confidential document : access to approved users only cavinkare research centre cavinkare pvt. ltd....

Confidential Document : Access to Approved Users Only

CavinKare Research CentreCavinKare Research CentreCavinKare Pvt. Ltd.CavinKare Pvt. Ltd.

PresentationPresentation - R&D - R&DPresentationPresentation - R&D - R&D



Objectives of R&DObjectives of R&D

• To make R&D a self-supporting / profit making activity by creating and developing Intellectual Property through out-licensing or national / international collaborations

• To contribute to the business objectives of CKPL by developing innovative, cost effective and superior products and processes


Pickles Chinni’s, Ruchi

Spices Chinni’s

Vermicelli Chinni’s

Sweets RuchiDry Fruits Chinni’s

Moments Snack Bar Chinni’s

Cruncho

Foods : Ready-to-eat

Personal Care : Hair, Skin and Home

Shampoo Chik, Nyle, Meera, KarthikaHair Wash Powder Meera, KarthikaHair Dye IndicaHair Oil Meera, NyleCream / Lotion Fairever, FE Mantra, Nyle CC & MLTalc, Deo & Dab-on SpinzToilet Bowl Cleaner TexMosquito Coil Dishoom

Product PortfolioProduct Portfolio


R&D - RelationshipsR&D - Relationships

Salon Business (Trends in Vogue)

International Business

Personal Care (Domestic Business)

Foods (Domestic Business)


• Discovery of new ingredients (exclusivity; superior performance)

• Developing new / improved processes (cost effectiveness)

• Developing new technology (exclusivity; superior performance)

• Strengthening biology (developing capability; studying new pathways)

• Leveraging IPR (drafting claims; analysis; competitive intelligence)

• Superior Formulation (sensory experience; delivery of benefit; care)

R&D Strategy for Competitive AdvantageR&D Strategy for Competitive Advantage

To Provide Superior Products at Affordable Price Points


R&D Set-up & FacilitiesR&D Set-up & Facilities


CRC at a Glance

Total Build-up Area = 30000 Ft2

Buildings = 2

Annual Expenditure = @1% of TurnoverPersonnel Strength = 40 (Projected 2007) (Technical)

Educational Status

Number

Doctorates 8

Masters 24

Graduates 4

Total 36

Personnel Status(As on Aug 07)


• Gas Chromatograph• HPLC• TLC Scanner• UV Spectrophotometers • Moisture analyser (Infrared, Karl-Fischer)

• Viscometer • Mexameter• Skin Testing Equipment

(sebum level, moisture, pH, elasticity, TEWL)

• Hair Testing Equipment (tensile prop., combability, volume)

• Reflection Spectrophotometer

Major EquipmentsMajor Equipments(Analytical)


• Stability chambers• Rotary evaporator• Clean air workbench• Cold room• Ultra-low Temp Fridge (-70°C)• Washing Machine• Soxhlet extractor• Autoclave• Tray Driers

Major EquipmentsMajor Equipments(Infrastructural)

• Cell culture facility• Tergetometer• Triple Roll Mill• Contra-Rotary Mixer• Sigma Mixer• Simplex Plodder• Ribbon Mixer• Horizontal Mixer• Mini Soap Plodder• Rotary coating pan

• Generator Backup • Effluent Treatment Plant


Information / Knowledge ManagementInformation / Knowledge Management

• A fully networked facility with a central control (server) based on ‘thin client’ technology and a Document Management System

• Sample Tracking for all analytical operations including work flow

Information Systems


R&D ProgrammeR&D Programme


Extraction, isolation and characterisation of bioactive / functionalcompounds from natural sources

Synthesis, characterisation and new process development of ingredients for personal care products

Screening of natural products for microbiocidal activities with an emphasis on preservation of products and functional delivery (e.g. antidandruff)

Characterisation of major ingredients presently used in personal care products to optimise their performance

R&D Programme : Basic ResearchR&D Programme : Basic Research


Development of Formulations and Processes for Safe & EffectiveProducts with emphasis on functional delivery in the areas of -

Hair Care Products Skin Care Products Cleaning and Hygiene Products

Development of Spices, Vermicelli and Ready-to-Eat Products Such as Pickles, Nut based products (like peanuts, cashew), Snack foods and Traditional Sweets

Continuous optimisation and development of new formulationto keep the product cost under control

R&D Programme : Product DevelopmentR&D Programme : Product Development


Outline of R&D ActivitiesOutline of R&D Activities


• Focus on Benefits• Delivery of Promise (Claims substantiation)

• Superior Sensory Experience• Gentle / Safe

Product Development(Selection of Ingredients)

Product Development(Selection of Ingredients)


Appearance / ColourPerfume notepHActive DetergentViscosity in cpsFoam Height in cmsSalt ContentMicrobial Test

Typical Analysis

Product Development : ShampooProduct Development : Shampoo

SurfactantsFoam BoostersConditioning AdditivesViscosity ModifiersOpacifiersPearlizersPreservativespH modifiersPerfumeColour

Typical Ingredients


Sensory Evaluation for ProductsSensory Evaluation for Products


Product SupportProduct Support

• Identifying / Developing New Ingredients (biochemistry, microbiology & chemistry)

• Developing New Processes (Chemistry)

• Developing Specifications & Methods (Measurement)

• Demonstrating Efficacy (Biochemistry, Claim substantiation)

• Information / Knowledge Management (Library)

• Facilitating Activities (IT services, administration etc)

• Facing the Challenge of Contamination (Microbiology)


Claim SubstantiationClaim Substantiation

• Safety evaluation of Products e.g. Irritancy test• Developing study protocol• Identifying clinical investigator• Preparing documents like

Consent Form, Investigator Declaration Form, Product Brief, Assessment Form etc

• Recruitment of volunteers• Coordination and conduct of the study• Data collation, Analysis and Report submission


