CV: Husain, M Page 1 of 22 06/20/2003

CURRICULUM VITAE

Mansoor Husain A. DATE PREPARED June 20, 2003 B. BIOGRAPHICAL INFORMATION 1. Address: 200 Elizabeth Street, EN 12-221

Toronto, Ontario, Canada M5G-2C4 O:416.340.3188, F:416.340.4021 E-mail: mansoor.husain@utoronto.ca

2. Degrees & Certifications: 1999 Diplomate of the Certification Board of Nuclear Cardiology 1993 Diplomate in Cardiovascular Disease, American Board of Internal Medicine 1993 Certificate of Special Competence in Cardiovascular Medicine, Royal College of Physicians & Surgeons of Canada 1991 Specialty Certificate in Internal Medicine, Royal College of Physicians & Surgeons of Canada 1990 Diplomate in Internal Medicine, American Board of Internal Medicine 1987 Diplomate of the National Board of Medical Examiners 1987 Licentiate of the Medical Council of Canada 1986 Doctoris Medicinae (Honours, Distinction, & Gold Medal) University of Alberta, Edmonton, AB 1984 Bachelor of Medical Science (Honours & Distinction) University of Alberta, Edmonton, AB 3. Employment: a) Academic Appointments: 2001-Present Full Member, Cardiovascular Sciences Collaborative Program, School of

Graduate Studies, University of Toronto 2001-Present Full Member, Institute of Medical Science, University of Toronto 2000-Present Scientist, Toronto General Hospital Research Institute, University Health

Network, Toronto 2000-Present Full Member, Graduate Faculty, Department of Laboratory Medicine and

Pathobiology, University of Toronto 1999-2001 Associate Member, Cardiovascular Sciences Collaborative Program, School of

Graduate Studies, University of Toronto 1998-Present Assistant Professor, Department of Medicine, University of Toronto (with cross-

Appointment to Department of Laboratory Medicine & Pathobiology) 1998-2001 Associate Member, Institute of Medical Science, University of Toronto 1998-2000 Associate Member, Graduate Faculty, Department of Laboratory Medicine &

Pathobiology, University of Toronto 1997-1998 Lecturer, Department of Medicine, University of Toronto 1994-1997 Instructor, Department of Medicine, Harvard Medical School, Boston, MA

CV: Husain, M Page 2 of 22 06/20/2003

1993-1997 Postdoctoral Fellow, Program of Excellence, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA

b) Hospital Appointments: 1999-Present Attending Staff, Dept. of Medicine, Mt. Sinai Hospital, Toronto, ON 1998-Present Associate Staff, Dept. of Radiology, University Health Network, Toronto, ON 1997-Present Attending Staff, Dept. of Medicine, University. Health Network, Toronto, ON 1994-1997 Attending Staff, Dept. of Medicine, Beth Israel Deaconess Medical Center,

Boston, MA 06-09/1993 Associate Staff, Dept. of Medicine, Toronto Hospital, Toronto, ON 1992-1993 Senior Cardiology Resident, Toronto Hospital, Toronto, ON 1991-1992 Junior Cardiology Resident, St. Michael’s Hospital, Toronto, ON 1990-1991 Chief Medical Resident, St. Michael’s Hospital, Toronto, ON 1989-1990 Senior Medical Resident, Toronto Hospital, Toronto, ON 1987-1989 Junior Medical Resident, St. Michael’s Hospital, Toronto, ON 1986-1987 Rotating Interne, St. Michael’s Hospital, Toronto, ON 4. Honours: 2003 Appointment to Board, Canadian Hypertension Society 2003 Appointment to Executive, Corporate Officer, Ontario Hypertension Society 2003 Appointment to Co-Chair, Scientific Review Committee “II”, Heart & Stroke

Foundation of Canada 2002 E. D. Wigle Divisional Teaching Award, University Health Network 1999 Appointment to Scientific Review Committee “CSA”, Canadian Institutes of

Health Research 1998 Appointment to Scientific Review Committee “IVb”, Heart & Stroke Foundation

of Canada 1996 Appointment to Sigma Xi Scientific Research Society, Massachusetts Institute of

Technology 1991 Trainee Award, American Federation of Clinical Research ($1000) 1991 1ST Prize, Higgins' Residents Research Competition, St. Michael’s Hospital 1988 3rd Prize, Higgins' Residents Research Competition, St. Michael’s Hospital 1986 Moshier Memorial Gold Medal, University of Alberta 1986 Douglas E. Leitch Gold Medal in Pediatrics, University of Alberta 1986 E. L. Pope Memorial Award in Medicine, University of Alberta 1986 J. Ross Van Scholarship in Obstetrics & Gynecology, University of Alberta 1985 Bristol Laboratories Pharmacology Award, University of Alberta 1985 University Hospitals Renal Trust Fund Award, University of Alberta ($500) 1981-85 Louise McKinney Scholarships for Acad. Excellence, Govt. of Alberta ($21,000) 1984 Appointment to Alpha Omega Alpha Honour Society, University of Alberta 1983-84 Nat Christie Foundation Awards for Academic Merit, Univ. of Alberta ($3,000) 1980-81 Hudson’s Bay Company University Scholarship ($2,000) 5. Professional Activities & Affiliations: a) Reviewer Status: i) Journals:

CV: Husain, M Page 3 of 22 06/20/2003

2002- Circulation 2001- Arteriosclerosis, Thrombosis & Vascular Biology 2001- Cardiovascular Pathology 2000- Current Drug Targets (CV & Hematological - Editorial Board Member) 1999- Journal of Vascular Surgery (Editorial Board Nomination - pending) 1999- American Journal of Pathology 1998- Pediatric Research 1997- Circulation Research 1997- American Journal of Physiology 1997- Cardiovascular Research ii) Grant Agencies: 2003- Co-Chair, Scientific Review Committee II, Heart & Stroke Foundation Canada 2003- Member, Career Investigator Awards Committee, Alberta Heritage Foundation

for Medical Research 2003- Member, Clinical Investigator Awards Committee, Alberta Heritage Foundation

for Medical Research 2003- External Reviewer, American Alzheimer’s Society 2000- Reviewer, Basic Science Council: Gene Therapy, American Heart Association 2000-2003 Member, Scientific Review Committee “Cardiovascular A”, Canadian Institutes

of Health Research 1998-2000 Member, Scientific Review Committee IVb, Heart & Stroke Foundation of

Canada 1997- External Reviewer, Canadian Institutes of Health Research 1997- External Reviewer, Heart and Stroke Foundation of Canada 1997- External Reviewer, Human Frontier Science Program b) Professional Associations: 2003- Executive, Canadian Hypertension Society 2003- Officer, Ontario Hypertension Society 1999- Member, Canadian Cardiovascular Society 1997- Member, Canadian Hypertension Society 1998- Member, American Society for Nuclear Cardiology 1997- Member, American Federation for Clinical Research 1997- Member, Basic Science & Hypertension Councils, American Heart Association 1996- Member, American Association for the Advancement of Science 1993- Board of Registration in Medicine, Commonwealth of Massachusetts 1991- Member, American College of Cardiology 1991- Fellow, Royal College of Physicians & Surgeons of Canada 1987- Member, American College of Physicians 1987- Member, Ontario College of Physicians & Surgeons 1982- Member, Canadian Medical Association 6. University Committees: 2003- Local Organizing Committee, International Vascular Biology Meeting 2004

CV: Husain, M Page 4 of 22 06/20/2003

2002- Member, Cardiovascular Physiologist Search Committee, Department of Physiology, University of Toronto

1999- Member, Basic Science Committee, Heart & Stroke Foundation of Ontario Richard Lewar Centre of Excellence, University of Toronto

1999-2001 Member, Fitzgerald Building Planning Committee, Heart & Stroke Foundation of Ontario Richard Lewar Centre of Excellence, University of Toronto

1999-2000 Member, Cardiovascular Developmental Biologist Search Commmittee, Department of Pediatrics, Hospital for Sick Children, University of Toronto

1989-1992 Member, On-Site Program Evaluation Team, Postgraduate Education Committee, University of Toronto

7. Hospital Committees 2002- Member, Regenerative Medicine Committee, University Health Network 2000- Member, Space Committee, Toronto General Hospital Research Institute 2000- Chair, Pharmacy and Therapeutics Subcommittee on Management of Acute

Coronary Syndromes, University Health Network 1999- Member, Divisional Research Committee, University Health Network & Mt.

