Regina Dönen Am Kirchberg 43, 79539 Lörrach r.doenen@hotmail.com

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! PERSONAL DETAILS Date of Birth 19 June 1988 Place of Birth Balchash ( Kazakhstan) Nationality German Marital Status Married, 2 children Maiden Name Agarkow ! WORK EXPERIENCE

University Hospital Basel 04/2018 – Present Research Lab Technician 60% at the Department of

Biomedicine ( Prof. Dr. A. Zippelius)

Field of Research: Cancer Immunology Genotyping, PCR

Cell work In-vivo work on mice: tumor measurements, compound administration s.c., i.p, i.v., p.o., i.m ; blood-, tissue- and organ sampling, tumor transplantation or cell injections

Legacy Pharmaceuticals Switzerland, Birsfelden

06/2017 – 03/2018 Lab Technician Microbiology

Environmental Monitoring in the sterile/non sterile production Identification of microorganism

Editing and reviewing of SOPs , instructions and deviations

Regina Dönen Am Kirchberg 43, 79539 Lörrach r.doenen@hotmail.com

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University of Basel 03/2017 – 05/2017 Research Lab Technician 50% at the Department of

Biomedicine – temporary ( Prof. Dr. V. Taylor & R. Zeller)

Field of Research: Embryology and Stem Cell Biology / Development Genetics Assisting 2 research groups in general lab work

( Genotyping, PCR, qPCR ) 03/2016 – 02/2017 Research Lab Technician 60% at the Biozentrum

(Prof. Dr. Jean Pieters) Field of Research: Infection Biology / Biochemistry

Work on cells ( cell cultivation, freeze and defrost cells) FACS, Western Blot, PCR & RT-PCR Proteinquantification ( BCA,Bradford) In-vivo work on laboratory mice ( compound administration i.p, s.c. ; tissue, organs & blood sampling Documentation of the experiments Provide general administrative support

01/2014 – 02/2016 Research Lab Technician 50% at the Department of

Biomedicine – temporary (Prof. Dr. Verdon Taylor)

Field of Research: Embryology and Stem Cell Biology ; Cancer Project Publication: Cancer Cell: “A Tumor Suppressor Function for Notch

Signaling in Forebrain Tumor Subtypes” Work on cells ( cell cultivation, freeze and defrost cells)

Western Blot, PCR & RT-PCR, DNA-/RNA-/ Proteinextraction Genotyping Virus transfection, retroviral particles production In-vivo work on laboratory mice ( mice brain harvesting, intracardial brain perfusion) Paraffin- and frozen cuts, immunohistochemical & immunofluorescent staining, light- and fluorescent microscopy Documentation of the experiments

Regina Dönen Am Kirchberg 43, 79539 Lörrach r.doenen@hotmail.com

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F. Hoffmann-La Roche in Basel 11/2011 – 12/2013 Technician Quality-Control 100% (temporary)

Environmental Monitoring in the production of parenterals Extensive experience in GMP/GLP- Environment, editing and reviewing of standard operation procedures (SOPs), Validation of lab equipment, Management of analytical results

! SCHOOL/ EDUCATION 2014 FACS ( fluorescence activated cell sorting ), Germany 2012 Environmental monitoring in pharmaceutical industry,

Germany

Apprenticeship

2008 – 2011 Training as Biology Lab Technician in Novartis Pharma AG in Basel

Division: Novartis Institutes of Biomedical Research Field of the Practical Trainings: Muscle diseases area Musculoskeletal diseases Osteoporosis Magnetic resonance imaging ( MRI ) Autoimmunity, Transplantation and Inflammation Diseases

2005 – 2008 Science Gymnasium Mathilde Planck-Schule in Lörrach, Germany

Qualifications: „A“ – Level (Abitur) ! LANGUAGES German Fluent English Upper Level (spoken and written) French Intermediate Level Russian Native Language