curriculum vitae john el-attrache, phcurriculum vitae john el-attrache, ph.d page 2 2001 research...

Curriculum Vitae John El-Attrache, Ph.D

CURRICULUM VITAE

John El-Attrache, Ph.D PRESENT POSITION AND ADDRESS: Title: Director of Scientific Services Office: 913-894-0230

Office: Ceva Animal Health Mobile: 512-239-9325 8906 Rosehill Road Lenexa, KS 66215 Email: [email protected]

Home: 10590 S. Glenview Lane Olathe, KS 66061 EDUCATION: Degree/Training Conferring Institution Field Year Bachelor of Science Texas Tech University Biochemistry 1992 Master of Science University of Georgia Med. Microbiology 1999 Doctor of Philosophy University of Georgia Med. Microbiology 2001 PROFESSIONAL EXPERIENCE AND ACADEMIC APPOINTMENTS: 1992 Research Assistant / Microbiology Assistant Texas Tech University Department of Biological Sciences 1994 Laboratory Coordinator Poultry Diagnostic and Research Center Virology Laboratory Athens, Georgia 1997 Research Coordinator I Poultry Diagnostic and Research Center Virology Laboratory Athens, Georgia

Curriculum Vitae John El-Attrache, Ph.D 2001 Research Associate

University of Georgia - Postdoctoral College of Veterinary Medicine Department of Avian Medicine Athens, Georgia

2002 Assistant Professor

Texas A&M University College of Veterinary Medicine Departments of Pathobiology/Poultry Science College Station, Texas 77843-4467

2006 Senior Manager, Business Development Life Technologies Austin, Texas 2011 Director of Scientific Services Ceva Animal Health Lenexa, Kansas

AWARDS AND HONORS: 2004 – Departmental nominee for the Montague Center for Teaching Excellence Scholar Award 2005 - Departmental nominee for the Montague Center for Teaching Excellence Scholar Award 2005 – Master Teacher Panel – Teaching Excellence Incentive Grant TEACHING EXPERIENCE: A 33 % teaching effort that encompassed all aspects of poultry health with emphasis on avian virological diseases. Undergraduate, graduate and professional students are taught in both the classroom and laboratory setting for the below listed curriculum. Undergraduate teaching includes VTPB 334 – Poultry Disease (course coordinator), POSC 201 General Avian Science (course coordinator), POSC 291 - Poultry Science Sophomore Seminars (guest lecturers) and POSC 202 Avian Science Laboratory (guest lecturer). Veterinary professional students are taught aspects of poultry health and disease in a second year clinical correlates class VMID 921 and a 3rd year scheduled VMID 985 didactic Poultry Disease elective as well as guest lectures for 1st year CVM Microbiology students and a 3rd year didactic Emerging Disease elective.

Curriculum Vitae John El-Attrache, Ph.D Undergraduate:

Credit % of Formal Contact Hr Course Title Institution Hr Course Dates Poultry Diseases TAMU-VTPB 04 66 75 Spring 03 Poultry Seminars TAMU-POSC 03 Gst. Lect. 1.5 Spring 03 Poultry Seminars TAMU-POSC 03 Gst. Lect. 1.5 Fall 03 Poultry Diseases TAMU-VTPB 04 66 75 Spring 04 Poultry Seminars TAMU-POSC 03 Gst. Lect. 1.5 Fall 04 Poultry Diseases TAMU-VTPB 04 33 22.5 Spring 05 Poultry Seminars TAMU-POSC 03 Gst. Lect. 1.5 Fall 05 Avian Science Lab TAMU-POSC 01 Gst. Lect. 4 Fall 05 General Avian Sci. TAMU-POSC 03 100 45 Fall 05 Professional:

Credit % of Formal Contact Hr Course Title Institution Hr Course Dates Poultry Didactic TAMU-VMID 04 25.0 15 Spring 03 Clin. Correlates III TAMU-VMID 02 12.5 24 Fall 03 Emerging Diseases TAMU-VMID 04 Gst. Lect. 3 Spring 04 Microbiology II VTPB 911 04 Gst. Lect. 1 Spring 04 Clin. Correlates III TAMU-VMID 02 12.5 24 Fall 04 Microbiology II VTPB 911 04 Gst. Lect. 1 Spring 05 Emerging Diseases TAMU-VMID 04 Gst. Lect. 3 Spring 05

Curriculum Vitae John El-Attrache, Ph.D Graduate:

Credit % of Formal Contact Hr Course Title Institution Hr Course Dates Avian Virus Dis. UGA 04 Gst. Lect. 4.0 Lect Fall 01-02 Avian Virus Dis. UGA 04 Instructor 15.0 Lab Fall 94-02 Special Topics TAMU-VTPB 04 100 25 Fall 04 Avian Virology TAMU-VTPB 04 33 25 Spring 05 Ad. Avian Dis. TAMU-POSC 03 100 Dist. Ed. Spring 05 Special Topics TAMU-VTPB 01 100 15 Fall 05 Student Workers: Name Dates Zachary Taylor Fall 03 Roger Rogers Spring 04 T. J. Klein Summer - Fall 05 Graduate Students:

Name Degree Institution Committee Member Dates Jaime Thurk MS TAMU/VTPB Major Advisor 5/03-5/05 Blair Telg DVM-Ph.D TAMU/VTPB Major Advisor 5/03-2009 Keith Amiess Ph.D TAMU/POSC Committee Member 1/03-5/05 Adrianna Barri MS TAMU/POSC Committee Member 1/03-12/04 Zachary Taylor MS TAMU/POSC Major Advisor 9/03-2006 Blayne Mozisek MS TAMU/POSC Major Advisor 1/04-8/05 Pam Ferro Ph.D TAMU/VTPB Major Advisor 9/04-2007 Adam Jester MS TAMU/POSC Major Advisor 9/04-2006 Blayne Mozisek DVM-Ph.D TAMU/VTPB Major Advisor 9/05-2012