Sinai Hospital 1999- Member, Cardiac Intensive Care Unit Management Committee, University Health

Network & Mt. Sinai Hospital 1999- Member, Equipment Committee, Toronto General Hospital Research Institute,

University Health Network 1998-2000 Member, Animal Care Committee, Toronto General & Western Hospitals,

University Health Network C. ACADEMIC HISTORY 1. Research Endeavors: a) Molecular regulation of vascular smooth muscle cell proliferation and development: Our

studies focus on the role of the c-myb proto-oncogene. We have demonstrated how its encoded product, the c-Myb transcription factor, facilitates G1 to S phase cell cycle transitions in vascular smooth muscle cells (SMC), in part, by down-regulating the transcription of specific plasma membrane Ca2+ ATPase (PMCA1 and PMCA4). This, in turn, enables critical elevations in cell Ca2+ concentrations that are required for cell cycle progression. We have cloned the PMCA1 promoter and have demonstrated its specific structural and functional interactions with c-Myb. We have also recently cloned the mouse PMCA4 gene. We are identifying its promoter, and aim to study the structural basis for its functional regulation during cell cycle. We also study the role of other Ca2+ regulatory genes (SERCA, IP3R) in cell cycle progression and are interested in defining putative Ca2+-responsive cell cycle regulators. In this regard, we have become particularly interested in candidate mediators such as the calcineurin/NFAT pathway, Ca2+/calmodulin-dependent kinases and cdk2/cyclinE. Finally, we have developed an in vitro SMC differentiation schema for mouse embryonic stem cells and are characterizing the transcriptional profiles of differentiating embryoid bodies at specific temporal windows. We aim to employ these techniques for studies on the role of c-myb and calcium regulatory genes during SMC

CV: Husain, M Page 5 of 22 06/20/2003

differentiation. The above studies form the molecular basis of our in vivo experimentation (see below) and have been the specific objectives of my CIHR grants on c-myb.

b) Mouse models of cardiovascular gene regulation and pathobiology: We have developed

transgenic mice possessing arterial SMC-restricted expression of a tetracycline-responsive transcriptional activator (tTA) and have used this system to conditionally express a variety of tTA-dependent transgenes in vivo (Myb-Engrailed: a dominant negative Myb; PMCA1; and PMCA4). We have characterized the function of the tTA system in a wire-mediated denudation model of carotid artery injury and use this model to gauge the physiological importance of transgenes of interest. We have bred our conditional transactivation system into LDL receptor knockout mice (LDLr-/-) in order to study the effects of our transgenes in cholesterol-mediated atherogenesis. The Myb-Engrailed over-expressing animal is dramatically protected from the neointima formation that follows arterial injury in control animals. PMCA4 over-expressing animals are also protected in such a model, but show an additional phenotype that has become the major focus of our HSFO-funded research. Namely, PMCA4 over-expression in arterial SMC results in a unique model of increased blood pressure, which is associated with enhanced myogenic tone and increased sensitivity to endogenous vasoconstrictors. We believe this is due to a heightened pharmaco-mechanical coupling of SMC contraction caused by compensatory increases in the storage of intracellular Ca2+, and decreased activity of neuronal nitric oxide synthse (nNOS) which has been shown to be both structurally and functionally coupled with PMCA4. These two mechanisms are not mutually exclusive and both are being actively pursued. We have also engaged in a number of collaborative projects employing our mouse models of injury and/or conditional expression systems for other transgenes of interest. These include Elafin, an elastase inhibitor and Chymase, an endogenous matrix and zymogen protease (collaborations with Marlene Rabinovitch, Stanford University), and iNOS and Endothelin (collaborations with Duncan Stewart, University of Toronto). The latter two genes have also been expressed in a conditional cardiac-restricted pattern. The cardiac iNOS over-expressing mouse represents a unique model of sudden cardiac death due to a particular propensity of the mouse AV conduction system for iNOS-derived peroxynitrite and/or superoxide. The cardiac endothelin over-expressing animal is a fascinating model of immune cardiomyopathy resulting in heart failure. Both the iNOS and Endothelin projects are part of HSFO and CIHR grant applications from Duncan Stewart and I, and these models will be a major focus of our collaborative research for the next 3 years. Dr Peter Backx (as PI, University of Toronto) and I (as co-PI), have recently been awarded a 5-year CIHR grant for studying the cardiac and vascular function of the ACE2 knockout animal. My role in this project will be to focus on the vascular phenotype of this intriguing mouse. Finally, other collaborations in which we began by characterizing the cardiovascular phenotypes of other genetically engineered mice have revealed unique models of diastolic cardiac dysfunction and cardiac hypertrophy (GLP-1R knockout, in collaboration with Dan Drucker, University of Toronto) and vasomotor dysfunction leading to arteriovenous malformations (Endoglin knockout, in collaboration with Michelle Letarte, University of Toronto). These exciting investigations have been spearheaded by our laboratory and may be developed with our collaborators as distinct submissions for peer-reviewed operating grant support.

CV: Husain, M Page 6 of 22 06/20/2003

c) Cardiac applications of radionuclide imaging: In collaboration with Dr. Robert Mark Iwanochko (University of Toronto), I study the role of 99mTc and/or 201Thal myocardial perfusion imaging with Single Photon Emission Computed Tomography (SPECT) in the diagnosis and prognosis of coronary artery disease. In collaboration with Ren-Ke Li and Richard Weisel (University of Toronto), we also employ SPECT to gauge the viability and function of autologous cardiac cell transplants in a pig model of myocardial infarction.

d) Acute cardiac care: At the Toronto General Hospital, I was principal investigator for the

CURE trial (multicentre randomized placebo-controlled clinical trial of Clopidogrel in unstable angina), and for the Canadian Heart Research Centre’s Registry of Acute Coronary Syndromes. We also study the role of acute physiological scoring systems in the prognosis and management of patients requiring cardiac intensive care.

2. Research Awards: a) Career Support: 2001-2004 Clinician-Scientist Award – Phase II (final renewal), “Molecular regulation of

vascular smooth muscle cell proliferation”, PI: Husain, M. Canadian Institutes of Health Research ($150,000 over 3 years)

1998-2001 Clinician-Scientist Award – Phase II, “Molecular regulation of vascular smooth

muscle cell proliferation”, PI: Husain, M. Canadian Institutes of Health Research ($150,000 over 3 years)

1998-2003 Research Scholarship, “Molecular regulation of vascular smooth muscle cell

proliferation”, PI: Husain, M. Heart & Stroke Foundation of Canada ($300,000 over 5 years – Declined in favor of CIHR award)

1997-1998 Clinician-Scientist Award – Phase I (final renewal), “Molecular regulation of

vascular smooth muscle cell proliferation”, PI: Husain, M (MIT/U of T). Mentor: Rosenberg R. (MIT) /Rabinovitch M (U of T). Medical Research Council of Canada ($43,000 over 1 “transitional” year)

1995-1997 Clinician-Scientist Award – Phase I (first renewal), “Molecular regulation of

vascular smooth muscle cell proliferation”, Trainee: Husain M (MIT). Mentor: Rosenberg R.D (MIT). Medical Research Council of Canada ($86,000 over 2 years)

1993-1995 Clinician-Scientist Award – Phase I, “Molecular regulation of vascular smooth

muscle cell proliferation”, Trainee: Husain M (MIT). Mentor: Rosenberg R. (MIT). Medical Research Council of Canada ($86,000 over 2 years)

