control options rice_bacterial_panicle_blight
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C t l O ti f RiC t l O ti f RiControl Options for Rice Control Options for Rice Bacterial Panicle BlightBacterial Panicle BlightBacterial Panicle BlightBacterial Panicle BlightGroth D EGroth D E 11 Rush M CRush M C 22 Shahjahan A K MShahjahan A K M 22Groth, D.E.Groth, D.E. , Rush, M.C., Rush, M.C. , Shahjahan, A.K.M., Shahjahan, A.K.M. , ,
Sha, X.Sha, X.11, and Ham, J., and Ham, J. 22
LSU AgCenter, LSU AgCenter, 11Rice Research Station, Rayne, LA and Rice Research Station, Rayne, LA and 22Department of Plant Pathology and Crop Physiology, Baton Department of Plant Pathology and Crop Physiology, Baton
Ro ge LA USARo ge LA USARouge, LA USARouge, LA USA
Major Rice Diseases Major Rice Diseases
Bacterial Panicle BlightBacterial Panicle Blight
Areas of researchAreas of researchAreas of researchAreas of research
EtiologyEtiologyEtiologyEtiologyEpidemiologyEpidemiologyC lt l tC lt l tCultural managementCultural managementFoliar pesticidesFoliar pesticidesSeed treatmentsSeed treatmentsDisease resistanceDisease resistanceDisease resistanceDisease resistance
EtiologyEtiologyEtiologyEtiology
Isolation by plant inoculationIsolation by plant inoculationIsolation by plant inoculationIsolation by plant inoculationBacterial species as determined by BioLogBacterial species as determined by BioLogBurkholderia glumaeBurkholderia glumae most prevalentmost prevalentBurkholderia glumaeBurkholderia glumae most prevalentmost prevalentOthers identifiedOthers identified
–– B gladioliB gladioliB. gladioliB. gladioli–– B. cepaceaB. cepacea–– B. pyrrpciniaB. pyrrpciniapy ppy pDevelopment of a realDevelopment of a real--time PCR to identify time PCR to identify B. B. glumae glumae andand B. gladioliB. gladioli in green tissues in green tissues
Isolation and identificationIsolation and identificationIsolation and identificationIsolation and identification
EpidemiologyEpidemiologyB. gladioli found surviving in soil but not B. B. gladioli found surviving in soil but not B. glumaeglumaeglumaeglumaeSpread of epiphytic populations limited Spread of epiphytic populations limited during growing seasonduring growing seasonduring growing seasonduring growing season38 and 4038 and 40°°C for optimum for bacteriaC for optimum for bacteria
Cultural managementCultural managementCultural managementCultural management
Nitrogen managementNitrogen managementNitrogen managementNitrogen management–– Higher N rate more diseaseHigher N rate more disease
Planting datePlanting date–– Late planted rice more diseaseLate planted rice more disease
Exposed to higher temperaturesExposed to higher temperatures
Foliar ApplicationsFoliar ApplicationsFoliar ApplicationsFoliar Applications
AntibioticsAntibioticsAntibioticsAntibioticsCoppersCoppersO li i A idO li i A idOxolinic AcidOxolinic AcidMicro nutrient mixturesMicro nutrient mixturesGrowth regulatorsGrowth regulators
Tested Products Tested Products InIn--vitrovitro
Foliar TrialsFoliar TrialsFoliar TrialsFoliar Trials
Applied between boot and headingApplied between boot and headingApplied between boot and headingApplied between boot and headingVarious rates ex. Starner 0.35Various rates ex. Starner 0.35--0.50 lb 0.50 lb aiai/A/AI l t d d i l t d t i lI l t d d i l t d t i lInoculated and uninoculated trialsInoculated and uninoculated trialsCoppers tend to be toxicCoppers tend to be toxicAntibiotics were not very effectiveAntibiotics were not very effectiveOxolinic acid (Starner 20WP) bestOxolinic acid (Starner 20WP) bestOxolinic acid (Starner 20WP) bestOxolinic acid (Starner 20WP) best
Difficulties with foliar applicationsDifficulties with foliar applicationsDifficulties with foliar applicationsDifficulties with foliar applications
No prediction or scouting methodsNo prediction or scouting methodsNo prediction or scouting methodsNo prediction or scouting methodsErratic occurrenceErratic occurrenceR l t blR l t blRegulatory problemsRegulatory problemsCost of preventative spraysCost of preventative spraysToxicity of coppersToxicity of coppersIncreased yields and milling erraticIncreased yields and milling erraticIncreased yields and milling erraticIncreased yields and milling erratic
N h i l t l i th f tN h i l t l i th f tNo chemical control in the near futureNo chemical control in the near future
Scouting and Determining NeedScouting and Determining Need
Damage is most severe during periods of Damage is most severe during periods of unusually hot weather or unusually hot nights. No unusually hot weather or unusually hot nights. No y y gy y gscouting methods are available and no chemical scouting methods are available and no chemical control agents are labeled to control bacterial control agents are labeled to control bacterial panicle blightpanicle blight. .
