1. Polyclonal Antibody Development Service

MTR offering a variety of Polyclonal Antibody Services to fit your research and production

needs. we develop polyclonal antibodies to targets including parasites, cancer-specific

surface antigens, hormones, growth factors and their receptors, glycoproteins, peptides,

proteins and their modification sites (mutation site-specific, phosphorylation site-specific,

cleavage site-specific, etc), and other antigens such as small-molecule compounds and DNA.

We have created antibodies for various applications, such as functional assays, antibodies for

immunoprecipitation, IHC, IF, flow cytometry, western blot, and EIA.

Project Timeline:

4-6 weeks

Deliverables:

We will deliver approximately 1 ml of mice serum or 5 ml of Rabbit serum with guaranteed

titer at least 1:10,000 (Range 1:10,000 to 1:100,000).

2. Monoclonal Antibody Development Services

MTR offer mouse hybridoma development technology for producing custom monoclonal

antibodies from synthesized peptide or recombinant protein antigens. Novel immunization

strategies, antigen design, and screening technologies are essential for the successful

generation of monoclonal hybridoma cells lines. Our immunization protocols take advantage

of using altered immunization routes, combinations of ligand carriers and dosing strategies to

produce the most diverse monoclonal antibody population possible. Antigens must be

carefully designed, especially when dealing with peptides, to generate a robust reagent that is

able to recognize conformational changes of the native protein in assay conditions.

Highlights:

Monoclonal antibodies - Robust protocol options for immunizing mice, ELISA-titer test

bleeds, creating fusions, screening hybridomas, and delivering cell lines

Customizable and adaptable - each protocol can be expanded or modified in a variety of

ways to service specific needs for final scale, specificity or assay validation

Project Timeline:

3-4 months

Deliverables:

We will deliver two positive Hynridoma clone and 5ml of culture supernatant.

3. Antibody and Protein Purification Services

Services and Offerings

MTR provide purification service antibodies and protein utilizing a wide array of protein

purification methods; from research (mg) to scale-up (grams) quantities. We also offer low

endotoxin production for in-vivo use. Our goal is to find or develop the antibody purification

procedure which provides the best balance between yield, purity, and cost. Available

Antibody Purification services and methods include:

Ammonium Sulphate Precipitation

This method is useful for concentration and partial purification of antibodies from most

sources and all species. Although, on its own the yields of antibody are impure, it is the

method of choice when combined with ion exchange for large volumes of anti-sera. It is not

recommended for purification of tissue culture supernatant.

Protein A

This process binds the Fc region of major sub-classes of mouse and rat IgG to varying

degrees. The results with protein A are usually high purity and high yields. It is useful for

large-scale purifications of tissue culture supernatants.

Protein G

Protein G can be used for purifying antibody from most sources. Like protein A, the results

are usually of high purity and high yield. It is applicable to a wider range of

immunoglobulins than protein A, but elution procedures can be much harsher.

Ion Exchange Chromatography

This method is used for all sources of antibodies. It is applicable to all lgG isotypes. It is

usually the method of choice when purifying antibodies from ascites, as the monoclonal can

be resolved from the host immunoglobulins.

Antigen Affinity

This is the method of choice when specific antibody is required from polyclonal anti-sera. It

requires pure antigen for the best results and can sometimes result in inactive antibody.

Size Exclusion

This method is appropriate for IgM antibodies from all sources. It is used when IgM

Antibodies need to be separated from polyclonal anti-sera. It is not recommended as a single

stage.

Project Timeline:

2-3 weeks

Deliverables:

Purified antibody

Project report

4. Immunoassay Development Services

MTR immunoassay development services are project-based and designed for diagnostics and

drug discovery applications. Our scientists and project managers will discuss with each client

the project design, cost estimates, and delivery specifications.

We offer three basic categories of services

Immunoassay development service for diagnostic Kits.

Development and Validation of ELISA of ELISA for pharmacokinetic assay of Bio

similar protein, Antibody and peptide.

Development and Validation of ELISA for Immunogenicity assay for Bio similar protein,

antibody and peptide

Project Timeline:

3-4 months

Deliverables:

Deliverables vary depending on the types of services requested and the requirements of

the assays.

In general, we deliver cell lines, antibodies and conjugates, assay protocols, assay

reagents, assay kits, experiment records, and specific data derived from a given project.

5. Labeling of Antibody and Protein

MTR offer conjugation service for antibody, protein and peptide with following conjugate

Traditional fluorophores such as FITC and Texas Red® dye

Biotin

HRP

Alkaline phosphatase, and other enzymes

KLH and BSA for peptide

If you don’t see the label you are interested in, just ask! In most cases we can accommodate

your request.

Sample requirements:

Synthetic peptide or amino acid sequence of the peptide

Project Timeline:

2-3 weeks

Deliverables:

Peptide carrier conjugate

Project report

6. Molecular and cell Biology Services

MTR offer reliable and economical molecular biology outsourcing, including de novo gene

synthesis, gene cloning, DNA construct, plasmid preparation, and site directed mutagenesis.

The services cover the entire range of gene analysis from identification and cloning to

functional studies.

Deliverables:

Cloned gene in desired vector.

7. Cell-based Bioassay Services

MTR offer service for developing the cell based assays for the determination of specific

activity and biological characterization. We have expertise and technology for the following

assay

Cell Proliferation Assays

Cell Based cAMP Release Assay

Competitive Receptor Binding (Flow Cytometry)

Reporter Gene Assay

CDC Assay

ADCC Assay

Antiproliferation Assay

Immunogenecity Assays

Neutralizing Antibody Assays

Deliverables:

Project report