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CURRICULUM VITAE (CV)
First name Behrooz
Surname Johari
Date, Place of Birth MAR 4, 1987; Nahavand, Hamedan
Province
Marital Status Married, one children
Nationality Iranian
CONTACT PROFESSIONAL ADDRESSES
Address:
Assistant Professor
Dept. of Medical Biotechnology
Zanjan University of Medical Sciences
Mahdavi Blvd., Shahrak Karmandan
Zanjan 45139-56111, IRAN
Mobile: (0098) 939 3724494 E-mail: [email protected]
QUALIFICATION
PhD in Medical Biotechnology, (2012-2017) Project title: Using c-Myc transcription factor
oligodeoxynucleotide decoys to inhibit stemness in mouse embryonic stem cells, Department of
Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
MSc in Medical Biotechnology, (2010-2012) Project title: Study of the effects of a gelatin-
hydroxyapatite Nano-composite with osteoblast and endothelial cells in the management of an
experimental model of critical bone defect in rats, Department of Medical Biotechnology, Faculty of
Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran.
BSc in surgical Technology (Operating room), (2008-2010) Department of surgical Technology,
Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Associate degree in surgical Technology (Operating room), (2006-2008) Department of
surgical Technology, Faculty of Allied Medicine, Kermanshah University of Medical Sciences,
Kermanshah, Iran.
Diploma in Experimental Sciences, (2003-2006) Shahid Beheshti High School, Nahavand,
Hamedan Province, Iran.
HONORs and AWARDs
1. Membership in Bonyad Nokhbegan of Iran from 2017.
2. Top Student in the class medical biotechnology, Tehran University of Medical Sciences,
Tehran, Iran (2012).
3. Top Student among students of Faculty of Allied Medicine, Iran University of Medical
Sciences, Tehran, Iran (2010).
4. Talented Student in Faculty of Allied Medicine, Iran University of Medical Sciences,
Tehran, Iran (2008-2010).
5. Talented Student in Faculty of Allied Medicine, Kermanshah University of Medical
Sciences, Kermanshah, Iran (2006-2008).
6. Top Student in the class Experimental Sciences at shahid beheshti highschool, Nahavand,
Hamedan, Iran (2002-2006).
INTERESTS
Tissue engineering, Cancer therapy, Cell signaling, Differentiation therapy, Drug delivery.
RESEARCH EXPERIENCES and SKILLS
In vitro, Cell culture (stem cell/cell line), Tissue preparation for Histology,
Immunohistochemistry (IHC), Light, fluorescent and confocal microscopy, MTT test, Tissue
engineering scaffold synthesis, RNA & DNA extraction, PCR, Real- time PCR, Electrophoresis
(SDS PAGE), EMSA, Western blotting etc.
In vivo, Animal working include handling, injection and surgery.
Analytical techniques: Bioinformatics tools (primer3, oligoanalyzer), Statistics Software
(Microsoft Excel, Graph Pad Prism…), ICDL etc.
Cellular and molecular biology
Cell culture technics,
Principal of Animal working
Medical biochemistry
Principal of researches
Information system in medicine
Biosafety in laboratory
Bioethics in researches
Medical biotechnology,
Molecular biotechnology
Introduction of Nanobiotechnology
TEACHING COURSES
PUBLICATIONS
BOOKS (In Farsi)
Images of Book
Title of Books
Publisher and date of publication
Medical Biotechnology
Bernard Glick
Under publishing
Immunology Under publishing
Immunology For Pharmacy -Dennis K.
Flaherty- 2012
Eshraghie (2016)
Gene Cloning & DNA Analysis Eshraghie (2017)
An introduction to 3D cell culture animal
cell& tissue engineering
Eshraghie (2017)
Molecular biotechnology- Bernard R Glick,
Jack J Pasternak-2010
Eshraghie (2016)
Essential of Cellular & Molecular Biology&
Genetic Engineering
Eshraghie (2014)
Q&A of Cellular& Molecular Biology&
Genetics Engineering
Eshraghie (2015)
Principal dimensional culture of animal cells Eshraghie (2016)
Clinical Biochemistry (DRS) Jameenegar (2015)
Practical Principle of Microbiology
Laboratory
Jameenegar (2015)
Guideline for laparoscopy& Thoracoscopy
surgery
Khosravi (2012)
Surgical Technologies & Scrub Andishe-rafi (2012)
JOURNALS (Full text in English)
1. Meghdad Abdollahpour‐Alitappeh, Majid Lotfinia, ..., Behrooz Johari, …., Antibody–drug
conjugates (ADCs) for cancer therapy: Strategies, challenges, and successes. 2018, J Cell
Physiol, DOI: 10.1002/jcp.27419.
