conservation biology bisc 309 lec 2 - sfu.caconsgen1).pdf · conservation biology bisc 309 lec 2 ....
TRANSCRIPT
![Page 1: CONSERVATION BIOLOGY BISC 309 Lec 2 - SFU.caConsGen1).pdf · CONSERVATION BIOLOGY BISC 309 Lec 2 . REMINDER Course info Where is it? Go to biology homepage Click courses Click Conservation](https://reader033.vdocument.in/reader033/viewer/2022051804/5fefb6e8ce121136af3f7e0b/html5/thumbnails/1.jpg)
CONSERVATION BIOLOGY BISC 309 Lec 2
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REMINDER
Course info Where is it?
Go to biology homepage
Click courses
Click Conservation Biology
Course information
Tutorial material -readings
Lecture outlines
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Today Introduction to conservation genetics
Genetics (basics) – alleles, heterozygosity, genetic drift, inbreeding, quantitative traits Evolutionary Analysis
Conservation Genetics Ch 11. Principles of Conservation Biology 3e
Papers on Conservation Genetics Week 2 readings------ on course webpage Week 3 readings ----- coming
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IUCN (the World Conservation Union)
recognizes the need to conserve biodiversity at three levels
genetic diversity
species diversity
ecosystem diversity
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What causes extinction?
habitat loss introduced species overexploitation
So Why focus on genetics?
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Genetic variation may reduce extinction risk allow future adaptive evolutionary change
Genetic data can be a tool to help monitor populations
Why focus on genetics?
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“Wild species must have available a pool of genetic diversity if they are to survive environmental pressures exceeding the limits of developmental plasticity. If this is not the case extinction would appear inevitable”
OH Frankel 1983
“Gene pools are becoming diminished and fragmented into gene puddles” Thomas Foose 1983
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ROADMAP for next 2-3 lectures
Genetic Variation What is it?
How do we describe it?
What influences genetic variation? Ne, genetic drift, inbreeding
How important is inbreeding and the loss of genetic variation?
- fitness and extinction risk - ability to adapt
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Genetic material
1.! Coding (genes under selection) -! adaptive variation -! markers (allozymes, MHC, other genes)
2. Non-coding (not subject to selection) -! Neutral markers
variable number tandem repeat loci AFLP’s, RAPD’s, SNP’s
mini and microsatellites
Q. What are the limitations of microsatellite data?
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A species’ pool of genetic diversity exists at three fundamental levels
Loci - monomorphic no variation - polymorphic variable
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Genetic variation: individuals
A A A a
homozygous heterozygous
Heterozygosity - proportion of loci that are heterozygous
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Allelic diversity - number of alleles per loci
Average heterozygosity (H) – proportion of heterozygous loci in an average individual
H= 1 1
L ! total heterozygotes L total individuals
Genetic variation: within populations
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Genetic diversity in African lions
Allele Enzyme locus 1 2 3 Heterozygosity ADA 0.56 0.33 0.11 0.564 DIAB 0.61 0.39 0.476 ESI 0.88 0.12 0.211 GPI 0.85 0.15 0.255 GPT 0.92 0.11 0.196 MPI 0.92 0.08 0.147 20 other loci 1.00 0
Q. Calculate mean heterozygosity (H) and mean allelic diversity (A)
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Genetic variation: among populations
H2
H1
H3
D12 D13
D23
Ht=Hp + Dpt Total genetic variation =
average diversity within pop’ns + average divergence among pop’ns
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Red cockaded woodpecker
Overall mean heterozygosity = 0.078
Among population Dpt 14%
Within population Hp 86%
Q.!Are more vagile taxa (birds, insects) likely to have higher or lower Dpt
Q. How is variation likely to be partitioned in ibex (alpine) and white-tailed deer?
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FST = HT-HS /HT
= degree of genetic differentiation among pop’ns
Genetic variation: calculating F-statistics
Individual HI Subpopulation - random mating HS Total population - random mating HT
FST = 0, indicate pop’ns are same FST =1, indicates pop’ns have unique gene for each locus
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Mutation
Genetic drift
Inbreeding
All a function of EFFECTIVE population size
Ne
What influences genetic variation?