1993 Research Fellowship, “Molecular regulation of vascular smooth muscle cell

proliferation”, Trainee: Husain M (MIT). Mentor: Rosenberg R. (MIT).Heart & Stroke Foundation of Canada ($80,000 over 2 years - Declined in favor of MRC award)

CV: Husain, M Page 7 of 22 06/20/2003

1985 Studentship, “NADPH-dependent isocitrate dehydrogenase in Drosophila melanogaster” Trainee: Husain M (U of A). Mentor: Bentley M. (U of C) Alberta Heritage Foundation for Medical Research ($4,500 summer stipend)

b) Operating & Infrastructure Support i) As Principal Investigator: 2003-2008 Canadian Institutes of Health Research Operating Grant: “The role of plasma

membrane Ca2+-ATPase isoforms in vascular smooth muscle cell biology” PI: Husain M. $506,000 over 5 years

2003-2006 Heart & Stroke Foundation of Ontario Operating Grant: “The role of plasma

membrane Ca2+-ATPase isoforms in vascular pathophysiology” PI: Husain M. $243,771 over 3 years

2001-2004 Canadian Institutes of Health Research Operating Grant: “c-Myb-dependent

vascular smooth muscle proliferation” PI: Husain M. $213,639 over 3 years 2000-2003 Heart & Stroke Foundation of Ontario Operating Grant: “Conditional expression

of PMCA isoforms in arterial smooth muscle of transgenic mice” PI: Husain M. $196,325 over 3 years

1999-2002 Ontario Research & Development Challenge Fund New Opportunities Matching

Grant: “Molecular physiology of vascular smooth muscle” PI: Husain M. $200,000 over 3 years for equipment only

1999-2001 University of Toronto Faculty of Medicine Dean’s Fund Grant: “Mouse models of

vascular injury” PI: Husain M. $10,000 over 2 years 1998-2002 Canada Foundation for Innovation New Opportunities Award: “Molecular

physiology of vascular smooth muscle” PI: Husain M. $200,000 over 4 years for equipment only

1998-2001 Medical Research Council of Canada Operating Grant: “c-Myb-dependent

vascular smooth muscle proliferation” PI: Husain M. $198,358 over 3 years 1998-2001 Medical Research Council of Canada Clinician Scientist Award - Phase II

Research Allowance: “Molecular regulation of vascular smooth muscle cell proliferation” PI: Husain M. $120,000 over 3 years

1998-2001 Heart & Stroke Foundation of Ontario Operating Grant: “c-Myb dependent

vascular smooth muscle proliferation” PI: Husain M. $15,000 over 3 years 1998-2000 University of Toronto Connaught Foundation & Department of Medicine New

Staff Grant: “c-Myb dependent vascular smooth muscle proliferation” PI: Husain M. $45,000 over 2 years

CV: Husain, M Page 8 of 22 06/20/2003

ii) As Co-Principal Investigator: 2003-2008 Canadian Institutes of Health Research Operating Grant: “Role of ACE2 in the

regulation of cardiac and vascular function” PI: Backx P, co-PI: Husain M. $561,260 over 5 years

2002-2005 Heart & Stroke Foundation of Ontario Operating Grant: “Targeted over-

expression of inducible NO synthase: relevance for the pathogenesis of cardiovascular disease". PI: Stewart D, co-PI: Husain M $258,000 over 3 years

2001-2004 Canadian Institutes of Health Research Operating Grant: “Pathophysiological

relevance of endothelin-1 over-expression” PI: Stewart D, co-PI: Husain M $331,531 over 3 years

iii) As Group Member: 2000-2005 Canadian Institutes of Health Research Group Grant: “Interdisciplinary Program

in Cardiovascular Development and Disease” Directors: Rabinovitch M/Keeley F; Members: Bruneau B, Hinek A, Husain M, Wilson G; $1,384,531 over 5 years

2000-2005 Heart & Stroke Foundation of Ontario Group Grant: “Cell Biology of

Atherosclerosis” Director: Langille L; Members: Bendeck M, Cybulsky M, Gotlieb A, Husain M, Marsden P, Strauss B; $784,898 over 5 years

2000-2003 Canadian Institutes of Health Research Group Grant: “The Determinants of Host

Susceptibilities of Heart Failure" Director: Dr. Liu P; Members: Backx P, Parker T, Rabinovitch M, Rouleau JL, Stewart D/Husain M; $1,242,952 over 3 years

b) Supervisor Support: i) Special Recognition: 2003-2007 Premier’s Research Excellence Award, “Molecular regulation of vascular

smooth muscle cell proligferation: the role of calcium pumps in atherosclerosis and hypertension” PI: Husain, M. Government of Ontario ($150,000 over 4 years for stipend support of graduate and/or postdoctoral students)

ii) Postdoctoral Fellowship Awards: 2001-2002 Ehsan Al-Hoque, PhD; Department of Medicine, Postdoctoral Fellowship:

“Intracellular Ca2+ dynamics in freshly isolated aortic smooth muscle cells from transgenic mice” $20,000 over 1 year

2000-2001 Talat Afroze, PhD; Heart & Stroke Richard Lewar Centre of Excellence Postdoctoral Fellowship: “Cell cycle-dependent regulation of Ca2+ pumps in vascular smooth muscle cells” $20,000 over 1 year

2000-2002 Robert Gros, PhD; Heart & Stroke Foundation of Ontario Postdoctoral Fellowship: “Hypertension models related to disturbed intracellular Ca2+ regulation” $66,000 over 2 years

2000 Robert Gros, PhD; Heart & Stroke Foundation of Ontario Evelyn McGloin Fellowship: “Hypertension models related to disturbed intracellular Ca2+ regulation” $5,000 over 1 year

CV: Husain, M Page 9 of 22 06/20/2003

2000 Robert Gros, PhD; Edward Christie Stevens Fellowship in Medicine: “Hypertension models related to disturbed intracellular Ca2+ regulation” $6,000 over 1 year

1999-2001 Xiaomang You, MD; Medical Research Council of Canada & Canadian Hypertension Society Postdoctoral Fellowship: “Transgenic expression of a dominant negative c-Myb” $66,000 over 2 years

iii) Graduate Studentship Awards: 2004-2007 Karolina Kolodziejska-Baginska, HSFO Doctoral Studentship, “Role of c-Myb in

the in vitro differentiation of vascular smooth muscle cells from mouse embryonic stems cells” $20,000/year

2003 Karolina Kolodziejska-Baginska, OGSST Studentship, “Role of c-Myb in the in vitro differentiation of vascular smooth muscle cells from mouse embryonic stems cells” $15,000 over 1 year

2003 Jae Hyun Choi, OGSST Studentship, “Mechanisms underlying Ca2+-dependent cell cycle progression in vascular smooth muscle cells” $15,000 over 1 year

2002 Karolina Kolodziejska-Baginska, Connaught Scholarship, “Role of c-Myb in the in vitro differentiation of vascular smooth muscle cells from mouse embryonic stems cells” $25,000 over 1 year

2002 Jae Hyun Choi, IMS Continuing Fellowship Award, “Mechanisms underlying Ca2+-dependent cell cycle progression in vascular smooth muscle cells” $7,500 over 1 year

2002 Ge Yang, Heart & Stroke Richard Lewar Centre of Excellence Fellowship, “Cloning and characterization of the mouse PMCA4 promoter” $15,000 over 1 year

2002-2004 Li Yang, Heart & Stroke Foundation of Canada Graduate Studentship: “Conditional cardiovascular over-expression of endothelin-1 in transgenic mice” $40,060 over 2 years

2001 Li Yang, Heart & Stroke Richard Lewar Centre of Excellence Graduate Studentship: “Conditional cardiovacular over-expression of endothelin-1 in transgenic mice” $15,000 over 1 year

2000-2002 Imran Mungrue, Heart & Stroke Foundation of Ontario Ph.D. Graduate Studentship: “Regulated over-expression of inducible nitric oxide synthase (iNOS) in the cardiovascular system of transgenic mice” $38,000 over 2 years

iv) Summer Studentship Awards: 2003 Shivalika Handa, Life Sciences Award, Cardiovascular Sciences Collaborative