2. Maryam Farshadi, Behrooz Johari, …, Ali Samadikuchaksaraei. Evaluation of
nanocomposite scaffold seeded with mesenchymal stem cells for bone repair. 2018, cell biology
international, accepted.
3. Leila Saremi, Shirin Lotfipanah, …, Behrooz Johari, …, The Pro12Ala polymorphism in
the PPARγ2 gene is not associated with an increased risk of NAFLD in Iranian patients
with type 2 diabetes mellitus. 2018, cellular and molecular biology letter, accepted.
4. Leila Saremi, Shirin Lotfipanah, …, Behrooz Johari, …, Association of Superoxide
Dismutases (SOD1) Gene Polymorphisms Synergistically with Development of Diabetic
Retinopathy (DR) and Cataract in Iranian Type ΙΙ Diabetes Mellitus Patients. 2018, cellular
and molecular biology, accepted.
5. Mohsen Mousavi, Behrooz Johari, Jamil Zargan, … , Antibacterial effects of the
Latrodectus dahli crude venom on Escherichia coli, Staphylococcus aureus and Bacillus
subtilis. Medical Laboratory Journal, 2018, accepted.
6. Behrouz Farhadihosseinabadi, Fahimeh Hosseini, …, Behrooz Johari. Breast Cancer:
Risk Factors, Diagnosis and Management. Medical Laboratory Journal, Sep-Oct, 2018; Vol 12:
No 5.
7. M Kazemi, M Azami, B Johari, M Ahmadzadehzarajabad, B Nazari. Bone Regeneration
in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial
cells (HUVEC s). 2018, International Journal of Applied Ceramic Technology, in press.
8. Ali Rahiminejad, Rassoul Dinarvand, Behrooz Johari, …, investigation and preparation
of Indirubin-loaded SLN nanoparticles and their anti-cancer effects on human
glioblastoma U87MG cells. 2018, cell biology international, in press.
9. Saeed Dezianian, Jamil Zargan, … , Behrooz Johari. In vitro Study of Hottentotta
Schach Crude Venom Anticancer Effects on MCF-7 and Vero Cell Lines. 2018, Iranian
journal of pharmaceutical research, accepted.
10. Mohsen Mousavi, Jamil Zargan, … , Behrooz Johari. Anticancer effects of the
Latrodectus dahli crude venom on MCF-7 breast cancer cell line. 2018, breast journal,
accepted,
11. S Jahandideh, F Maghsood, NM Ghahhari, …, B Johari. The effect of Trimetazidine
and Diazoxide on immunomodulatory activity of human embryonic stem cell-derived
mesenchymal stem cell secretome. 2017, Tissue and Cell 49 (5), 597-602.
12. Behrooz Johari, Jamil Zargan. Simultaneous targeted inhibition of Sox2-Oct4
transcription factors using decoy oligodeoxynucleotides to repress stemness properties
in mouse embryonic stem cells. 2017, Cell biology international,41 (12), 1335-1344.
13. Adel M, Zahmatkeshan M, Johari B, Kharrazi S, Mehdizadeh M, Bolouri B, Rezayat
SM. Investigating the effects of electrical stimulation via gold nanoparticles on in vitro
neurite outgrowth: Perspective to nerve regeneration. Microelectronic Engineering. 2017 Apr
5; 173:1-5. (Impact Factor:1.8)
14. Johari B, Ebrahimi-Rad M, Maghsood F, Lotfinia M, Saltanatpouri Z, Teimoori-Toolabi
L, Sharifzadeh Z4, Karimipoor M3, Kadivar M. Myc Decoy Oligodeoxynucleotide
Inhibits Growth and Modulates Differentiation of Mouse Embryonic Stem Cells as a