Graph 1 - Hair Fall Reduction in Volunteers After Use of Meera Oil and Parachute Coconut Oil

521

327

152

101

432427

501477

0

100

200

300

400

500

600

0 15 30 45 60 75 90

Days after oil use

No.

of h

airs

obt

aine

d

Meera Oil Users Parachute Coconut Oil Users

Graph 2 - Reduction in Hair Root Involved Hair Fall in Volunteers After Use of Meera Oil and Parachute Coconut Oil

103

2 0

284265

225

285309

0

50

100

150

200

250

300

350

0 15 30 45 60 75 90

Days after use

No.

of h

airs

with

root


Graph 3 - Increase in Anagen Hairs in Volunteers After Use of Meera Oil and Parachute Coconut Oil

79

69

37

22 192022

15

0

15

30

45

60

75

90

0 30 60 90Days after use

% o

f ana

gen

hairs


Chart 1 - Rating of Volunteers on Product Efficacy in Reducing Hair Fall

0

20

40

60

80

100

Poor Moderate GoodRating

% o

f vol

unte

ers


Note : No. of hairs obtained is the number of hair collected during combing in the clinic

Hair Oil - Study HighlightsHair Oil - Study Highlights(Claim Substantiation)


Evaluating a Skin Lightening CreamEvaluating a Skin Lightening Cream(Claim Substantiation)

15

10

31

0

2

4

6

8

10

12

14

16

Poor Moderate Good Excellent

Rating

Nu

mb

er o

f V

olu

nte

ers

Volunteer Perception of Efficacy

0

500

1000

1500

2000

2500

0 1 2 3 4

Weeks

Mex

amet

er R

ead

ing

Reduction in Average Mexameter Reading = 248% Reduction = 13.95 %

Assessing Reduction in Pigmentation


Biochemical AssaysBiochemical Assays

Mildness Assay (Shampoo) 1. Zein assay2. Collagen swelling3. pH rise

Skin Lightening Assay 1. Tyrosinase inhibition assay2. DOPA autooxidation inhibition assay

Anti-aging Assay Elastase inhibition asssay

Antiinflammatory Assay Soyabean lipooxygenase inhibition assay

Antioxidant Assay 1. DPPH radical scavenging assay2. Deoxyribose degradation assay3. Superoxide Dismutase assay


Skin Lightening 1. Melanin assay2. Tyrosinase inhibition assay

Oxidative stress Cytotoxicity Assay

Cytotoxicity / Proliferation MTT assay

Mildness Assay (Shampoo) 1. Cytotoxicity assay

2. RBC lysis assay

Cell Based AssaysCell Based Assays

Cell lines Available : Melanocyte (melanoma) B16-F10, B16-F1 & G361Fibroblast (normal) NIH / 3T3Keratinocyte (carcinoma) A431 General (cancer) HeLaPrimary cell lines Fibroblasts & Keratinocytes


Extraction, isolation and characterisation of bioactive / functionalCompounds from natural sources (skin lightening, antioxidants, antiaging etc.)

Screening of natural products for microbiocidal activities with an Emphasis on preservation of products and functional delivery (e.g. antidandruff)

ChemistryChemistry

Synthesis, characterisation and new process development of Ingredients for personal care products

Development of natural hair colours


Chemical ProcessChemical Process

RCOOH + HOC2H4NH2 RCO-NH C2H4OH + H2O176oC

Fatty acid MEA Monoethanol amide

RCOOCH2

RCOOCH

RCOOCH2

+ HOC2H4NH2 3 RCO-NH C2H4OH + Glycerin

Glyceride MEA Monoethanol amide ester (CNO)

RCOOCH3 + HOC2H4NH2 RCO-NH C2H4OH + MeOH

Fatty acid ester MEA Monoethanolamide

KOH

130-40 oC

130-35oC

KOH

1.

2.

3.

KOH

3

RCOOCH2

RCOOCH

RCOOCH2

+ N(CH2)3NH2 RCO-NH (CH2)3N + Glycerin

Glyceride DAPA Amidoamine ester

KOH

130-40 oC1a

1b RCO-NH (CH2)3N + ClCH2COONa RCO-NH (CH2)3N-CH2COO + NaCl

Amidoamine SMCA Cocoamidoproply betaine

2a R-COOH /RCOOMe

N(CH2)3NH2 RCO-NH (CH2)3N + H2O/ MeOHKOH

130-40 oC

Fatty acid/ DAPA AmidoamineMethyl ester

80-90C

3 3

3

+

Oleochemicals

1. Monoethanol amides2. Amidopropyl betaines

Secondary Surfactants

Also useful as Thickening Agent


MicrobiologyMicrobiology

• Screening natural products for antidandruff activity• Preservative Challenge Test

• Selection of suitable preservatives for products• Study of anti-microbial efficacy (MIC, synergy etc)

• Evaluation of competitive products

• Estimation of microbial load during development

Kill Kinetics of a Fungal Test Strain by an Antifungal

0

1

2

3

4

5

6

7

0 5 10 15 20 25 30Time (h)

Log

CFU

0.25x0.5x1x4xControl


MeasurementsMeasurements

Total No. Assays = >190

• Analysis of Own Products

• Analysis of Competitor Products

• Setting up Specifications

• Interfacing with Regulatory Authorities(BIS, Drug Controllers)

Major Responsibilities

SampleTracking

Confidential Document : Access to Approved Users OnlyConfidential Document : Access to Approved Users Only

confidential document : access to approved users only cavinkare research centre cavinkare pvt. ltd....

Documents

superior products

preservation of products

basic research slide

objectives of rd

safe effective products

performance rd programme

rd setup facilities

karthika hair wash powdermeera