Program “Role of iNOS ni myocardial infarction in mice” $1,500 over 1 summer 2003 Bheeshma Ravi, Canadian Hypertension Society Summer Studentship “Role of

G-protein-coupled receptors in hypertension in mice” $3,300 over 1 summer 2003 Asif Pirani, Faculty of Medicine Summer Studentship, “Inducible NOS over-

expression in arterial SMC: what is mediating the hypertension?” $3,500 over 1 summer

2003 Purti Papneja, John D. Schultz Science Student Scholarship, Heart & Stroke Foundation of Ontario: “Gender, development and disease-induced changes in the signal-averaged ECG of mice” $4,000 over 1 summer

CV: Husain, M Page 10 of 22 06/20/2003

2002 Asif Pirani, Canadian Hypertension Society Summer Studentship, “Role of iNOS in vasomotor regulation in mesenteric arteries” $3,300 over 1 summer

2002 Toby Chan, John D. Schultz Science Student Scholarship, Heart & Stroke Foundation of Ontario: ““Characterization of signal-averaged ECG in a mouse model of myocardial infarction” $4,000 over 1 summer

2002 Bheeshma Ravi, Life Sciences Award, Cardiovascular Sciences Collaborative Program “Use of a mouse model of carotid injury to study c-Myb-dependent VSMC proliferation in vivo” $1,500 over 1 summer

2001 Hashmat Khan, Life Sciences Award, Institute of Medical Sciences, University of Toronto: “Acute Physiological Scoring Systems in Congestive Heart Failure” $1,800 – over 1 summer. Winner, Abstract Prize - Summer Student Poster Presentation Day

2001 Asif Pirani, Life Sciences Award, Cardiovascular Sciences Collaborative Program: “Histomorphological characterization of the atrioventricular node and His Purkinje fibres in the mouse” $1,500 over 1 summer

2001 Jasmine Cheung, John D. Schultz Summer Studentship Award, Heart & Stroke Foundation of Ontario: “Cloning of the mouse PMCA4 promoter” $3,500 over 1 summer

2001 Toby Chan, Summer Studentship Award, Canadian Hypertension Society: “Characterization of signal-averaged ECG in wild-type and mutant mice” $3,500 over 1 summer

2001 Daniel Schiff, Summer Studentship Award, Faculty of Medicine, University of Toronto: “Ca2+-dependent activity of specific G1/S cell cycle proteins” $3,500 over 1 summer

2000 Ryan Van Wert, Heart & Stroke Foundation of Ontario John D. Schultz Summer Studentship Award: “Gender and age-related differences in myogenic tone in murine arterial beds” $3,500 over 1 summer

2000 Tara Hyder, Canadian Hypertension Society Summer Studentship: “Ca2+-dependent cell cycle kinase activity in the late G1 to S phase of vascular smooth muscle cells” $3,300 over 1 summer

2000 Don Mehra, Heart & Stroke Foundation of Ontario Martin Wills Summer Studentship: “Image analysis of migrating arterial SMC in vivo” $2,000 over 1 summer

2000 Anil Misir, Heart & Stroke Foundation of Ontario John D. Schultz Summer Studentship Award: “Ca2+ measurements in freshly dispersed smooth muscle cells from a single mouse aorta” $3,500 over 1 summer

1999 Ryan Van Wert, Canadian Hypertension Society Summer Studentship: “Vasomotor tone in animals over-expressing PMCA pumps” $3,300 over 1 summer

1999 Tara Hyder, University of Toronto Life Sciences Summer Studentship Award: “Does hypoxia affect VEGF-mediated signal transduction in vascular smooth muscle cells?” $1,500 over 1 summer

1999 Bheeshma Ravi, Heart & Stroke Foundation of Ontario Martin Wills Summer Studentship: “Morphometry of carotid artery injuries” $2,000 over 1 summer

CV: Husain, M Page 11 of 22 06/20/2003

D. PUBLICATIONS 1. Peer-Reviewed a) Original Research Papers:

1. Gros R, You X, Hoque ANE, Kalair W, Afroze T, Mungrue I, Husain M: Conditional overexpression of plasma membrance Ca2+ ATPase 4b in arterial smooth muscle of transgenic mice. Circ Res 2003 in press (Senior Responsible Author, Supervisor of all other authors)

2. Gros R, You X, Baggio L, Kabir MG, Mungrue IN, Parker TG, Huang Q, Sadi AM,

Drucker DJ, Husain M. Cardiac function in mice lacking the glucagon-like peptide-1 receptor. Endocrinology 2003; 144(6):2242-52 (Senior Responsible Author, Supervisor of Gros, You, Kabir, Mungrue, Sadi)

3. Afroze T, Yang LL, Wang C, Gros R, Kalair W, Hoque ANE, Mungrue I, Zhu Z,

Husain M: Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in mouse vascular smooth muscle cell cycle. Am J Physiol: Cell Physiol 2003; 285(1): C88-95 (Senior Responsible Author, Supervisor of all co-authors)

1. You X, Mungrue I, Kalair W, Afroze T, Ravi B, Gros R, Husain M: Conditional

expression of a dominant negative c-Myb in vascular smooth muscle cells inhibits arterial remodeling following injury. Circ Res 2003; 92: 314-321 (Senior Responsible Author, Supervisor of all other authors)

2. Shakil T, Hoque ANE, Husain M, Belsham DD: Differential regulation of GnRH

secretion and gene expression by androgen: Membrane versus nuclear receptor activation. Mol. Endocrinol., 2002; 16(11):2592-602 (Collaborator, Supervisor of Hoque)

3. Mungrue IN, Gros R, You X, Pirani A, Azad A, Csont T, Schultz R, Butany J, Stewart

DJ, Husain M: Cardiomyocyte over-expression of human inducible nitric oxide synthase in mice results in peroxynitrite generation, heart block and sudden death. J. Clin. Invest. 2002; 109: 735-743 (Co-Senior Responsible Author, Supervisor of Mungrue, Gros, You, Pirani)

4. Emmett L, Iwanochko, RM, Barolet A, Freeman M, Lee D, Husain M: Reversible

regional wall motion abnormalities on exercise Tc-99m gated cardiac SPECT predict high-grade angiographic stenoses. J. Am. Coll. Cardiol. 2002; 39: 991-998 (Senior Responsible Author, Co-Supervisor of Emmett)

5. Gros R, Van Wert R, You X, Thorin E, Husain M: Age, gender and blood-pressure-

dependent myogenic responses in C57Bl/6 mice. Am. J. Physiol.: Heart & Circ. Physiol. 2002; 282(1): H380-H388 (Senior Responsible Author, Supervisor of Gros, Van Wert, You)

CV: Husain, M Page 12 of 22 06/20/2003

6. Zaidi SHE, You X, Ciura S, Husain M, Rabinovitch M. Overexpression of the serine elastase inhibitor Elafin protects transgenic mice from hypoxic pulmonary hypertension. Circulation 2002; 105: 516-521 (Collaborator, Supervisor of You)

7. Ju, H., Gros, R., You, X., Tsang, S., Husain, M. and Rabinovitch, M. Conditional and

targeted overexpression of vascular chymase in transgenic mice causes hypertension. Proc. Natl. Acad. Science USA 2001; 2001; 98: 7469-7474 (Collaborator, Supervisor of Gros, You)

8. Zaidi SHE, You X, Ciura S, O'Blenes S, Husain M, Rabinovitch M: Suppressed Smooth

Muscle Proliferation and Inflammatory Cell Invasion Following Arterial Injury in Elafin-Overexpressing Mice. J. Clin. Invest. 2000; 105: 1687-1695 (Collaborator, Supervisor You)

9. Li RK. Weisel RD. Mickle DA. Jia ZQ. Kim EJ. Sakai T. Tomita S. Schwartz L.

Iwanochko M. Husain M. Cusimano RJ. Burns RJ. Yau TM: Autologous porcine heart cell transplant-ation improved heart function after a myocardial infarction. J. Thor. Cardiovasc. Surg. 119(1):62-8, 2000 (Collaborator)

