chromatography product specialist jasco · powerpoint presentation author: tom dephillipo created...
TRANSCRIPT
-
About the PresenterTom DePhillipoChromatography Product Specialist JASCO
-
Alternate Detection TechniquesJASCO WEBINAR SERIES
-
Agenda (I) JASCO Overview
Products HPLC vs SFC Detection
Circular Dichroism Detection summary Operating principal Applications
Refractive Index Detection summary Operating principal Applications
-
Agenda (II) Optical Rotation
Detection Summary Operating principal Applications
Fluorescence Detection Summary Operating principal Applications
Evaporative light scattering detection (ELSD) Detection summary Operating principal Applications
-
Founding Members1958 Established at the Optical Research Institute at Tsukuba University, TokyoFounding members include: World famous physicist Yoshio
Fujioka Nobel Prize winner Shinichiro
Tomonaga (1965 - Physics for QED with Richard Feynman)
1972JASCO in the USAHPLC Products
Dr. Tomonaga
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JASCO Products
-
SFC1 MS
1. Cooled CO2 Pump2. High Pressure Flow Cell3. Back Pressure Regulator
HPLC vs SFCHPLC
MS
-
Circular Dichroism(HPLC and SFC)
-
Detection Summary Circular Dichroism detection offers high sensitivity for chiral compounds
Chiral compounds absorb to a different extent for left and right circularly polarized light
The detection is based on the difference between right-hand and left-hand circular polarizations
This technique is relatively insensitive to dynamic temperature and solvent conditions (gradients)
This detector is unique to JASCO
-
Operating Principal The light emitted by light source is collimated by
the lens and introduced to the polarizer where the light is linearly polarized
The light is then dispersed by the diffraction grating and directed to pass through the photo-elastic modulator (PEM)
The PEM modulates the light to left and right circularly polarized light (LCP and RCP) which are alternately changed at an interval of 50 kHZ
The modulated light is directed to the flow cell by the lens
Flow Cell
-
Diagram of Time Variation Light Intensities
Example of signal when no active CD compound is in flow cell
Example of signal when active CD compound is in flow cell
-
220 250 300 350 400
Chiral Separation of Flavanone (1µg) and CD Spectra
310nm250 nm
340 nm
CD
0370 nm
Wavelength
250nm S/N=188.0
310nm S/N=210.8
340nm S/N=44.4
370nm S/N=28.3
Note: detector distinguishes enantiomers regardless of which indicated wavelength is chosen, something UV would miss
12.0
[min]
1.0
-1.0
0.0
-4.0
0.0
4.0
-10.0
-10.0
-5.0
0.0
5.0
-5.0
0.0
5.0
mV
4.0
8.0
-
g-Factor Calculation• g-factor = ΔAU (CD) / AU (UV)
g-factor is proportional to optical purity (EE%) g-factor is independent of Concentration
• g-factor trace indicates optical purity during elution, offering cut-off timing for preparative LC.
• Simultaneous CD and UV detection allows optical purity measurements/collection without complete chiral separation.
-
-40
-20
0
20
40
60
80
5.4 5.8 6.2 6.6
g factorCD(230nm)UV(230nm)
Incomplete Separation, Ibuprofen (1 µg)
ConditionsColumn : CHRALCEL OD (4.6 x 250 mm)Eluent : n-Hexane / IPA / TFA (100 / 4 / 0.1) Flow rate : 1.0 mL/minTemp. : 50 ℃Sample : Ibuprofen (1μg)
UV
(mAU
) and
CD
(mde
g)Ｘ
5
Time (min)
0.0E+00
10.0.0E-06
5.0E-06
-5.0E-06
-10.0E-06
Optically pure areas When pure
enantiomer is eluting the ratio of CD/UV is constant and therefore produces a flat line
Graph1
5.45.45.4
5.40335.40335.4033
5.40675.40675.4067
5.415.415.41
5.41335.41335.4133
5.41675.41675.4167
5.425.425.42
5.42335.42335.4233
5.42675.42675.4267
5.435.435.43
5.43335.43335.4333
5.43675.43675.4367
5.445.445.44
5.44335.44335.4433
5.44675.44675.4467
5.455.455.45
5.45335.45335.4533
5.45675.45675.4567
5.465.465.46
5.46335.46335.4633
5.46675.46675.4667
5.475.475.47
5.47335.47335.4733
5.47675.47675.4767
5.485.485.48
5.48335.48335.4833
5.48675.48675.4867
5.495.495.49
5.49335.49335.4933
5.49675.49675.4967
5.55.55.5
5.50335.50335.5033
5.50675.50675.5067
5.515.515.51
5.51335.51335.5133
5.51675.51675.5167
5.525.525.52
5.52335.52335.5233
5.52675.52675.5267
5.535.535.53
5.53335.53335.5333
5.53675.53675.5367
5.545.545.54
5.54335.54335.5433
5.54675.54675.5467
