Slide 1

COL Art Lyons, PhD,MD February 26, 2014 Fort Riley Trop. Med. Course Malaria Lab Session

Slide 2

Helpful Reference: WHO Health Library for Disasters http://helid.digicollection.org/en Search for malaria Basic Malaria Microscopy Learners Guide http://whqlibdoc.who.int/publicati ons/2010/9789241547826_eng.pdf

Slide 3

Slide 4

Slide 5

Blood Smear for malaria Thick Film Thin Film Red blood cells layered 10-20 times thicker v. thin film Red Cells are not fixed (staining caused lysis) thus the parasites are within red cell ghosts Can evaluate large amount of red blood cells Sensitive (negative more likely to rule out malaria) Single layer of red blood cells Fixed with methanol Used to identify parasite species. Used to estimate parasitemia View after thick smear Specific STAINING: http://www.dpd.cdc.gov/dpdx/HTML/PDF_Files/malaria_staining_benchaid.pdf

Slide 6

Chromatic = red Cytoplasm = blue Dont see both = Not malaria

Slide 7

Slide 8

Key Morphological Differences Between Human Plasmodium Species in Blood Smears P. falciparumP. vivaxP. ovaleP. malariae numerous rings smaller rings NO trophozoites or schizonts seen cresent-shaped gametocytes enlarged erythrocyte (younger cells) Schffner's dots diffuse and fine 'ameboid' trophozoite similar to P. vivax compact trophozoite fewer merozoites in schizont Elongated, erythrocyte compact parasite merozoites in rosette

Slide 9

Slide 10

Slide 11

Slide 12

P. falciparum

Slide 13

P. vivax Giemsa Schuffners dots

Slide 14

P. ovale Coarse Pigment Compact troph.

Slide 15

P. malariae Unlike Vivax, Not Enlarged

Slide 16

The End