10. Afroze T, Husain M: c-Myb binding sites mediate G1/S-associated repression of the

plasma membrane Ca2+ ATPase-1 promoter. J. Biol. Chem. 2000; 275: 9062-9069 (Senior Responsible Author, Supervisor of Afroze)

11. Zaidi SHE, Hui C, Cheah AYL, You X, Husain M, Rabinovitch M: Transgenic mice

with targeted overexpression of the elastase inhibitor Elafin are protected against encephalo-myocarditis virus-induced myocardial dysfunction and mortality. J. Clin. Invest. 1999; 103: 1211-1219 (Collaborator, Supervisor of You)

12. Husain M*, Bein K*, Ware A, Rosenberg RD, Simons M: c-Myb function in 3T3

fibroblasts. J. Cell Physiol 1997; 173:319-326 (* = equal contribution) (Principal Author)

13. Husain M, Jiang L, See V, Bein K, Simons M, Alper S, Rosenberg RD: Regulation of

vascular smooth muscle cell proliferation by plasma membrane Ca2+ -ATPase. Am. J. Physiol. (Cell) 1997; 272: C1947-1959 (Principal Author)

14. Husain M, Bein K, Jiang L, Alper S, Simons M, Rosenberg RD: c-Myb dependent cell

cycle progression and Ca2+ storage in cultured vascular smooth muscle cells. Circ. Res. 1997; 80: 617-626 (Principal Author)

15. Weiler-Guettler H, Aird WC, Husain M, Rayburn H, Rosenberg RD: Targeting of

transgene expression to the vascular endothelium of mice by homologous recombination at the thrombomodulin locus. Circ. Res. 1996; 78: 180-187 (Collaborator)

CV: Husain, M Page 13 of 22 06/20/2003

16. Weiler-Guettler H, Aird WC, Rayburn H, Husain M, Rosenberg RD: Developmentally regulated gene expression of thrombomodulin in potsimplantation mouse embryos. Development 1996; 122: 2271-2281 (Collaborator)

17. Husain M, Armstrong PW, Connelly PW, Hegele RA: Lipoprotein(a) and

apolipoproteins B and A-I after acute myocardial infarction. Can. J. Cardiol. 1995; 11: 206-210 (Principal Author)

18. Husain M, Freedman JJ, Hegele RA: A possible escape phenomenon of lipoprotein(a) in

sustained plasma exchange. Transfus. Sci. 1993; 14: 417-421 (Principal Author) b) Review Articles or Chapters in Books:

21. Mungrue IN, Bredt DS, Stewart DJ, Husain M: From Molecules to Mammals: What’s NOS got to do with it?” Acta Physiol Scand 2003 in press (Co-Senior Responsible Author, Supervisor of Mungrue)

22. Yang L, Husain M, Stewart DJ: The Role of Endothelin-1 in Myocardial Inflammation

and Fibrosis. Book Chapter in “Inflammation and Cardiac Diseases” 2003 in press Birkhauser Verlag Switzeland. (Co-Senior Responsible Author, Co-Supervisor of Yang)

23. Arab S, Husain M, Liu P: Principles and methods of cDNA microarrays in heart failure

research. Book Chapter: 2003 in press (Collaborator) 24. Afroze T, Husain M:” c-Myb-mediated transcription of multiple Ca2+ transporters at the

G1/S transition point of vascular smooth muscle cells”. Recent Res. Devel. Biol. Chem. 2002; 273-285 (ISBN 81-7736-153-8) (Senior Responsible Author, Supervisor of Afroze)

25. Mungrue IN, Husain M, Stewart DJ: The role of NOS in heart failure: lessons from

murine genetic models. Heart Fail Rev. 2002; 7(4):407-22 (Co-Senior Responsible Author, Supervisor of Mungrue)

26. Afroze T, Husain M: Cell cycle-dependent regulation of intracellular calcium

concentration in vascular smooth muscle cells: A potential target for drug therapy. Curr Drug Targets – Cardiovasc & Haematol Dis 2001; 1: 23-40 (Senior Responsible Author, Supervisor of Afroze)

27. Husain M, Simons M: Vascular antisense targets: c-myc, c-myb and PCNA. Book

Chapter in “Applications of antisense therapies to restenosis” Kluwer Academic Publishers: 1999; 71-98 (Principal & Senior Responsible Author)

c) Abstracts:

28. Gattas DJ, Kabir G, Parker TG, Husain M, Sibbald WJ; Echocardiography and invasive pressure monitoring in vivo can demonstrate adaptive changes in myocardial function in a mouse model of sepsis. American Thoracic Society – ATS 2003 Seattle in press (Co-Senior Responsible Author, Supervisor of Gattas, Kabir)

CV: Husain, M Page 14 of 22 06/20/2003

29. El-Moselhi AB, Yang LL, Gros R, Kabir MG, Proteau G, Stewart DJ, Husain M: Conditional over-expression of human endothelin-1 in arterial SMC of mice causes transient hypertension. FASEB J. 2003 17(4) A535 (Co-Senior Responsible Author, Supervisor of Moselhi, Yang, Kabir)

30. Sadi AM. Kabir MG, Chan T, Gros R, Husain M; Rapid remodeling of arteries in L-

NAME-induced hypertension in mice. FASEB J. 2003 17(4) A271 (Senior Responsible Author, Supervisor of Sadi, Kabir, Chan, Gros)

31. Kabir MG, Chan T, Pirani A, Sadi AM, Mungrue IN, Gros R, Husain M; The superoxide

scavenger Tempol reduces myocardial infarct size and mortality in mice. FASEB J. 2003 17(5) A881 (Senior Responsible Author, Supervisor of Kabir, Chan, Pirani, Sadi, Mungrue, Gros)

32. Yang L, Gros R, Kabir G, Gotlieb AI, Husain M, Stewart DJ; Conditional cardiac over-

expression of endothelin-1 in transgenic mice causes a fatal inflammatory cardiomyopathy. Circulation 2002; 106(19): II-160 Winner of a Trainee Award (Co-Senior Author, Supervisor of Yang, Gros, Kabir)

33. Gros R, You X, Van Wert R, Afroze T, Hoque E, Husain M. Overexpression of plasma

membrane Ca2+ ATPase in arterial smooth muscle cells of transgenic mice increases blood pressure. Circulation 2002; 106(19): II-432 (Senior Author, Supervisor of Gros, You, Van Wert, Afroze, Hoque)

34. Mungrue IN, Gros R, You X, Husain M & Stewart DJ. Impaired endothelium-dependent

vasodilatation in mice with arterial myocyte over-expression of inducible nitric oxide synthase (iNOS) is reversed with L-arginine and tetrahydrobiopterin. Circulation 2002; 106(19): II-212 (Co-Senior Author, Supervisor of Mungrue, Gros, You)

35. Micevski V., Lee DS., Dzavik V., Husain M., Iwanochko RM. Gender-bias in the

referral patterns for coronary angiography following myocardial perfusion imaging. Can J Cardiol 2002, 18(Suppl B),183B (Co-Author, Supervisor of Lee)

36. Gattas DJ, Tsoporis JN, Yu P, Parker TG, Husain M, Sibbald WJ. The role of re-

expressed fetal genes and inducible nitric oxide synthase in the myocardial response to sepsis. American Thoracic Society - ATS 2002 Atlanta. May 2002. (Co-Senior Responsible Author & Supervisor of Gattas)

37. Micevski V, Lee DS, Dzavik V, Husain M, Iwanochko RM. Gender-bias in referral

patterns for cardiac catheterization following myocardial perfusion imaging. European J Nucl Med & Molecular Imaging, 2002; 29: S98. (Co-Author, Supervisor of Lee)

38. Lee D, Emmett L, Parker J, Husain M, Lumsden C, Heeney T, Mullner E. Iwanochko

RM. Thallium infusion myocardial imaging predicts increased mortality in patients with ischemic cardiomyopathy. Circulation 2001; 104 (II):2771 (Co-Author, Supervisor of Lee, Emmett)

