corning innovation showcase€¦ · paula flaherty technology manager deepa saxena, ph.d. senior...

Paula FlahertyTechnology ManagerDeepa Saxena, Ph.D.Senior Development ScientistISSCR 2015 June 26, 2015

ISS

CR

2015Corning Innovation ShowcaseStem Cell Culture, Differentiation, and Scale-Up

Novel Technologies Enabling Research and Cell Processing Applications

2© 2015 Corning Incorporated

Today’s Topics

• Introduction: Corning® Life Sciences• Corning cell culture surfaces technologies• Industry trend: Animal-free • Corning PureCoat™ ECM mimetic cultureware

Collagen I peptideFibronectin peptide

• Scale up for cell manufacturing: Examples

• Corning hPSC culture substrates for expansion and differentiation

ISS

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2015


Corning Incorporated HighlightsFounded:1851

Headquarters:Corning, New York

Employees:~35,000 worldwide

2014 Sales:$10B

R&D Investment:~10% of sales

Fortune 500 Rank (2015): 297

• Corning is one of the world’s leading innovators in materials science. For more than160 years, Corning has applied its unparalleled expertise in specialty glass, ceramics, and optical physics to develop products that have created new industries and transformed people’s lives.

• Corning succeeds through sustained investment in R&D, a unique combination of material and process innovation, and close collaboration with customers to solve tough technology challenges.


Corning: Leader in Cell Culture Surface InnovationA Century of Surface Technology

1915 Heat-resistant Pyrex® glass

1907Glass Surfaces used to culture

mammalian cells

1910 1920 1930 1940 1950 1960 1970 1980 1990 20102000

2010Synthemax®

Synthetic VN peptide surface

2012

1959 TC treatment and

gamma irradiation

TC-treated Polystyrene

1985Matrigel® matrix, defined ECMs

In vivo-like cell growth and differentiation

1987BioCoat™ surface

Convenient and consistent pre-coated cultureware

2012PureCoat™ ECM

Mimetic Fibronectin and Collagen I

Synthetic peptide surfaces

1990sPrimaria™

and CellBIND®

Charged-based modification of plastic surfaces

2000sPureCoat surfacesAmine and CarboxylUltra-Low AttachmentHydrogel-coated low bind surface

1953Development of

plastic 100 mm petri dish for U.S. Army

Glass + Plastic + Biological + Synthetic

2015

2014Corning

rLaminin-521Animal-free pluripotent

stem cell surface


Cell Manufacturers are Rapidly Progressing from Scientific Knowledge to Process Engineering

“Critical Product Quality and Process Controls” has been identified as a major technology need for cell manufacturers*

Cell manufacturing is moving towards less animal products in their processes Reducing the risk of adventitious agent contamination Enabling control of cell manufacturing process

• Reducing variability of raw materials• Reducing testing required lot-to-lot• Progressing from fundamental science to engineering

Enabling optimization of process Supporting predictive productivityFacilitating downstream purification

*Cell Manufacturing Consortium, US National Institute of Standards and Technology (NIST), Advanced Manufacturing Technology, Identifying Industry Needs, 05.08.15

Science and TechnologyDevelop fundamental understanding

of product, process, and material

Engineering/ManufacturingDevelop and control advanced

product and process technology


Complete and Customizable Cell Culture Environments

• Corning® Matrigel® matrix• Purified biological, xeno-free, and

synthetic ECMs• ECM mimetic cultureware

• cGMP media manufacturing• Serum• Serum-free media supplements and

growth factorsCorning CellSTACK®

Multi-layer flasks

Coated microcarriers

Customizable solutions for all stages of cell and tissue culture research


Advanced Surfaces and Extracellular Matrices

Vialed Extracellular Matrices (ECMs)Corning® Matrigel® matrix Defined ECMs

Animal-derivedXeno-free

Recombinant ECMsrLaminin-521 (Human)