Model of Cancer Stem Cells. Anticancer Agents Med Chem. 2017 Apr 12. doi:
10.2174/1871521409666170412142507. (Impact Factor:2.7)
15. Behrooz Johari, Mehdi Kadivar, Shirin Lak, Mazaher Gholipourmalekabadi, Aleksandra
M. Urbanska, Masoud Mozafari, Maryam Ahmadzadehzarajabad, Asaad Azarnezhad,
Samane Afshari, Jamil Zargan, Saeid Kargozar, Osteoblast–seeded bioglass/gelatin
nanocomposite: a promising bone substitute in critical-size calvarial defect repair in
rat, Int J Artif Organs 2016; 39(10): 524-533. (Impact Factor:1.005)
16. Zohreh Saltanatpour, Mehdi Kadivar, Behrooz Johari, Majid Lotfinia, Faezeh Maghsood,
Sirous Zeinali, Leila Nasehi, Ali Fallah, Aslan Fanni, Behrooz Nikbin, Transduction of an
optimized recombinant lentivirus expressing E-cadherin shRNA resulted in stable
downregulation of CDH1 gene and obvious cell morphological change in the human colorectal
cancer cell line HT29, Health Sciences 5.11 (2016): 87-93.(Impact Factor: 0.05)
17. A Azarnezhad, Z Sharifi, R Seyedabadi, A Hosseini, B Johari, Cloning and Expression of
Soluble Recombinant HIV-1 CRF35 Protease-HP Thioredoxin Fusion Protein, Avicenna
Journal of Medical Biotechnology 8.4 (2016): 175. (Impact Factor: 0.5)
18. Behrooz Johari, Maryam Ahmadzadehzarajabad, Mahmoud Azami, Mansure Kazemi,
Mansooreh Soleimani, Saied Kargozar, Saieh Hajighasemlou, Mohammadmorad
Farajollahi, Ali Samadikuchaksaraei, Repair of Rat Critical Size Calvarial Defect Using
Osteoblast-like and Umbilical Vein Endothelial Cells seeded in Gelatin/Hydroxyapatite
Scaffolds, J Biomed Mater Res A. 2016 Mar 17. DOI:10.1002/jbm.a.35710. (Impact Factor:3.5)
19. Ali Samadikuchaksaraei, Mazaher Gholipourmalekabadi, Elham Erfani Ezadyar,
Mahmoud Azami, Masoud Mozafari, Behrooz Johari, Saeid Kargozar, Seyed
Behnamedin Jameie, Alireza Korourian and Alexander M. Seifalian, Fabrication and In
vivo evaluation of an osteoblast-conditioned nano-hydroxyapatite/gelatin composite
scaffold for bone tissue regeneration, J Biomed Mater Res A. 29 MAR 2016. DOI:
10.1002/jbm.a.35731. (Impact Factor: 3.5)
20. Saieh Hajighasemlou, Mahmoud Alebouyeh, Hossein Rastegar, Mojgan Taghizadeh
Manzari, Milad Mirmoghtadaei, Behjat Moayedi, Maryam Ahmadzadeh, Farzad
Parvizpour, Behrooz Johari, Maria Moslemi Naeini, Mohammad M Farajollahi,
Preparation of Immunotoxin Herceptin-Botulinum and Killing Effects on Two Breast
Cancer Cell Lines, APJCP.2015.16.14.5977.(Impact Factor: 2.5)
21. Lotfinia, M., Lak, S., Mohammadi Ghahhari, N., Johari, B., Maghsood, F., Parsania, S. &
Kadivar, M., Hypoxia Pre-Conditioned Embryonic Mesenchymal Stem Cell Secretome
Reduces IL-10 Production by Peripheral Blood Mononuclear Cells. 2016, Iranian biomedical
journal.(Impact Factor: 0.0)
22. Fatemeh Nasiri, Behrooz Johari, Fatemeh Amiri, Mehryar Habibi Roudkenar, Sedigheh
Molaei, Marzie Bahadori, Marjan Movahed, Abazar Yari, Mohammad Ali Jalili, H2O2-
preconditioned umbilical cord-derived mesenchymal stem cells ameliorate liver regeneration in
acute liver failure-induced mice. Anatomical Sciences.2016; 13(3):183-190.