Curriculum Vitae John El-Attrache, Ph.D Predoctoral Fellows: Name Institution Dates Blair Telg TAMU-Summer Vet. Student Research Fellow Summer 03 Melanie Warthberg TAMU-Summer Vet. Student Research Fellow Summer 04 Postdoctoral Fellows: Name Institution Dates Katarina Tosic Borlaug Young Scientist Fellow Aug-Oct 04 RESEARCH/SCHOLARLY ACTIVITIES: A 33% research effort which is primarily focused on poultry diseases. More specifically, a research program that is applied in the development of better diagnostics, vaccines and viral therapeutics for the major respiratory diseases that affect the commercial poultry industry. Emphasis of research has recently been placed on avian influenza.

DATE INVESTIGATORS TITLE SOURCE AMOUNT

July 1995-June 1996

Villegas, P. J. El-Attrache and H. Sellers

Isolation, identification and control of avian viruses

Vet.Med. Ag. Research

$26,000.

1996-97 Villegas, P. (J. El-Attrache)

The effect of Sarafloxacin on the herpesvirus of turkeys vaccine

Abbott Labs $3,000.

July 1996-June 1997



Vet. Med. Ag. Research

$30,000.

July 1997-June 1998




$24,000.

1998 Villegas, P. (J. El-Attrache)

Studies on Marek’s Disease Pfizer, Inc. $13,014.






July 1998-June 1999


Methods for isolation, identification and control of avian viruses


$67,500.

Oct. 1998-Sept. 2000

García, M. J. El-Attrache and P. Villegas

Development and application of an RT-PCR assay for the detection of

avian leukosis virus subgroup J (ALV-J) from

meat-type chickens

U.S. Poultry and Egg

Association $55,198.


Further Studies on Marek’s Disease

Pfizer, Inc. $49,883.



Pfizer, Inc. $49,370.



Pfizer, Inc. $36,364

July 1999-June 2000

Villegas, P. and J. El-Attrache



$65,400.

July 2000-June 2001


Advancements in the isolation, characterization and control of avian

viruses


$65,400.

April 2001- April 2002

Villegas, P. (J. El-Attrache)

Adaptation of IBV strains to the intestinal tract

Merial - Select

$75,565.

July 2001- June 2002


Advancements in the isolation, characterization and control of avian

viruses


$54,282.

Nov. 2001-March 2003

Villegas, P., A. Banda and J. El-Attrache

Molecular and pathogenic analysis of coinfection with IBDV and CAV

USPEA $20,000

March 2002-March 2004


Pathological and serological assessment of a live avian adenovirus

vaccine Aviagen $39,628

July 2002- June 2003




$64,500

TEXAS A&M UNIVERSITY

COLLEGE OF VETERINARY MEDICINE DEPARTMENT OF PATHOBIOLOGY

Nov. 2002-Sep. 2004

El-Attrache, J Research and laboratory establishment TAMU/CVM

Veterinary Pathobiology

$100,000

Summer 2003 El-Attrache, J. and B. Telg

Determination and analysis of exotic Newcastle Disease virus (ENDV) shedding in previously vaccinated

chickens

Summer Veterinary

Student Research Fellows Program

$3,500


April 2003 – April 2005

El-Attrache, J., D. Caldwell and I. Tizard

Analysis of CpG-motif oligonucleotides as potential poultry immmunostimulatory agents

USDA - Formula Animal

Health (1433)

$45,550

July 2003-July 2004

El-Attrache, J., S. Naqi, P. Villegas, J. Linares and B. Lupiani

Development of a sensitive and serotype specific lateral-flow

immunoassay for the detection of infectious bronchitis virus

USPEA $56,000

Summer 2004 El-Attrache, J. and M. Warthberg

Detection and analysis of Infectious Laryngotracheitis (ILT) Virus field

isolates in domestic poultry from Laser Capture Microdissection (LCM)

tissues.

Summer Veterinary

Student Research Fellows Program

$6,000

Fall 2004- 2007

Lupiani, B, J. El-Attrache, and S. Reddy

Center for Foreign and Zoonotic Diseases Defense. Avian Influenza

TAMU

Department of Homeland Security

$331,000

Feb. 2005-Sept 2009

J. El-Attrache H-3023 Control of Emerging & Re-

emerging Poultry Respiratory Diseases in the United States

USDA-CSREES

Multistate (NC-1019)

Fall 2005- 2007

Lupiani B./El-Attrache J./Reddy S/Berghman

Integrated Project for the Prevention and Control of Avian Influenza in the

U.S. USDA-NRI $159,485

July 2005-July 2007

J. El-Attrache Generation of an avian adeno-

associated virus vector system to control avian influenza

USDA - Formula Animal Health

$48,750

August 2005 J. El-Attrache

Increasing and enhancing learning opportunities for graduate and professional students through interactive digital multimedia

Master Teaching

Panel $2,500

BIBLIOGRAPHY:

Publications in Refereed Journals: Ruano, M., J. El-Attrache, and P. Villegas. A rapid plate hemagglutination assay for the identification

of Infectious Bronchitis Virus. Avian Diseases. 44:99-104. 2000. Garcia, M. C., J. El-Attrache, N. Ikuta, V. R. Lunge, A. S. K. Fonseca, S. M. Riblet and P. Villegas.

Current advances in the diagnosis of avian leukosis virus by the polymerase chain reaction. Proceedings: International Symposium on ALV-J and other Avian Retroviruses. World Veterinary Poultry Association. Rauischholzhausen, Germany. June 5-8, 2000.