Synthetic ECMs SynthemaxCorning PuraMatrix™

Custom ECM formulations

Synthetic ECM Mimetic Pre-coated Cultureware

Corning PureCoat™Fibronectin peptideCollagen I peptiderLaminin-521 coming soon

• Synthemax (custom)Vitronectin peptide

Biological ECM Pre-coated CulturewareCorning BioCoat™

• Plates• Inserts• Flasks• Coverslips/slides• Dishes

•Custom coatings

Enhanced Tissue Culture-treatedCorning CellBIND® surface

(-) chargeCorning PureCoat

Amine (+) chargeCarboxyl (-) charge

Corning Primaria™(+) and (-) mixed charge

Ultra-Low Attachment Hydrogel-coated

Functional Attachment ChargeFunctional Attachment Functional Attachment

General cell culture, HTS, specialized applications HTS, general cell culture Specialized applications:

cell culture enablement General cell culture, HTS, specialized applications

Corning® PureCoat™ ECM Mimetic Cultureware

Synthetic peptide covalently attached on a surface

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Corning® PureCoat™ ECM Mimetic Cultureware

• Animal-free, synthetic peptide (Collagen I peptide or Fibronectin peptide) covalently attached on a surface

• Support attachment and expansion of cell types that require Collagen I or Fibronectin ECM coating

• Pre-coated, ready to use• Stable at room temperature for 18 months• Manufactured in a GMP compliant facility• US Class I medical device • Sterile by aseptic processing• Compatible with a wide range of dissociation reagents

and serum-free, xeno-free, and animal-free media• Available in 24-well, 6-well, T-75, T-175, 3-Layer or

5-Layer Multi-Flask format, and custom Corning CellSTACK®


Corning® PureCoat™ ECM Mimetic CulturewareManufacturing and Quality

• Manufactured under cGMP per the requirements of FDA 21 CFR 820

• Manufacturing facility ISO 9001, ISO 13485, and ISO 14001 certified

• Animal-free manufacturing process

• Quality control confirms the following:

NonpyrogenicNontoxicFunctional activity Sterile

• Regulatory documentation available

ISO CertificatesCertificate of Compliance Certificate of Analysis

Lot-specific QC results

End-user

No animal components in final product

No animal components used in production of raw materials


Synthetic and Defined Cultureware Suitable for Expansion of a Variety of Cell Types

Corning® PureCoat™

ECM MimeticExamples of Cell Types Expanded in Defined Cell Culture Environments

Fibronectin Peptide Human BM-derived mesenchymal stem cells

Human endothelial progenitor cells

Human adipose-derived stem cells

CHO cells

Vero cells

Collagen I Peptide Human keratinocytes

Human corneal cells

Human adipose-derived stem cells

Human endothelial progenitor cells

Human cord blood-derived mesenchymal stem cellsVero cells

Corning® PureCoat™ ECM Mimetic CulturewareCollagen I peptideSynthetic surface for cell types that require Collagen I coating IS

SC

R 2015


Corning® PureCoat™ Collagen I Peptide Supported Human Keratinocyte Culture and Functionality

• Human neonatal keratinocytes

• Culture in animal-free medium EpiLife® with S7 supplements

• Control: Rat tail collagen, and ECM matrix recommended with the medium

Collagen I Mimetic Coating MatrixRat Tail Collagen I

Comparable growth on 3 surfaces

Cumulative population doubling

02468

101214

1 2 3 4

Passage number

Popu

latio

n do

ublin

g

Collagen I mimeticRat tail collagen ICoating matrix

Time 0 2 hr

3 hr 4 hr

Division-arrested cells migrated to heal scratch wound

Comparable cell morphology

Corning® PureCoat™ ECM Mimetic CulturewareFibronectin peptide Synthetic surface for cell types that require Fibronectin coating IS

SC

R 2015


Xeno-free Human Mesenchymal Stem Cell Culture on Fibronectin Mimetic Surface

• Human bone marrow-derived MSCs in MesenCult™-XF medium

• Control: Human-origin coating matrix

Fibronectin Mimetic Human-origin matrix

AdipogenicOsteogenic

Comparable morphology and growth on Fibronectin mimetic and human-origin matrix.

