronideT-2 T-2 toxinHT-2 HT-2 toxinHT-2-GlcA HT-2-toxin-4-glucuronideFB1 Fumonisin B1FB2 Fumonisin B2AFB1 Aflatoxin B1AFB2 Aflatoxin B2AFG2 Aflatoxin G2AFM1 Aflatoxin M1ZAN ZearalanoneZEN Zearalenoneα-ZEL α-Zearalenolβ-ZEL β-ZearalenolZEN-14-GlcA Zearalenone-14-O-glucuronideZAN-14-GlcA Zearalanone-14-O-glucuronideα-ZEL-14-GlcA α-Zearalenol-14-O-glucuronideβ-ZEL-14-GlcA β-Zearalenol-14-O-glucuronideOTA Ochratoxin AOTα Ochratoxin alphaEnB Enniatin BDH-CIT Dihydrocitrinone

Patulin and hydroxymethylfurfural from apple juiceMN AAp MNo 121800

Column EC 2504 NUCLEODURreg C18 Gravity 5 μm REF 76010140

0 2 4 6 8 min

1

2

standard

juice

Peaks1 Hydroxymethylfurfural2 Patulin

Detailed conditions are available online at wwwmn-netcomaAAs


Analysis Subsequent analysis HPLCFast separation of aflatoxins

MN AAp MNo 125600Column EC 502 NUCLEOSHELLreg PFP 27 μm

REF 76353220

10 2 3 min

1

2

3

4

Peaks1 Aflatoxin G22 Aflatoxin G13 Aflatoxin B24 Aflatoxin B1

MN suggests using guard columns to protect your main column and significantly increase column life

The Column Protection System (REF 718966) is suitable for all analytical HPLC columns with 116rdquo fittings



Product information Ordering informationStep Product Pack of REFSPE CHROMABONDreg CarbonAloxCelitereg 6 mL 500 mg (on request) 30 730584

CHROMABONDreg vacuum manifold for 12 columns 1 730150Filtration Disposable filters CHROMAFILreg Xtra PVDF-4525 100 729219Vials and caps 15 mL screw neck vials N 9 amber label and scale 100 702284

Vials N 9 as before silanized version 100 702079Ready assembled screw closures N 9 blue center hole with septum Silicone white PTFE red

100 7022871

HPLC 1Column EC 1502 NUCLEODURreg C18 Pyramid 3 μm 1 76026120Guard column EC 42 NUCLEODURreg C18 Pyramid 3 μm 3 76191620Guard column holder Column Protection System 1 718966HPLC 2Column EC 2504 NUCLEODURreg C18 Gravity 5 μm 1 76010140Guard column EC 43 NUCLEODURreg C18 Gravity 5 μm 3 76190330Guard column holder Column Protection System 1 718966HPLC 3Column EC 502 NUCLEOSHELLreg PFP 27 μm 1 76353220Guard column EC 42 NUCLEOSHELLreg PFP 27 μm 3 76353820Guard column holder Column Protection System 1 718966

Visit us at wwwmn-netcomchroma to get more helpful information

Selection tools for

Vials and caps

Syringe filters

Troubleshooting guidesGC and HPLC

Detailed product information

hellipand much more

wwwmn-netcom

wwwmn-netcomMACHEREY-NAGEL GmbH amp Co KG middot Neumann-Neander-Str 6ndash8 middot 52355 Duumlren middot GermanyFranceMACHEREY-NAGEL EURLTel +33 388 68 22 68Fax +33 388 51 76 88E-mail sales-frmn-netcom

SwitzerlandMACHEREY-NAGEL AGTel +41 62 388 55 00Fax +41 62 388 55 05E-mail sales-chmn-netcom

Germanyand internationalTel +49 24 21 969-0Fax +49 24 21 969-199E-mail infomn-netcom

MACHEREY-NAGEL

EN ISO 9001 2008CERTIFIED

USAMACHEREY-NAGEL IncTel +1 484 821 0984Fax +1 484 821 1272E-mail sales-usmn-netcom

Food safety made easy with products from

MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com


Focus on mycotoxins The global production and trading of food and feed products makes monitoring and control difficult

Only analysis of food and raw materials for the production of food can ensure safe and edible food Food safety is an essential part of our quality of life

MN shows examples for food analysis that will help you with your work and make our food safer

Vol 6 Mycotoxins in food infested with moldMycotoxins are secondary metabolites of fungi molds In contrast to environmental contaminants (pesticides PCBs PAHs etc) mycotoxins are inevitable More than 300 mycotoxins are chemically characterized [Food and Agriculture Organization of the United Nations (FAO) ndash httpwwwfaoorgdocrep005y1390ey1390e0bhtm] The follow-ing mycotoxins can be considered as the most common mycotoxins in feed and food

Aflatoxins (aflatoxin B1 B2 G1 and G2 aflatoxin M1) ochratoxin A (OTA) trichothecenes (Deoxynivalenol ndash