CV: Husain, M Page 15 of 22 06/20/2003

39. Zaidi SHE, You X, Ciura S, Husain M, Rabinovitch M. Endogenous overexpression of

the serine elastase inhibitor Elafin protects transgenic mice from hypoxic pulmonary hypertension. Circulation 2001; 104 (17): 2498 (Collaborator, Supervisor of You)

40. Yang L, Husain M, Stewart D. Conditional cardiac overexpression of human endothelin-

1 induces cardiac hyperthrophy and inflammation in transgenic mice. Can J Cardiol 2001; 17: 173C (Co-Principal Author, Co-Supervisor of Yang) 1st Prize Basic Science Student Award

41. Afroze T, Wang C, Yang, L, Kalair W, Zhu Z, Husain M. Calcineurin Reinforces the

Ca2+ Signal at the G1/S Transition in the Vascular Smooth Muscle Cell Cycle. Can J Cardiol 2001; 17: 186C (Senior Responsible Author, Supervisor of Afroze, Wang, Yang, Kalair, Zhu)

42. Gattas DJ, Yu P, Husain M, Sibbald WJ. Myocardial Adaptation to Sepsis is Associated

with the Expression of Fetal Genes, Antioxidant Defenses, and Apoptosis. [P98] The 8th World Congress of Intensive and Critical Care Medicine, Sydney. October 2001. (Co-Senior Responsible Author & Supervisor of Gattas)

43. Gros R, You X, Van Wert R, Husain M. Overexpression of plasma membrane Ca2+

ATPase in arterial smooth muscle cells of transgenic mice is associated with increased blood pressure and vasoconstriction. FASEB J 2001; 15(4): A445. Winner of an AFMR Trainee Award (Senior Responsible Author, Supervisor of Gros, You, Van Wert)

44. Gros R, Van Wert R, You X, and Husain, M. Age and blood pressure-dependent decline

in myogenic tone development in resistance arteries of C57BL/6 mice. FASEB J 2001; 15(4): A774. Winner of an AFMR Trainee Award (Senior Responsible Author, Supervisor of Gros, You, Van Wert)

45. Mungrue I, Gros R, You X, Azad A, Stewart DJ, Husain M. Myocardial over-expression

of inducible nitric oxide synthase in mice models of human heart disease. FASEB J 2001; 15(4): A783. Winner of an APS “Novel Models of Disease Award” (Co-Senior Author, Supervisor of Mungrue, Gros, and You)

46. Afroze T, Husain M: c-Myb-dependent activation of the IP3R1 promoter. FASEB 2001;

15(4):A80 Winner of an AFMR Trainee Award (Senior Responsible Author, Supervisor) 47. Yang L, Husain M, Stewart D: Conditional cardiac overexpression of Endothelin-1 in

transgenic mice. FASEB J. 2001; 15 (5) A1138 (Co-Principal Author & Supervisor) 48. Emmett L, Iwanochko RM, Barolet AW, Husain M. Correlation of rest-stress gated

SPECT-determined regional wall motion abnormalities with coronary angiographic stenoses. J. Nuc. Cardiol. 2001 in press (Co-Senior Responsible Author, Co-Supervisor of Emmett)

CV: Husain, M Page 16 of 22 06/20/2003

49. Ju, H., You, X., Gros, R., Tsang, S., Husain, M. and Rabinovitch, M. Conditional and targeted overexpression of vascular chymase in transgenic mice causes hypertension. Mol Biol Cell 2000; 11 (Suppl S): 234A (Collaborator, Supervisor of You, Gros)

50. Emmett L, Husain M, Iwanochko M: Electrocardiographic abnormalities predict the

severity of posterior wall perfusion defects on 99mTc-Sestamibi myocardial perfusion imaging. Can. J. Cardiol. 2000 in press (Co-Principal Author, Co-Supervisor of Emmett)

51. Nanthakumar N, Burns R, Husain M, Iwanochko M: Stress electrocardiography does not

predict myocardial ischemia in post bypass patients. Pace 2000; in press (Co-Principal Author, Co-Supervisor of Nanthakumar)

52. Zaidi SHE, You X, Husain M, Rabinovitch M: Suppression of elastase activity protects

from medial/intimal thickening following carotid injury in Elafin-overexpressing transgenic mice. Pediatric Research 2000: 47(4)-2:53A (Collaborator, Supervisor of You)

53. Mungrue I, Gros R, You X, Stewart D, Husain M: Overexpression of inducible nitric

oxide synthase in arterial smooth muscle cells of transgenic mice. Circulation 2000: 102: II-151 (Senior Responsible Author, Supervisor of Mungrue, Gros, You)

54. Ju H, You X, Husain M, Rabinovitch M: Targeted overexpression of vascular smooth

muscle cell chymase in transgenic mice causes hypertension. Circulation 2000: 102: II-96 (Collaborator, Supervisor of You)

55. Ohta K, Zaidi SHE, Cheah AYL, Dawood F, Liu P, You X, Husain M, Rabinovitch M:

Overexpression of elafin protects against acute left ventricular dilatation and diastolic dysfunction after experimental myocardial infarction in mice. Circulation 2000: 102: II-357 (Collaborator, Supervisor of You)

56. Mungrue I, Husain M, You X, Azad A, Stewart D: Heart failure induced by conditional

overexpression of inducible NO-synthase targeted to the heart of transgenic mice. Acta Physiol Scand 1999; 167 (Suppl 645): 33 (Co-Principal Author, Supervisor of Mungrue, You)

57. Afroze T, Husain M: A functional c-Myb and intact c-Myb binding sites mediate cell

cycle-dependent repression of the plasma membrane Ca2+ ATPase-1 gene in vascular SMC. Circulation 1999:100: I-844 (Senior Responsible Author, Supervisor of Afroze)

58. Cheah AYL, Zaidi SHE, You X, Dawood F, Wen W, Husain M, Liu P, Rabinovitch M:

Transgenic mice overexpressing the serine elastase inhibitor elafin and experimental myocardial infarct following coronary artery ligation. Circulation 1999: 100: I-250 (Collaborator, Supervisor of You)

CV: Husain, M Page 17 of 22 06/20/2003

59. Mungrue I, Stewart D, You X, Azad A, Husain M: Myocardial overexpression of human inducible nitric oxide synthase in transgenic mice is cardiotoxic. Circulation 1999: 100: I-112 (Senior Responsible Author, Supervisor of Mungrue, You)

60. You X, Mungrue I, Afroze T, Husain M: Transgenic expression of a dominant negative

c-Myb in arterial smooth muscle cells of mice reduces intimal-medial thickening following carotid artery injury. Circulation 1999:100: I-547 (Senior Responsible Author, Supervisor of You, Mungrue, Afroze)

61. Somani Z, Burke R, Husain M, Iwanochko RM: A comparative assessment of the

sensitivity of Cedar Sinai and Emory cardiac toolbox quantitative gated SPECT software to changes in reconstruction filtering parameters. J Nuc Med 2000:in press (Co-Senior Responsible Author)

62. You X, Afroze T, Mungrue I, Backx P, Husain M: Overexpression of plasma membrane

Ca2+ ATPase-4 in arterial smooth muscle cells of transgenic mice inhibits intimal-medial thickening following carotid artery injury. Circulation 1999: 100: I-546 (Senior Responsible Author, Supervisor of You, Mungrue, Afroze)

63. Zaidi SHE, You X, Husain M, Ciura S, O’Blendes S, Rabinovitch M: Transgenic mice

overexpressing the serine elastase inhibitor elafin are protected against carotid arterial injury. Circulation 1999: 100: I-752 (Collaborator, Supervisor of You)

64. Afroze T, Husain M: Increased expression of the IP3R may contribute to the rising

cytosolic Ca2+ concentrations of proliferating SMC. Can. J. Cardiol. 1999: 15: 107D (Senior Responsible Author, Supervisor of Afroze)