Cells maintained multipotency after expansion on Fibronectin mimetic.

Cells expressed ISCT established marker profile.

Successfully tested with additional xeno-free media.


Human Endothelial Colony Forming Cell (ECFCs®) Culture on ECM Mimetic Collagen I and Fibronectin Peptide

• Culture for 4 passages in EGM™-2 BulletKit™

containing 2% FBS (Lonza)DiI-acetylated-LDL uptakeDifferentiation on Corning® BioCoat™

angiogenesis system: endothelial cell tube formation

• Controls: Corning rat tail Collagen I, human Fibronectin

Cumulative population doubling of ECFCs

0

2

4

6

8

10

12

2 3 4Passage number

Pop

ulat

ion

doub

ling

Collagen I Mimetic Rat tail collagen IFibronectin Mimetic Human fibronectin

ECFCs demonstrated typical morphology, functionality and differentiation after expansion on Corning PureCoat™ ECM mimetic cultureware.

Collagen I Mimetic Fibronectin Mimetic

Mor

phol

ogy

DiI-

Ac-L

DL

upta

ke

Tube

form

atio

n

ECM mimetic Fibronectin and Collagen I both supported ECFC® expansion under low-serum conditions.

Corning® stemgro® hMSC Culture Environment

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Corning® stemgro® hMSC Culture Environment• Serum-free, xeno-free, defined medium• In combination with Corning CellBIND® surface

provides complete cell culture environment for hMSCs

• Enables efficient expansion of hMSCsCorning Cat . No.40-410-KIT

stemgro/CellBIND

10% FBS/TC-treated Competitor/CellBIND


Surface Markers, Karyotype and Differentiation After 7 Passages in Corning® stemgro® hMSC Medium

Medium/Surface Passage # CD73 CD90 CD105 CD14 CD45

Input Cells p0 95.2 95.0 96.8 0.0 0.0

stemgro/CellBIND® p5 98.5 99.2 96.7 0.0 0.0

Competitor/CellBIND p5 88.4 93.0 95.1 0.0 0.0

Adipogenic- Oil Red O Osteogenic Alizarin Red Chondrogenic Alcian Blue

Scale-up and Manufacturing

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Cost-effective Scale-up in Corning®

PureCoat™ ECM Mimetic Multi-Flask• Easy and cost effective scale-up with

consistent performance

• Uniform cell growth between the layers results in equivalent cell yield/cm2 in T-175, 3-Layer and 5-Layer ECM mimetic Multi-Flasks

Scale-up

0

5000

10000

15000

20000

25000

30000

35000

T-175 3-Layer 5-Layer

Cel

l yie

ld /c

m s

q.

MSCsKeratinocytes

Cost/ sq. cm.

Cost effective

Equivalent cell yield

Uniform cell growth


Corning® PureCoat™ ECM Fibronectin Mimetic Closed System Corning CellSTACKs® Have Been Qualified to Manufacture MSC Treatment for Graft-versus-Host Disease

Typical MSC morphology was observed for the MSCs cultured on Fibronectin mimetic surface.

400 µm400 µm

020406080

100120

CS2L T175 T75

% Viability, 9 Days in Culture

0.00E+00

2.00E+07

4.00E+07

6.00E+07

8.00E+07

1.00E+08

T-75 T-175 ML5 CS2 CS10

Cell Yield/Vessel Type

Viability of MSCs cultured on Fibronectin mimetic Corning CellSTACK 2-layer, T-175, and T-75 flasks were comparable.

Cell yields from T-75, T-175, 5-Layer Multi-Flask (n=3), Corning CellSTACK (n=3), and 10-Layer demonstrated scalable yields.


MSCs Maintained Characteristic Immunophenotype and Multi-lineage Differentiation Capability

MSCs were positive for CD13, CD29, CD44, CD73, CD90, CD105, and negative for CD45, CD80, and HLA-DR.