5.555.555.55
5.55335.55335.5533
5.55675.55675.5567
5.565.565.56
5.56335.56335.5633
5.56675.56675.5667
5.575.575.57
5.57335.57335.5733
5.57675.57675.5767
5.585.585.58
5.58335.58335.5833
5.58675.58675.5867
5.595.595.59
5.59335.59335.5933
5.59675.59675.5967
5.65.65.6
5.60335.60335.6033
5.60675.60675.6067
5.615.615.61
5.61335.61335.6133
5.61675.61675.6167
5.625.625.62
5.62335.62335.6233
5.62675.62675.6267
5.635.635.63
5.63335.63335.6333
5.63675.63675.6367
5.645.645.64
5.64335.64335.6433
5.64675.64675.6467
5.655.655.65
5.65335.65335.6533
5.65675.65675.6567
5.665.665.66
5.66335.66335.6633
5.66675.66675.6667
5.675.675.67
5.67335.67335.6733
5.67675.67675.6767
5.685.685.68
5.68335.68335.6833
5.68675.68675.6867
5.695.695.69
5.69335.69335.6933
5.69675.69675.6967
5.75.75.7
5.70335.70335.7033
5.70675.70675.7067
5.715.715.71
5.71335.71335.7133
5.71675.71675.7167
5.725.725.72
5.72335.72335.7233
5.72675.72675.7267
5.735.735.73
5.73335.73335.7333
5.73675.73675.7367
5.745.745.74
5.74335.74335.7433
5.74675.74675.7467
5.755.755.75
5.75335.75335.7533
5.75675.75675.7567
5.765.765.76
5.76335.76335.7633
5.76675.76675.7667
5.775.775.77
5.77335.77335.7733
5.77675.77675.7767
5.785.785.78
5.78335.78335.7833
5.78675.78675.7867
5.795.795.79
5.79335.79335.7933
5.79675.79675.7967
5.85.85.8
5.80335.80335.8033
5.80675.80675.8067
5.815.815.81
5.81335.81335.8133
5.81675.81675.8167
5.825.825.82
5.82335.82335.8233
5.82675.82675.8267
5.835.835.83
5.83335.83335.8333
5.83675.83675.8367
5.845.845.84
5.84335.84335.8433
5.84675.84675.8467
5.855.855.85
5.85335.85335.8533
5.85675.85675.8567
5.865.865.86
5.86335.86335.8633
5.86675.86675.8667
5.875.875.87
5.87335.87335.8733
5.87675.87675.8767
5.885.885.88
5.88335.88335.8833
5.88675.88675.8867
5.895.895.89
5.89335.89335.8933
5.89675.89675.8967
5.95.95.9
5.90335.90335.9033
5.90675.90675.9067
5.915.915.91
5.91335.91335.9133
5.91675.91675.9167
5.925.925.92
5.92335.92335.9233
5.92675.92675.9267
5.935.935.93
5.93335.93335.9333
5.93675.93675.9367
5.945.945.94
5.94335.94335.9433
5.94675.94675.9467
5.955.955.95
5.95335.95335.9533
5.95675.95675.9567
5.965.965.96
5.96335.96335.9633
5.96675.96675.9667
5.975.975.97
5.97335.97335.9733
5.97675.97675.9767
5.985.985.98
5.98335.98335.9833
5.98675.98675.9867
5.995.995.99
5.99335.99335.9933
5.99675.99675.9967
666
6.00336.00336.0033
6.00676.00676.0067
6.016.016.01
6.01336.01336.0133
6.01676.01676.0167
6.026.026.02
6.02336.02336.0233
6.02676.02676.0267
6.036.036.03
6.03336.03336.0333
6.03676.03676.0367
6.046.046.04
6.04336.04336.0433
6.04676.04676.0467
6.056.056.05
6.05336.05336.0533
6.05676.05676.0567
6.066.066.06
6.06336.06336.0633
6.06676.06676.0667
6.076.076.07
6.07336.07336.0733
6.07676.07676.0767
6.086.086.08
6.08336.08336.0833
6.08676.08676.0867
6.096.096.09
6.09336.09336.0933
6.09676.09676.0967
6.16.16.1
6.10336.10336.1033
6.10676.10676.1067
6.116.116.11
6.11336.11336.1133
6.11676.11676.1167
6.126.126.12
6.12336.12336.1233
6.12676.12676.1267
6.136.136.13
6.13336.13336.1333
6.13676.13676.1367
6.146.146.14
6.14336.14336.1433
6.14676.14676.1467
6.156.156.15
6.15336.15336.1533
6.15676.15676.1567
6.166.166.16
6.16336.16336.1633
6.16676.16676.1667
6.176.176.17
6.17336.17336.1733
6.17676.17676.1767
6.186.186.18
6.18336.18336.1833
6.18676.18676.1867
6.196.196.19
6.19336.19336.1933
6.19676.19676.1967
6.26.26.2
6.20336.20336.2033
6.20676.20676.2067
6.216.216.21
6.21336.21336.2133
6.21676.21676.2167
6.226.226.22
6.22336.22336.2233
6.22676.22676.2267
6.236.236.23
6.23336.23336.2333
6.23676.23676.2367
6.246.246.24
6.24336.24336.2433
6.24676.24676.2467
6.256.256.25
6.25336.25336.2533
6.25676.25676.2567
6.266.266.26
6.26336.26336.2633
6.26676.26676.2667
6.276.276.27
6.27336.27336.2733
6.27676.27676.2767
6.286.286.28
6.28336.28336.2833
6.28676.28676.2867
6.296.296.29
6.29336.29336.2933
6.29676.29676.2967
6.36.36.3
6.30336.30336.3033
6.30676.30676.3067
6.316.316.31
6.31336.31336.3133
6.31676.31676.3167
6.326.326.32
6.32336.32336.3233
6.32676.32676.3267
6.336.336.33
6.33336.33336.3333
6.33676.33676.3367
6.346.346.34
6.34336.34336.3433
6.34676.34676.3467
6.356.356.35
6.35336.35336.3533
6.35676.35676.3567
6.366.366.36
6.36336.36336.3633
6.36676.36676.3667
6.376.376.37
6.37336.37336.3733
6.37676.37676.3767
6.386.386.38
