Labib Bakkali, Stéphan Zientara, Pascal Boireau

ANSES

Animal Health Laboratory

Maisons-Alfort Email: pascal.boireau@anses.fr

Collaboration between

IRVT- OIE RL Brescia- Anses

Maisons-Alfort Animal Health

Laboratory

www.anses.fr

Animal Health Laboratory

Created in1901

Emile Roux

Bull.soc.fr.hist.méd.sci.vét., 2006, 6 : 79-107

85

virulentes, premiers vaccins à base de

d’aphtes ou de tissus de cobayes

neutralisés (Carré , Rinjard et Vallée : leurs

travaux ouvriront la voie au vaccin de

Schmidt et Waldmann ), hémo-prévention

(Rinjard), culture in vivo sur divers tissus

de fœtus de bovin (Thomas, Dhennin) ou

sur lapin nouveau-né (Verge, Dhennin,

Asso), vaccination par voie intradermique (

L.et L. Dhennin, Paraf, Larenaudie),

absence d’immunité croisée entre virus

OAC et SAT 1 (Santucci et coll.),

diagnostic et épidémiosurveillance

(Goureau, Kaiser, Moutou)

Fièvre charbonneuse : diagnostic,

surveillance, contrôle (Vaissaire). Fièvre catarrhale ovine et Fièvre du Nil occidental : surveillance de ces deux

maladies, diagnostic et surveillance de la

maladie, enquêtes sérologiques évaluation

des vaccins (Zientara)

Germes pyogènes : production de sérums

neutralisant spécifiques (Leclainche et

Vallée)

Immunologie : tolérance immunitaire

(Verge, Paraf, Goret), diagnostic

sérologique de la distomatose (Luffau et

coll.)

Maladie du jeune âge du chien : découverte du virus causal par Carré

(figure 2), dont le nom restera attaché à

cette maladie. Démonstration du rôle

majeur des « infections secondaires » dans

les maladies à virus.

Maladies des ovins : étude de l’agent

causal de la maladie caséeuse (Bacille de

Preisz-Nocard) et premiers essais de

vaccination contre cette maladie (Carré,

Nocard). Isolement du virus de

l’avortement de la brebis (Dhennin,

Gourdon, Quinchon…)

Maladie des chevaux : anémie infectieuse

du cheval étiologie, matières virulentes,

porteurs sains, règles de la prophylaxie

sanitaire (Vallée et Carré), premier

Figure 2 : Henri Carré dans son

laboratoire

diagnostic de l’avortement à virus de la

jument (Lucas et col.), surveillance des

maladies bactériennes, parasitaires et

virales (Plateau, Zientara), dont la

babesiose équine, la grippe équine, la

maladie de Borna etc..

Maladies des porcins : premiers

diagnostics en France de peste porcine

classique (Lucas et coll.) puis de peste

porcine africaine (Carnero, Crucières,

Haag, Lacaze, Larenaudie,),

Maladies aviaires : découverte de la

trichomonose intestinale du faisan, de la

maladie de Newcastle de la perdrix, de la

teigne du dindon à Trichophyton (Lucas et

coll.), démonstration de réactions

antigéniques croisées entre Salmonella pullorum et d’autres entérobactéries

(Gaumont) : diagnostic, surveillance et

contrôle de la Chlamydophilose aviaire

(Vaissaire) Maladies des animaux sauvages : diagnostic, surveillance et contrôle,

Henri Carré Edmond Nocard (1900)

REFERENCE MISSIONS Agrement Agriculture ministry, Health ministry, DG SANCO, OIE, FAO

2 European Reference laboratories Brucellosis – Equine diseases

OIE reference laboratory - FAO Collaborative center Brucellosis | Tuberculosis | EHDV

OIE Collaborative center Food borne parasites

Human reference center

Brucellosis

REFERENCE MISSIONS Agrement Agriculture ministry

2 LABORATOIRES

DE REFERENCE COMMUNAUTAIRE Brucellose – Maladies équines

LABORATOIRE DE RÉFÉFENCE OIE - CENTRE

COLLABORATEUR FAO Brucellose | Tuberculose | Paratuberculose

Centre national de référence

Brucellose

Centre national de référence associé

Charbon bactéridien

15 national mandates Anthrax, Chlamydiosis Brucellosis and tularemia Tuberculosis FMD Bluetongue and EHDV West Nile Swine vesicular disease Viral horse diseases and glanders, dourine (with DOZULÉ Laboratory) Parasite transmitted by food Trichinellosis, Protozoa, Anisakis, (arthropod (ticks and phlebotomes) borne diseases (Bartonella, Anaplasma, Leishmania…)) Schmallenberg virus, Bunyavirus

FMDV C3 lab

Campus Alfort

CRBM

New lab C3FMDV, tick

borne diseases « I3 »

Networks REMESA and

RESOLAB

Training and international cooperation on

FMDV

Training and technology transfert

Research project for the

development of new tools

- 2 training weeks

- Providing Reagents

Marocco Tunisia Algéria

Bilateral actions on FMDV

Maisons-Alfort

18/10/10 to 29/10/10

Mustapha

HAMMOUCHI

Soufien

SGHAIER

Nadera

CHAOUACH

ANSES – Laboratoire de Santé Animale, Maisons-Alfort, France

21 – 25 May 2012

Tchad

Algérie

Niger Tunisie Mauritanie FAO

Liban Pakistan

Bénin

Workshop and training for FMDV diagnosis tests.