RESEARCH PROJECTS
1. The study of the inhibition effect of Tcf3 transcription factor using oligodeoxynucleotide
decoy on stemness master regulatory genes in mouse embryonic stem cells. (Grant No: A-
12-1244-3)
2. Investigation of complex formation between OCT4 transcription factor and designed
oligodeoxynucleotide decoy regulatory E-box in HT29 cell line containing enriched cancer
stem cells. (Grant No: A-12-1244-1)
3. Design and synthesis of decoy oligodeoxynucleotides for OCT4 and SOX2 transcription
factors and investigation of its antitumor effects on HT29 cell line containing enriched
cancer-like stem cells. (Grant No: A-12-1244-2)
4. Using c-Myc transcription factor oligodeoxynucleotide decoys to inhibit stemness in
mouse embryonic stem cells. (code: 730)
5. Bone healing effects of a tissue engineered gelatin hydroxyapatite Nano- composite
seeded with osteoblast and endothelial cells in the management of an experimental modal
of critical size- bone defect in rat. ) code: 16240)
6. An osteoblast seeded gelatin hydroxyapatite Nano- composite for the repair of an
experimental modal of critical size- bone defect. (code: 16234)
7. Enhancement of bone healing properties of a Bioglass-gelatin composite with endothelial
cells. (code: 16227)
8. Investigation the effects of electrical stimulation via gold nanoparticles for in vitro nerve
regeneration. (code: 18035)
WORKSHOPS ATTENDED
(2018گارگاه تجزیه تحلیل آماری داده های پژوهشی، دانشگاه علوم پزشکی زنجان.) .1
(2018کارگاه کالس درس وارونه، دانشگاه زنجان. ) .2
(2018علمی، دانشگاه علوم پزشکی زنجان. )کارگاه نگارش و انتشار مقاالت .3
(2018دانشگاه علوم پزشکی زنجان. ) کارگاه دانش افزایی با موضوع تمدن نوین اسالمی، .4
( 2018دانشگاه علوم پزشکی زنجان. ) کارگاه دانش تعامل موثر) اهمال کاری و راه های مقابله با آن(، .5
6. CRISPR/Cas9 Genome Editing Technology, university of Zanjan (2018).
7. CLC main workbench, Pasteur institute of Iran (2016).
8. Antibody design workshop, which held by Rastegar reference laboratory of faculty of
veterinary medicine, university of Tehran (2016).
9. Immunoinformatics workshop, which held by EMGEN in collaboration with HBC (2014).
10. Chemoinformatics workshop, which held by EMGEN in collaboration with HBC (2014).
11. Bioinformatics as title education oligo7& allele ID Software workshop, Pasteur institute
of Iran (2014).
12. Flowcytometry workshop, Pasteur institute of Iran (2015).
13. GMP (Good Manufacturing Practice) and GLP (Good Laboratory Practice) workshop,
Pasteur institute of Iran (2015).
14. Based on criticism reflection in medical science, Iran university medical science (2010).
15. Medical education according to best observation, Iran university medical science (2010).
16. Student network of EBM, Iran university medical science (2010).
RECENT SEMINAR AND CONGRESS
1. 1397 (2018), A STAT3-Decoy Oligodeoxynucleotide Suppresses Cell Growth and
Induces Apoptosis in Erlotinib Resistant SW480 Colon Cancer Cell Line by Blockage of
the STAT3 Signaling Pathway, international congress on stem cell& regenerative
medicine, 28-30 November, Tehran, Iran. (Poster presentation)
2. 1397 (2018), Induction of Mesenchymal-Epithelial Transition (MET) in HT29 Cell Line
Containing Cancer Stem-Like Cell (HT29-shE) with Differentiation Therapy Purpose for
Cancer Treatment, international congress on stem cell& regenerative medicine, 28-30
November, Tehran, Iran. (Poster presentation)
3. 1397(2018), Inhibition of T cell factor (Tcf3) transcription factor using
oligodeoxynucleotide decoy strategy increases embryonic stem cell stemness, The First
International Iranian Tissue Engineering and Regenerative Medicine Congress, 18-20
July, Tehran, Iran. (Oral presentation)
4. 1397(2018), Synthesis, characterization and in vivo evaluation of gelatin-hydroxyapatite-
silicon carbide nanocomposite scaffold seeded with mesenchymal stem cells for bone
tissue repair, The First International Iranian Tissue Engineering and Regenerative
Medicine Congress, 18-20 July, Tehran, Iran. (Poster presentation)
5. 1396 (2017), Simultaneous Targeted Inhibition of Sox2-Oct4 Transcription Factors Using
Decoy Oligodeoxynucleotides to Repress Stemness Properties in Mouse Embryonic Stem
Cells, Te 2nd National Congress of Integrative Oncology, 18-20 October, Tehran, Iran.
(Rapid Oral presentation)
6. 1396 (2017), Myc Decoy Oligodeoxynucleotide Inhibits Growth and Modulates
Differentiation of Mouse Embryonic Stem Cells as a Model of Cancer Stem Cells,
international congress on stem cell& regenerative medicine, 13-15 July, Tehran, Iran.