DON nivalenol T-2 toxin HT-2 toxin) patulin ergot al-kaloids zearalenone ndash ZEA fumonisins B1 and B2)

The presence of mycotoxins in food and feed may affect the health of humans and animals Many adverse health effects are suspected such as causing of cancer and mutations and excess estrogens gastrointestinal and kidney disorders [Food and Agriculture Organization of the United Nations (FAO) ndash httpwwwfaoorgwairdocsx5008ex5008e01htm]

Toxicological properties of selected mycotoxinsMycotoxin Possible effects

Aflatoxins Liver diseases (hepatotoxic hepatocarcinogenic) carcinogenic and teratogenic effects hemorrhages (intestinal tract kidneys) reduced growth rate diminution of performance immune suppression

Ochratoxins Nephrotoxic carcinogenic mild liver damage enteritis teratogenic effects poor feed conversion reduced growth rate immune suppression

Fumonisins Pulmonary edema equine leukoencephalomalacia nephro- and hepatotoxic immune suppression

Trichothecenes Digestive disorders (vomiting diarrhea feed refusal) reduced weight gain hemorrhages (stomach heart intestine lung bladder kidney) edema oral lesions dermatitis blood disorders infertility degeneration of bone marrow slow growth immune suppression

Zearalenone Estrogenic effects edema of vulva prolapse of vagina enlargement of uterus atrophy of testicles atrophy of ovaries enlargement of mammary glands infertility abortion

Ergot alkaloids Nervous or gangrenous syndromes Digestive disorders (vomiting diarrhea feed refusal) reduced weight gain convulsion abortion

Citrinin Nephrotoxic teratogenic hepatotoxic

Patulin Mutagenic genotoxic neurotoxic immune suppression

Richard J L (2007) Some major mycotoxins and their mycotoxicoses ndash an overview International Journal of Food Microbiology 119 (1ndash2) 3ndash10




OCH3

O

O

OO

O

OCH3

O

O

OO

O

OCH3

O

O

OO

O

OH


OCH3

O O

O O

OO OCH3

O O

O O

OO OCH3

O

O

OO

O

OH


CH3

OOHO OH

OO

NH

ClCH3

OOHO OH

OO

NH

H

O

OOH CH3H

HOO


H3C

O HO

H

OH

OHHO

O

H3COH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3

OH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3



OHH

OO

O COOHCOOH

COOHCOOH

OH OHO

O

OR

O

NH2

CH3

CH3 CH3

H3C








116

x 3

2 m

m






REF 76026120





0 2 4 6 8 min

1

2

standard

juice






REF 76353220

10 2 3 min

1

2

3

4









100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com




OCH3

O

O

OO

O

OCH3

O

O

OO

O

OCH3

O

O

OO

O

OH


OCH3

O O

O O

OO OCH3

O O

O O

OO OCH3

O

O

OO

O

OH


CH3

OOHO OH

OO

NH

ClCH3

OOHO OH

OO

NH

H

O

OOH CH3H

HOO


H3C

O HO

H

OH

OHHO

O

H3COH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3

OH

H3C

H3C

H3C

O

O

HO

H

OO

OHHO

O

H3C

CH3



OHH

OO

O COOHCOOH

COOHCOOH

OH OHO

O

OR

O

NH2

CH3

CH3 CH3

H3C








116

x 3

2 m

m






REF 76026120





0 2 4 6 8 min

1

2

standard

juice






REF 76353220

10 2 3 min

1

2

3

4









100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com








116

x 3

2 m

m






REF 76026120





0 2 4 6 8 min

1

2

standard

juice






REF 76353220

10 2 3 min

1

2

3

4









100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com




REF 76026120





0 2 4 6 8 min

1

2

standard

juice






REF 76353220

10 2 3 min

1

2

3

4









100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com




REF 76353220

10 2 3 min

1

2

3

4









100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com





100 7022871



Selection tools for

Vials and caps

Syringe filters



hellipand much more

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com

wwwmn-netcom




MACHEREY-NAGEL




MACHEREY-NAGEL

Vol 8

Additives

Vol 6

Mycotoxins

Vol 2

Vitamins

Vol 4

Acrylamide

Vol 1

Melamine

Vol 5

Sugars Sweeteners

Vol 3

AntibioticsVol 7

Pesticides

local distributor

KATE

N20

0122

Foc

us F

lyer

Foo

d Vo

l 6

en1

10

031

5 PD

Pr

inte

d in

Ger

man

yIm

age

cred

its copy

Tija

na o

lga

dem

schi

shin

a P

ierre

bril

lot

Pete

r Her

mes

Fur

ian

ar

enaf

otou

k fo

vito

nic

eday

rr1

45 a

irbor

ne77

ve

nusa

ngel

ndash fo

tolia

com

chromatograph - ftp.mn-net.comftp.mn-net.com/english/flyer_catalogs/...18 pyramid, 3 μm 1 760261.20...

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