Vet laboratory ACCRA (Ghana)

17 – 21 SEPTEMBER 2012

Training seminar Accra

CAMEROON,

Dr. Simon Dickmu Jumbo

CENTRAL AFRICA REPUBLIC

Dr. Marie-Noel Ouaimon

COTE D’IVOIRE

Dr. Gnabro H. P. Godji

D. R.CONGO

Dr. Leopold Mulumba

GHANA

Dr. J. A. AWUNI

NIGERIA

Dr. Hussaini G. Ularamu

REPUBLIC OF CONGO

Dr. Ngakeni Faramalala

SENEGAL

Dr. Papa Serigne SECK

TOGO

Mr. Felix Kodzo WOUME

Kees van Maanen Labib Bakkali Kassimi

Training seminar on FMDV ACCRA: practical traning

Training for direct

indirect tests

LFD

Ag-ELISA & NSP ELISA

RT-PCR

Workshop on epidemiological surveillance of FMD and the

capacity of the laboratory

Dakar, 3-4 June 2014

Mali, Mauritania and Senegal

Project of common

epidemiological surveillance

among the three countries

Isolation and characterisation of FMDV strains

Benin

Type O

Type A

PhD Ghana

Type O

Nigeria

SAT 2

Cameroon

SAT 2

→ University of Liege, Belgium

→ University of Abomey-Calavi

in Cotonou, Benin

→ Belgian Development

Collaboration Department

Institute of Tropical

Medicine (Antwerp)

Accra Veterinary

Laboratory,

Ghana

Collaborations

Gorna et al.

Vet.Microbiol. 2014

FMD Research Centre,

National Veterinary

Research Institute,

Nigeria

Laboratoire

National

Vétérinaire

Cameroun

Molecular epidemiology

Maisons-Alfort

16 juin 2014 au 27 juin 2014

Amina AQARI Nadia CHAFFAI

Biopharma

Rabat Marocco

Bilateral actions on FMDV

- 2 weeks for training on emerging

outbreak

- To provide reagents

- Practical training in Tabat between

29/9 and 3/10/2014

Practical training in the field for FMDV

Nakuru, Kenya 25-29 August 2014

Stephan

ZIENTARA

Support actions to REMESA for FMD

Workshops and Training

Bilateral action program of French

ministry of agriculture:

Welcome trainees

Training in countries by experts

Actions supported by EuFMD

Organization of workshop at

Maisons-Alfort

Participation to real time training

Support actions to Tunisia for FMD

Collaboration project between IRVT, OIE RL Brescia and Anses

Genetic and antigenic characterization of FMDV

strains circulating in Tunisia

IRVT IZSLER

Funded by:

French Ministry of Agriculture, Food and Forestry

Agency for the development of international cooperation in the areas of agriculture, food

and rural space (Adecia)

Instituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER)

Context

FMD was introduced in

Tunisia in April 2014

First case was reported in

North East of country

Then, different outbreaks were

reported in different region

and in a short time

Virus identified : serotype O of

topotype Ind-2001d

29/4/2014 13/10/2014

WRLFMD

Objective

Investigation of FMDV evolution during the outbreak

Use of genome sequence and antigenic profiling data

to study:

dynamic of FMDV transmission

the virus matching with vaccine strains

Identify risk factors for the spread of the

disease in the country

Planned activities

Action Month

IRVT will send samples to Anses 1

Welcome trainee from IRVT at Anses for 3 weeks 2

Analysis of samples by trainee : virus isolation, RT-PCR, typing

2 et 3

Genome amplification of selected samples at Anses 4 et 5

Send isolats and amplicons to IZSLER 5

Full genome sequencing from amplicons by IZSLER 6 à 9

Antigen profiling of isolats by IZSLER 6 à 9

Data analysis 10-11

Repport 12

Complete sequences of isolates of FMDV in Tunisia

Phylogenetic analysis of sequences

Antigenic characterization of isolates

Analysis of epidemiological links between isolates based

on phylogenetic analyzes

Analysis of antigenic homology between the vaccine

strains and isolates

Output

Conclusion

Global control of FMD needs coordinated action at

national, regional and international level

Collaborations between veterinary services and

laboratories at regional and international levels are

essential for the success of the control of FMD

Anses participates and will continue to participate actively

in the global fight against FMD

In particular Anses-Animal health Lab, continues to

provide support to REMESA members through training,

expertise and collaboration project

Collaboration projects such as that between IRVT, IZSLER

and Anses should be encouraged

Thank you for your attention

“Unity makes strength”

New diagnostic tools

Improve the existing tests

Multiplex RT-PCR in one step for West Africa

advantages:

Detection and typing of four serotypes in one reaction

Internal amplification control

Cost

IRES/β-actine

Or

3D/β-actine

20µl 5µl

Duplex RT-PCR in one step.

advantages:

Simple (faster)

Less handling

Less risk of contamination

sensitive

Internal amplification control

Stability Mastermix

Pen-Side test

Inactivated

virus

Extraction

ARN

RT-PCR Transfection

Virus

advantages:

Less risk

Shipping Costs

Alternative way for sample sendings

Research project

Ensemble pour mieux comprendre

Identification and characterization of

circulating strains in West Africa