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Clarity User TrainingAdvanced

Clarity User Training

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For advanced users

User settings and display options

Sequences

Multidetector systems

Export data

Report setup

Batch data reprocessing

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For advanced users

Data archivation

Column Performance

Noise and Drift

Special calculations

Control modules

GLP a CFR21part11

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User settings are stored in individual users desktop *.dsk

files.For this reason they are set in

the instrument window and not in the main window

User settings

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Text Label and Linesfor legends and arrows

Chromatogram/Create Label

Moving and Scaling of

Chromatograms

Math operation

s

Overlay activation

Labels and display options

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Colored peak area in chromatogram

Peaks in calibration can have a color assigned.

When these peaks are identified in chromatogram they will be

automatically colored

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Colored peak area in chromatogram

Highlight currently selected peaks

Simply select a peak in the chromatogram result table.

The result table selection overrides the color defined in calibration

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Colored peak area in chromatogram

Highlight peaks selected in Results table (signal color used)

Use Peak Color set in Calibration file

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Context menu invoked by right mouse click in table

Table customizing options

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Common columns in Summary table

Independent setting of columns Common - for all compounds

Summary – compound specific

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Summary table options

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Context menu invoked by right mouse click in

Chromatogram window

Axis properties setting

Graph properties

setting

Graph display options

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Gradient display options

Setting for individual

signals

Chromatogram display options

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Customizing Toolbars

Reset Allrestores original

settings

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Define a serie of samples to be measured

Essential for working with autosamplers For controled autosamplers the Vial number and Injection

volume are used from this table

Allows modifications during a run for not yet processed samples Simply add samples by filling any field in the last empty row.

Other fields will be copied from previous. Several samples can be added by copying from a table

Allows for import of sample data from text files

Allows reprocessing of last measured sample set

Sequence table

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Start, End vial and Injections number. Several vials and/or

injections defined on single row

File name, automatic

%variables for SV, EV, IV

Automatic recalibration

from sequence

Row status indicator

Use row, add new rows during

sequence run

Tooltip with actual file name

Individual Post-Run

settings for each row

Sequence table window

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Pasive sequenceClarity only expects Start signal,

analysis time is set on autosamplerActive sequence

Clarity sends a Ready signal to autosampler and waits for Start

signalAS control (Active sequence)

Autosampler injects according to SV, EV, IV and

Inj. Volume in sequence table

Editation of template method used on

selected row

Initial value for %n

Sequence options

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Run sequencestarts complete sequence

(resets the status)

Pause sequence

Resume sequencecontinues from last

injection

Status of the sequence. Stored at Pause, Abort, Stop

Can be changed by Edit/Reset Status for selected rows

Repeat Injection

Skip Vial

Stop sequence will stop the sequence run. Repeated Stop will stop the current

analysis

Sequence control

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Select the file to import

Assign the respective fields to be importedOnly Start vial (SV) and file name must be

set (marked in bold)Other fields will be filled by default values

Sequence import

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Export data to other programs to prevent typing errors Simple Copy and Paste (Ctrl C – Ctrl V)

Export Data settings common for manual export from chromatogram window

automatic export from postrun at the end of each run

automatic export by batch reprocess of chromatograms or sequence

Export of chromatograms in common formats

Export data

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Copy a picture to MS Office (including labels) via Clipboard or store it

in *.emf format

Copy a selected part of table using Copy (Ctrl C) and Paste (Ctrl V)

Export data - simplest

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Headers:Column headers and chromatogram info

Full format:Filename, date and time

before each row

blank field = actual chromatogram name + .txt extensionPath can be used

All exported data are in single file

Export time/signal data pairs

(import to other programs – for

example graphics)

Export data:manual - from Chromatogram window

automatic - according to Setting/Postrunbatch - from Analysis/Batch

Export data - options

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Export data - example

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print

Report style from Report Setup dialog will be used

do not forget to switch off the Overlay mode

export

reprocess of chromatograms according to instrument method

reprocess whole sequence

electronic signature

File typeChromatograms

Calibration standardsSequence Files

Export according to Settings - Export Data

Electronic signature for series of chromatograms

Batch processing

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Assignment of detectors to Clarity Instruments in the System Configuration dialog

All signals stored in a single chromatogram Result table, Integration table and Acquisition

parameters are individual for each chromatogram

Common calibration file calibration curves are individual for each signal

Summary table for display of results from all signals

Multidetector systems

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Results are displayed individually, some properties like calibration are

common for the whole chromatogram

Connecting link marks signals from one chromatogram. Click icon to set signal

active

Detector Delay compensates for

interdetector volume, inactive for first signal

Multi signal chromatograms

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Summary table displays results for several chromatograms or

signals simultaneously

In overlay, several chromatograms can be

compared. Maximum number can be set in user options

Doubleclick on legend will set signal active

Scale, move and math

operations are active only in

overlay

Ctrl + click will hide/show the signal

Ctrl + click will lock the tool for repe-

titive use.

