Maintrac®

Maintrac liquid biopsy cell staining allows quantitative detection of vital circulating tumor cells

NO fixation.

NO isolation.

NO enrichment.

Fluorochrome labeled antibody FITC

Circulating tumor cell

Surface antigen EpCAM

Cellsearch®

Fixation and enrichment procedures leads to

LOSS of cells

LOSS of antigenicity.

Cells are DEAD

Magnetic beads –(cells with low EpCAM expression can not be cought by magnet)

Circulating tumor cell (dead cell due to fixation)

Fixation - (destroys part of surface antigens, including EpCAM)

Comparison Maintrac vs CellSave

y = 0,1898x - 450,44

R2 = 0,9191

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total events Maintrac

tota

l eve

nts

Cel

lSav

e

Duplicate analysis in one patient, one blood sample

Maintrac® results

Cellsearch results

Cellsearch results

S poor and good prognosis derived from the same blood sample from the same patient at the same time ...

Comparison WebmedCentral CANCER 2011;2(2):WMC001490

Comparing Sequential Steps For Detection Of Circulating Tumor Cells: More Specific Or Just Less Sensitive?

Other CTC technologies

Technique

Problems

Magnetic beat enrichment Is EpCam expression sufficent for enrichment?

Microfiltration Are all circulating tumor cells larger than blood cells?

Negative Depletion Are all circulating tumor cells CD45 negative?

Adhesion to micropoles Technical problems?

CETC comparison to ctDNA

Technique

Problems

Isolation from plasma DNA derived from destroyed cells. Chemosensitivity on vital cells not possible.

Derived from dead cells Stability of tumor DNA

Mutation analysis Additional mutations due to DNA degradation