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Mitochondrial DNA, Early Online: 1–3! 2013 Informa UK Ltd. DOI: 10.3109/19401736.2013.825789

MITOGENOME ANNOUNCEMENTS

Complete mitochondrial genome of the Amur weatherfish, Misgurnusmohoity (Teleostei: Cypriniformes: Cobitididae)

Yong-Yao Yu, Wen Song, Yi-Zhou Wang, Wei-Min Wang, and Xiao-Yun Zhou

College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture

Collaborative Innovation Center of Hubei Province, Wuhan, Hubei, P. R. China

Abstract

The complete mitochondrial genome of the Amur weatherfish, Misgurnus mohoity is a circularmolecule of 16,566 bp in size, containing 13 protein-coding genes, 22 transfer RNA (tRNA)genes, 2 ribosomal RNA (rRNA) genes, and 2 main non-coding regions (the control region andthe origin of the light strand replication). Most of the genes are encoded on the heavy strand,except for ND6 and eight tRNAs. The control region is 915 bp in length and located betweenthe tRNAPro and tRNAPhe genes, some typical conserved elements (TAS, CSB1-3 and CSB D-F)were found in this region. All these features reflect a typical vertebrate mitochondrial genearrangement of the M. mohoity.

Keywords

Cobitinae, Misgurnus mohoity, mitogenome

History

Received 13 July 2013Accepted 13 July 2013Published online 18 September 2013

The Amur weatherfish, Misgurnus mohoity, an endemic fresh-water fish of East Asia, is restricted to the Amur River drainage(Vasil’eva et al., 2003). M. mohoity is an important aquaculturespecies in China, but only fewer researches have been focus onthis fish. In this study, we report the complete mitochondrialgenome sequence of the M. mohoity (GenBank AccessionNumber: KF386025).

The gene arrangement, base composition, and codon usagepattern of the M. mohoity mitochondrial genome are similar tothose of other teleosts (Broughton et al., 2001; Cui et al., 2009;

Miya & Nishida, 1999; Wang et al., 2013). The completemitochondrial genome (16,566 bp) containing 13 protein-codinggenes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA(rRNA) genes, and 2 main non-coding regions. Most of thegenes are encoded on the heavy strand, except for ND6 and eighttRNAs (Table 1). The overall base composition of the H strandis A: 29.17%, T: 27.38%, C: 26.68%, and G: 16.76%, which isconsistent with the lowest frequency for G among the four bases(Li et al., 2013; Meyer, 1993) and an AþT rich pattern of thevertebrate mitochondrial genomes (Mayfield & McKenna,

Correspondence: Xiao-Yun Zhou, College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong AgriculturalUniversity; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, P.R. China. Tel: +86 027 87282113.Fax: +86 027 87282114. E-mail: zhouxy@mail.hzau.edu.cn

Table 1. Characteristics of the mitochondrial genome of the Misgurnus mohoity.

Position Codon

Gene From To Size (bp) Start Stopa Intergenic nucleotidesb Strandc

tRNAPhe 1 69 69 0 H12S rRNA 70 1021 952 0 HtRNAVal 1022 1093 72 0 H16S rRNA 1094 2768 1675 0 HtRNALeu(UUR) 2769 2843 75 þ1 HND1 2845 3819 975 ATG TAG þ5 HtRNAIle 3825 3896 72 �2 HtRNAGln 3965 3895 71 þ1 LtRNAMet 3967 4035 69 0 HND2 4036 5082 1047 ATG TAG �2 HtRNATrp 5081 5150 70 þ1 HtRNAAla 5220 5152 69 þ1 LtRNAAsn 5294 5222 73 0 L