6.38336.38336.3833
6.38676.38676.3867
6.396.396.39
6.39336.39336.3933
6.39676.39676.3967
6.46.46.4
6.40336.40336.4033
6.40676.40676.4067
6.416.416.41
6.41336.41336.4133
6.41676.41676.4167
6.426.426.42
6.42336.42336.4233
6.42676.42676.4267
6.436.436.43
6.43336.43336.4333
6.43676.43676.4367
6.446.446.44
6.44336.44336.4433
6.44676.44676.4467
6.456.456.45
6.45336.45336.4533
6.45676.45676.4567
6.466.466.46
6.46336.46336.4633
6.46676.46676.4667
6.476.476.47
6.47336.47336.4733
6.47676.47676.4767
6.486.486.48
6.48336.48336.4833
6.48676.48676.4867
6.496.496.49
6.49336.49336.4933
6.49676.49676.4967
6.56.56.5
6.50336.50336.5033
6.50676.50676.5067
6.516.516.51
6.51336.51336.5133
6.51676.51676.5167
6.526.526.52
6.52336.52336.5233
6.52676.52676.5267
6.536.536.53
6.53336.53336.5333
6.53676.53676.5367
6.546.546.54
6.54336.54336.5433
6.54676.54676.5467
6.556.556.55
6.55336.55336.5533
6.55676.55676.5567
6.566.566.56
6.56336.56336.5633
6.56676.56676.5667
6.576.576.57
6.57336.57336.5733
6.57676.57676.5767
6.586.586.58
6.58336.58336.5833
6.58676.58676.5867
6.596.596.59
6.59336.59336.5933
6.59676.59676.5967
6.66.66.6
6.60336.60336.6033
6.60676.60676.6067
6.616.616.61
6.61336.61336.6133
6.61676.61676.6167
6.626.626.62
6.62336.62336.6233
6.62676.62676.6267
6.636.636.63
6.63336.63336.6333
6.63676.63676.6367
6.646.646.64
6.64336.64336.6433
6.64676.64676.6467
6.656.656.65
6.65336.65336.6533
6.65676.65676.6567
6.666.666.66
6.66336.66336.6633
6.66676.66676.6667
6.676.676.67
6.67336.67336.6733
6.67676.67676.6767
6.686.686.68
6.68336.68336.6833
6.68676.68676.6867
6.696.696.69
6.69336.69336.6933
6.69676.69676.6967
6.76.76.7
6.70336.70336.7033
6.70676.70676.7067
6.716.716.71
6.71336.71336.7133
6.71676.71676.7167
6.726.726.72
6.72336.72336.7233
6.72676.72676.7267
6.736.736.73
6.73336.73336.7333
6.73676.73676.7367
6.746.746.74
6.74336.74336.7433
6.74676.74676.7467
6.756.756.75
6.75336.75336.7533
6.75676.75676.7567
6.766.766.76
6.76336.76336.7633
6.76676.76676.7667
6.776.776.77
6.77336.77336.7733
6.77676.77676.7767
6.786.786.78
6.78336.78336.7833
6.78676.78676.7867
6.796.796.79
6.79336.79336.7933
6.79676.79676.7967
6.86.86.8
6.80336.80336.8033
6.80676.80676.8067
6.816.816.81
6.81336.81336.8133
6.81676.81676.8167
6.826.826.82
6.82336.82336.8233
6.82676.82676.8267
6.836.836.83
6.83336.83336.8333
6.83676.83676.8367
6.846.846.84
6.84336.84336.8433
6.84676.84676.8467
6.856.856.85
6.85336.85336.8533
6.85676.85676.8567
6.866.866.86
6.86336.86336.8633
6.86676.86676.8667
6.876.876.87
6.87336.87336.8733
6.87676.87676.8767
6.886.886.88
6.88336.88336.8833
6.88676.88676.8867
6.896.896.89
6.89336.89336.8933
6.89676.89676.8967
6.96.96.9
&A
- &P -
Time(min)
g-factor, Ibuprofen
0
0
0
0.008
-0.768
-0.0000003146
0.027
-0.192
-0.0000042469
0.006
-0.512
-0.0000003539
-0.011
-0.896
0.0000003708
-0.033
-1.216
0.0000008196
-0.018
-1.6
0.0000003398
0.016
-1.024
-0.0000004719
0.013
-0.32
-0.0000012269
-0.03
-0.832
0.0000010889
-0.046
-0.512
0.0000027133
-0.047
-0.448
0.0000031683
-0.04
-0.64
0.0000018875
-0.023
-0.704
0.0000009866
-0.04
-0.32
0.000003775
-0.094
0.064
-0.0000443562
-0.074
-0.512
0.0000043648
-0.035
-0.32
0.0000033031
-0.019
-0.512
0.0000011207
-0.037
-0.384
0.0000029099
-0.078
-0.32
0.0000073613
-0.078
-0.128
0.0000184031
-0.051
-0.448
0.0000034379
-0.072
0.064
-0.000033975
-0.107
0.32
-0.0000100981
-0.115
0.128
-0.0000271328
-0.113
0.192
-0.000017774
-0.111
0.128
-0.0000261891
-0.135
0.448
-0.0000091004
-0.171
0.64
-0.0000080691
-0.188
0.704
-0.0000080648
-0.196
0.704
-0.000008408
-0.182
1.28
-0.0000042941
-0.216
2.048
-0.0000031852
-0.28
2.048
-0.0000041289
-0.342
1.856
-0.0000055649
-0.373
2.88
-0.0000039113
-0.422
3.584
-0.0000035559
-0.48
3.392
-0.0000042736
-0.524
3.968
-0.0000039881
-0.599
4.352
-0.0000041567
-0.652
5.248
-0.000003752
-0.697
6.208
-0.0000033907
-0.75
6.912
-0.0000032769
-0.805
6.912
-0.0000035172
-0.866
8.192
-0.0000031925
-0.938
9.28
-0.0000030525
-1.02
10.496
-0.0000029348
-1.096
11.136
-0.0000029723
-1.187
11.584
-0.0000030946
-1.278
12.352
-0.0000031246
-1.379
14.4
-0.0000028921
-1.508
15.296
-0.0000029774
-1.622
16.192
-0.0000030252
-1.72
17.216
-0.0000030172
-1.868
18.816
-0.0000029982
-1.975
20.608
-0.0000028943
-2.111
21.632
-0.0000029471