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Estimation of effective population size
Sex ratio Ne=(4 x Nm x Nf)/ (Nm + Nf)
Variation in RS Ne=k(Nk-1)/(Vk+k(k-1)) k, number surviving progeny Vk, variance in progeny number
Fluctuation in Population size 1/Ne= 1/t(1/N1+1/N2+…+1/Nt) Ne=t/!(1/Nei)
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Estimating effective population size. 1
If a harem has 1 male and 100 females, the effective size is
Ne =(4 x Nm x Nf)/ (Nm + Nf)
=
Unequal sex ratios reduce effective size
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Estimating effective population size. 2
When Vk/k >1 Ne is less than census size
Variance in LRS = 12.1 Mean family size, k=1.7
If captive population N=10
Ne=k(Nk-1)/(Vk+k(k-1))
Ne=
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Estimating effective population size. 3
Effective size is much closer to minimum than mean
Hunting reduced Northern elephant seal pop’n to 20-30 individuals before they recovered to over 100,000. Assume Ne1= 100,000
Ne2= 20 Ne3=100,000
Calc Ne Ne=t/(1/ Ne1 + 1/ Ne2 + 1/ Ne3) Ne=
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Effective population size
Ne can be estimated from demographic data on sex ratio, variance in family size, and pop’n fluctuations
Fluctuations in pop’n size have greatest impact on reducing Ne, followed by variance in population size
Estimates of Ne that incorporate all factors average only 11% of census sizes
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What influences genetic variation? Genetic drift:
random change in gene freq over time due to chance
If population crashes to Ne of 20 After 5 generations Predict 88% of heterozygosity remains
Change in heterozygosity
Ht=H0*(1-1/2Ne)t
Q. What assumptions are made in this eq’n?
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What influences genetic variation? Genetic drift:
Heterozygosity is lost more quickly from small pop’ns
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What influences genetic variation? Genetic drift:
random loss of alleles over time due to chance
Rare alleles are lost more quickly from small pop’ns
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Genetic drift
is the random change in gene frequency or random loss of alleles due to chance
the effects of chance are greater in small than large populations
population bottlenecks can lead to rapid loss of rare alleles and a reduction in heterozygosity
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What influences genetic variation Inbreeding
What is it?
Inbreeding is the mating of individuals related by ancestry
Inbreeding is unavoidable in small populations
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What influences genetic variation Inbreeding
How is it measured?
The inbreeding coefficient of an individual (F)
- refers to how closely related its parents are
- is the probability that it carries alleles at a locus that are identical by descent
Unrelated F=0 Brother-sister F=0.25 First cousins F=0.0625 Self-fertilization F=0.5
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What influences genetic variation Inbreeding
How is it calculated?
pedigrees F=!(1/2)n (1+Fca)
Only 1 pathway link the two parents
n=7, FDBACEG
If A not inbred, Fca=0
Fx= (1/2)n = 1/128
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What influences genetic variation Inbreeding
How is it calculated?
pedigrees F=!(1/2)n (1+Fca)
Q.!Calculate Fx for this male dorca’s gazelle Hint - there are 5 paths and FE=1/4
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What influences genetic variation Inbreeding
Inbreeding accumulates over time
Inbreeding accumulates more rapidly in small pop’ns, "F= 1/2Ne
F
generation
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What influences genetic variation Inbreeding
How else is it calculated?
heterozygosity data
Ht/H0 = (1-1/2Ne)t = 1-F
Fe = 1-(Ht/H0)
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Estimating population inbreeding coefficients
Fe = 1-(Ht/H0) Fe =
Isle Royale gray wolves Established in 1949 n = low 1980 n=50, 1990 n=14 Allozymes 25 loci
heterozygosity = 3.9% Mainland pop’n
heterozygosity = 8.7%
Q. Calculate inbreeding coefficient for the island
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What influences genetic variation Inbreeding
What does inbreeding do?
Reduces the frequency of heterozygotes Exposes deleterious recessive mutations
May therefore reduce fitness Does NOT change allele frequencies
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Inbreeding
Is mating of individuals related by ancestry
Accumulates over time and does so more rapidly in small populations
the increment in inbreeding per generation is equal
to the loss of heterozygosity
Exposes any deleterious recessive mutations
May reduce fitness
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Frankham 1996 summarized available data
Species with more diversity have larger pop’ns and occupy more area Does this mean greater diversity is beneficial?
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TODAY You should be able to:
Calculate Ne,and F Understand how:
Ne affects drift and inbreeding Drift and inbreeding influence genetic
diversity
NEXT How do inbreeding and the loss of genetic variation influence
- fitness and extinction risk - ability to adapt
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