Curriculum Vitae John El-Attrache, Ph.D Sellers, H.S., P.Villegas, J. El-Attrache, D. Kapczynski, and Corrie C. Brown. Detection of Infectious

Bursal Disease Virus in experimentally infected chickens using in situ hybridization. Avian Diseases. 45:26-33. 2001.

Ikuta, N., J. El-Attrache, P. Villegas, M. García, V.R. Lunge, A.S.K. Fonseca, C. Oliveira, and E.K.

Marques. Molecular characterization of Brazilian infectious bursal disease viruses. Avian Diseases. 45:297-306. 2001.

El-Attrache, J., P. Villegas, B. O’Connor, J.R. Buhr, and G.N. Rowland. Adenovirus pathogenicity in

immature ostriches. Avian Diseases. 45:442-446. 2001. Ragland, W.L., R. Novak, J. El-Attrache and K. Ester. Interference with transcription for interferon by

CIAV. Proceedings: International Symposium on Infectious Bursal Disease and Chicken Infectious Anemia. World Veterinary Poultry Association. Rauischholzhausen, Germany. June 16-20, 2001.

Ruano, M., J. El-Attrache and Pedro Villegas. Efficacy comparisons of disinfectants used by the

commercial poultry industry. Avian Diseases. 45:972-977. 2001. El-Attrache, J. and P. Villegas. Genomic identification and characterization of avian adenoviruses

associated with inclusion body hepatitis. Avian Diseases. 45:780-787. 2001. Banda, A., P. Villegas, J. El-Attrache, and C. Estevez. Molecular characterization of seven field

isolates of infectious bursal disease virus obtained from commercial broiler chickens. Avian Diseases. 45:620-630. 2001.

Ragland, W.L., R. Novak, J. El-Attrache and K. Ester. Interference with transcription for interferon by

CIAV. Chicken anemia virus and infectious bursal disease virus interfere with transcription of chicken IFN-alpha and IFN-gamma mRNA. J Interferon Cytokine Res. Apr;22(4):437-41. 2002.

Majó, N., J. El-Attrache, P. Villegas, A. Ramis, A. Pagès, and N. Ikuta. Molecular characterization

and identification of Spanish Infectious Bursal Disease Virus field isolates. Avian Diseases. 46:859-868. 2002.

Zavala G, Dufour-Zavala L, Villegas P, El-Attrache J, Hilt DA, Jackwood MW. Lack of interaction

between avian leukosis virus subgroup J and fowl adenovirus (FAV) in FAV-antibody-positive chickens. Avian Diseases. 46(4):979-84. 2002.

Garcia, Maricarmen, John El-Attrache, Sylva Riblet, Nilo Ikuta, Vagner R. Lunge, Andre S. K.

Fonseca and P. Villegas. Development and application of a reverse transcriptase (RT) nested polymerase chain reaction (PCR) test for the detection of exogenous avian leukosis virus. Avian Diseases. 47:41-53. 2003.

Banda, A., P. Villegas and J. El-Attrache. Molecular characterization of international infectious bursal

disease virus field isolates from the United States and Latin America. Avian Diseases. 47:87-95.


2003. Estevez, C., P. Villegas, and J. El-Attrache. A recombination event, induced in ovo, between a low

passage infectious bronchitis virus filed isolate and a highly embryo adapted vaccine strain. Avian Diseases. 47:1282-1290. 2003.

Alvarado, I., P. Villegas, J. El-Attrache, and T.P. Brown. Evaluation of the protection conferred by

commercial vaccines against the California 99 isolate of infectious bronchitis virus. Avian Diseases. 47: 1298-1304. 2003.

Banda, A., P. Villegas and J. El-Attrache. Heteroduplex mobility assay for genotyping infectious

bursal disease virus. 48: 851-862. 2004. Ameiss, K.A., J. El-Attrache, A. Barri, A.P. McElroy and D.J. Caldwell. Efficacy of Oral CpG-ODNs

as Mucosal Adjuvants when Co-Administered with Protein Antigen in Chickens. Submitted. Developmental and Comparative Immunology. 2005.

Ameiss, K.A., J. El-Attrache, and D.J. Caldwell. Efficacy of aral CpG-ODNS as mucosal adjuvants when co-administered with protein antigen in chickens. To be submitted. Poultry Science. 2005 El-Attrache, J., P. Villegas, A. Banda and M.C. García. Comparative analysis of the EV-O-derived cell

line DF-1 and line-0, C/E chicken embryo fibroblast for the isolation and detection of subgroup J avian leukosis virus. Avian Diseases. To be submitted. 2005.

El-Attrache, J., P. Villegas, S. Callison and A. Banda. The design and evaluation of morpholinos as

potential inhibitors of avian leukosis virus replication. Poultry Science. To be submitted. 2005. El-Attrache, J. and P. Villegas. Molecular and classical analysis of nested RT-PCR ALV-J positive

plasma samples undetected by virus isolation. Avian Diseases. To be submitted. 2005. Mozisek, B. and J. El-Attrache. Development of a quantitative real-time RT-PCR for rapid detection and quantification of exogenous avian leukosis virus. To be submitted. Journal of Virological Methods. 2005. Telg, B.E., J. Thurk, L. Berghman and J. El-Attrache. Enhancement of Newcastle Disease detection in diagnostic samples utilizing immunomagnetic beads. Avian Diseases. Manuscript in Preparation. Mozisek, B., J. El-Attrache, Q. Hoang, R.C. Willis, W. Xu, M.A. Bounpheng, and X. Fang. High Throughput Viral RNA isolation and quantitative real-time RT-PCR assay for detection of exogenous Avian Leukosis Virus. Journal of Veterinary Diagnostics. Manuscript in Preparation.

