clinical chemistry instrumentation ppt
TRANSCRIPT
INSTRUMENTATION
QUOTE FOR THE DAY
OBJECTIVES• AT THE END OF THE LECTURE, YOU
SHOULD BE ABLE TO:
• 1. EXPLAIN THE PRINCIPLES OF THE METHODS.
• 2. DISCUSS THE FACTORS THAT MAY AFFECT THE METHODS.
• 3. KNOW THE IMPORTANCE OF THESE METHODS TO THE PROFESSION.
COLORIMETRY• VISUAL
• PHOTOELECTRIC
SPECTRONIC 20 D
CELL WELL
ON/OFCONTROL
0 ABSORBANCE CONTROL
ABSORBANCE /TRANSMITTANCE
CONTROL
DISPLAY WINDOWLED DISPLAY
MODEBUTTONSINCREASEDECREASE
CONCENTRATIONBUTTON
WAVELENGTHCONTROL
BEER LAMBERT’S LAW
SPECTROPHOTOMETRY
• BEER LAMBERT’S LAW O.D
Cu
% T
O.D.Cu
% T
Linear Graphing Paper
WHICH ONE IS MORE CONCENTRATED, BASED ON COLOR?
INTENSITY OF COLOR
v-frequency
wl
WAVELENGTHS
Infra red
visible
ultraviolet
Schematic of a single beam spectrophotometer
ELECTROPHORESIS
Gel Electrophoresis
PAPER CHROMATOGRAPHY
Completed Paper Chromatography of a Plant Pigment
ELECTROCHEMISTRY
TURBIDIMETRY
EMISSION FLAME PHOTOMETRY
EFP
ASPIRATOR
ATOMIZER
FLAME
VOLUMETRIC & TITRIMETRIC
GRAVIMETRIC
NEPHELOMETRY
FLUOROMETRY
ATOMIC ABSORPTION SPECTROSCOPY
AAS
AAS
AAS
MASS SPECTROPHOTOMETRY
OSMOMETRY
CHEMILUMINESCENCE
BRING OUT ¼ SHEET OF YELLOW PAPER
POST QUIZ• 1. Method that measures blocked light• 2. Method that applies the separation of
substances using mobile and stationary phase.• 3. Give the relationship of size to electrophoretic
mobility.• 4. In chromatography, what is the stationary
phase?• 5. Give one method that applies the separation of
ions in an electric field.• 6. Range of IR light.• 7. Range of visible light.• 8.-10 Name factors that affect EFP.• 11-15.Trascribe IEC,PC,GC,RBS, FBS
1. Method that measures Absorbed light2. Method that applies the separation of charged particles in an electric field.3. Give the relationship of density to electrophoretic mobility.4. In chromatography, what is the mobile phase?5. Give one method that separates ions/atoms from one another.6. Range of visible light.7. Range of uv light.8.-10 Name factors that affect electrophoresis method