Overlay

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Report Setup - logo

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Report Setup – tiled signals

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To reduce the data size compressed archive *.dgz files should be used

*.dgz archives always replace all data (files deleted from project will be during archiving deleted also from the archive).

It is advantageous to archive whole projects – should be considered when planning project structure

Archiving and backup

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Archive and Restore for copying or moving projects Selected file types Without compressing = original format and structure compressed *.dgz archives = single file

Archive data

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Separation parameters

Values used for Capacity ratio and Efficiency calculation

Calculation type

Performance tab (hidden in

default layout)

Advanced tab in the Method Setup (displays

template method

Performance parameters

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Actual noise monitoring in the Data Acquisition window

Noise monitoring

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Drift and Noise displayed for selected intervals in

Result table header

Drift and Noise can be used for in User column calculations

Noise and Drift

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ISTD calculations

Normalized % calculations

Groups calibration of peak group like single compound example - isomers (xylen)

Scale factor recalculation of result to other units

Uncalibrated response quantification of unidentified peaks using Uncalibrated

response factor

Injection volume Injected volume correction for manual injection in GC

Calculations

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ISTD calculations are performed:a) Amount for ISTD is set in both calibration and sampleb) both are zero

ISTD compound is calibrated

ISTD amount in sample is given

Only one ISTD compound is allowed in calibration. If the conditions are not

met, no results will be calculated

ISTD peak can be hidden in the Result table

ISTD calculations

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If Sample amount is non-zero, it will replace the sum in the Amount column and serve as a base for Amount

% calculation

Total line is calculated as a

sum of all groups + sum of peaks not belonging to

any group. Groups are

calibrated, thus peaks should not be both calibrated and in a group to

get correct Amount% values

Amount % gives the normalized % if all

integrated peaks are calibrated and Sample

amount is zero

Normalized % calculations

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1st stepAdding peaks to a group in the integration table

2nd stepSetting a Group in Calibration table.

The compound name and amount is set as for

ordinary peaks

Groups

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Scale factor and Units after scaling:Multiplies values in Amount column and

changes the units in column header.Method specific parameter, multiplies also

the entered Sample Amount value

Uncal. Response:Used for quantification of

unidentified compounds. For universal response detectors

(ELSD, FID, RI)

Dilution:Multiplies values in Amount column.

Sample specific parameter (entered from Sequence or Single Analysis window).

Scale Factor and Uncal.Response

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Default Injection Volume must be set first in calibration and Injection Volume set in standards before calibrating

Supposes linear detector response

Can lead to incorrect results, if used improperly

Setting Default

Injection Volume in calibration

Setting Injection Volume for sample and

standard

Injection Volume

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Command Calibration/Show History displays

data used for selected level

Deviation of point from calibration curve in %

Residual sum of deviation squares

Calibration

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Calibration Retention (Kovats) indexes

LOD and LOQ limitsColumns for Retention (Kovats) indexes are

hidden in default settings.

Use setup columns to disdplay them

Amounts lower then specified LOD or LOQ limits are marked in Peak type column

Retention indexes, LOD, LOQ

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For selected instruments the direct control is available. The advantages are

The instrument parameters are stored in a method

It is not necessary to enter the parameters twice, especially useful for autosamplers

The parameters are recorded in the chromatogram

Control Modules

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Control modules enable full control of connected devices like gradient programe and

other settings. The used settings are stored within a

chromatogram.

Control modules (GC)

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Control modules (LC, AS)

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Option to Send/Confirm/Do not send method to instruments after method change

Send Method options

Confirmation dialog

Control Modules

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Control modules overview

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Other devices can be controlled by A/D board digital outputs: gradient or detector program start detector autozero valve switching fraction collector control

Synchronization in Active Sequence Dig. output set in System Configuration, not in the Event table

Additional control

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Set events in the Event Table tab in the Method Setup

Named events

Digital event input type

Output event can trigger run/sequence commands: Stop Abort Skip vial Shutdown Repeat injection

Event table

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Chromatogram history All saved versions are stored in the chromatogram

Versions can be opened (including calibration)

Audit trails Station, Chromatogram, Sequence, Calibration

User access Can be restricted by User Accounts settings

GLP a CFR21 part 11

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Electronic signatures based on User Accounts

based on Certificates

Validation Installation qualification - standard feature

Performance qualification - by optional Validation kit

Operation qualification - SST module for statistical evaluation and pre-set limits control

GLP a CFR21 part 11