Curriculum Vitae John El-Attrache, Ph.D Refereed Proceedings/Published Abstracts: El-Attrache, J., and P. Villegas. Principle effects of variable antigen concentration on Newcastle

Disease hemagglutination inhibition titers. Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract S173). January 20-21, 1997.

El-Attrache, J., and P. Villegas. Genomic characterization and identification of recent pathogenic avian

adenovirus field isolates. Proceedings of the 134th Annual Meeting of the American Veterinary Medical Assn., Reno, NV. (Poster PP40, p. 158). July 19-24, 1997.

Gómez, Luis B., Jr., P. Villegas, and J. El-Attrache. Effect of antibiotics on the herpesvirus of turkeys

vaccine. Proceedings of the 134th Annual Meeting of the American Veterinary Medical Assn., Reno, NV. (Abstract p. 182). July 19-24, 1997.

Villegas, P., M. Ruano, and J. El-Attrache. Attenuation, stability, and protection afforded by infectious

laryngotracheitis virus. Proceedings of the 134th Annual Meeting of the American Veterinary Medical Assn., Reno, NV. (Abstract p. 180). July 19-24, 1997.

El-Attrache, J., and P. Villegas. Characterization of avian adenoviruses associated with inclusion body

hepatitis. Proceedings of the 39th annual meeting of the Southern Conference on Avian Diseases, Atlanta, GA. (Abstract no. 101). January 19-20, 1998.

Ruano, M., J. El-Attrache, and P. Villegas. Development of a rapid plate hemagglutination assay for

identification of infectious bronchitis. Proceedings of the 39th annual meeting of the Southern Conference on Avian Diseases, Atlanta, GA. (Abstract no. 97). January 19-20, 1998.

El-Attrache, J., N. Ikuta, P. Villegas, and M. Ruano. Molecular characterization and identification of

Infectious Bursal Disease Virus field isolates. Proc. of the 135th American Veterinary Medical Assn. annual convention, Baltimore, MD. (Abstract p. 180). July 25-29, 1998.

Ruano, M., P. Villegas, and J. El-Attrache. Correlation of a neuraminidase-treated hemagglutination

assay with the reverse transcriptase polymerase chain reaction test (RT-PCR) for infectious bronchitis virus. Proc. of the 135th American Veterinary Medical Assn. annual convention, Baltimore, MD. (Abstract p. 182). July 25-29, 1998.

Zavala, G., J. El-Attrache, P. Villegas, and M. Ruano. Effects of subgroup J avian leukosis virus

infection on broilers and young broiler breeders challenged with virulent Marek’s disease virus. Proc. of the 135th American Veterinary Medical Assn. annual convention, Baltimore, MD. (Abstract p. 183). July 25-29, 1998.

El-Attrache, J., and P. Villegas. Characterization of avian adenoviruses associated with inclusion body

hepatitis. Proceedings of the 1998 Poultry Science Assn. annual meeting, Pennsylvania State University, University Park, PA. (Abstract no. S101, p. 134). August 2-5, 1998.

Curriculum Vitae John El-Attrache, Ph.D Ruano, Miguel, J. El-Attrache, and P. Villegas. Development of a rapid plate hemagglutination assay

for identification of infectious bronchitis. Proceedings of the 1998 Poultry Science Assn. annual meeting, Pennsylvania State University, University Park, PA. (Abstract no. S97, p. 133). August 2-5, 1998.

El-Attrache, J., M. García, P. Villegas, and Sylva Riblet. Sequence analysis comparisons of the

subgroup J avian leukosis virus unique envelope (env) gene. 136th annual convention of the American Veterinary Medical Assn., New Orleans, LA. JAVMA 215(11): 1677. July 12, 1999.

Sellers, H., P. Villegas, J. El-Attrache, D. Kapczynski, and C. Brown. Detection of infectious bursal

disease virus in experimentally infected chickens using in situ hybridization with a riboprobe. Proc. of the 136th American Veterinary Medical Assn. annual convention, New Orleans, LA. JAVMA 215(11): 1684. July 13, 1999.

Dufour-Zavala, L., L. Teodoro de Barros, J. Vaillancourt, R. Resurreccion, P. Villegas, and J. El-

Attrache. Investigation of an inclusion body hepatitis outbreak in Northeast Georgia. Proc. of the 136th American Veterinary Medical Assn. annual convention, New Orleans, LA. July 14, 1999.

Zavala, G., L. Dufour-Zavala, P. Villegas, R. Resurreccion, and J. El-Attrache. Co-infection in meat

type chickens and specific pathogen free chickens with subgroup J avian leukosis virus and group E fowl adenovirus obtained from clinical cases of inclusion body hepatitis. 136th annual convention of the American Veterinary Medical Assn., New Orleans, LA. JAVMA 215(11): 1689. July 12, 1999.

Villegas, P., J. El-Attrache, A. Banda, G. N. Rowland, and J. Glisson. Effect of infectious bursal

disease variant-like viruses on broiler flocks. 136th annual convention of the American Veterinary Medical Assn., New Orleans, LA. JAVMA 215(11): 1687. July 13, 1999.

Sellers, H.S., P. Villegas, J. El-Attrache, D.R. Kapczynski, and C.C. Brown. 1999. Detection of

infectious bursal disease virus in experimentally infected chickens using in situ hybridization with a riboprobe. American Veterinary Medical Association/American Association of Avian Pathologists Annual Meeting. New Orleans, Louisiana.

Sellers, H.S., P. Villegas, J. El-Attrache, D.R. Kapczynski, and C.C. Brown. 1999. Pathogenesis of

infectious bursal disease virus pathotypes assessed by in situ hybridization. American Society for Virology. 18th Annual Meeting. Amherst, Massachusetts.

El-Attrache, J., M. C. Garcia, N. Ikuta, V. R. Lunge, A. S. K. Fonseca, S. M. Riblet and P. Villegas.

