7/28/2019 Chronic Effect of Pferospermu

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each 10 patients that were medicated with oral hypoglucemiants stoppedusing them after the treatment with autohemotherapy with a p < 0.05.Conclusion.The autohemotherapy treatment represents a new, effective method fortreatment of Diabetes Mellittus Type 2, through reduction and normal-ization of the plasma glucose levels, and reduction of the use of oralhypoglucemiants, as well.

P954Chronic Effect of Pferospermum Aceri jol ium Bark on Glycemic andLipidemic Status of Type 2 Diabetic Model RatsS. MURSHED, B. Rokeya, L. Ali, N. Nahar*, A.K. Azad Khan,M. Mosihuzzaman 2. I Research Division, Birdem, Dhaka, Bangladesh:2 Dept of Chemi stry , Dhaha Uni versity , Dhaka, Bangladesh

Pterospermum acerifolium is used as an antidiabetic plant in the Indiansubcontinent, but its hypoglycemic effect has not yet been scientificallystudied. Recently we found antihyperglycemic activity in the leaves ofthis plant in type 2 diabetic model rats. A subfraction (E4b) of the ethanolextract of the bark of the plant has now been tested both for its acute andchronic effects on glycemic status as well as on lipid levels in the samemodel. E4b (1.25 g/kg body weight) was fed daily to rats for 28 days. TheControl rats were fed only with water. For acute experiment, blood sam-ples were collected at 0, 30 and 75 minutes after feeding E4b/water withsimultaneous oral glucose load (2.5 g/kg body weight). Chronic effect ofE4b was evaluated by measuring glucose, fructosamine, triglyceride, totalcholesterol and HDL-cholesterol in the fasting plasma at the beginningand at the end of the study period. The results of the acute test showed thatE4b has significant hypoglycemic effects (sum of increments over basalvalue, MfSEM; Control vs treated: 24.82f2.99 vs 12.45f0.55 mmohl;p