consumer products study disinfectants, mouthwash antibacterial hand soap, antibiotics, and spices by...
TRANSCRIPT
Consumer Products Study
Disinfectants, Mouthwash Antibacterial Hand Soap, Antibiotics, and Spices
By THE WESTON SCHOLARS!!!!! (and gifted and talented students.)
MATERIALS Tape
Wax pencil Petri dish
Cotton swabs Forceps
Nutrient broth Sterile disk
Inoculating wire loop Sterile water
Bunsen burner Nutrient agar plate
All consumer products
Procedure
1. Suspend 23g of nutrient agar powder in 1L purified H2O.
2. Mix and heat solution until powder is dissolved and solution becomes an translucent yellow.
3. Autoclave at 121ºC for 15 minutes.
4. Fill as many petri dishes as possible (half full) with nutrient agar and let them solidify.
Preparing Nutrient Agar Plates
Procedure (cont’d)
5. Dip cotton swab in sterile H2O and take samples from mouth, skin, and environment.
6. Rub the swab on nutrient agar plate.
7. Cover nutrient agar plates and label them.
8. Invert nutrient agar plates and place them into the incubator at 37ºC.
Procedure (cont’d)9. For discontinuous streaking, sterilize wire
loop by flaming until the wire turns bright orange.
10.Cool loop by lightly touching the wire to the nutrient agar.
11.Using the wire loop, take bacteria from the nutrient agar plate and streak 4 times on a new agar plate and flame the loop in between.
12.Cover, label, and tape plates shut. 13.Place, inverted, in the incubator at 37°C.
Procedure (cont’d)
14. For the continuous streaking, use the same procedure as the discontinuous streaking, except you just streak back and forth until you cover the whole nutrient agar plate.
Procedure (cont’d)Re-suspend bacteria
1. Pour 10ml of nutrient broth into the continuously streaked plates.
2. Swab bacteria until all bacteria is suspended in liquid3. Swab bacteria into new plates.4. Dip sterile disk in disinfectants, antibiotics, spices,
mouthwash, or antibacterial hand soap and place in center of plates. Tap gently to secure.
5. Label plates and place them inverted in the incubator. 6. Let plates sit in the incubator over night.7. Measure the zone of inhibition (in millimeters) and make
your conclusions.
Disinfectants• Ajax• Algacide• America’s Choice Pine Cleaner• Ammonia• 10% Bleach• Clorox• Fantastik-Clear• Fantastik-Purple• Scrubbin’ Bubbles• Soft Scrub• Spic and Span• Tilex• Touch of Glass• Wavicide• White Vinegar• Windex• Xtra Pine• 409
ResultsDisinfectants Zone of Inhibition
Ajax 25mm
Algacide 19mm
America’s Choice 9mm
Clear Ammonia 0mm
Clorox 30mm
Clear Fantastik 11mm
Purple Fantastik 14mm
Scrubbin’ Bubbles 10mm
Soft Scrub 9mm
Spic and Span 12mm
Tilex 9mm
Touch of Glass 1mm
Wavicide 5mm
White Vinegar 15mm
Windex 1mm
Xtra Pine 10mm
409 23mm
10% Bleach 0mm
Results
Disinfectants Zones of I nhibition
25
19
9
0
30
11
14
109
12
9
1
5
15
1
10
23
00
5
10
15
20
25
30
35
Disinfectants
Zo
ne o
f In
hib
itio
n (
mm
)
Ajax
Algacide
Americas Choice
Clear Ammonia
Clorox
Fantastik-Clear
Fantastik-Purple
Scrubbin Bubbles
Soft Scrub
Spic and Span
Tilex
Touch of Glass
Wavicide
White Vinegar
Windex
Xtra Pine
Four o' Nine
Ten Percent Bleach
Mouthwashes
• Biotene• Cepacol• CVS Brand• Diluted Hydrogen Peroxide• Listerine• Listerine Tartar Control• Plax• Rite Aid Brand• Salt Water • Scope• ShopRite Brand• Tom’s Natural Mouthwash
Results
Mouthwashes Zone of InhibitionBiotene 2mm
Cepacol 12mm
CVS Brand 3mm
Hydrogen Peroxide 18mm
Listerine 2mm
Listerine Tartar Control 2mm
Plax 10mm
Rite Aid Brand 3mm
Salt Water 0mm
Scope 3mm
ShopRite Brand 3mm
Tom’s Natural Mouthwash 0mm
Results
Mouthwash's Zones of Inhibition
2
12
3
18
2 2
10
3
0
3 3
00
2
4
6
8
10
12
14
16
18
20
1
Mouthwashes
Zon
e o
f In
hib
tion
(m
m)
BIOTENE
CEPACOL
CVS
DILUTED HYDROGENPEROXIDELISTERINE
LISTERINE TARTARCONTROLPLAX
RITE AID
SALT WATER
SCOPE
SHOPRITE
TOMS NATURALMOUTHWASH
Hand Soaps
• Bath & Body Works
• Clean n Smooth
• Ajax
• Palmolive
• Soft Soap
• April Body & Shower
Results
Type of Soap Zone of InhibitionClean & Smooth 4mm
Bodyworks 1mm
Softsoap 17mm
Palmolive 15mm
April Bath & Shower 3mm
AJAX 18mm
Results
Soaps
-1.002.003.004.005.006.007.008.009.00
10.0011.0012.0013.0014.0015.0016.0017.0018.0019.00
Name of Soap
Zo
ne o
f In
hib
itio
n
Antibiotics on the skin bacteria
• Tetracycline • Rifampin • Chloramphenicol • Kanamycin • Streptomycin• Triple Sulfonides• Polymyxin B• Erythomycin• Cephalothin
Results
Name of Antibiotics Zone of Inhibition Sig. of Zone of Inhibition
Tetracycline 21mm Sensitive
Cephalothin 25mm Sensitive
Rifampin 28mm Sensitive
Chloramphenicol 23mm Sensitive
Kanamycin 18mm Sensitive
Streptomycin 12mm Intermediate
Triple Sulfonides 0mm Resistant
Polymyxin B 10mm Intermediate
Erythomycin 15mm Resistant
Spices• Cinnamon• Paprika• Chili Powder• Onion Powder• Cream of Tartar• Coriander• Mustard Powder• All Spice• Alum• Cumin• Nutmeg• Cajun Seasoning• Curry• Ground Ginger• Garam Marsala• Garlic
ResultsCinnamon 5mm
Paprika 0mm
Chili Powder 3mm
Onion Powder 2mm
Cream of Tartar 9mm
Coriander 0mm
Mustard Powder 4mm
Allspice 6mm
Alum 10mm
Cumin 4mm
Nutmeg 4mm
Cajun Seasoning 2mm
Curry 3mm
Ground Ginger 2mm
Garam Masala 4mm
Garlic 10mm
Spices Zone of Inhibition
ResultsSpices as Disinfectants
5
0
3
2
9
0
4
6
10
4 4
2
3
2
4
10
0
2
4
6
8
10
12
Spices
Zo
ne
of
Inh
ibit
ion
(M
M)
Cinnamon
Paprika
Chili Powder
Onion Powder
Cream of Tartar
Coriander
Mustard Powder
All Spice
Alum
Cumin
Nutmeg
Cajun Seasoning
Curry
Ground Ginger
Garam Marsala
Garlic