Current advances in the diagnosis of avian leukosis virus by the polymerase chain reaction. International Symposium on ALV-J and other Avian Retroviruses. Rauischholzhausen, Germany. June 5-8, 2000.

Estévez, C. N., P. Villegas, and J. El-Attrache. Characterization of a field isolate of infectious


bronchitis virus in layers by classical virological methods. International Poultry Scientific Forum Abstracts, Atlanta, Georgia. Abst. No. 170, pp. 37. Jan. 17-18, 2000.

Banda, A., J. El-Attrache, M. Ruano, and P. Villegas. Molecular Characterization of infectious bursal disease virus associated with respiratory problems and poor performance on broilers farms in Georgia, Atlanta, Georgia. Abst. No. 174, pp. 38. Jan. 17-18, 2000.

Fonseca, A.S.K., Garcia, M., Ikuta, N., Lunge, V.R., El-Attrache, J. and Marques, E. Detecção e

diferenciação molecular de subgrupos exógenos do vírus da leucose aviária - alv.". K. Conferência APINCO de Ciência e Tecnologia Avícolas 2000. Campinas, SP, Brasil. Maio, 2000.

Banda, Alejandro, J. El-Attrache, P. Villegas, M. Ruano, and C. Estevez. Clinical and molecular

characterization of infectious bursal disease virus field strains obtained from commercial broilers. Proc. of the 137th Annual Meeting of the American Veterinary Medical Association, Salt Lake City, UT. Abstract p. 672. July 22-26, 2000.

El-Attrache, J., P. Villegas, and M. García, M. Comparative analysis of the EV-O-derived cell line DF-

1 and line-0, C/E chicken embryo fibroblast for the isolation and detection of subgroup J avian leukosis virus. Proc. of the 137th Annual Meeting of the American Veterinary Medical Association, Salt Lake City, UT. Abstract p. 716. July 22-26, 2000.

Estevez, C., J. El-Attrache, and P. Villegas. In-ovo recombination of the S1 coding region of an

infectious bronchitis virus field isolate. Proc. of the 137thAnnual Meeting of the American Veterinary Medical Association, Salt Lake City, UT. Abstract p. 682. July 22-26, 2000.

Ruano, M., J. El-Attrache, and P. Villegas. Pathogenicity studies of four serotype-1 Marek’s disease

virus field isolates. Proc. of the 137th Annual Meeting of the American Veterinary Medical Association, Salt Lake City, UT. Abstract p. 723. July 22-26, 2000.

Majó, N., J. El-Attrache, P. Villegas, A. Ramis, A. Pagès, and N. Ikuta. Caracterización molecular de nueve cepas de campo españolas de virus de la enfermedad de Gumboro. Proc. of Congreso Internacional de producción y Sanidad Animal, EXPOAVIGA, Barcelona, Spain. pp. 161-166. November 7-10, 2000.

Banda, A., J. El-Attrache and P. Villegas. Isolation and identification of a US IBDV isolate with a

vvIBDV molecular marker. Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract No. 184, p. 40). January 15-16, 2001.

Ruano, M., J. El-Attrache and P.Villegas. Increasing the spectrum of the hemagglutination inhibition

(HI) test for infectious bronchitis virus antibody detection. Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract No. 180, p. 40). January 15-16, 2001.

Banda, A., P. Villegas, and J. El-Attrache. Detección y tipificación de cepas virus de la enfermedad

infecciosa de la bolsa mediante técnicas moleculares. Trabajo presentado ante la XXVI Reunión


Nacional de Especialistas en Ciencias Avícolas (ANECA). Acapulco, Gro. Realizada del 25 al 28 de Abril 2001.

Ragland, W.L., R. Novak, J. El-Attrache and K. Ester. Interference with transcription for interferon

by CIAV. International Symposium on Infectious Bursal Disease and Chicken Infectious Anemia. Rauischholzhausen, Germany. June 16-20, 2001.

El-Attrache, J., P. Villegas, M.C. Garcia. Classical and Molecular Analysis of nested RT-PCR ALV-J

positive plasma samples undetected by virus isolation coupled with antigen capture ELISA. 2001. American Association of Veterinary Medicine / American Association of Avian Pathologists. 138th Meeting. Boston, MA.

Alvarado, I., P. Villegas, J. El-Attrache and J. Glisson. Protection Afforded by Commercial Vaccines

Against the Nebraska 95 IBV Field Isolate. 2001. American Association of Veterinary Medicine / American Association of Avian Pathologists. 138th Meeting. Boston, MA.

Banda, A., P. Villegas, J. El-Attrache, and C. Brown. Pathogenicity of recent IBDV isolates inoculated

in commercial broilers using in situ hybridization. 2001. American Association of Veterinary Medicine / American Association of Avian Pathologists. 138th Meeting. Boston, MA.

Villegas, P., M. Ruano, J. El-Attrache, and N. Ferguson. Virological and Serological Identification of

Infectious Bronchitis Virus (IBV) isolates known as DE-072 Variants. 2001. American Association of Veterinary Medicine / American Association of Avian Pathologists. 138th Meeting. Boston, MA.

Alvarado, I., P. Villegas, J. El-Attrache, and M. Jackwood. Persistence of Infectious Bursal Disease

(IBV) Arkansas and Massachusetts vaccines in tissues from broilers. Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract No. 225, p. 51). January 14-15, 2002. (Received The C. S. Eidson Award for BEST ORAL PRESENTATION.)

Banda, A., P. Villegas, J. El-Attrache, and H. Sellers. The use of the heteroduplex mobility analysis

(HMA) for genotyping field isolates of the Infectious Bursal Disease Virus (IBDV). Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract No. 229, pp. 51-52). January 14-15, 2002.

