CHROMATOGRAPHYAP BIO LAB #4A

Image by Riedellhttp://www.valemount.com/joel/lightoptics/images/colour6.jpg

PLANT PIGMENTS

.

http://members.aol.com/diaspore/vhouse/biology/projects/photosynthesis/woida/meaning_of_light.html#question_1

CHROMATOGRAPHY

USE PENCIL not pen, to mark starting line !!!!!

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ADD PLANT PIGMENTS

Image from Biology Place: http://www.phschool.com/science/biology_place/labbench/lab4/deposit.html

PLACE IN JARImage by Riedell

IMPORTANT:

Make sure tip of paper is touching solvent

Make sure pigment lineis ABOVE solvent

Put lid on jar !!!!!!!!

Important

http://www.phschool.com/science/biology_place/labbench/lab4/pigsep.html

Don’t let solvent front run off end of paper !

Marker Chromatography

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Rf =Distance pigment migratedDistance solvent front migrated

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PLANT PIGMENTS

Carotene is orange yellow

Xanthophyll is lemon yellow

Chlorophyll a is blue green

Chlorophyll b is yellow green.

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Factors that affect movement of molecules during chromatography

1. ___________of molecule in solvent used

(better it dissolves… farther it will move)

Solubility

SOLVENT used: Chlorophyll: 9:1 Petroleum ether : acetone

Markers: water

Factors that affect movement of molecules during chromatography

2. _________of molecule

(bigger molecules

move slower)

Size

http://elvis.phys.lsu.edu/svoboda/post/figs/SumoWrestler.BMP

Factors that affect movement of molecules during chromatography

3. _______________________ used

(EX: Attraction of molecules to paper)Attraction to media

http://courses.cm.utexas.edu/jrobertus/ch339k/overheads-1/ch5_mol-seive.jpghttp://www.cofc.edu/~kinard/Applets/GasChromatographFID.gif

Lots of different kinds of chromatography

Factors that affect movement of molecules during chromatography

4. __________allowed to runTime

http://www1.istockphoto.com/file_thumbview_approve/581259/2/istockphoto_581259_big_time.jpg

Longer time = More separation

CHLOROPHYLL FLUORESCENCE

Image from Biology AP Edition by Campbell & Reece published by Pearson Benjamin Cummings