MSCs were differentiated into adipogenic, osteogenic, and chondrogenic lineages.

Adipogenic (Oil red O) Chondrogenic (Alcian Blue)Osteogenic (von kossa)

Poster Presentation IIT-10009 6/25/15


Xeno-free Culture of Human Adipose Stem Cells (hASC) in Corning®

stemgro® hMSC Medium and Corning CellBIND® Surface Cells exhibited a spindle-like morphology, and at a confluence higher than 75%, cell colonies formed a typical “river-like” pattern of growth.

Average doubling time (h) for two sub-culturing steps of expanded cells, for hASC061 and hASC064 was similar, 51 and 54h, respectively. For hASC057 a shorter doubling time was observed, i.e., an average time of 32h. Viability of expanded cells varied from 93% to 99%.


hASCs Maintained Characteristic Immunophenotype and Multi-lineage Differentiation Capability

Poster Presentation IIT-10010 6/25/15

Concomitant expression of CD73, CD90 and CD105 in the absence of CD34 range between 90.0 to 97.6%. Cells expressed low hematopoietic and endothelial markers, i.e., expression of 1.3% for CD31, 2.5% for CD34 and 0.5%, for CD45.

Successful differentiation into the three mesenchymal lineages


Summary

Corning® PureCoat™ ECM mimetic cultureware: • Synthetic, defined, and animal-free• Compatible with multiple media • Support xeno-free expansion of multiple cell types while maintaining functionality• Provide a ready to use alternative to ECM protein coating where animal-free and

defined conditions are desirable• Demonstrated to support cell manufacturing scale

Corning stemgro® hMSC culture environment:• Serum-free, xeno-free, defined medium• In combination with Corning CellBIND® surface provides complete cell culture

environment for hMSCs• Enables efficient expansion of hMSCs• Has demonstrated expansion of hASC derived from xeno-free cGMP cryopreserved

human stromal vascular fraction (hSVF)

Stem Cell Culture, Differentiation, and Scale-up – Novel Technologies Enabling Research and Cell Processing Applications

Deepa Saxena, Ph.D.

June 26, 2015ISSCR Annual Meeting, Stockholm

ISS

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2015


Outline

• Feeder based human pluripotent stem cell (hPSC) culture and limitations

• Corning® hPSC culture substrates Corning Matrigel® hESC-qualified matrix Corning Synthemax®-SCCorning rLaminin-521 (Human)

• Corning PureCoat™ rLaminin-521 cultureware coming soon!

• Corning matrices for stem cell differentiation

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hESC on Matrigel matrix

http://stemcells.nih.gov/StaticResources/info/scireport/images/figurec1.jpg

HPSC culture on mouse embryonic fibroblasts (MEF) feeder layer

• Limitations• Labor intensive isolation and maintenance• Variation of different MEF lots• Undefined media composition• Low transfection efficiency, some “quenching”

from the feeder layer• Potential contamination with animal pathogens • Difficult to isolate pure DNA, RNA due to cross-

contamination from feeder layers

hESC on MEF feeder cells


Key challenges of hPSC culture

• Lack of complete culture environment with a standardized protocol

• Spontaneous differentiation leading to impurity or heterogeneity of propagated cells

• Scaling up cultures – difficulty due to clump passaging

• Time required to evaluate a new culture condition (undifferentiated expansion for multiple passages, differentiation, karyotype analysis)

Corning® Matrigel® hESC-qualified Matrix

Animal-origin matrix containing extracellular matrix (ECM) proteins and growth factors IS

SC

R 2015


Corning® Matrigel® hESC-qualified matrix

• Gold standard for hPSC culture– Human embryonic stem cells. Xu, et al. Nature

Biotechnology (2001).– Induced pluripotent stem cells. Junying, et al.

Science (2007); Takahashi, et al. Cell (2007).