-2.293
23.232
-0.0000029807
-2.446
24.832
-0.0000029748
-2.574
25.984
-0.0000029916
-2.683
27.072
-0.000002993
-2.824
28.736
-0.0000029679
-2.958
30.08
-0.0000029698
-3.114
32.256
-0.0000029155
-3.25
33.728
-0.00000291
-3.37
34.56
-0.0000029448
-3.5
35.84
-0.0000029492
-3.624
37.632
-0.0000029083
-3.761
39.488
-0.0000028764
-3.913
40.32
-0.0000029309
-4.054
41.472
-0.0000029521
-4.159
43.008
-0.0000029204
-4.242
44.672
-0.0000028678
-4.363
45.12
-0.0000029203
-4.483
46.72
-0.0000028978
-4.602
47.872
-0.0000029032
-4.74
48.576
-0.0000029469
-4.823
49.792
-0.0000029253
-4.848
51.008
-0.0000028703
-4.967
51.584
-0.0000029079
-5.05
52.992
-0.000002878
-5.129
53.376
-0.000002902
-5.165
54.272
-0.0000028741
-5.215
54.272
-0.0000029019
-5.232
55.168
-0.0000028641
-5.287
55.68
-0.0000028676
-5.367
54.976
-0.0000029483
-5.38
56.064
-0.000002898
-5.428
56.32
-0.0000029106
-5.438
56.128
-0.0000029259
-5.467
56.96
-0.0000028986
-5.477
56.576
-0.0000029236
-5.461
56.896
-0.0000028987
-5.429
56.384
-0.0000029078
-5.454
56.192
-0.0000029312
-5.438
56.512
-0.0000029061
-5.409
56.128
-0.0000029103
-5.391
55.424
-0.0000029375
-5.378
55.488
-0.000002927
-5.332
54.72
-0.0000029427
-5.316
54.848
-0.0000029271
-5.271
54.016
-0.000002947
-5.177
53.888
-0.0000029013
-5.088
53.696
-0.0000028616
-5.046
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Graph2
5.45.45.4
5.40335.40335.4033
5.40675.40675.4067
5.415.415.41
5.41335.41335.4133
5.41675.41675.4167
5.425.425.42
5.42335.42335.4233
5.42675.42675.4267
5.435.435.43
5.43335.43335.4333
5.43675.43675.4367
5.445.445.44
5.44335.44335.4433
5.44675.44675.4467
5.455.455.45
5.45335.45335.4533
5.45675.45675.4567
5.465.465.46
5.46335.46335.4633
5.46675.46675.4667
5.475.475.47
5.47335.47335.4733
5.47675.47675.4767
5.485.485.48
5.48335.48335.4833
5.48675.48675.4867
5.495.495.49
5.49335.49335.4933
5.49675.49675.4967
5.55.55.5
5.50335.50335.5033
5.50675.50675.5067
5.515.515.51
5.51335.51335.5133
5.51675.51675.5167
5.525.525.52
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5.535.535.53
5.53335.53335.5333
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5.545.545.54
5.54335.54335.5433
5.54675.54675.5467
5.555.555.55
5.55335.55335.5533
5.55675.55675.5567
5.565.565.56
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5.56675.56675.5667
5.575.575.57
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5.57675.57675.5767
5.585.585.58
5.58335.58335.5833
5.58675.58675.5867
5.595.595.59
5.59335.59335.5933
5.59675.59675.5967
5.65.65.6
5.60335.60335.6033
5.60675.60675.6067
5.615.615.61
5.61335.61335.6133
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5.625.625.62
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5.635.635.63
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5.645.645.64
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5.655.655.65
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5.665.665.66
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5.66675.66675.6667
5.675.675.67
5.67335.67335.6733
5.67675.67675.6767
5.685.685.68
5.68335.68335.6833
5.68675.68675.6867
5.695.695.69
5.69335.69335.6933
5.69675.69675.6967
5.75.75.7
5.70335.70335.7033
5.70675.70675.7067
5.715.715.71
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5.71675.71675.7167
5.725.725.72
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5.745.745.74
5.74335.74335.7433
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5.755.755.75
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5.765.765.76
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5.76675.76675.7667
5.775.775.77
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5.785.785.78
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5.78675.78675.7867
5.795.795.79
5.79335.79335.7933
5.79675.79675.7967
5.85.85.8
5.80335.80335.8033
5.80675.80675.8067
5.815.815.81
5.81335.81335.8133
5.81675.81675.8167
5.825.825.82
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5.835.835.83
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5.845.845.84
5.84335.84335.8433
5.84675.84675.8467
5.855.855.85
5.85335.85335.8533
5.85675.85675.8567
5.865.865.86
5.86335.86335.8633
5.86675.86675.8667
5.875.875.87
5.87335.87335.8733
5.87675.87675.8767
5.885.885.88