Estevez, C.N., J. El-Attrache and P. Villegas. Sequence analysis of the avian adeno associated virus.

Proc. of Southern Conference on Avian Diseases, World Congress Center, Atlanta, GA. (Abstract No. 241, p. 54). January 14-15, 2002. (Received The C. S. Eidson Award for BEST POSTER.)

El-Attrache, J., and P. Villegas. Update on ascitis-hydropericardium syndrome. Proc. of the X

Seminario Internacional de Patología Producción. Georgia Center for Continuing Education, University of Georgia, Athens, GA. May 27-31, 2002.

Banda, Alejandro, P. Villegas, J. El-Attrache, and C. Brown. Clinical and molecular characterization

of chicken anemia virus isolates obtained from commercial broilers. Proc. of the 139th Annual


Meeting of the American Veterinary Medical Association, Nashville, TN. (Abstract p. 9). July 14-17, 2002.

El-Attrache, J., P. Villegas, S. Callison, and A. Banda. The design and evaluation of antisense

oligomers as potential inhibitors of avian leukosis virus replication. Proc. of the 139th Annual Meeting of the American Veterinary Medical Association, Nashville, TN. (Abstract p. 21). July 14-17, 2002.

Estevez, C., P. Villegas, and J. El-Attrache. Use of a human cell line to induce helper virus-free

replication of an avian adeno-associated virus. Proc. of the 139th Annual Meeting of the American Veterinary Medical Association, Nashville, TN. (Abstract p. 25). July 14-17, 2002.

Banda, A., P. Villegas, J. El-Attrache and N. Ledesma. The use of the single-strand conformational

polymorphism analysis (SSCP) for genotyping isolates of the infectious bursal disease virus (IBDV). International Poultry Scientific Program. Southern Conference on Avian Diseases. Atlanta, GA. January 20-21, 2003. (Received The C. S. Eidson Award for BEST ORAL PRESENTATION.)

El-Attrache, J., P. Villegas, G. Rosales and E. Jensen. Progeny challenge studies assessing the

protection afforded against inclusion body hepatitis field isolates by an inactivated avian adenovirus vaccine. Proc. of the 140th Annual Meeting of the American Veterinary Medical Association – XIII Congress of the World Veterinary Poultry Association. Denver, CO. July 19-23, 2003.

Villegas, P., J. El-Attrache, G. Rosales and E. Jensen. Inclusion body hepatitis: Efficacy of adenovirus

vaccines to induce antibody response in chickens. Proc. of the 140th Annual Meeting of the American Veterinary Medical Association – XIII Congress of the World Veterinary Poultry Association. Denver, CO. July 19-23, 2003.

Banda, A., P. Villegas and J. El-Attrache. Molecular characterization of field strains of infectious

bursal disease virus in the United States and some Latin American countries. Proc. of the 140th Annual Meeting of the American Veterinary Medical Association – XIII Congress of the World Veterinary Poultry Association. Denver, CO. July 19-23, 2003.

Telg, B., J. Thurk and J. El-Attrache. Determination and analysis of Newcastle Disease Virus shed in

chickens. Merck-Merial Summer Veterinary Research Scholars Program. Kansas State University – Manhattan, KS. August 1-3, 2003.

Barri, A., K. Ameiss, J. El-Attrache and D.J. Caldwell. Utilization of CpG oligodeoxynucleotides to

improve Newcastle Disease vaccination. Southern Conference on Avian Diseases. International Poultry Scientific Forum. January 26-27. Atlanta, GA. 2004

Ameiss, K., J. El-Attrache, N. Brewer, A.P. McElroy and D.J. Caldwell. Enhancement of the humoral

response in chickens via oral administration of CpG-ODNs. Southern Poultry Science Society. International Poultry Scientific Forum. January 26-27. Atlanta, GA. 2004. (Recipient of the


Poultry Science Society Outstanding Graduate Student Research Presentation Award: Pathology Section 2004)

Barri, A., J. El Attrache, K.A. Ameiss, and D.J. Caldwell. 2004. Improved Vaccination Against

Newcastle Disease Virus in Chickens: Use of CpG Motifs as Mucosal Adjuvants. Proceedings XXII World’s Poultry Congress.

Ameiss, K.A., J. El-Attrache, A. Barri, A.P. McElroy, and D.J. Caldwell. 2004. Enhancement of Homologous Vaccination to Eimeria acervulina via CpG-ODNs. Poult. Sci. 83: Suppl. 1. p.108. Telg, B., J. Thurk and J. El-Attrache. Quantitative real-time RT-PCR analysis of Newcastle Disease

Virus shed in chickens. Southern Conference on Avian Diseases. International Poultry Scientific Forum. January 26-27. Atlanta, GA. 2004. (Received The C. S. Eidson Award for BEST ORAL PRESENTATION.)

Barri, A., K. Ameiss, J. El-Attrache, A. McElroy, D. Caldwell. Effects of cytosine-

phosphate-guanosine oligodeoxynucleotides (CpG-ODN) on Salmonella vaccination or BSA immunization of neonatal chickens. Poult. Sci. January 2005.

Mozisek, B. and J. El-Attrache. Development of a high-throughput quantitative real-time RT- PCR forrapid detection of exogenous avian leukosis viruses. International Poultry Scientific Forum: SCAD/SPSS Atlanta, Georgia January 24-28, 2005. (Received The C. S. Eidson Award for BEST ORAL PRESENTATION.)