• Compatible with multiple hPSC culture media – mTeSR™1

– TeSR™ 2

– E8

– NutriStem® hESC XF

– StemPro® hESC SFM

– MEF conditioned media

• Each lot qualified to ensure hPSC culture performance and reproducibility of the experiments

• Eliminates tedious maintenance of feeder cells

Corning Matrigel hESC-qualified matrix


Corning® Matrigel® matrix manufacturing and quality control

• Bedford, MA manufacturing facility ISO 9001 certified

• Production batch records, lot/raw material traceability, SOPs, and training

• Raw materials have incoming specifications and sourced from qualified vendors. Vendors are audited.

Quality Control• Tested for 27 murine viruses and

bacteria including triple redundant testing for LDEV/LDHV

• Finished goods negative for bacteria, fungi and mycoplasma, LDEV/LDHV

• Tested for endotoxin

• QC assay: Gelling stability and neurite outgrowth assay

• Custom Corning Matrigel matrix production capability to meet quality requirements of customers engaged in cell manufacturing or cell processing.


Isotype

SSEA-4

Undifferentiated hESC expansion and normal karyotype on Corning® Matrigel® hESC-qualified matrix

OCT-3/4 SSEA-4

Isotype

OCT-3/4

98% OCT-3/4 positive, 94% SSEA-4 positive

Normal karyotype after 35 passages

Study using H9 cell line in mTeSR™1 medium


H9 cells grown on Corning Matrigel matrix hESC-qualified in mTeSR™1 for >10 passages formed EBs and differentiated to cell types from all three germ layers

ECTODERM MESODERMENDODERM

Nestin/DAPI -Actin/DAPIAFP/DAPI

Tri-lineage differentiation of hESC after culture on Corning® Matrigel® matrix hESC-qualified

Embryoid bodies on Corning Ultra-Low Attachment surface plate

Corning® Synthemax®

Synthetic substrate with a Vitronectin peptide

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Corning® Synthemax®

Corning Synthemax-SC substrate– Synthetic, animal-free, defined– Self-coating by the user (culture vessel/

glass slide), easy coating protocol– US Class I medical device,

manufactured in cGMP compliant facility– Sterile– Custom pre-coated vessels available

Corning Synthemax microcarriers– Simplified scale-up processes– Sterility Assurance Level (SAL) of 10-6

– Nonpyrogenic – Available as closed system solutions


HPSC maintained undifferentiated cell markers, normal karyotype on Corning® Synthemax®-SC substrate

Study by WiCell® Research Institute 7 Passage Study

020406080

100

OCT-4 SSEA4

% P

ositi

ve

Pluripotency markers

OCT-3/4 98.9 %

SSEA-4 99.8 %

H9 cells in mTeSR™1

Normal karyotype

hiPSC in mTeSR1

Normal karyotype

Tri-lineage differentiation post-expansion

83% 93%

Corning® rLaminin-521 (human)

Recombinant protein expressed in a human cell line IS

SC

R 2015


Corning® rLaminin-521 (Human)

• Animal-free recombinant protein manufactured by BioLamina

• Supports single cell hPSC passaging without ROCK inhibitor

• Suitable for scale-up

• Compatible with multiple hPSC culture media and dissociation reagents*

• Vial for self-coating, easy coating protocol

• Filtered through 0.2 micron sterile filter

• Available in aliquots of 100 µg

*Lu, et al. Biomaterials 35:2816 (2014); Rodin, et al. Nature Comm. 5:3195 (2014).


Corning® rLaminin-521 (Human) applications

Cardiac differentiation hiPSC generation Neuronal differentiationHPSC culture

Lu, et al. Biomaterials 35:2816 (2014); Rodin, et al. Nature Comm. 5:3195 (2014);Burridge, et al. Nature Methods 11:855 (2014); WO2014087244 A2

Tyrosine hydroxylase

• HPSC culture

• Human iPSC derivation

• HPSC differentiation Neuronal cell types CardiomyocytesRetinal pigment epithelial cells

Cardiac troponin T


Corning® PureCoat™ rLaminin-521 Cultureware –coming soon

• Ready-to-use, pre-coated, animal-free, recombinant Laminin-521 (human) coating

• Supports hPSC expansion, hiPSC derivation, human neural stem cell expansion and differentiation