5.88335.88335.8833
5.88675.88675.8867
5.895.895.89
5.89335.89335.8933
5.89675.89675.8967
5.95.95.9
5.90335.90335.9033
5.90675.90675.9067
5.915.915.91
5.91335.91335.9133
5.91675.91675.9167
5.925.925.92
5.92335.92335.9233
5.92675.92675.9267
5.935.935.93
5.93335.93335.9333
5.93675.93675.9367
5.945.945.94
5.94335.94335.9433
5.94675.94675.9467
5.955.955.95
5.95335.95335.9533
5.95675.95675.9567
5.965.965.96
5.96335.96335.9633
5.96675.96675.9667
5.975.975.97
5.97335.97335.9733
5.97675.97675.9767
5.985.985.98
5.98335.98335.9833
5.98675.98675.9867
5.995.995.99
5.99335.99335.9933
5.99675.99675.9967
666
6.00336.00336.0033
6.00676.00676.0067
6.016.016.01
6.01336.01336.0133
6.01676.01676.0167
6.026.026.02
6.02336.02336.0233
6.02676.02676.0267
6.036.036.03
6.03336.03336.0333
6.03676.03676.0367
6.046.046.04
6.04336.04336.0433
6.04676.04676.0467
6.056.056.05
6.05336.05336.0533
6.05676.05676.0567
6.066.066.06
6.06336.06336.0633
6.06676.06676.0667
6.076.076.07
6.07336.07336.0733
6.07676.07676.0767
6.086.086.08
6.08336.08336.0833
6.08676.08676.0867
6.096.096.09
6.09336.09336.0933
6.09676.09676.0967
6.16.16.1
6.10336.10336.1033
6.10676.10676.1067
6.116.116.11
6.11336.11336.1133
6.11676.11676.1167
6.126.126.12
6.12336.12336.1233
6.12676.12676.1267
6.136.136.13
6.13336.13336.1333
6.13676.13676.1367
6.146.146.14
6.14336.14336.1433
6.14676.14676.1467
6.156.156.15
6.15336.15336.1533
6.15676.15676.1567
6.166.166.16
6.16336.16336.1633
6.16676.16676.1667
6.176.176.17
6.17336.17336.1733
6.17676.17676.1767
6.186.186.18
6.18336.18336.1833
6.18676.18676.1867
6.196.196.19
6.19336.19336.1933
6.19676.19676.1967
6.26.26.2
6.20336.20336.2033
6.20676.20676.2067
6.216.216.21
6.21336.21336.2133
6.21676.21676.2167
6.226.226.22
6.22336.22336.2233
6.22676.22676.2267
6.236.236.23
6.23336.23336.2333
6.23676.23676.2367
6.246.246.24
6.24336.24336.2433
6.24676.24676.2467
6.256.256.25
6.25336.25336.2533
6.25676.25676.2567
6.266.266.26
6.26336.26336.2633
6.26676.26676.2667
6.276.276.27
6.27336.27336.2733
6.27676.27676.2767
6.286.286.28
6.28336.28336.2833
6.28676.28676.2867
6.296.296.29
6.29336.29336.2933
6.29676.29676.2967
6.36.36.3
6.30336.30336.3033
6.30676.30676.3067
6.316.316.31
6.31336.31336.3133
6.31676.31676.3167
6.326.326.32
6.32336.32336.3233
6.32676.32676.3267
6.336.336.33
6.33336.33336.3333
6.33676.33676.3367
6.346.346.34
6.34336.34336.3433
6.34676.34676.3467
6.356.356.35
6.35336.35336.3533
6.35676.35676.3567
6.366.366.36
6.36336.36336.3633
6.36676.36676.3667
6.376.376.37
6.37336.37336.3733
6.37676.37676.3767
6.386.386.38
6.38336.38336.3833
6.38676.38676.3867
6.396.396.39
6.39336.39336.3933
6.39676.39676.3967
6.46.46.4
6.40336.40336.4033
6.40676.40676.4067
6.416.416.41
6.41336.41336.4133
6.41676.41676.4167
6.426.426.42
6.42336.42336.4233
6.42676.42676.4267
6.436.436.43
6.43336.43336.4333
6.43676.43676.4367
6.446.446.44
6.44336.44336.4433
6.44676.44676.4467
6.456.456.45
6.45336.45336.4533
6.45676.45676.4567
6.466.466.46
6.46336.46336.4633
6.46676.46676.4667
6.476.476.47
6.47336.47336.4733
6.47676.47676.4767
6.486.486.48
6.48336.48336.4833
6.48676.48676.4867
6.496.496.49
6.49336.49336.4933
6.49676.49676.4967
6.56.56.5
6.50336.50336.5033
6.50676.50676.5067
6.516.516.51
6.51336.51336.5133
6.51676.51676.5167
6.526.526.52
6.52336.52336.5233
6.52676.52676.5267
6.536.536.53
6.53336.53336.5333
6.53676.53676.5367
6.546.546.54
6.54336.54336.5433
6.54676.54676.5467
6.556.556.55
6.55336.55336.5533
6.55676.55676.5567
6.566.566.56
6.56336.56336.5633
6.56676.56676.5667
6.576.576.57
6.57336.57336.5733
6.57676.57676.5767
6.586.586.58
6.58336.58336.5833
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6.596.596.59
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6.66.66.6
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6.616.616.61
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6.636.636.63
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6.646.646.64
6.64336.64336.6433
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6.656.656.65
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6.666.666.66
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6.686.686.68
6.68336.68336.6833
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6.696.696.69