El-Attrache, J., B. Lupiani, B. Mozisek, C. Cardona, J. Li, and S. Reddy. Molecular Characterization of Recent Avian Influenza Viruses Isolated in the United States. Department of Homeland Security. Working Together: Research & Development Partnerships in Homeland Security. Boston, Massachusetts. April 27-28, 2005. El-Attrache, J. and J. Thurk. Development of a Lateral-flow immunoassay for the detection of IBV antigen. Proc. of the 142st Annual Meeting of the American Veterinary Medical Association – American Association of Avian Pathologists. Minneapolis, MN. July 16-20, 2005. Non-Refereed Publications: El-Attrache, J., G. Rowland, P. Villegas, B. O’Connor, R. Buhr. Adenovirus pathogenicity in immature ostriches. American Ostrich Research Foundation. Research Issue. April 1997. El-Attrache, J. Outbreak of poultry disease in California won’t affect holiday dinners in Texas. AgNews – News and Public Affairs – Texas A&M University Agriculture Program. November 20,

Curriculum Vitae John El-Attrache, Ph.D 2002. Exotic Newcastle Disease Confirmed in Texas Poultry Flock. Texas Cooperative Extension. Veterinary Quarterly Review. Volume 19, Number 1: 6. January-March 2003. El-Attrache, J. Bio-invaders: Safeguarding Texas aginst emerging exotic threats. Exotic Newcastle Disease. Texas A&M University “Lifescapes”. Summer 3(2): 10-12. 2003. El-Attrache, J. Exotic Newcastle Disease. CVM Today. Summer 5(1) 11. 2003. El-Attrache, J. Common poultry diseases. Popular Farming. Fall 2006. SERVICE ACTIVITIES: Service: A 33% effort focusing on improving communication between Texas A&M University – College of Veterinary Medicine researchers and the State of Texas poultry industry while also providing the industry with selected classical and molecular virology diagnostics, analysis, updated knowledge and expertise of poultry disease and health issues. Industry: Pilgrim’s Pride (Pittsburgh, TX) – Bob Hendrix (Executive Vice President)

Discussion and presentation of research opportunities that exists between Texas A&M University – College of Veterinary Medicine – Poultry Science and Texas Veterinary Medical Diagnostic Laboratory (2003)

Tyson Foods (Carthage, TX Complex) – Steve Maurer (Live Production Manager) Discussion and presentation of research opportunities that exists between Texas A&M University – College of Veterinary Medicine – Poultry Science and Texas Veterinary Medical Diagnostic Laboratory (2003)

Feathercrest Farms (Kurten, TX) – Byron Shinker (Farm Manager) Colibaccillosis (2003) Holmes Foods (Gonzales, TX) Newcastle Disease (2003) Perdue Farms Inc. (Salisbury, MD) - Miguel Ruano, DVM, MAM (Field Vet. and Lab. Manager) Assessed existing eradication program for avian leukosis virus (2004-2005)

Curriculum Vitae John El-Attrache, Ph.D Ambion Inc. (Austin, TX) – Rod Klassy, Ph.D (Business Manager – Product Development)

Avian influenza, Newcastle Disease and Avian Leukosis Virus Diagnostic assay development for the poultry industry (Nov. 2002 - present)

Elanco Animal Health – Eric Willinghan, DVM, MAM (Technical Veterinarian) Assist in isolation, confirmation and genotyping of IBV isolates (January 2005 – present)

Sanderson Farms (Bryan, TX and Laurel, MS) – Terry Thompson (Live Production Manager) Phil Stayer, DVM, MS, ACPV (Corporate Veterinarian) Assist in isolation, confirmation and genotyping of IBV and avian adenoviruses (March 2004 – present).

Texas Poultry Federation: Board Meeting Huntsville, TX April 10-11, 2003 Commercial Egg Clinic College Station, TX April 22-23, 2003 Texas Broiler Council Nacogdoches, TX June 18, 2003 TPF Convention-Board Meeting San Antonio, TX July 24-26, 2003 TPF – Texas Broiler Symposium and Poultry Processors’ Workshop Nacogdoches, TX September 30-October 1, 2003 Commercial Egg Clinic College Station, TX April 15-16, 2004 TPF Convention-Board Meeting San Antonio, TX

Curriculum Vitae John El-Attrache, Ph.D July 22-24, 2004 Commercial Egg Clinic College Station, TX April 12-13, 2005 TPF Convention-Board Meeting San Antonio, TX July 21-23, 2005 TPF – Texas Broiler Symposium and Poultry Processors’ Workshop Nacogdoches, TX October 19-20, 2005 Texas Veterinary Medical Diagnostic Laboratory: Jose Linares, DVM, ACPV Resident Director (Gonzales, TX) Assist in laboratory confirmation of avian viral diseases. 2002-2005. Lelve Gayle, DVM (Executive Director) Chair Emergency Reserve Force for the College of Veterinary Medicine Texas Animal Health Commission: Max Coats, DVM, MS Deputy Director Assisted in avian influenza eradication process in Gonzales, TX. April 2004.

Laboratory confirmation and characterization of infectious laryngotracheitis isolate in East Texas. June 2004

Texas A&M University

Poultry Institute for Youth – Participate in activities and introduce poultry diseases to future recruits to TAMU.

June 24, 2003 June 28, 2005

Foreign Animal Zoonotic Disease Center – Provide Executive Committee and Senior Staff with technical assistance and public address in regards to avian influenza.

