complex i
TRANSCRIPT
Complex I: In the inner mitochondrial membrane, Nicotinamide adenine dinucleotide
(NADH) produced by glycolysis is oxidized (removes electrons) by the enzyme NADH
dehydrogenase. The enzyme removes two electrons from NADH and attaches them to an
electron carrier, ubiquinone. The transfer of these electrons reduces (adds electrons)
ubiquinone into ubiquinol. While this redox reaction is occuring, four hydrogen atoms
(protons) are pumped across the inner membrane to the intermembrane space. This
creates a proton gradient, which basically means there is a higher concentration of
protons outside the inner membrane (in the intermembrane space) than inside the
membrane (in the mitochondrial matrix). NADH binds to Flavin mononucleotide, reducing
NADH to NAD+ and reducing Flavin mononucleotide to FMNH2. Notice that NADH is
losing its negative hydrogen atom, resulting in the positive charge of NAD+. The two
electrons and two hydrogens taken from NADH are carried by FMNH2 (which is now
called an "electron carrier") to two Iron (Fe) atoms in Iron-Sulfur (Fe-S) centers located
within the complex. The hydrogens then act as protons and are pumped back into the
mitochondrial matrix, not the intermembrane space. The electrons in the two irons are
accompanied by two protons and transfered to ubiquinone (remember from the
begining?), which is also called "coenzyme Q." Ubiquinone then passes the electrons to a
new Fe-S center, releasing the two protons into the matrix. A new ubiquinone is given the
electrons and rests within the inner membrane, again pushing two protons to the matrix.
Complex II: Two electrons from the citric acid cycle are transfered to complex II,
powering the oxidation of the enzyme succinate (also from the citric acid cycle) into
fumarate. Fumarate then passes the two electrons to coenzyme FAD, which moves the
electrons to an Fe-S complex and then to ubiquinone. Complex II does not produce a
proton gradient because there is not enough free energy to pump protons into the
intermembrane space.
Complex III: Complex III recieves two electrons from the reduced ubiquinone from
complex's I and II. The electrons are passed through an Fe-S complex to cytochrome C,
an electron carrier, pumping four protons into the intermembrane space, two from
ubiquinone and two from cytochrome C. This creates another proton gradient.
Complex IV: Cytochrome C, which operates in the intermembrane space, transports one
electron at a time to complex IV. These electrons provide the energy needed to reduce
molecular oxygen to two molecules of water. Complex IV creates a proton gradient.