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Phone: 888-EASYGELFax: 574-533-3370Web: www.micrologylabs.comEmail: info@micrologylabs.com

Selective For: Sorbitol positive E. coli and other coliforms, and Sorbitolnegative, glucuronidase negative E. coli.

Description: A pink medium for the identification and differentiation ofbeta glucuronidase positive, sorbitol positive strains of E.coli and glucuronidase negative, sorbitol negativestrains of E. coli. Other coliforms which are sorbitolpositive will also be identified.

Composition: Contains nutrients, a chromogenic enzyme substratemix, the sugar sorbitol, and a pH indicator. pH 7.8 ± 0.2

Procedure: Use standard methodology for product being tested.

Incubation time and temperature;20-30 hrs at 35ºC.

Crowded colonies (CFUs) closer than 3-4 mm on thedish may result in difficult to interpret background zones(color).

Interpretation: Standard glucuronidase positive, sorbitol positive E. coliCFUs are dark blue/purple surrounded by a yellow zone. Other coliforms (galactosidase positive, sorbitolpositive) are red surrounded by a yellow zone.Glucuronidase negative, sorbitol negative E. coli(includes 0157:H7) CFUs are red without a surroundingzone. It is common that both the standard E. coli andthe sorbitol negative E. coli colonies are larger than thecolonies of the other coliform types.

Storage: Freeze

Shelf Life: 1 year

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Micrology Laboratories, LLC.PO Box 340Goshen, IN 46527-0340 USA