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1978). Twelve of the 13 protein-coding genes initiate withthe traditional ATG start codon except for COX1, which beginswith GTG instead. Correspondingly, the termination codons of13 protein-coding genes include six TAA and two TAG (ND1and ND2), whereas other five genes (COX2, COX3, ND3, ND4,and Cytb) have the incomplete stop codons T– or TA–. Threenotable ‘‘over-laps’’ between protein-coding genes in the samestrand are found: ATPase 8 overlaps with ATPase 6 for 10 bp,ND4L overlaps with ND4 for 7 bp, and ND5 overlaps with ND6for 5 bp. The 12S and 16S ribosomal RNA genes are 952 bpand 1675 bp long, respectively. Of the 22 tRNA genes, 21 couldbe folded into the classic clover-leaf secondary structure bytRNAscan-SE1.2.1 (Lowe & Eddy, 1997), while the tRNA forserine with the anticodon AGY (tRNASer(AGY)) lacks a DHUarm, which is a common finding in all vertebrates (Kumazawa& Nishida, 1993). Non-coding region of M. mohoity mitochon-drial genome contains the origin of light strand replication(oriL) and the control origin. The oriL is 30 bp in length andlocated in the WANCY cluster region between the tRNAAsn andtRNACys. The control region is a 915 bp sequence bound bytRNAPro and tRNAPhe. Besides being the most variable region,the control region also contains certain conserved motifs that areassociated with the initiation of DNA replication and transcrip-tion (Zhao et al., 2006). The consensus sequence of terminationassociation sequence (TAS) in most fishes (TACAT—ATGTATAATCACC; Cheng et al., 2012) was identified atthe 50 end of the control region. The central conserved-blockdomains, CSB-F (15,885–15,907 bp); CSB-E (15,931–15,949 bp)and CSB-D (15,971–15,992 bp), as well as the conservedsequence blocks, CSB1 (16,205–16,230 bp); CSB2 (16,322–16,338 bp) and CSB3 (16,366–16,384 bp) were also detected. Allthese features reflect a typical vertebrate mitochondrial genearrangement of the M. mohoity.

Declaration of interest

This research was supported by the National Natural Science Foundationof China (Program No. 31001103) and the Fundamental Research Fundsfor the Central Universities (Program No. 2011PY137). This research isalso a component of the Ph.D. Programs Foundation of Ministry ofEducation of China (Program No. 20100146120012). The authors reportno conflicts of interest. The authors alone are responsible for the contentand writing of article.

References

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Table 1. Continued

Position Codon

Gene From To Size (bp) Start Stopa Intergenic nucleotidesb Strandc

OriL 5324 5295 30 0 LtRNACys 5390 5325 66 0 LtRNATyr 5459 5391 69 þ1 LCOX1 5461 7011 1551 GTG TAA þ1 HtRNASer(UCN) 7083 7013 71 þ2 LtRNAAsp 7086 7157 72 þ13 HCOX2 7171 7861 691 ATG T– 0 HtRNALys 7862 7937 76 þ1 HATPase8 7939 8106 168 ATG TAA �10 HATPase6 8097 8780 684 ATG TAA �1 HCOX3 8780 9563 784 ATG T– 0 HtRNAGly 9564 9636 73 0 HND3 9637 9986 350 ATG TA– 0 HtRNAArg 9987 10,056 70 0 HND4L 10,057 10,353 297 ATG TAA �7 HND4 10,347 11,727 1381 ATG T– 0 HtRNAHis 11,728 11,796 69 0 HtRNASer(AGY) 11,797 11,864 68 þ1 HtRNALeu(CUN) 11,866 11,938 73 0 HND5 11,939 13,778 1842 ATG TAA �5 HND6 14,295 13,774 522 ATG TAA 0 LtRNAGlu 14,364 14,296 69 þ6 LCytb 14,371 15,511 1141 ATG T– 0 HtRNAThr 15,512 15,583 72 �2 HtRNAPro 15,651 15,582 70 0 LD-loop 15,652 16,566 915 H

aT– and TA– represent incomplete stop codons.bNumbers correspond to the nucleotides separating adjacent genes, negative numbers indicate overlapping

nucleotides.cH and L indicate genes transcribed on the heavy and light strand, respectively.

2 Y.-Y. Yu et al. Mitochondrial DNA, Early Online: 1–3

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DOI: 10.3109/19401736.2013.825789 Complete mitochondrial genome of the Amur weatherfish, Misgurnus mohoity 3

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