65. Mungrue I, Stewart D, You X, Azad A, Husain M: Myocardial expression of human

inducible nitric oxide synthase in transgenic mice. Can. J. Cardiol. 1999: 15: 116D (Finalist in CSAVB Young Investigator Competition) (Senior Responsible Author, Supervisor of You, Mungrue)

66. Mungrue I, Stewart D, You X, Azad A, Husain M: Regulated expression of human

inducible nitric oxide synthase in arterial myocytes of mice. Can. J. Cardiol. 1999: 15: 107D (Senior Responsible Author, Supervisor of You, Mungrue)

67. Raza I, Siu S, Daly P, Husain M: Physiological scoring and IABP usage predict acute

mortality in CHF patients admitted to a cardiac ICU. Can. J. Cardiol. 1999: 15: 230D (Senior Responsible Author, Supervisor of Raza)

68. Choi R, Yu E, Husain M, Iwanochko RM: Correlation of segmental perfusion and wall

motion in 99mTc gated-SPECT myocardial perfusion imaging. Can. J. Cardiol. 1999: 15: 186D (Co-Principal Author, Co-Supervisor of Choi)

69. Li RK, Weisel RD, Mickle DAG, Yau TM, Burns RJ, Iwanochko RM, Husain M,

Schwartz L, Kim EJ, Sakai T, Tomita S, Boylen P, Jia ZQ: Autologous cardiomyocyte

CV: Husain, M Page 18 of 22 06/20/2003

transplantation improved pocine heart function after myocardial infarction. Can. J. Cardiol. 1999: 15: 214D (Collaborator)

70. Afroze T, Husain M: c-Myb-mediates cell cycle-dependent transcriptional repression of

PMCA1. FASEB 1999; 13: A437 (Winner of the AFMR’s 1999 Henry Christian Award) (Senior Responsible Author, Supervisor of Afroze)

71. You X, Mungrue I, Scrocchi L, Drucker D, Husain M: Altered hemodynamics in mice

lacking the glucagon-like peptide 1 receptor. FASEB 1999; 13: A109 (Winner of a FASEB Travel Award) (Senior Responsible Author, Supervisor of You, Mungrue)

72. Zaidi SHE, Hui CC, Husain M, You X, Rabinovitch M: Targeted overexpression of the

serine elastase inhibitor Elafin in transgenic mice reduces injury induced vascular remodelling. Mol Biol Cell 1998; 9:6a: 31 (Collaborator, Supervisor of You)

73. deYang L, Iwanochko RM, Heeney T, Husain M, Burns RJ: Enhanced myocardial

reversibility by continuous thallium infusion. Can J. Cardiol 1998: 14: 125F (Collaborator)

74. Husain M, Olson E, Rosenberg RD: Conditional transgene regulation in arterial smooth

muscle cells in mice. Circulation 1997: 96: I-501 (Principal Author)

75. Husain M, See V, Jiang L, Alper SL, Rosenberg RD: Overexpression of plasma membrane calcium ATPase-1 reduces intracellular calcium and slows growth of vascular smooth muscle cells. Circulation 1996; 94: I-107 (Principal Author, Supervisor of See)

76. Husain M, Jiang L, Bein K, Ware JA, Alper SL, Simons M, Rosenberg RD: Dominant

negative c-Myb constructs lower intracellular calcium levels and slow cell cycle progression in vascular smooth muscle cells. Circulation 1995; 92: I-299 (Principal Author)

77. Husain M, Koo EWY, Gotlieb AI: Endothelial necrosis and basic FGF: Early features of

neointimal formation in the porcine aortic organ culture. Can. J. Cardiol. 1993; 9: 93E (Principal Author)

78. Husain M, Hegele RA, Connelly PW, Armstrong PW: Evolution of Lp(a) and

Apolipoproteins B and A-I after acute myocardial infarction. J. Am. Coll. Cardiol. 1993; 21: 239A (Principal Author)

79. Husain M, Freedman JJ, Hegele RA: An escape phenomenon of lipoprotein(a) in

sustained plasma exchange. Clin. Research 1991;92: 666A (Winner of an AFMR Travel Award) (Principal Author)

2. Non-Peer-Reviewed

CV: Husain, M Page 19 of 22 06/20/2003

1. Husain M, Morgan J (eds): The utility of intravenous nitroglycerin therapy in a rural setting. Alberta Heart Association Coronary Care Newsletter. Spring 1985 (Principal Author)

2. Husain M, Morgan J (eds): The significance of premature ventricular beats. Alberta

Heart Association Coronary Care Newsletter. Spring 1985 (Principal Author) 3. Manuscripts Submitted

4. Yang L, Kabir G, Gros R, El-Moselhi A, Husain M, Stewart D: Conditional cardiac-specific overexpression of ET-1 in transgenic mice results in an inflammatory cardiomyopathy and heart failure. REVISED MANUSCRIPT submitted to Circulation 2003 (Corresponding and Co-Senior Responsible Author, Supervisor of Yang, Kabir, Gros, El-Moselhi)

4. Manuscripts in Revision or Preparation:

1. Husain M, You X, Mungrue I, Afroze T, Gros R, See V, Lech M, Rosenberg R: SM22α promoter-defined conditional transgene expression in smooth muscle cells of mice. (Principal Author, Supervisor of You, Mungrue, Afroze, Gros, See)

2. Emmett L, Husain M, Lee DS, Freeman M, Barolet A, Iwanochko, RM: Reduced

detection of triple vessel and left main coronary artery disease with attenuation corrected myocardial perfusion imaging (Corresponding Author, Co-Supervisor of Emmett)

3. Khan H, Siu S, Raza I, Daly P, Ross H, Husain M: Acute physiological scoring systems

and intra-aortic balloon pump usage predict mortality in congestive heart failure patients admitted to a cardiac intensive care unit. (Senior Responsible Author, Supervisor of Khan, Raza)

4. Emmett L, Husain M, Iwanochko, RM: Electrocardiographic abnormalities predict the

severity of posterior wall perfusion defects on 99mTc-Sestamibi myocardial perfusion imaging. (Co-Principal Author, Co-Supervisor of Emmett)

5. Petrovici R, Nanthakumar K, Iwanochko, RM, Husain M: Value of exercise

electrocardiography following aorto-coronary bypass surgery. (Co-Senior Responsible Author, Supervisor of Petrovici, Nanthakumar)

6. Husain M, See V, Rosenberg RD: Structure of the mouse gene for AT-III (Principal

Author, Supervisor of See)

D. SPECIAL PRESENTATIONS & INVITED LECTURES 1). Local:

1. “Modeling hypertension in the mouse: trick or treat” Toronto CIHR Group Grants Seminar Series (Oct 31, 2002)

CV: Husain, M Page 20 of 22 06/20/2003

2. “Over-expression of plasma membrane Ca2+ ATPase in arterial smooth muscle of transgenic mice: serendipitous challenges of an unexpected phenotype" Inter-Disciplinary Research Seminar, Hospital for Sick Children, Toronto (March 7, 2002)

3. “Cardiovascular Effects of iNOS: mouse models of surprising complexity” Department of Medicine, Grand Rounds, University Health Network, Toronto (July 26, 2001)

4. “Transcription factors and calcium pumps: molecular studies of smooth muscle cell proliferation” Toronto General Hospital Research Institute (Jan 17, 2001)

5. “Endothelial dysfunction: Can it be reversed?” Parke-Davis Symposium on ACE Inhibitors and Endothelial Dysfunction, Collingwood, ON (Feb 19, 2000)

6. “Molecular biologist performs coronary artery bypass: New England Journal of Medicine or National Enquirer?” Cardiology Rounds, William Osler Health Centre, Brampton, ON (Oct 27, 1999)

7. “Transgenic overexpression of PMCA4b in arterial smooth muscle cells” Cardiology Research Rounds, St. Michael’s Hospital, Toronto, ON (Jun 7, 1999)