Fall 2004 – present. International Agriculture Program – Mike McWhorter, Ph.D (Inter Training Coordinator). June 3, 2003 – Poultry production, processing and food safety (India Dignitaries)

Curriculum Vitae John El-Attrache, Ph.D Aug 1 – Oct 4, 2004 - Borlaug Fellowship Program Host April 5 – 8, 2005 - Borlaug Fellowship Program Serbia/Montenegro visit September 22, 2005 - Meat and Poultry Inspection Procedures and Issues (North Africa Dignitaries) Texas A&M College of Medicine, Department of Humanities in Medicine – Facilitator and participant of the Influenza Tabletop Exercise for Second Year Medical Students and Veterinary Students. April 21, 2005. Professional Organizations and Service: American Association of Avian Pathologists Texas Poultry Federation Intercollegiate Faculty of Virology Major Committee Assignments: Departmental: Department of Poultry Science

2003 Farm Space Utilization 2003-04 Co-Chair of Seminar Organization Committee

2004-05 Poultry Genomics Search Committee Department of Veterinary Pathobiology

2003-05 Biodefense and Emerging Infectious Disease Search Committee

College: College of Veterinary Medicine

2004-05 Departmental Head Substitute for Executive Committee Meetings 2005 CVM Interview Committee (Faculty Member) 2005-08 Student Faculty Relations – Student Honor Code Council

Foreign Animal Zoonotic Disease Center 2005-08 Internal Advisory Committee Professional:

AMEVEA – Asociación Colombiana de Médicos Veterinarios y Zootecnistas Especialistas en Avicultura


Organization Committee 1994 VIII Seminario Internacional de Patología y Producción Aviar

1998 IX Seminario Internacional de Patología y Producción Aviar 2002 X Seminario Internacional de Patología y Producción Aviar SCAD – Southern Conference of Avian Diseases

2003-2004 Director 2004-2005 President

AAAP – American Association of Avian Pathologists 2004-2006 Respiratory Disease Committee 2004-2007 Biologics Committee

OTHER SCHOLARLY ACTIVITY: Conferences – Symposia Attended: Grantwriting Seminar for Research Investigators – Office of Proposal Development - OVPR September 24-25, 2003 Organized Workshops/Seminars/Meetings: Poultry Health Meeting – Hosted by the Department of Poultry Science discussing initiatives to be pursued by USDA-ARS, TVMDL, TAHC, VTPB and POSC. January 11, 2005. SCAD - Southern Conference of Avian Diseases – Approximately 300 Attendees - January 24-25, 2005.

Coccidia Workshop and Clinic – TAMU/VTPB/POSC, Elanco Animal Health and Sanderson Farms Live Production Unit - Approximately 35 attendees - June 23, 2005. Texas Gamebird Conference – TAMU/VTPB/POSC/TAES/USDA – Approximately 100 attendees - August 5-6, 2005. Poultry Health Group Meeting at the Texas Broiler Symposium – TVMDL-VTPB-POSC and Corporate Veterinarians and live production personnel discussing Texas poultry health issues. Nacogdoches, Texas. October 20, 2005.

Curriculum Vitae John El-Attrache, Ph.D Invited Presentations Update on ascitis-hydropericardium syndrome. Proc. of the X Seminario Internacional de Patología Producción. Georgia Center for Continuing Education, University of Georgia, Athens, GA. May 27-31, 2002. Disease management and bio-security practices in poultry production systems. Poultry Production, Processing and Food Safety Practices. US Grains Council. Washington, DC – College Station, TX. May 28-June14, 2003. Do chickens get sick? Poultry Institute for Youth. Texas A&M University. Department of Poultry Science. June 24, 2003. Future antibiotic usage in the Poultry Industry. Texas Broiler Symposium. Nacogdoches, TX. September 30 – October 2, 2003. High Economic Impact Diseases in the USA. Poultry Meat Industry in Mexico and the USA. Texas A&M University Center in Mexico City and The College of Veterinary Medicine at the UNAM. Texas A&M University Center in Mexico City located on Reforma Av. 76 15th floor, Col. Juarez on November 25, 2003. Molecular Aspects of Avian Influenza. Texas Poultry Federation. Executive meeting. Kerrville, TX. April 29- May 1, 2004.

Respiratory Diseases in Poultry: Sources and prevention. Association of Veterinarians Specialized in Poultry Science - Ecuador (AMEVEA-E). Quito, Ecuador. August 9, 2004. Infectious bronchitis: The virus and strain variation, methods to detect and differentiate these variants. Association of Veterinarians Specialized in Poultry Science - Ecuador (AMEVEA-E). Quito, Ecuador. August 9, 2004. Overview of poultry diagnostics. Ambion. Austin, Texas. September 23, 2004. Avian influenza: Do we need to press the panic button? SAVMA, College Station, Texas. March 12, 2005 Avian influenza – agricultural and human health. Borlaug Fellowship Young Scientists Program. Serbia – Montenegro. April 6-8, 2005. Avian influenza: Time to press the panic button? Texas Commercial Egg Clinic. Bryan, Texas. April 12-13, 2005 Update on Avian Influenza. Shelby County Texas Agricultural Committee. Center, Texas. August 25, 2005.

Curriculum Vitae John El-Attrache, Ph.D A status review for avian influenza. A Training Program in Meat and Poultry Inspection Procedures and Issues for North African Food Safety Professionals. Texas A&M University – College of Veterinary Medicine. September 22, 2005. TVMDL-VTPB-POSC Poultry Health Group. Texas Broiler Symposium. Nacogdoches, Texas. October 20, 2005. Avian Influenza. Shelby County Rotary Club. Center, Texas. November 1, 2005. Professional Consultations Pfizer Inc. – 1999 Avian virology and vaccinology Avian Farms – 1999-2000 Molecular and classical avian virology diagnostics and cell culture Granja Planalto – 1999-2005 Virology diagnostics Peterson Farms – 1998-2000 Molecular and virological diagnostics, laboratory review and design, facility and biosecurity review, personnel review and recommendations Simbios Biotecnologia – 1998-2002 US and International consultations and marketing of avian molecular diagnostics Perdue Inc. – 2004-2005 Molecular and virological diagnostics, laboratory review and design, facility and biosecurity review, personnel review and recommendations Lavetec Productos Veterinarios Ltda. – 1997-2005 Avian virology, vaccine promotion and marketing USDA/FAS - 2005 International avian influenza technical assistance

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