cmb lab oral report

8/17/2019 CMB Lab Oral Report

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EXPERIMENT 2:

CELL STAINING TECHNIQUES(Lugol’s Solution)

4 Bio 3 Lo amia !ali"s# !a$%l


2/17

Introduction:


3/17

Tetrahymena sp.• "iliat#& singl#'"#ll#&

p$otooan

• $#s*+at#$ o$ganismt*at in*a,itsst$#ams la-#s an&

pon&s• "#lls a$# la$g# 4.'/.

um

• "#lls a$# in#0p#nsi1#

to g$o+• g$o+ $api&l2 to *ig*

nsit2 in a 1a$i#t2 om#&ia an& "on&itions Source:

*ttp+++5oolog25u,"5"a"ou$s#s,io336La,

sCiliat#7$o8#"tt#t$a*2m#naTET9A+#,5*tm


4/17

Tetrahymena sp.•

poss#ss#s man2"o$# p$o"#ss#s"ons#$1#& a"$oss a+i &i1#$sit2 o#u-a$2ot#s(in"lu&ing *umans)t*at a$# not oun&in ot*#$ singl#'"#ll#& mol

s2st#ms

Source:*ttp+++5oolog25u,"5"a"ou$s#s,io336La,

sCiliat#7$o8#"tt#t$a*2m#naTET9A+#,5*tm


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Lugol’s Solution

• %$st ma in :;6u#ous solution o Io&in# an&

7otassium io&in# +it* a p$#"is# $atio o:6

• also $##$$#& to as Io&on#'7otassiumsolution o$ I?I


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Lugol’s Solution

Applications:• o$ t#"tion o sta$"*

• o$ &isin#"tion pu$pos#s

• o$ t*# staining o ,a"t#$ia a""o$&ing toG$am

• an& as a %0ati1#5

• It is also us#& in 1a$ious #0p#$im#nts to

o,s#$1# *o+ a "#ll m#m,$an# us#sosmosis an& &i@usion an& in S"*ill#$st#st in o$$ to &iagnos# "#$1i"al "an"#$5


7/17

Lugol’s Solution

As a fxative:

Lugol’s io&in# is a,l# to -ill "#lls +it*out

&isto$ting t*# st$u"tu$#


8/17

Lugol’s Solution

Detection of Starch• Sta$"* "onsists o am2los# (6.'3.) an&

am2lop#"tin (.';.)5 T*# $atio oam2las#am2lop#"tin 1a$i#s p#n&ing

on t*# natu$al sou$"# o t*# sta$"*5Am2los# is o$m#& ,2 ,$an"*#& an& un',$an"*#& "*ains o glu"os# monom#$s5DI/ ' ions o Lugol’s solution int#$"alat#into sta$"* mol#"ul#s5 Int#$"alation op#nta'io&i $#sults in &i@#$#nt "olo$s ot*# "ompl#0#s am2las# is stain#& in

,lu# am2lop#"tin is stain#& in 1iol#t $#&5


9/17


10/17

Results and Discussion

G


11/17

Group 1Group2

Group 3 Group

G


12/17

Group !Group"

Group # Group $


13/17

Group % Group 1&


14/17

F,s#$1ation

T*# Tetrahymena app#a$#& "ompl#t#l2,la"-,$o+n in t*# s"ann#$ o,8#"ti1# L7FH7F an& FIF5


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'onclusion:


16/17

Con"lusionSin"# Lugol’s io&in# is an in&i"ato$ t#st o$t*# p$#s#n"# o sta$"* it $#a"ts ,2 tu$ning

into a ,la"- o$ &a$- ,lu# "olo$5 It also stopsall t*# "#llula$ un"tions o t*# "#ll5 Lugol’sio&in# -ill#& t*# "#ll *o+#1#$ t*# stain+as a,l# to #nt#$ t*# "#ll an& s*o+ t*#nu"l#i an& gl2"og#n 1a"uol#s ma-ing t*#"#ll app#a$ "ompl#t#l2 ,$o+n in t*#mi"$os"op#5


17/17

The End

4 Bio 3 Lo amia !ali"s# !a$%l

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