• Sterile, nonpyrogenic, nontoxic • 20 month stability at 2°C to 8°C, ambient shipping• USP class VI polystyrene cultureware• Lot traceable; certificate of compliance and analysis

• 6-well plates, T-75, T-175, Multi-Flask 3-Layer and 5-Layer. Corning CellSTACK® 2-, 5- and 10-Layer stacks will be available later


Corning® PureCoat™ rLaminin-521 cultureware supported xeno-free hPSC expansion, pluripotency and normal karyotype

SOX17

Otx2

BrachyuryMes

oder

mEc

tode

rmEn

dode

rm

Pluripotency markers Normal karyotype

Tri-lineage differentiation

• Gibco hiPSC line culture in NutriStem®

hESC XF medium

• 10 passage study using Accutase for dissociation

• Pluripotency markers by flow cytometry

• Tri-lineage differentiation (in vitro)

• Karyotyping

• rLaminin-521 fresh coated vessels served as control

Morphology

OCT3/4

SSEA-4

SSEA-1

Poster T-1521 June 25


hPSC scale-up in Corning® PureCoat™ rLaminin-521 Multi-Flask

0.00E+00

2.00E+05

4.00E+05

6.00E+05

T-75 flask Multiflask3-layer

6-well plate

• Easy and cost effective scale-up with consistent performance

• Unique design allows to grow more cells in the same amount of incubator space

• Even distribution of media across all layers for homogeneous cell growth

• Ability to mix cells and reagents in the Multi-Flask saves time and reduces risk of contamination

• Flexible design lets you pour or aspirate/recover cells using a pipet

Cell yield/cm2


Corning® PureCoat™ rLaminin-521 cultureware supported neural stem cell (NSC) culture and differentiation

hNSC after 4 days in culture rLaminin-521 self-coatPLO/Laminin PureCoat rLaminin-521

• H9-derived NSCs from Gibco

• StemPro® Neural Stem Cell serum-free medium

• Passaged using TrypLE™ 98.68%93.55%

99.62% 99.79%99.82%

95.49%

Sox

2N

estin

PLO/Laminin Self-coat PureCoatrLaminin-521 rLaminin-521

Pluripotency Markers

Neu

rons

(M

AP

2B)

Olig

oden

droc

ytes

(A

2B5)

Ast

rocy

tes

(GLA

ST)

Differentiation

Self-coat PureCoat Isotype1 2 3

0

20

40

60

Passage

Dou

blin

g T

ime

(hr)

Doubling Time

1 2 360

70

80

90

100

110

Passage

Cel

l Via

bilit

y (%

)

Viability

PLO/Laminin, Self-coat rLaminin-521, PureCoat rLaminin-521

Poster T-1210 June 26


Pluripotent Stem Cell Surfaces

Corning®

Matrigel®

Matrix hESC-

qualified

Corning rLaminin-521

(Human)

Corning Synthemax®

Corning’s hPSC offering: biological to synthetic The original, gold standard matrix for feeder-free hPSC expansion- Robust and compatible with defined

media environments - Industry leading manufacturing,

quality and viral testing- Broad range of formulations and

formats- Highest level of publication mentions

Defined, recombinant human Laminin-521 for simple, efficient feeder-free hPSC expansion - Robust and compatible with defined media

environments- Enables defined, single cell expansion of

hPSCs, no clump passaging needed- Low spontaneous differentiation

Synthetic, flexible self-coating substrate- Supports hPSC expansion in serum-free media - US Class I Medical Device, manufactured in cGMP

compliant facility- Easy, room temperature self-coating protocol

Pre-coated coming soon!

Corning® ECM substrates

Stem cell differentiation

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Corning® ECM substrates for stem cell differentiation

• The ability to successfully differentiate stem cells into a specific cell type with homogeneity is crucial for both basic and clinical research

• Microenvironment plays an important role in determining cell fate

• Limited progress has been made towards differentiating stem cells in a defined environment

• Corning ECM matrices can support differentiation of stem cells into specific cell types


HPSC differentiation into cell types of ectodermal origin

Higher neurite outgrowth on PDL/Laminin (LN) and Corning Matrigel matrix (MG). Ma, et al. BMC Dev Biol 8:90 (2008).

