cosmosil hplc columns - winlab · 2020. 4. 28. · cosmosil® hplc columns sample separation mode...

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COSMOSIL® HPLC Columns

Sample Separation Mode

Packing Material

USP Code

Stationary Phase

Special Features and Applications

Organic Compounds (low M.W.)

Reversed Phase

3C18-EB L1 Octadecyl Group Excellent for basic compounds

3 µm silica packing material

C18-MS-II L1 Octadecyl Group Multi-purpose C18column Monofunctional silylation

on ultra-pure silica gel for separation of the widest range of compounds

C18-AR-II L1 Features strong acid resistance and suitable for acidic compounds and peptides

C18-PAQ L1 Reversed phase column, compatible with 100% water based mobile phase.

Suitable for hydrophilic compounds

Cholester L101 Cholesteryl Group

Usable under the same condition as C18

Unique rigid cholesteryl structure improves separation

PBr - Pentabromo-benzyl group

Separate hydrohilic compounds in reversed-phase conditions

πNAP - Naphtylethyl Group

Stronger π-π interaction than Phenyl column

PYE - Pyrenylethyl Group

Most powerful π-π interaction

NPE - Nitrophenylethyl Group

Separation utilizing π-π interaction and dipole-dipole interaction

PBB-R - Pentabromo-benzyl Group

Separation utilizing dispersion force

PFP L43 Pentafluoro-phenyl Group

Separation by dipole-dipole interactions.

CN-MS L10 Cyanopropyl Group

Great reproducibility using isocratic elution mode

C22-AR-II - Docosyl Group

AdminText BoxAustralia & New Zealand contact: [email protected] or call +61 (0)7 3205 1209



Packing Material

USP Code

Stationary Phase


C8-MS L7 Octyl Group Alkyl chain columns except C18 column C4-MS L26 Butyl Group

TMS-MS L13 Trimethyl Group

PE-MS L11 Phenylethyl Group

π-π interaction

Normal Phase

SL-II L3 - Suitable for preparative separation

Hydrophilic Interaction

HILIC L104 Triazole Retains highly polar compounds that would not be retained in C18column

Mono-saccharides Oligo-saccharides

Hydrophilic Interaction

Sugar-D - Secondary/ Tertiary Amine

Suitable for monosaccharides, oligosaccharides

NH2-MS L8 Aminopropyl Group

Primary amine bonded column

Proteins Reversed Phase

Protein-R L1 Octadecyl Group Provides improved separation, high recovery rate, and acid resistance

C18-AR-300 L1 Octadecyl Group Wide pore type reversed phase columns

C8-AR-300 L7 Octyl Group

C4-AR-300 L26 Butyl Group

Ph-AR-300 L11 Phenyl Group

Proteins Gel Permeation

Diol-120-II L20 Diol Group Silica-based gel filtration column

Sample MW (Protein) 5,000-100,000 Da

Diol-300-II Silica-based gel filtration column

Sample MW (Protein) 10,000-700,000 Da

Diol-1000-II

Silica-based gel filtration column

Sample MW (Water Soluble Polymer) 50,000 - 500,000 Da

Ion-exchange

Type Q Quaternary Ammonium Type

Anion-exchange Type - For Purification

Target Sample: Acidic Proteins / DNA

Type Q-N Anion-exchange Type For Ultra-fast Analysis,

Precise Analysis




Packing Material

USP Code

Stationary Phase


Target Sample: Acidic Proteins / DNA

Type S Sulfopropyl Type Cation-exchange Type For Purification Target Sample: Basic Proteins

Type S-N Cation-exchange Type - For Ultra-fast Analysis, Precise Analysis - Target Sample: Basic Proteins

Type M Quaternary Ammonium Type/Sulfopropyl Type

Amphoteric ion-exchange Type - For Purification - Target Sample: All Proteins

Type M-N Amphoteric ion-exchange Type - For Precise Analysis - Target Sample: All Proteins

Hydrophobic Interaction

HIC - - Suitable for separation of peptide, and there is only a little less in enzyme activity and the teriary structure of proteins

Fullerenes - Buckyprep - Pyrenylpropyl Group

Standard column for fullerene separation

Buckyprep-D

- Nitro-carbazoyl Group

Designed for separation of fullerene derivatives

Buckyprep-M

- Phenothiazinyl Group

Designed to separate metallofullerenes

PBB - Pentabromo-benzyl Group

Designed for preparative separation of C60, C70

PYE - Pyrenylethyl Group

Separation of Fullerenes and structural isomers

NPE - Nitrophenylethyl Group

Separation of derivatived fullerene

Carbon Nanotubes

Gel Permeation

CNT Hydrophilic Group

Separation of carbon nanotubes



Selection Guide

Organic Compounds (Low M.W.) Octadecyl group bonded column (C18ODS) are recommended as first-choice columns for separations of organic compounds (Low M.W.). If there is not enough separation or no retention using COSMOSIL C18 columns, COSMOSIL series offer many kinds of special columns.

Please click here for the selection guide for C18 columns

Saccharides COSMOSIL Sugar-D is recommended for the separation of monosaccharides and

oligosaccharides as a first-choice column. For the separation of sugar derivatives, COSMOSIL C18-PAQ is suitable as well. Proteins Please select based on the separation mode. Fullerenes COSMOSIL Buckyprep is most suitable for the separation of fullerenes. COSMOSIL Specialty Columns Click here



USP Specifications

The USP (U.S. Pharmacopeia) specifications are listed below with the recommended COSMOSIL HPLC columns.

USP Code

Description Recommended Columns

L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.7 to 10 m in diameter, or a monolithic rod

COSMOSIL 3C18EB COSMOSIL C18-MS-II COSMOSIL C18-AR-II COSMOSIL C18-PAQ COSMOSIL C18-AR-300 COSMOSIL Protein-R

L3 Porous silica particles, 5 to 10 µm in diameter COSMOSIL SL-II

L7 Octylsilane chemically bonded to totally porous silica particles, 1.7 to 10 µm in diameter

COSMOSIL C8-MS COSMOSIL C8-AR-300

L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod.

COSMOSIL NH2-MS

L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter

COSMOSIL CN-MS

L11 Phenyl groups chemically bonded to porous silica particles, 1.7 to 10 µm in diameter

COSMOSIL PE-MS COSMOSIL Ph-AR-300

L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diamete

COSMOSIL TMS-MS

L20 Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm in diameter

COSMOSIL Diol-120-II COSMOSIL Diol-300-II COSMOSIL Diol-1000-II

L26 Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter

COSMOSIL C4-MS COSMOSIL C4-AR-300

L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter

COSMOSIL PFP

L101 Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.

COSMOSIL Cholester COSMOCORE Cholester

L104 Triazole groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter

COSMOSIL HILIC

Distributor in Australia and New Zealand: WINLAB PTY LTD 2 Pinacle St, Brendale, Queensland, Australia, 4500 PO Box 5007, Brendale, Queensland, Australia, 4500 Ph: +61 7 3205 5233: Fax: +61 7 3205 1209 Email: [email protected] www.winlab.com.au

AdminText BoxDistributed in Australia and New ZealandWINLAB PTY LTDPO Box 5007Brendale, Queensland, Australia 4500Ph: +61 (0)7 3205 1209 Email: [email protected] Web: www.winlab.com.au

cosmosil hplc columns - winlab · 2020. 4. 28. · cosmosil® hplc columns sample separation mode...

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