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6.76.76.7
6.70336.70336.7033
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6.716.716.71
6.71336.71336.7133
6.71676.71676.7167
6.726.726.72
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6.72676.72676.7267
6.736.736.73
6.73336.73336.7333
6.73676.73676.7367
6.746.746.74
6.74336.74336.7433
6.74676.74676.7467
6.756.756.75
6.75336.75336.7533
6.75676.75676.7567
6.766.766.76
6.76336.76336.7633
6.76676.76676.7667
6.776.776.77
6.77336.77336.7733
6.77676.77676.7767
6.786.786.78
6.78336.78336.7833
6.78676.78676.7867
6.796.796.79
6.79336.79336.7933
6.79676.79676.7967
6.86.86.8
6.80336.80336.8033
6.80676.80676.8067
6.816.816.81
6.81336.81336.8133
6.81676.81676.8167
6.826.826.82
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6.82676.82676.8267
6.836.836.83
6.83336.83336.8333
6.83676.83676.8367
6.846.846.84
6.84336.84336.8433
6.84676.84676.8467
6.856.856.85
6.85336.85336.8533
6.85676.85676.8567
6.866.866.86
6.86336.86336.8633
6.86676.86676.8667
6.876.876.87
6.87336.87336.8733
6.87676.87676.8767
6.886.886.88
6.88336.88336.8833
6.88676.88676.8867
6.896.896.89
6.89336.89336.8933
6.89676.89676.8967
6.96.96.9
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0.0000027362
8.205
17.216
0.000002833
8.075
16.768
0.0000027772
7.71
15.936
0.0000027763
7.325
15.872
0.000002797
7.35
15.296
0.0000027681
7.01
15.104
0.0000026753
6.69
14.08
0.0000027197
6.34
13.824
0.0000026041
5.96
13.248
0.0000025577
5.61
13.056
0.0000025814
5.58
12.352
0.000002665
5.45
11.648
0.0000025875
4.99
11.072
0.0000026049
4.775
10.816
0.0000026051
4.665
10.304
0.0000025968
4.43
9.792
0.0000027264
4.42
9.344
0.0000028991
4.485
9.28
0.0000027694
4.255
7.936
0.0000031205
4.1
8.448
0.0000028992
4.055
8.768
0.0000026866
3.9
7.488
0.00000292
3.62
7.488
0.0000028554
3.54
6.976
0.0000028096
3.245
6.656
0.0000025862
2.85
6.272
0.0000024653
2.56
6.4
0.0000022084
2.34
6.72
0.0000020718
2.305
5.696
0.0000024654
2.325
5.312
0.0000027005
2.375
5.76
0.0000024328
2.32
5.248
0.000002578
2.24
5.184
0.000002505
2.15
4.608
0.0000027723
2.115
3.712
0.0000034902
2.145
3.584
0.0000034042
2.02
4.288
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1.84
3.968
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1.49
3.84
0.0000021234
1.35
3.392
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1.325
3.264
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1.43
3.328
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1.46
3.2
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2.944
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1.12
2.688
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1
2.816
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2.752
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0.93
2.624
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2.432
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0.71
1.92
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0.75
2.24
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0.77
2.24
0.0000017662
0.655
2.24
0.0000020628
0.765
1.792
0.0000024268
0.72
1.28
0.0000030436
0.645
2.048
0.0000016221
0.55
1.92
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0.46
1.856
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0.285
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0.0000008116
0.215
1.536
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0.275
1.28
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0.24
1.024
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0.28
1.6
0.0000009437
0.25
0.896
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0.235
1.472
0.0000007181
0.175
0.768
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1.152
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1.024
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1.088
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IBUP016-1
Time(min)CDUV(CD+260)/1000(UV+286)*64/1000ΔAUg-factor
5.4-260-28600000
5.4033-252-2980.008-0.7680.0000002416-0.00000031460.04
5.4067-233-2890.027-0.1920.0000008154-0.00000424690.135
5.41-254-2940.006-0.5120.0000001812-0.00000035390.03
5.4133-271-300-0.011-0.896-0.00000033220.0000003708-0.055
5.4167-293-305-0.033-1.216-0.00000099660.0000008196-0.165
