convergent evolution of squid photophores and transcriptomes
DESCRIPTION
Slides describing research published in PNAS, and BMC Evolutionary Biology. We found that light producing photophores evolved separately in two distantly related squid. Surprisingly, overall gene expression is amazingly similar.TRANSCRIPT
![Page 1: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/1.jpg)
Cephalopod’s Tale(PhD thesis of Sabrina Pankey)
What are bacteriogenic photophores, what do they do?
They evolved more than once in cephalopods
Transcriptomes of convergent photophores are unexpectedly,predictably similar
![Page 2: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/2.jpg)
What If…We Could “Replay the Tape””
![Page 3: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/3.jpg)
Does molecular convergence follow phenotypic convergence?
![Page 4: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/4.jpg)
What are bacteriogenic photophores, what do they do?
![Page 5: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/5.jpg)
Photophores of Squid House a Vibrio symbiosis
![Page 6: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/6.jpg)
Physiological(Response to light)Morphological
Gq
R-opsin
TRP
PLC
Phototransduction Genes
Tong,Rosas,Oakley,Mitchell,Colley,McFall-Ngai. PNAS (2009)
Transcription factors Pax6, Dac, Eya, Six
Peyer, Pankey, Oakley,McFall-Ngai. Mech Dev (2014)
![Page 7: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/7.jpg)
Bacteriogenic photophores evolved more than once.
![Page 8: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/8.jpg)
A phylogenetic hypothesis for convergent origins
![Page 9: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/9.jpg)
A phylogenetic hypothesis for convergent origins
![Page 10: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/10.jpg)
Distantly related squid harboring bioluminescent symbionts
![Page 11: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/11.jpg)
Distantly related squid harboring bioluminescent symbionts
![Page 12: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/12.jpg)
Distantly related squid harboring bioluminescent symbionts
![Page 13: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/13.jpg)
Distantly related squid harboring bioluminescent symbionts
![Page 14: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/14.jpg)
Overall gene expression in convergent photophores is unexpectedly similar.
![Page 15: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/15.jpg)
How does overall gene expression in convergent traits compare to expression in
homologous traits?
![Page 16: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/16.jpg)
Most variation in gene expression is species differences
![Page 17: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/17.jpg)
Photophores cluster together based on gene expression
![Page 18: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/18.jpg)
Convergent Transcriptomes as similar as Homologous Transcriptomes
Pankey et al PNAS (2014)
![Page 19: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/19.jpg)
Conclusions
![Page 20: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/20.jpg)
A ‘Replay of the Tape’ yielded remarkably similar outcomes
![Page 21: Convergent evolution of squid photophores and transcriptomes](https://reader033.vdocument.in/reader033/viewer/2022042614/557d79e9d8b42a75548b4b19/html5/thumbnails/21.jpg)
Does molecular convergence follow phenotypic convergence?