clostridium botulinum - biotecon diagnostics · clostridium botulinum detection lyokit,...

Matrices For matrices like dairy products (e.g. milk powder), meat, fish, canned goods, honey and others Now available! foodproof ® Clostridium botulinum Detection LyoKit, 5’Nuclease 96 reactions Order number: R 602 40 For safer food – BIOTECON Diagnostics – simply builds up trust! Clostridium botulinum is a rod-shaped, anaerobic, spore-forming bacterium capable of inducing foodborne botulism when consumed in contaminated food. From the eight different toxin types A to H, only A, B, E and F are known to cause disease in humans. Improperly preserved foods, such as canned goods or pickled fish, are the most common form of contracting foodborne botulism. C. botulinum is a naturally occurring soil bacterium. Thus, it can potentially contaminate food at multiple stages within the production process, including honey and milk. The foodproof ® Clostridium botulinum Detection LyoKit is based on real-time PCR technology, which is well-established in the food industry as a highly sensitive and specific detection method. The kit detects and identifies all of the human disease- causing toxin types (A, B, E and F) in one multiplex PCR assay using melting curve analysis. This method is a superior alternative to other assays, including mouse bioassay. Fast: Real-time PCR method, based on ISO/TS 17919. Safe: Prevention of false-negative results by internal control and prevention of carry-over contamination using Uracil-N- Glycosylase. Comprehensive: Packages of sample preparation & detection kits, real-time PCR instruments & diagnostic software. Easy: Convenient, complete solution including DNA extraction and real-time PCR analysis. Experienced: Manufacturer of PCR- based rapid tests for the food industry since 1998 with an ISO 17025 accredited service lab. Licensed: Fully licensed technology. Detection LyoKit Clostridium botulinum foodproof ®

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Page 1: Clostridium botulinum - BIOTECON Diagnostics · Clostridium botulinum Detection LyoKit, 5’Nuclease 96 reactions Order number: R 602 40 For safer food – BIOTECON Diagnostics –

Matrices

For matrices like dairy products (e.g. milk powder), meat, fish, canned goods, honey and others

Now available!

foodproof® Clostridium botulinum Detection LyoKit, 5’Nuclease 96 reactions Order number: R 602 40

For safer food – BIOTECON Diagnostics –simply builds up trust!Clostridium botulinum is a rod-shaped, anaerobic, spore-forming bacterium capable

of inducing foodborne botulism when consumed in contaminated food. From the

eight different toxin types A to H, only A, B, E and F are known to cause disease in

humans. Improperly preserved foods, such as canned goods or pickled fish, are the

most common form of contracting foodborne botulism. C. botulinum is a naturally

occurring soil bacterium. Thus, it can potentially contaminate food at multiple stages

within the production process, including honey and milk.

The foodproof ® Clostridium botulinum Detection LyoKit is based on real-time PCR

technology, which is well-established in the food industry as a highly sensitive and

specific detection method. The kit detects and identifies all of the human disease-

causing toxin types (A, B, E and F) in one multiplex PCR assay using melting curve

analysis. This method is a superior alternative to other assays, including mouse

bioassay.

Fast: Real-time PCR method, based on

ISO/TS 17919.

Safe: Prevention of false-negative results

by internal control and prevention of

carry-over contamination using Uracil-N-

Glycosylase.

Comprehensive: Packages of sample

preparation & detection kits, real-time

PCR instruments & diagnostic software.

Easy: Convenient, complete solution

including DNA extraction and real-time

PCR analysis.

Experienced: Manufacturer of PCR-

based rapid tests for the food industry

since 1998 with an ISO 17025 accredited

service lab.

Licensed: Fully licensed technology.

Detection LyoKit

Clostridium botulinum

foodproof®

Instrument Compatibility

5‘Nuclease / TaqMan® cycler (e.g. PikoReal 24, AriaMx, LightCycler 480, LC 96, LC 2.0, ABI 7500)

BIOTECON Diagnostics GmbH

Hermannswerder 17 14473 Potsdam Germany

Phone: +49 (0)331-2300-200 Fax: +49 (0)331-2300-299

[email protected] www.bc-diagnostics.com

DNA Extraction (approx. 30 min)

foodproof® ShortPrep II Kit enables the extraction of Clostridium DNA from food matrix enrichments

Workflow

StartEnrichment

DNA Extractionapprox. 30 min

PCR Setupapprox. 10 min

Real-time PCR Run60 - 110 min

Ana

lysi

s R

esul

ts in

2.5

Storage at 2 °C to 8 °C96 Reactions with a final volume of 25 µl

foodproof® is a registered trademark of BIOTECON Diagnostics.The above mentioned real-time PCR instruments are registered trademarks of their respective holders.

-CB

-01-

V02

/201

Clostridium botulinum Detection LyoKit

R 602 40 foodproof® Clostridium botulinum Detection LyoKit (5‘Nuclease)

Identification of toxin-genes A, B, E and F

RDK 100 23 foodproof® Clostridium Control Detection Kit (5‘Nuclease)

Detection of Clostridium spp. (e.g. C. sporogenes, C. botulinum, C. butyricum) for proficiency testing

DNA Extraction Kits

S 400 02 foodproof® ShortPrep® II Kit - rapid purification

S 400 08 foodproof® StarPrep Two Kit - bulk purification