Procedure1.Physical State, Color and

OdorThe physical states must observe at room temperature. The color and odor must also be noted.

2. Solubility PropertiesThe sample will be introduced in

a clean and dry test tube.

IF THE SAMPLE IS

Liquid4 drops of each

sample will be added

Solid0.1 g of sample will

be added

The solid

samples must

be grinded

to increase the

surface

The solvent was added drop wise

and counted the number of drops of solvent

added to a total of

3. Reaction with Litmus PaperIf the sample is soluble in water, test its

aqueous solution with red and blue litmus papers. Note down the color

changes in both litmus papers.

4. Ignition Test -3-5 drops of the liquid sample and a pinch amount if solid will be placed in an evaporating dish and will be lighted using a match.

Note the color of the flame produced If flammable observe burning time

5. Infrared (IR) AnalysisUsed the appendix notes to identify the type of principal bond present in the sample compound that may be observed in the IR spectra and its

wave number range.

Use a dry and calibrated dropper (X number of drops = 1mL). Do not heat

the mixture.

Experiment No. 5Column and Thin Layer

Chromatography

Extract the pigments of the red siling labuyo using DCM-hexane (1:1) and the

malunggay pigments using hexane: acetone (7-3). Set aside a portion of

the extracts for TLC.

Plug the column with cotton and uniformly pack with silica gel up to the indented part of the Pasteur pipette. Place 0.5mL of the extract on top of the column using a Pasteur pipette.

Discard the colorless eluate. Collect the colored eluates in separate vials. Note the number of drops of eluate collected in each vial.Apply the eluates on a 5 cm x 8 cm precoated TLC plate by spotting 10 times. Allow each spot to dry before applying the next. Make sure the spots are as small as possible.

Elute the pigment mixture using 10mL of DCM- hexane (1:1) for red siling labuyo and hexane- acetone

(7:3) for malunggay leaves. Introduce the solvent system in

portions. Do not allow the column to run dry.

Spotting Of Chromatography

plate

Prepare the developing chamber by placing an approximate amount of the solvent system: DCM- hexane (1:1) for red siling labuyo and hexane- acetone (7:3) for malunggay leaves. Line the inner wall of the chamber with filter paper, cover with a watch glass, and allow equilibrating.

Carefully place the plates in the developing chamber (see Figure 5a). Allow the solvent system to rise up to 1cm from the upper end. Remove the plates from the chamber, immediately mark the solvent front, and air- dry.

Visualize the components

using a UV lamp. Measure the Rf

values. Document the

chromatoplates.