Seed treatmentsSeed treatmentsSeed treatmentsSeed treatments
Materials testedMaterials testedMaterials testedMaterials tested–– Coppers fungicidesCoppers fungicides
AntibioticsAntibiotics–– AntibioticsAntibiotics–– Oxolinic acidOxolinic acid
D l t f ELISA d PCR t t fD l t f ELISA d PCR t t fDevelopment of ELISA and PCR tests for Development of ELISA and PCR tests for identifying infected seedidentifying infected seed--lots lots Seed treatments erraticSeed treatments erratic
Disease resistanceDisease resistanceDisease resistanceDisease resistance
Screening methodsScreening methodsScreening methodsScreening methods–– Inoculation at boot split and headingInoculation at boot split and heading
Bacterial concentration critical ~1x10Bacterial concentration critical ~1x1088–– Bacterial concentration critical ~1x10Bacterial concentration critical ~1x1088
CFU/mlCFU/ml
–– Too high all susceptibleToo high all susceptible
–– Too low all are resistantToo low all are resistant
–– Isolate must produce toxinIsolate must produce toxin
Inoculation timingInoculation timing -- 2 times2 timesInoculation timing Inoculation timing 2 times2 times
Rating scale (0Rating scale (0--9)9)0 no damage (Immune)0 no damage (Immune)3 203 20--30% damage (moderately resistance)30% damage (moderately resistance)3 203 20 30% damage (moderately resistance)30% damage (moderately resistance)6 506 50--60% damage (susceptible)60% damage (susceptible)9 100% t ilit ( tibl )9 100% t ilit ( tibl )9 100% sterility (very susceptible)9 100% sterility (very susceptible)
2 vs 8
Disease resistance cont.Disease resistance cont.Host resistanceHost resistance–– High levels of resistance found (1High levels of resistance found (1--3 ratings)3 ratings)
Nipponbare, LMNipponbare, LM--1, Jupiter, TeQing, AB647, others1, Jupiter, TeQing, AB647, others–– Crosses made and populations being evaluatedCrosses made and populations being evaluated–– Evaluating 6000Evaluating 6000--8000 inoculated rows/year8000 inoculated rows/year–– Evaluate whenever natural disease developsEvaluate whenever natural disease developspp–– Screen FScreen F33 to Fto F88
Panicle Blight ReactionsPanicle Blight ReactionsPanicle Blight ReactionsPanicle Blight Reactions
Very Susceptible Susceptible
Moderately Susceptible
Moderately Resistant
CL131 CL161 Catahoula JupiterBengal Cheniere Hybridsg yTrenasse CocodrieNeptune CL151NeptuneWells
CL151CL171
10 BPB resistant lines, including source 10 BPB resistant lines, including source varieties, germplasm, and breeding lines, varieties, germplasm, and breeding lines,
are available by requestare available by request
LM-1NPB/CCDR
NipponbareNipponbareLM‐1/CCDR
Jupiter LR2065/CCDRJup te /
LM‐1/CCDR CCDR/LR2065NBP(MCR00 2190 C93 137 LR2065/CCDRNBP(MCR00‐2190 C93‐137 KATY/CPRS/JA85)
LR2065/CCDR
SummarySummaryCultural management ineffectiveCultural management ineffectiveChemical control unlikelyChemical control unlikelyChemical control unlikelyChemical control unlikelyDisease resistance main area of effortDisease resistance main area of effortSome progress towards developing Some progress towards developing diseases resistance including four sourcesdiseases resistance including four sourcesRequest seed fromRequest seed from:: Don GrothDon Groth
Rice Research StationRice Research StationRice Research StationRice Research Station1373 Caffey Road1373 Caffey RoadRayne LA 70578 USARayne LA 70578 USARayne, LA 70578 USARayne, LA 70578 USA
[email protected]@agcenter.lsu.edu