Neural progenitor

RPE markers (A) and phagocytosis (B) on Synthemax-SC Pennington, et al. Stem Cells Trans Med (2015) 4:1,

Retinal Pigment Epithelium (RPE)

PDL/Laminin Matrigel matrixLaminin-521 1

2

3

4

PDL/LamininMatrigel matrixCorning PuraMatrix™ hydrogel

Synthemax SCMatrigel matrixVitronectin

Synthemax surface

hPSC

5 PDL/LamininMatrigel matrix

1. Yao, et al. PNAS (2005) 103:6907; Boissart, et al. Transl Psychiatry (2013) 3:e294; Lu, et al. Biomaterials (2014) 35:2816.

2. Liedmann, et al. Bioreseach open access (2012) 1:16; Thonhoff, et al. Brain Res (2008) 1187:42.

3. Li, et al. Stem Cell Dev (2013) 22:823.4. Sridhar, et al. Stem Cells Transl Med (2013) 2:16; Zahabi et al. Stem Cells &

Dev (2012) 21:2262; Zhu et al. PLOS ONE (2013) 8:e54552; Pennington et al. Stem Cells Trans Med (2015) 4:1.

5. Nistor, et al. PLOS ONE (2011) 6:e20692; MA, et al. BMC Dev Biol (2008) 8:90.


HPSC differentiation into cell types of mesodermal origin

Cardiomyocytes on Corning® Matrigel® Matrix

Differentiation to cardiomyocytes on Corning Matrigel matrixsandwich.Zhang, et al. Circulation Research 111:1125 (2012).

1

Matrigel matrix Matrigel matrix

2

Matrigel matrixLaminin-521

3

hPSC

1. Park, et al. Blood 16:5762 (2012). 2. Joo, et al. Blood 120:2733 (2012) .3. Zhang, et al. Circ Res 111:1125 (2012); Burridge, et al. Nature

Methods 11:855 (2014).


HPSC differentiation into cell types of endodermal origin

Differentiation to Hepatocytes on Corning® Matrigel® Matrix

Cai, et al. (2012), StemBook, ed. The Stem Cell Research Community, StemBook, doi/10.3824/stembook.1.52.1.

2

Matrigel matrix

1

Matrigel matrixCollagen I

3

Matrigel matrix

hPSC

1. Chen, et al. Nature Chem Biol 5:258 (2009). 2. Zhao, et al. PLOS ONE 4:e6468 (2009). 3. Wang, et al. PNAS 104:4449 (2007).


Summary

• Corning provides a breadth of matrices for hPSC derivation, expansion, and differentiation to meet researcher’s need.

• Corning ECM matrices support undifferentiated hPSC expansion for multiple passages, hPSC maintained pluripotency and normal karyotype.

Corning® Matrigel® matrix: robust and cost effective matrix

Corning Synthemax®: defined, synthetic, easy to self-coat

Corning rLaminin-521 (Human): animal-free, recombinant matrix, easy to self-coat, supports ROCK inhibitor-free single cell passage

• Corning ECM matrices support differentiation of stem cells into specific cell types.

• Corning PureCoat™ rLaminin-521 cultureware will provide ready to use option for hPSC and hNSC culture and scale-up.


Contact information

For additional product or technical information, visit www.corning.com/lifesciences

or call Scientific Support at 1.800.492.1110 (United States)

Outside the United States, call 978.442.2200 or contact your local Corning sales office.

For a listing of trademarks, visit us at www.corning.com/lifesciences/trademarks.All other trademarks in this document are the property of their respective owners.© 2015 Corning Incorporated. All rights reserved.

corning innovation showcase€¦ · paula flaherty technology manager deepa saxena, ph.d. senior...

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