5.42-278-311-0.018-1.6-0.00000054360.0000003398-0.09
5.4233-244-3020.016-1.0240.0000004832-0.00000047190.08
5.4267-247-2910.013-0.320.0000003926-0.00000122690.065
5.43-290-299-0.03-0.832-0.0000009060.0000010889-0.15
5.4333-306-294-0.046-0.512-0.00000138920.0000027133-0.23
5.4367-307-293-0.047-0.448-0.00000141940.0000031683-0.235
5.44-300-296-0.04-0.64-0.0000012080.0000018875-0.2
5.4433-283-297-0.023-0.704-0.00000069460.0000009866-0.115
5.4467-300-291-0.04-0.32-0.0000012080.000003775-0.2
5.45-354-285-0.0940.064-0.0000028388-0.0000443562-0.47
5.4533-334-294-0.074-0.512-0.00000223480.0000043648-0.37
5.4567-295-291-0.035-0.32-0.0000010570.0000033031-0.175
5.46-279-294-0.019-0.512-0.00000057380.0000011207-0.095
5.4633-297-292-0.037-0.384-0.00000111740.0000029099-0.185
5.4667-338-291-0.078-0.32-0.00000235560.0000073613-0.39
5.47-338-288-0.078-0.128-0.00000235560.0000184031-0.39
5.4733-311-293-0.051-0.448-0.00000154020.0000034379-0.255
5.4767-332-285-0.0720.064-0.0000021744-0.000033975-0.36
5.48-367-281-0.1070.32-0.0000032314-0.0000100981-0.535
5.4833-375-284-0.1150.128-0.000003473-0.0000271328-0.575
5.4867-373-283-0.1130.192-0.0000034126-0.000017774-0.565
5.49-371-284-0.1110.128-0.0000033522-0.0000261891-0.555
5.4933-395-279-0.1350.448-0.000004077-0.0000091004-0.675
5.4967-431-276-0.1710.64-0.0000051642-0.0000080691-0.855
5.5-448-275-0.1880.704-0.0000056776-0.0000080648-0.94
5.5033-456-275-0.1960.704-0.0000059192-0.000008408-0.98
5.5067-442-266-0.1821.28-0.0000054964-0.0000042941-0.91
5.51-476-254-0.2162.048-0.0000065232-0.0000031852-1.08
5.5133-540-254-0.282.048-0.000008456-0.0000041289-1.4
5.5167-602-257-0.3421.856-0.0000103284-0.0000055649-1.71
5.52-633-241-0.3732.88-0.0000112646-0.0000039113-1.865
5.5233-682-230-0.4223.584-0.0000127444-0.0000035559-2.11
5.5267-740-233-0.483.392-0.000014496-0.0000042736-2.4
5.53-784-224-0.5243.968-0.0000158248-0.0000039881-2.62
5.5333-859-218-0.5994.352-0.0000180898-0.0000041567-2.995
5.5367-912-204-0.6525.248-0.0000196904-0.000003752-3.26
5.54-957-189-0.6976.208-0.0000210494-0.0000033907-3.485
5.5433-1010-178-0.756.912-0.00002265-0.0000032769-3.75
5.5467-1065-178-0.8056.912-0.000024311-0.0000035172-4.025
5.55-1126-158-0.8668.192-0.0000261532-0.0000031925-4.33
5.5533-1198-141-0.9389.28-0.0000283276-0.0000030525-4.69
5.5567-1280-122-1.0210.496-0.000030804-0.0000029348-5.1
5.56-1356-112-1.09611.136-0.0000330992-0.0000029723-5.48
5.5633-1447-105-1.18711.584-0.0000358474-0.0000030946-5.935
5.5667-1538-93-1.27812.352-0.0000385956-0.0000031246-6.39
5.57-1639-61-1.37914.4-0.0000416458-0.0000028921-6.895
5.5733-1768-47-1.50815.296-0.0000455416-0.0000029774-7.54
5.5767-1882-33-1.62216.192-0.0000489844-0.0000030252-8.11
5.58-1980-17-1.7217.216-0.000051944-0.0000030172-8.6
5.5833-21288-1.86818.816-0.0000564136-0.0000029982-9.34
5.5867-223536-1.97520.608-0.000059645-0.0000028943-9.875
5.59-237152-2.11121.632-0.0000637522-0.0000029471-10.555
5.5933-255377-2.29323.232-0.0000692486-0.0000029807-11.465
5.5967-2706102-2.44624.832-0.0000738692-0.0000029748-12.23
5.6-2834120-2.57425.984-0.0000777348-0.0000029916-12.87
5.6033-2943137-2.68327.072-0.0000810266-0.000002993-13.415
5.6067-3084163-2.82428.736-0.0000852848-0.0000029679-14.12
5.61-3218184-2.95830.08-0.0000893316-0.0000029698-14.79
5.6133-3374218-3.11432.256-0.0000940428-0.0000029155-15.57
5.6167-3510241-3.2533.728-0.00009815-0.00000291-16.25
5.62-3630254-3.3734.56-0.000101774-0.0000029448-16.85
5.6233-3760274-3.535.84-0.0001057-0.0000029492-17.5
5.6267-3884302-3.62437.632-0.0001094448-0.0000029083-18.12
5.63-4021331-3.76139.488-0.0001135822-0.0000028764-18.805
5.6333-4173344-3.91340.32-0.0001181726-0.0000029309-19.565
5.6367-4314362-4.05441.472-0.0001224308-0.0000029521-20.27
5.64-4419386-4.15943.008-0.0001256018-0.0000029204-20.795
5.6433-4502412-4.24244.672-0.0001281084-0.0000028678-21.21
5.6467-4623419-4.36345.12-0.0001317626-0.0000029203-21.815
5.65-4743444-4.48346.72-0.0001353866-0.0000028978-22.415
5.6533-4862462-4.60247.872-0.0001389804-0.0000029032-23.01
5.6567-5000473-4.7448.576-0.000143148-0.0000029469-23.7
5.66-5083492-4.82349.792-0.0001456546-0.0000029253-24.115
5.6633-5108511-4.84851.008-0.0001464096-0.0000028703-24.24