8. “Transgenic overexpression of PMCA4b in arterial smooth muscle cells: an unexpected phenotype” Seminar, Department of Physiology, University of Toronto, Toronto, ON (May 7, 1999)

9. “Myocardial revascularization: molecular approaches to angiogenesis” Grand Medical Rounds, North York General Hospital, North York, ON (April 22, 1999)

10. “Transgenic tools for cardiovascular research” Cardiology Research Rounds, Toronto General Hospital, Toronto, ON (Mar 2, 1999)

11. “The role of c-Myb in vascular smooth muscle cell proliferation” Vascular Biology Seminars, Hospital for Sick Children, Toronto (Feb 18, 1999)

12. “Myocardial revascularization: molecular approaches to angiogenesis” Cardiac Care Rounds, Centenary Health Centre, Toronto (Feb 16)

13. “State-of-the-Art Overview: Myocardial Revascularization” Sunnybrook Health Sciences Centre CME Event “Contemparary Cardiac Care”, Deerhurst (Sep 19, 1998)

14. “Transgenic studies of cardiovascular physiology” Scientific Day, Institute of Medical Science, University of Toronto (April 14, 1998)

15. “Molecular biology of the platelet GpIIb/IIIa receptor” City-Wide Cardiology Rounds, University of Toronto (Nov 17, 1997)

16. “c-Myb-dependent vascular smooth muscle cell proliferation” Vascular Biology Seminars, Hospital for Sick Children, Toronto, ON (Dec 3, 1997)

17. “c-Myb-dependent vascular smooth muscle cell proliferation” Cardiology Rounds, Mt. Sinai Hospital, University of Toronto (April 15, 1997)

18. “c-Myb regulates vascular smooth muscle cell cycle progression” Scientific Day, Centre for Cardiovascular Research, University of Toronto (May 20, 1995)

2). National:

19. “Altering Ca2+ homeostasis in vascular smooth muscle: mouse models of hypertension” Visiting Professor, IRCM, Montreal (April 2, 2003)

20. “Regulated expression of PMCA4b in transgenic mice”. Research Seminar, Queen’s University, Kingston, ON (January 15, 2003)

21. “Transgenic models of hypertension and heart disease”. Research Seminar, University of Ottawa, ON (2002)

CV: Husain, M Page 21 of 22 06/20/2003

22. “Pathogenecity of inducible nitric oxide synthase: transgenic models” Cardiovascular Research Institute, St. Boniface General Hospital Research Centre, University of Manitoba (April 3, 2002).

23. “Regulated tissue-specific over-expression of iNOS in transgenic mice” Guest Speaker, University of Alberta Cardiac Sciences Day, Edmonton, Alberta (Jun 1, 2001)

24. “Transcriptional Regulation: The New Frontier of Molecular Medicine” Department of Medicine, University of Alberta, Edmonton, Alberta (May 31, 2001)

25. “How will vascular biology influence future therapy for coronary disease and stroke?” GUSTO IV ACS, Investigators Meeting, Toronto, ON (Sep 16, 2000)

26. “Vascular Biology: Past Present & Future Impacts” Visiting Professor, Department of Anesthesia, University of Calgary (Nov 23, 2000)

27. “Emerging trends in Acute Cardiac Care” Visiting Professor, Department of Anesthesia, University of Calgary (Nov 24, 2000)

28. “Conditional expression of iNOS in the mouse cardiovascular system” Symposium on Atherosclerosis, Department of Laboratory Medicine & Pathobiology, University of Toronto (Nov 23, 1999)

29. “Transgenic studies of vascular proliferation: the role of PMCA4” Visiting Professor, London Cardiovascular Society, Robarts Research Institute, London, Ontario (Mar 25, 1999)

30. “The role of c-Myb in vascular smooth muscle cell proliferation” Research Rounds, Montreal Heart Institute, Montreal (Feb 11, 1999)

31. “Transgenic regulation of vascular smooth muscle: Overexpression of PMCA” Visiting Professor, Department of Pharmacology, University of Montreal (Feb 10, 1999)

32. “c-Myb-dependent vascular smooth muscle cell proliferation” Research Seminars, Robarts Research Institute, University of Western Ontario, London, ON (1997)

33. “Overexpression of plasma membrane Ca2+ ATPase inhibits vascular smooth muscle cell proliferation” Cardiovascular Research Seminars, University of Calgary, Calgary, AB (1996)

34. “c-Myb-dependent vascular smooth muscle cell proliferation” Cardiovascular Research Rounds, University of Alberta, Edmonton, AB (1995)

3). International:

35. “Tissue-specific conditional gene expression: a tool enabling unique models of cardiovascular disease” Scheduled Invited Speaker, 2004 Angiotensin Gordon Conference, Ventura, CA (Feb 29-Mar 5, 2004)

36. “Diagnosis MD” Episodes 4021, 4022, 4023, 4133, 4134, 4135. The Life Network, Alliance Atlantis Studios, Toronto, Ontario (June 14, & July 24, 2003)

37. “Novel genetic and clinical insights in congestive heart failure” Chair of Plenary Session, American College of Cardiology International Heart Failure Summit, Toronto, ON (June 12, 2003)

38. “Role of c-Myb in vascular smooth muscle cell development, differentiation, and disease” Scheduled Invited Speaker, Myb 2003, Oxford University, Oxford, UK (Apr 5-8, 2003)

39. “Mouse models of hypertension: tools, tricks & surprises” Canadian Hypertension Society: Basic Science Breakfast (Oct 28, 2002)

40. “Over-expression of iNOS: A Mouse Model of Heart Failure and Heart Block” Featured Session: Genetic Forms of Cardiomyopathy, World Congress of Pediatric Cardiology, Toronto, ON (May 28, 2001)

CV: Husain, M Page 22 of 22 06/20/2003

41. “New Strategies for Selective Targeting of Transgenes to Vascular Smooth Muscle and for Achieving Conditional Expression” Featured Lecture of Sunday Morning Sessions. Cardiopulmonary Knockout Mice: Lessons from Existing Mice and Strategies for Tissue Specific Targeting and Conditional Expression, Gene Expression/Regulation, American Heart Association Scientific Sessions, New Orleans, LA (Nov 11, 2000)

42. “Mouse models of arterial injury” Symposium on Animal Models of Human Disease, Canadian Society for Atherosclerosis and Vascular Biology, Quebec City, PQ (Oct 21, 1999)

43. “Regulating vascular smooth muscle cell physiology: lessons learned from transgenic mice” 10th Annual R. W. Gunton Symposium on Therapeutics, Robarts Research Institute, London, ON (Sep 10, 1999)

44. “Conditional transgene regulation in arterial smooth muscle cells in mice” Scientific Sessions, American Heart Association, Orlando, FL (Nov 7, 1997)

45. “Overexpression of plasma membrane calcium ATPase-1 reduces intracellular calcium and slows growth of vascular smooth muscle cells” Scientific Sessions, American Heart Association, New Orleans, LO (Nov 10, 1996)

46. “Dominant negative c-Myb constructs lower intracellular calcium levels and slow cell cycle progression in vascular smooth muscle cells” Scientific Sessions, American Heart Association, Atlanta, GA (Nov 8, 1995)

47. “Transcription factors involved in vascular smooth muscle cell proliferation” Joint Symposium of the Medical Research Council of Canada and the Canadian Society of Clinical Investigation, Montreal, PQ (Sep 13, 1995) (Winner of a Travel award)

48. “Dominant negative c-Myb constructs lower inhibit cell cycle progression through dysregulated Ca2+ homeostasis in vascular smooth muscle cells” Symposium on Molecular Medicine, Harvard Medical School (Apr 15, 1995)

49. “Endothelial necrosis and basic FGF: early features of neointimal formation” Scientific Meeting, Canadian Cardiovascular Society, Vancouver, BC (Oct 10, 1993)

50. “An escape phenomenon of lipoprotein(a) in sustained plasma exchange” Scientific Meeting, American Federation for Clinical Research, New York City, NY (Winner of an AFMR Trainee Award) (Apr 23, 1992)