crime scene investigation. summary 1.haemoglobin - the unique compound of blood 2.techniques of...
TRANSCRIPT
CHEMISTRY IN C.S.I.
IS THE BLOOD ALWAYS RED?
CRIME SCENE INVESTIGATION
FACULTY OF CHEMICAL AND FOOD TECHNOLOGY
SLOVAK UNIVERSITY OF TECHNOLOGY
MARTINA STANKOVA 1B GROUP 57
SUMMARY
1.Haemoglobin - the unique compound of blood
2.Techniques of blood’s detection
Luminescene
Crystal reaction
Catalytic color reaction
Instrumental reaction
3.Resume of detection techniques
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED? 2
HAEMOGLOBIN
Structure of human haemoglobin.
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
α protein's subunits
β protein's subunits
iron-containing heme
groups
3
4
TECHNIQUES OF BLOOD’S DETECTION
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
Technique based on
Heme stain
Luminescence Luminol/LumisceneFluorescein
Crystal test Takayama test
Catalytic color test
Leucocrystal violetKastle-Meyer test
Instrumental test
High performance liquid chromatography
Proteins stain
Catalytic test Amido black
CHEMILUMINESCENCE OF LUMINOL
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
+ hν 425 nm
1
5
LUMINOL – EMISSION SPECTRUM
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
Detection of blood using Luminol on crime sceneLuminol emission spectrum : peak at
425 nmHuman eye range : from 380 nm to 780 nm
6
LUMISCENE – IMPROVED LUMINOL
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
Lumiscene’s emisson spectrum – peak at 525 nm
Same reaction but better wavelenght and eye sensitivity
7
Color corresponding to wavelenght of 525 nm
KASTLE-MEYER TEST - PHENOLPHTHALIN
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED? 8
NEGATIVE/POSITIVE KASTLE-MEYER TEST
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED? 9
Negative and positive result to presence of blood
RESUME
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED?
Compound Use Color Sensitivity Persistance Interference Damage
Luminol Reagent for latent bloodstains
Blue glow
0,0001 ml of blood
Photographs should be taken within a few hours
Copper salts, brass, bronze and other similar alloys
No damage
Kastle-Meyer test
Reagent for latent and visible bloodstain
Pink 0,000 001 ml of blood
Immediate change of color
Vegetable peroxidases, copper, nickel salts
Damage of DNA
LCV Latent prints, deposited blood on non-porous surfaces,visible blood prints
Purple 0,001 ml of blood
Immediate change of color
Copper, nickel and other similar salts
Damage of DNA
Takayama test
Reagent for latent bloodstain
Pink crystals
0,001 ml of blood
Formation of crystals
none Damage of DNA
Fluorescein Reagent for latent bloodstains
Greenfluores-cence
0,0001 ml of blood
Photographs should be taken within a few hours
Copper, nickel and other similar salts
No damage
Amido black
Latent prints and to enhance visible blood prints on painted surfaces
Dark blue
0,001 ml of blood
Photographs should be taken within a few hours
Damage of blood sequence
10
Compound Use Color Sensitivity Persistance Interference Damage
Luminol Reagent for latent bloodstains
Blue glow
0,0001 ml of blood
Photographs should be taken within a few hours
Copper salts, brass, bronze and other similar alloys
No damage
Kastle-Meyer test
Reagent for latent bloodstain
Pink 0,000 001 ml of blood
Immediate change of color
Vegetable peroxidases, copper, nickel salts
Damage of DNA
LCV Latent prints, deposited blood on non-porous surfaces,visible blood prints
Purple 0,001 ml of blood
Immediate change of color
Copper, nickel and other similar salts
Damage of DNA
Takayama test
Reagent for latent bloodstain
Pink crystals
0,001 ml of blood
Formation of crystals
None Damage of DNA
Fluorescein Reagent for latent bloodstains
Greenfluores-cence
0,0001 ml of blood
Photographs should be taken within a few hours
Copper, nickel and other similar salts
No damage
Amido black
Latent prints and to enhance visible blood prints on painted surfaces
Dark blue
0,001 ml of blood
Photographs should be taken within a few hours
Damage of DNA
THANK YOU FOR YOUR ATTENTION
QUESTIONS?
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED? 11
REFERENCES
• Dr R V Winchester, XII-Biotech-A-Blood Detection-6;
• O. Germain, C. Fregeau, and R.M. Fourney, Evaluation of the Effects of Blood Enhancement Chemicals on the Subsequent Short Tandem Repeat DNA Typing Analysis of Blood Fingerprints;
• Shannon M. Casey, Hilary M. Stinnett, Gory Dactyloscopy;
• http://www.evidentcrimescene.com/cata/chem/chem.html
• http://www.lumiscene.com/indexa.html
• http://www.sciencebuddies.org/science-fair-projects/project_ideas/BioChem_p037.shtml
• http://www.inreferencetomurder.com/html/crime_scene.html
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY
CHEMISTRY IN C.S.I.IS THE BLOOD ALWAYS RED? 12