5.6667-5227520-4.96751.584-0.0001500034-0.0000029079-24.835
5.67-5310542-5.0552.992-0.00015251-0.000002878-25.25
5.6733-5389548-5.12953.376-0.0001548958-0.000002902-25.645
5.6767-5425562-5.16554.272-0.000155983-0.0000028741-25.825
5.68-5475562-5.21554.272-0.000157493-0.0000029019-26.075
5.6833-5492576-5.23255.168-0.0001580064-0.0000028641-26.16
5.6867-5547584-5.28755.68-0.0001596674-0.0000028676-26.435
5.69-5627573-5.36754.976-0.0001620834-0.0000029483-26.835
5.6933-5640590-5.3856.064-0.000162476-0.000002898-26.9
5.6967-5688594-5.42856.32-0.0001639256-0.0000029106-27.14
5.7-5698591-5.43856.128-0.0001642276-0.0000029259-27.19
5.7033-5727604-5.46756.96-0.0001651034-0.0000028986-27.335
5.7067-5737598-5.47756.576-0.0001654054-0.0000029236-27.385
5.71-5721603-5.46156.896-0.0001649222-0.0000028987-27.305
5.7133-5689595-5.42956.384-0.0001639558-0.0000029078-27.145
5.7167-5714592-5.45456.192-0.0001647108-0.0000029312-27.27
5.72-5698597-5.43856.512-0.0001642276-0.0000029061-27.19
5.7233-5669591-5.40956.128-0.0001633518-0.0000029103-27.045
5.7267-5651580-5.39155.424-0.0001628082-0.0000029375-26.955
5.73-5638581-5.37855.488-0.0001624156-0.000002927-26.89
5.7333-5592569-5.33254.72-0.0001610264-0.0000029427-26.66
5.7367-5576571-5.31654.848-0.0001605432-0.0000029271-26.58
5.74-5531558-5.27154.016-0.0001591842-0.000002947-26.355
5.7433-5437556-5.17753.888-0.0001563454-0.0000029013-25.885
5.7467-5348553-5.08853.696-0.0001536576-0.0000028616-25.44
5.75-5306542-5.04652.992-0.0001523892-0.0000028757-25.23
5.7533-5229535-4.96952.544-0.0001500638-0.000002856-24.845
5.7567-5171519-4.91151.52-0.0001483122-0.0000028787-24.555
5.76-5142513-4.88251.136-0.0001474364-0.0000028832-24.41
5.7633-5067494-4.80749.92-0.0001451714-0.0000029081-24.035
5.7667-4996490-4.73649.664-0.0001430272-0.0000028799-23.68
5.77-4918474-4.65848.64-0.0001406716-0.0000028921-23.29
5.7733-4854458-4.59447.616-0.0001387388-0.0000029137-22.97
5.7767-4758452-4.49847.232-0.0001358396-0.000002876-22.49
5.78-4669432-4.40945.952-0.0001331518-0.0000028976-22.045
5.7833-4556421-4.29645.248-0.0001297392-0.0000028673-21.48
5.7867-4443413-4.18344.736-0.0001263266-0.0000028238-20.915
5.79-4343397-4.08343.712-0.0001233066-0.0000028209-20.415
5.7933-4270378-4.0142.496-0.000121102-0.0000028497-20.05
5.7967-4205362-3.94541.472-0.000119139-0.0000028728-19.725
5.8-4157353-3.89740.896-0.0001176894-0.0000028778-19.485
5.8033-4086341-3.82640.128-0.0001155452-0.0000028794-19.13
5.8067-4024323-3.76438.976-0.0001136728-0.0000029165-18.82
5.81-4002312-3.74238.272-0.0001130084-0.0000029528-18.71
5.8133-3887297-3.62737.312-0.0001095354-0.0000029357-18.135
5.8167-3820284-3.5636.48-0.000107512-0.0000029471-17.8
5.82-3727264-3.46735.2-0.0001047034-0.0000029745-17.335
5.8233-3643251-3.38334.368-0.0001021666-0.0000029727-16.915
5.8267-3526246-3.26634.048-0.0000986332-0.0000028969-16.33
5.83-3386226-3.12632.768-0.0000944052-0.000002881-15.63
5.8333-3273207-3.01331.552-0.0000909926-0.0000028839-15.065
5.8367-3135198-2.87530.976-0.000086825-0.000002803-14.375
5.84-3071179-2.81129.76-0.0000848922-0.0000028526-14.055
5.8433-2961169-2.70129.12-0.0000815702-0.0000028012-13.505
5.8467-2858151-2.59827.968-0.0000784596-0.0000028053-12.99
5.85-2780138-2.5227.136-0.000076104-0.0000028045-12.6
5.8533-2720126-2.4626.368-0.000074292-0.0000028175-12.3
5.8567-2695120-2.43525.984-0.000073537-0.0000028301-12.175
5.86-2631103-2.37124.896-0.0000716042-0.0000028761-11.855
5.8633-257088-2.3123.936-0.000069762-0.0000029145-11.55
5.8667-249176-2.23123.168-0.0000673762-0.0000029082-11.155
5.87-243761-2.17722.208-0.0000657454-0.0000029604-10.885
5.8733-237355-2.11321.824-0.0000638126-0.000002924-10.565
5.8767-226647-2.00621.312-0.0000605812-0.0000028426-10.03
5.88-219732-1.93720.352-0.0000584974-0.0000028743-9.685
5.8833-205716-1.79719.328-0.0000542694-0.0000028078-8.985
5.8867-191310-1.65318.944-0.0000499206-0.0000026352-8.265
5.89-1853-1-1.59318.24-0.0000481086-0.0000026375-7.965
5.8933-1790-5-1.5317.984-0.000046206-0.0000025693-7.65
5.8967-1715-14-1.45517.408-0.000043941-0.0000025242-7.275
5.9-1609-35-1.34916.064-0.0000407398-0.0000025361-6.745
5.9033-1547-28-1.28716.512-0.0000388674-0.0000023539-6.435
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