(Oral presentation)
7. 1396 (2017), Enrichment of Cancer Stem-Like Cells from Colorectal Cancer Cell Line
HT29 with Induction of Epithelial-Mesenchymal Transition (EMT) Using Lentiviral
Vector Carrying E-Cadherin ShRNA, 13-15 July, Tehran, Iran. (Poster presentation)
8. 1395 (2016), Osteoblast–seeded bioactive glass/gelatin nanocomposite for the repair of
an experimental model of critical-size bone defect, 3nd Iranian Congress on Progress in
Tissue Engineering and Regenerative Medicine, 28-30 Mehr, Tehran, Iran.(Oral
presentation)
9. 1395 (2016), Osteoblast–seeded bioglass/gelatin nanocomposite: a promising bone
substitute in critical-size calvarial defect repair in rat. 12th Royan International Congress
on Stem Cell Biology & Technology, Royan Institute, Tehran, Iran.(Poster
presentation)
10. 1394 (2015), Enhanced Repair of Rat Critical Size Calvarial Defect Using Osteoblast-like
and Umbilical Vein Endothelial Cells seeded in Hydroxyapatite/Gelatin Scaffolds. 2nd
Iranian Congress on Progress in Tissue Engineering and Regenerative Medicine.16-18
Aban, Tehran, Iran.(Oral presentation)
11. 1391 (2012), Investigating the Effects of Electrical Stimulation via Gold Nanoparticles
for In Vitro Nerve Regeneration. The First Iranian Annual Congress on Progress in
Tissue Engineering and Regenerative Medicine 30 ordibehesht ,Tehran, Iran.(Poster
presentation)
12. 1391 (2012), An Osteoblast-Seeded Gelatin-Hydroxyapatite Nano-Composite for the
repair of an Experimental Model of Critical-Size Bone Defect. The First Iranian Annual
Congress on Progress in Tissue Engineering and Regenerative Medicine 30 ordibehesht,
Tehran, Iran.(Poster presentation)
13. 1391 (2012), Bone Healing Effects of a Tissue-Engineered Gelatin-Hydroxyapatite
Nano-Composite Seeded with Osteoblasts and Endothelial Cells in the Management of an
Experimental Model of Critical-Size Bone Defect in Rats. The First Iranian Annual
Congress on Progress in Tissue Engineering and Regenerative Medicine 30 ordibehesht
,Tehran, Iran.(Poster presentation)
14. 1391 (2012), Enhancement of Bone Healing Properties of a Bioglass-Gelatin Composite
with Endothelial Cells. The First Iranian Annual Congress on Progress in Tissue
Engineering and Regenerative Medicine 30 ordibehesht ,Tehran, Iran.(Poster
presentation)
15. 1390 (2011), Attend in the first MBA- Biotechnology congress 23 Azar, Tehran, Iran.
16. 1392 (2013), Attend in the second MBA- Biotechnology congress 20 ordibehesht,
Tehran, Iran.
17. 1392 (2013), Attend in the first Iranian annual congress, 30 ordibehesht, Tehran, Iran.
18. 1389 (2010), Attend in the eleventh medical education congress, 10-11 ordibehesht,
Tehran, Iran.
Football, Volleyball, Traveling, Reading.
Persian (mother tongue), English (average), Arabic (not advanced).
1. Ali Samadikuchaksaraei, MD, PhD, DIC, CWC, MIRIMC, FRIPH
Consultant in Regenerative Medicine and Tissue Engineering
Consultant in General Medicine
Associate Professor (Lecturer)
Department of Biotechnology
Cellular and Molecular Research Center (CMRC)
Tehran University of Medical Sciences
PO Box: 14155/6183
Tehran, Iran
Tel: (+98 21) 8805 2984
Mobile: (+98 912) 55-77-66-9
Fax: (+98 21) 8805 4355
+1 530 380 7337 (Web-based, California)
+44 870 913 4099 (Web-based, UK)
Emails: [email protected]
2. Mehdi Kadivar, PhD of medical biotechnology, Associate Professor
Biochemistry Department, Pasteur institute of Iran
Tel: (+98 21) 669-69298
Mobile: (+98 912) 412-8860
Fax: (+98 21) 664-65132
Email: [email protected]
3. Mina Ebrahimi-Rad, PhD of medical biotechnology, Assistant Professor
Biochemistry Department, Pasteur institute of Iran
Tel: (+98 21) 641-12178
HOBBIES
LANGUAGES
REFERENCES
Mobile: (+98 912) 325-7479
Fax: (+98 21) 664-65132
Email: [email protected]
4. Yousef Mortazavi, PhD: Professor, Head of Medical Biotechnology Department,
Zanjan university of medical sciences, Zanjan, Iran.
Tel:(+98)24 33440301
E-mail: [email protected]