I

Industrial Biotechnology for Lignocellulose Based processes

-Course Program-

Sunday October 22nd Introduction, raw material composition and sources Time Title N.1 By 9:00-9:45 Course introduction. Round the table presentations LO

9:45-10:00 Coffee

10:00-10:45 Bioethanol and the development towards the concept of biorefining – an overview

1 LO

10:45-11:15 Plant cell wall fundamentals 2 JL

11:15-12:00 Basic raw material analytics: HPLC, HPAEC, reducing sugars 3 LO

12:00-13:00 Lunch

13:00-13:45 Algal biorefineries: overview and perspectives 4 JM

13:45-14:30 Fractionation and pre-treatment methods 5 MG

14:30-15:00 Coffee

15:00-16:00 New biorefinery concepts with ionic liquids and deep

eutectic solvents 6 RW

16:00-17:00 Bioenergy and biomaterials sustainability issues 7 CC

17:00-18:00 Exercises and discussion

18.00-18.15 Presentation of the BIO department at Chalmers Stefan Hohmann, Head of Department

Dinner & Student poster presentation, get-together

Monday October 23rd Characterization of lignocelluloses Time Title N.1 By

9:00-9:45 Challenges in sample preparation and analysis of

lignocellulose polymeric materials 8 MH

9:45-10:30 Enzymatic hydrolysis of lignocellulose - introduction 9 KSJ

10:30-10:45 Coffee

10:45-12:00 Enzyme discovery for biorefinery applications 10 JL

12:00-13:00 Lunch

13:00-14:15 Mass spectrometric-based glycomic analysis of lignocellulosic

materials 11 FVD

14:15-14:45 Coffee

14:45-15:45 Business aspects in biorefinery development 12 KSJ

15:45-16:30 Algal biorefineries: extractable products and applications 13 EA/JM

16:30-18:00 Exercises and discussion

18:00 Dinner

II

Tuesday October, 24th Enzymes and their action Time Title N.1 By 9:00-9:45 Enzyme classification and the CAZY database 14 JL

9:45-10:30 Cellulolytic enzymes 15 LO

10:30-10:45 Coffee

10:45-12:00 Lytic Polysaccharide Monooxygenases (LPMOs) and other redox enzymes in polysaccharide degradation – fundamental and applied aspects

16 VE

12:00-13:00 Lunch

13:00-14:15 Mannolytic and xylanolytic enzymes 17 KBK

14:15-14:45 Coffee

14:45-15:45 Lignin acting enzymes 18 KH

15:45-16:30 Bacterial anaerobic degradation of biomass 19 JL

16:30-17.30 Exercises and discussion

18:30 Course Dinner downtown Gothenburg S/S Marieholm

Wednesday October 25th Microorganisms Time Title N.1 By

9:00-9:45 Different products of microbial metabolism and thermodynamic driving forces

20 CJF

9:45-10:30 Inhibitors in lignocellulose hydrolysates 21 LO

10:30-10:45 Coffee

10:45-12:00 Strain improvement by mutagenesis, directed evolution and screening

22 YN

12:00-13:00 Lunch

13:00-13:45 Strain improvement by metabolic engineering 23 YN

13:45-14:30 Development of industrial yeast strains 24 YN

14:30-15:00 Coffee

15:00-16:00 Bacterial Cell Factories 25 RvK

16:00-16.45 Enzyme production by filamentous fungi: from secretion to large scale production

26 LO

16:45-18.00 Exercises and discussion

18:00 Pizza at the department and lab visit

III

Thursday October 26th Fermentation processes Time Title N.1 By 9:00-9:45 Cell propagation 27 LO

9:45-10:30 Fermentation basics: important fermentation variables and

mode of operation of bioreactors 28 CJF

10:30-10:45 Coffee

10:45-11:30 Fermentation basics: important fermentation variables and mode of operation of bioreactors - continued

28 CJF

11:30-12:00 Lignocellulose hydrolysate fermentation: microbial robustness

29 LO

12:00-13:00 Lunch

13:00-13:45 Fermentation of lignocellulosic media: mode of operation and strategies

30 CJF

13:45-14:30 Quantitative evaluation of fermentations of lignocellulose hydrolysate

31 RK

14:30-15:00 Coffee

15:00-15:45 Downstream processing 32 RH-K

15:45-16.30 Microbial electrofermentations 33 NX

16:30-17.30 Exercises and discussion

17:30-18.00 Concluding discussions, questions and sum-up

18:00 Dinner

Friday October 27th Exam and seminars 9:00-11:00 Written exam 11:00-11:30 Coffee

SEMINARS: HOT TOPICS IN INDUSTRIAL BIOTECHNOLOGY

11:30-12:00 Perspectives, challenges and trends towards biorefineries and a new bioeconomy Lisbeth Olsson – Chalmers University of Technology

12:00-13:00 Lunch

13:00-13:45 St1 Cellunolix® process - ethanol and other products from forest residues Tom Granström, St1 Renewable Energy

13:45-14:30 Production of fine chemicals through bioconversion of lignin Magnus Carlquist, Lund University

14:30-15:00 Coffee

15:00-15:45 Gas fermentation of CO2, hydrogen, syngas and methane Juha-Pekka Pitkänen, VTT Technical Research Centre of Finland

15:45-16.30

Single molecule evidence on enzymatic cellulose degradation from experiment and modeling Bernd Nidetzky, Graz University of Technology

16:30-17.00 Course evaluation and concluding remarks

IV

Contacts: Lisbeth Olsson (Course Responsible) lisbeth.olsson@chalmers.se Yvonne Nygård yvonne.nygard@chalmers.se Maurizio Bettiga maurizio.bettiga@chalmers.se

Notes 1) N. refers to “Subject Number” in the course literature list

Course lecturers: Chalmers University of Technology; Gothenburg, Sweden Lisbeth Olsson, Carl Johan Franzén, Eva Albers, Johan Larsbrink, Yvonne Nygård, Christel Cederberg, Joshua Mayers, Merima Hasani, Rakesh Koppram, Nikolaos Xafenias Corbion, Wageningen University and Research, The Netherlands Richard van Kranenburg Graz University of Technology, Austria Bernd Nidetzky Lund University, Sweden Mats Galbe, Rajni Hatti-Kaul, Magnus Carlquist Novozymes A/S, Bagsvaerd, Denmark Kristian Bertel Rømer Mørkeberg Krogh Norwegian University of Life Sciences, Ås, Norway Vincent Eijsink Royal Institute of Technology, Stockholm, Sweden Francisco Xavier Vilaplana Domingo St1 Renewable Energy, Finland Tom Granström

University of Copenhagen, Denmark Katja Salomon Johansen University of Helsinki, Finland Kristiina Hildén VTT Technical Research Centre of Finland Ltd, Finland Juha-Pekka Pitkänen, Ronny Wahlström